Donald D. Ruhl, Jr., Ph.D. Curriculum Vitae
Donald D. Ruhl, Jr., Ph.D.
Department of Biochemistry and Molecular Biology
Oklahoma State University
348F Noble Research Center
Stillwater, Oklahoma 74078
Office: 405-744-6409
Fax: 405-744-7799
E-mail:
CURRENT POSITION
Oklahoma State University, Stillwater, Oklahoma
Assistant Professor, February 2011 to Current
Academic Advisor
Department of Biochemistry and Molecular Biology
EDUCATION
Cornell University, Ithaca, New York
Postdoctoral Fellow, July 2005 to June 2010
Department of Molecular Biology and Genetics
Principal Advisor: Dr. W. Lee Kraus
Saint Louis University School of Medicine, Saint Louis, Missouri
Doctor of Philosophy, July 2005
Department of Pharmacological and Physiological Sciences
Principal Advisor: Dr. John C. Chrivia
Thesis Title: Protein Kinase A, the Estrogen Response Element and a Chromatin Remodeling Complex: Two Mechanisms in Gene Regulation.
University of Missouri – Saint Louis, Saint Louis, Missouri
Bachelor of Arts in Chemistry, December 1993
Principal Advisor: Dr. William Welsh
Research studies in drug molecular modeling.
TEACHING EXPERIENCE
Oklahoma State University, Stillwater, Oklahoma
Survey of Biochemistry, BIOC 3653, 3 credit hours. Fall 2010 - Current
Course is for upper level undergraduate students (approx. 165 students/fall semester)
Biochemical Laboratory, BIOC 3723, 3 credit hours, Fall 2010 - Current
Course is for upper level undergraduate students (approx. 24 students/semester)
Biochemical Laboratory Methods, BIOC 5824, 4 credit hours, Fall 2010 - Current
Course is for graduate students (1 student/semester)
Teaching Internship, BIOC 4990, 3 credit hours, Fall 2010 - Current
Teaching experience for undergraduates (1 student/fall semester)
Chemical Application of Biomolecules, BIOC 2344, 4 credit hours, Spring 2011 - Current
Course is for undergraduate non-majors (approx. 30 students/spring semester)
Special Problems, BIOC 4990, variable credit hours, Spring 2011 – Current
Research experience for undergraduates (approx. 9 students/semester)
Cornell University, Ithaca, New York
Undergraduate Research Mentor, January 2009 to May 2010
Student: Arunabha “Sunny” Basu. Sunny is investigating the role of PARP-1 in the inhibition of Swi/Snf chromatin remodeling.
Guest Lectures, Course: Molecular Basis of Human Disease, Fall 2009
Course is for upper level undergraduate and graduate students. (approx. 80 students)
Student: Ehsan Balagamwala. Ehsan’s Senior Research Thesis, Biochemical Analyses of Poly(ADP-Ribose) Polymerase-1 Interactions and Functions on Chromatin Templates, received Highest Honors from Cornell University.
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Teaching Assistant, Gene Expression Course 2008 - 2010
Taught biochemical methods for studying transcriptional regulation to graduate students and postdocs. (Total Students: 16)
Saint Louis University School of Medicine, Saint Louis, Missouri
Undergraduate Research Mentor, January 2003 to May 2005
Student: Jennifer Jimenez. Jennifer studied the role of PKA in the regulation of ERE containing genes.
Teaching Assistant, Physiology, Fall 2004
This course was part of a program designed to give disadvantaged students an opportunity to strengthen their application to medical school. (Total Students: 12)
Princeton Review, Saint Louis, Missouri
Instructor, MCAT Chemistry, Spring 2002
Taught students material equivalent to the first two college semesters of General Chemistry. (Total Students: 40)
PUBLICATIONS
Ruhl DD, Forth S, Wang MD, Kraus WL. The Estrogen Receptor Modulates the Activity of Swi/Snf. (in preparation).
Ruhl DD, Basu A, Kraus WL. Poly ADP-Ribose Inhibits the Remodeling Activity of Swi/Snf. (in preparation).
Zhang T, Berrocal JG, Yao J, Dumond ME, Krishnakumar R, Ruhl DD, Ryu KW, Gamble MJ, Kraus WL. 2012. Regulation of Poly(ADP-ribose) Polymerase-1-dependent Gene Expression through Promoter-directed Recruitment of a Nuclear NAD+ Synthase. J Biol Chem. 287:12405-16.
Langelier MF, Ruhl DD*, Planck JL, Kraus WL, Pascal JM. 2010. The Zn3 Domain of Human Poly(ADP-Ribose) Polymerase-1 (PARP-1) Functions in Both DNA- dependent Poly(ADP-Ribose) Synthesis Activity and Chromatin Compaction. J Biol Chem. 285:18877-87.
* Co-first Author
Hah N, KolkmanA, RuhlDD, PijnappelWW, HeckAJ, TimmersHT, Kraus WL. 2010. A Role for BAF57 in Cell Cycle-Dependent Transcriptional Regulation by the SWI/SNF Chromatin Remodeling Complex. Cancer Research. 70:4402-11.
Ruhl DD, Kraus WL. 2009. Biochemical Analyses of Nuclear Receptor-Dependent Transcription with Chromatin Templates. In: Chakravarti D (ed.), Regulatory Mechanisms in Transcriptional Signaling: Transcriptional and Physiological Control by Nuclear Hormone Receptors, (series: Progress in Molecular Biology and Translational Science, Volume 87) Elsevier, pp. 138-193.
Wacker DA, Ruhl DD*, Balagamwala EH, Hope KM, Zhang T, Kraus WL. 2007. The DNA Binding and Catalytic Domains of Poly(ADP-Ribose) Polymerase 1 Cooperate in the Regulation of Chromatin Structure and Transcription. Mol Cell Biol. 27:7475-85.
* Co-first Author
Ruhl DD, Jin J, Cai Y, Swanson S, Florens L, Washburn MP, Conaway RC, Conaway JW, Chrivia JC. 2006. Purification of a Human SRCAP Complex that Remodels Chromatin by Incorporating the Histone Variant H2A.Z into Nucleosomes. Biochemistry. 45:5671-5677.
Monroy MA, Ruhl DD, Xu X, Granner DK, Yaciuk P, Chrivia JC. 2001. Regulation of cAMP-Responsive Element-Binding Protein-Mediated Transcription by the SNF2/SWI-related Protein, SRCAP. Journal of Biological Chemistry. 276:40721-40726.
Ruhl DD, Pusateri ME, Eliceiri GL. 2000. Multiple Conserved Segments of E1 Small Nucleolar RNA are Involved in the Formation of a Ribonucleoprotein Particle in Frog Oocytes. Biochemical Journal. 348:517-524.
Doub WH, Ruhl DD, Hart B, Mehelic PR, Revelle LK. 1996. Gradient Liquid Chromatographic Method for Determination of Chlorhexidine and its Degradation Products in Bulk Material. Journal of AOAC International. 79:636-639.
ABSTRACTS/PRESENTATIONS AT NATIONAL CONFERENCES
Ruhl DD, Forth S, Wang MD, Kraus WL. 2010. Estrogen Receptor-Dependent Modulation of SWI/SNF Activity. Keystone Symposia.
Gibson BA, Ruhl DD, Krishnakumar R, Basu A, Kraus WL. 2010. Regulation of Chromatin Structure and Gene Expression by Poly(ADP-ribose) polymerase-1. Keystone Symposia.
Ruhl DD, Kraus WL. 2009. Estrogen Receptor-Dependent Modulation of SWI/SNF Activity. Keystone Symposia.
Ruhl DD, Hah N, Kolkman A, Pijnappel P, Timmers MH, Heck A, Kraus WL. 2007. Role of the Baf57 and Baf60a Subunits of Human SWI/SNF in Estrogen Receptor-mediated Gene Expression. FASEB.
Ruhl DD, Hah N, Kraus WL. 2006. Role of the SWI/SNF Chromatin Remodeling Complex in Estrogen Regulated Transcription. Keystone Symposia.
Ruhl DD, Ruh MF, Chrivia JC. 2004. Activation of the Estrogen Response Element by PKA Independent of the Estrogen Receptor. Endo 2004.
Ruhl DD, Chrivia JC. 2004. Transcriptional Activation by SRCAP, and the Purification of a SRCAP Complex. Endo 2004.
Ruhl DD, Chrivia JC. 2003. Activation of Estrogen Response Elements by Protein Kinase A. Jensen Symposium.
Monroy MA, Ruhl DD, Yaciuk P, Chrivia JC. 2002. SRCAP Enhances Glucocorticoid Receptor-Regulated Gene Expression. Endo 2002.
Grosso LE, Ruhl D, Paterson A. 2001. Analysis of Polymorphisms/Mutations in Coagulation Associated Proteins in a High Risk Obstetric Practice. Association for Molecular Pathology 2001.
GRANTS
CASNR Student Tech Fee, 2011, Two Liquid Chromatography Units and a Computer. Awarded $19,587.
FELLOWSHIPS
Postdoctoral Fellowship. Recruitment and Activation of the Swi/Snf Chromatin Remodeling Complex at Estrogen Receptor-regulated Promoters in Breast Cancer Cells. NIH Training Grant, Department of Pharmacology, Weill Cornell Medical College. May 1, 2009 to April 30, 2010. Grant Number: 5T32 CA062948-14.
Postdoctoral Fellowship. NRSA-NIDDK. June, 2006 - June, 2009. Reference Number: F32 DK076275-01. (Declined)
Postdoctoral Fellowship. American Heart Association – Northwest Affiliate. July 1, 2006 to June 30, 2008. Reference Number: 0625893T. (Declined)
Postdoctoral Fellowship. Recruitment and Activation of the SWI/SNF Chromatin Remodeling Complex at Estrogen Receptor-regulated Promoters in Breast Cancer Cells. Susan G. Komen Breast Cancer Foundation. May 1, 2006 to April 30, 2009. Grant Number: PDF0601371.
Predoctoral Fellowship. NIH Training Grant, Department of Pharmacological and Physiological Science, Saint Louis University. July 1, 2004 to June 30, 2005. Grant number: 5T32GM008306-15.
Predoctoral Fellowship. Regulation of Estrogen Receptor Mediated Transcription by the SNF2-Related Protein, SRCAP. American Heart Association-Heartland Affiliate. July 1, 2002 to June 30, 2004. Grant Number: 0215232Z.
AWARDS
CASNR Outstanding Faculty Advisor, 2013
Keystone Symposia Scholarship, 2010. Estrogen Receptor-dependent Modulation of Swi/Snf Activity.
First Place, Sigma Xi, 2004. Activation of the Estrogen Response Element by Protein Kinase A Independent of the Estrogen Receptor.
Third Place, St. Louis University Graduate Student Research Symposium, 2004. Activation of the Estrogen Response Element by Protein Kinase A.
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