Supplementary material
Effect of synthetic 2’,4’,5’-trimethoxychalcones on apoptotic pathways in human K562 and Jurkat leukemia cells
Aline Costa, Louise Domeneghini Chiaradia-Delatorre, Lorena dos SantosBubniak, Alessandra Mascarello, Marley Aparecida Licínio Marzarotto, Ana Carolina Rabello de Moraes, Taisa Regina Stumpf, Marlon Norberto Sechini Cordeiro, Rosendo Augusto Yunes,Ricardo José Nunes, Maria Cláudia Santos-Silva
1.Cytotoxic effect of chalcones 13, 16, 19 and 26 on mononuclear cells (normal human lymphocytes) of healthy individuals. The chalcones were added to the mononuclear cells of healthy individuals (2.5 x 106 cells/mL) at their respective IC50values and incubated for 24 h. Cell viability was assessed by the MTT method. The optical density of the control group (C) cells with no treatment was considered as 100% of cell viability. Two controls were used: a group treated with 30 µM taxol and a group treated with 2% DMSO vehicle in which the chalcones were diluted. Each point represents the mean ± SEM. *p<0.05 compared with control group (ANOVA followed by Bonferroni’s t-test).
1H NMR and 13C NMR spectra of novel synthesized compounds
1H NMR spectra of compound 4:
13C NMR spectra of compound 4:
1H NMR spectra of compound 5:
13C NMR spectra of compound 5:
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13C NMR spectra of compound 7:
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13C NMR spectra of compound 8:
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13C NMR spectra of compound 9:
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13C NMR spectra of compound 12:
1H NMR spectra of compound 16:
13C NMR spectra of compound 16:
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13C NMR spectra of compound 17:
1H NMR spectra of compound 19:
13C NMR spectra of compound 19:
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13C NMR spectra of compound 22:
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13C NMR spectra of compound 24:
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1H NMR spectra of compound 26:
13C NMR spectra of compound 26:
1H NMR spectra of compound 27:
13C NMR spectra of compound 27:
1H NMR spectra of compound 28:
13C NMR spectra of compound 28:
1H NMR spectra of compound 29:
13C NMR spectra of compound 29: