Edwin J. Squirewell

816 2nd ST NW Apt 1, Rochester, MN 55901

; 843-478-5974

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RESEARCHEXPERIENCE

Mayo Clinic,Rochester, MN: Postdoctoral Research Fellow, 2014 – 2016

Role of Focal Adhesion Kinase and Cell Adhesion Remodeling in Melanoma

  • Aseptic laboratory techniques.
  • Mammalian cell culture and maintenance of multiple cell lines.
  • Transfections using siRNA and lentivirus.
  • Cell proliferation, migration, and invasion assays using live-cell imaging (IncuCyte ZOOM).
  • Cell/tissue section immunohistochemistry using automated instrumentation (BOND-MAX).
  • Confocal microscopy and flow cytometry.
  • Utilized luciferase reporter assay in 96-well format to screen for human kinases involved in the expression of osteopontin (Spp1), a protein overexpressed in melanoma that is strongly associated with cancer invasion and metastasis.
  • Bacterial cell culture, DNA cloning, PCR,qPCR, sequencing, and mutagenesis.
  • DNA/RNA isolation from cells/tissues using QIAGEN kits and automated standard protocols (QiaCube).
  • Western blot
  • Automated western blot (ProteinSimple Wes).
  • Automated pipetting (epMotion).
  • Maintained, genotyped, and bred genetically modified mouse colonies.
  • Tumor xenograftimplantation techniques (flank, intradermal, subcutaneous, intracardiac).
  • Bioluminescence and fluorescencein vivo xenogen imaging (IVIS).
  • Harvested tumor xenografts for cell culture.
  • Mouse dissection and tissue collection.
  • Performed injections and administered drugs in mice (gavage, intraperitoneal).
  • Maintained inventory supply, performed routinemaintenance on laboratory equipment,trained and mentored new personnel.

University of Iowa,Iowa City, IA: Graduate Research Fellow, 2009-2014

Interactions of Tamoxifen Metabolites with Human Cytosolic Sulfotransferase: Implications for the Therapeutic Action and Toxicity of Tamoxifen

  • Developed bacterial expression systems for recombinant protein production.
  • Protein purification using ion exchange and affinity column chromatography.
  • PCR, gel electrophoresis, sequencing.
  • DNA isolation using QIAGEN kits.
  • Protein analysis bySDS-PAGE and western blot.
  • HPLC experience.
  • Experience using radioactive materials (3H and 35S)
  • Developed in vitro enzyme kinetic assays to determined mechanism of inhibition and related kinetic constants (IC50, Vmax, Km, Kcat, Ki, product inhibition, alternate substrates).
  • UV spectroscopy (colorimetric enzyme assays).
  • Synthetic organic chemistry techniques: thin layer chromatography, flash column chromatography, rotary evaporation, lyophilization.
  • Structure elucidation using proton NMR and LC-MS.
  • Managed compressed gas and radiochemical inventory.
  • Oversaw radiochemical disposal and radiation safety protocols.
  • Oversaw annual radiochemistry audits.

Charleston Southern University, Charleston, SC: Undergraduate Research Fellow, 2007-2009

The Catalytic Mechanism of Recombinant & Native Sialyltransferase

  • Conducted research with the goal of understanding the catalytic mechanism of mammalian sialyltransferases.
  • Utilized site-directed mutagenesis to generate mutant enzyme construct.
  • Cloned, overexpressed, purified, and kinetically characterized recombinant proteins in bacterial and insect cells.

Professional Skills

  • Strong organizational, scientific, and computer skills (e.g., Microsoft Office, GraphPad Prism, Sigma Plot, ChemDraw, Vector NTI).
  • Extensive experience writing manuscripts and presenting research data.
  • Effective problem solver with a keen eye for detail.
  • Significant experience training and mentoring laboratory personnel.
  • Able to work independently and as part of a team.
  • Self-motivated, adaptable, able to learn new techniquesand perform multiple tasks simultaneously.

EDUCATION

  1. Ph.D. (2009 – 2014), University of Iowa

Pharmacy (Medicinal and Natural Products Chemistry)

  1. B.S. (2005 – 2009), Charleston Southern University

Biochemistry

SELECTEDPUBLICATIONS

  1. Meves, A., Nikolova, E., Heim, B.J., Squirewell, E. J., Cappel, M.A., Pittelkow, M.R., Otley, C.C., Behrendt, N., Saunte, D.M., Andersen, J.L., Schenck, L.A., Weaver, A.L., Suman, V.J., (2015) “Tumor cell adhesion as a risk factor for sentinel lymph node metastasis in primary cutaneous melanoma.” Journal of Clinical Oncology. 33(23): 2509-2515
  1. Squirewell, E. J., and Duffel, M.W. (2015) “The Effects of Endoxifen and Other Major Metabolites of Tamoxifen on the Sulfation of Estradiol Catalyzed by Human Cytosolic Sulfotransferases hSULT1E1 and hSULT1A1*1.” Drug Metabolism and Disposition. 43(6): 843-850
  1. Squirewell, E. J., Qin, X., and Duffel, M.W. (2014) “Endoxifen and Other Metabolites of Tamoxifen Inhibit Human Hydroxysteroid Sulfotransferase 2A1 (hSULT2A1).” Drug Metabolism and Disposition. 42(11): 1843-1850
  1. Squirewell, E. J., "Interactions of Endoxifen and other major metabolites of Tamoxifen with human sulfotransferases SULT2A1, SULT1E1, and SULT1A1*1: implications for the therapeutic action and toxicity of Tamoxifen." PhD (Doctor of Philosophy) thesis, University of Iowa, 2014.

AWARDS ANDRECOGNITION

2014 Mayo Clinic Dermatology Research Fellowship

2013Trainee Member of the Holden Comprehensive Cancer Center in Cancer Signaling and Experimental Therapeutics

2013FASEB/MARC Travel Award to Experimental Biology 2013

2012 American Society for Pharmacology and Experimental Therapeutics (ASPET)

2012 Joseph G. Cannon Excellence in Graduate Research Travel Award

2009University of Iowa Graduate Dean’s Fellowship Recipient

2009 Charleston Southern University Chemistry Award

2009 Beta Beta Beta National Biological Honor Society

2008 South Carolina Independent Colleges and Universities Faculty/Student Undergraduate Research Grant

2005 South Carolina Physics Scholars Institute

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