STable 1 Comparison of the intracellular metabolites of Candida versatilis cultivated under free-salt, 9% and18% salt environment, respectively.

OS-GC-MS-PCA1 / OS-GC-MS-PCA2
Metabolites / Relative peak area / Metabolites / Relative peak area
0% NaCl / 9% NaCl / 18% NaCl / 0% NaCl / 9% NaCl / 18% NaCl
Etanol; / 0.013 / 0.025 / 0.029 / Propylene glycol / 0.299 / 0.381 / 0.234
3-Methylthiopropanol; / 0.0769 / 0.071 / 0.0253 / Benzenemethanol, a-2-propen-1-yl- / 2.012 / 5.792 / 1.451
Glycol / 0.017 / 0.048 / 0.042 / Butanoic acid,2-hydroxy-; / 0.22 / 0.14 / 0.21
2,3-Butanediol / 0.054 / 0.045 / 0.028 / Malonic acid / 0 / 0.25 / 0
1,2-butylene glycol / 0.008 / 0.009 / 0.099 / Acetic acid / 0.23 / 0 / 0.19
4-Hydroxyphenethyl alcohol / 0.092 / 0.066 / 0.0344 / 3-Aminoisobutyric acid / 2.04 / 2.741 / 1.8302
Isopropanol / 0.27 / 1E-04 / 0.025 / Succinic acid; / 0.23 / 1.344 / 0.3
Isobutyl alcohol; / 0 / 0.14 / 0.12 / Fumaric acid;Maleic acid; / 0.084 / 0.758 / 0
Glycerol / 3.667 / 1.751 / 0 / Glutaric acid; / 0.006 / 0.027 / 0
1-Butanol; / 0 / 0.141 / 0.167 / 4-Hydroxy-3,5-dimethoxycinnamic acid;Sinapic acid / 0.08 / 0.12 / 0.03
3-Methyl-1-butanol;Isoamyl alcohol; / 0 / 0.25 / 0.23 / Oleic acid / 0.05 / 0.04 / 0.08
Diglycolic / 0.11 / 2.04 / 3.73 / Palmitic acid;Hexadecanoic acid / 0.568 / 0.885 / 0.725
3 - hydroxy acid / 0.97 / 0.67 / 0.06 / 2-Aminobutyric acid / 0.016 / 0.093 / 0
2 - glycolic acid / 0.07 / 2.76 / 1.87 / Ribonic acid gamma-lactone; / 0.34 / 1.21 / 0.21
Glycolic acid; / 0.11 / 0.25 / 0.23 / Glycerophosphoric acid / 0.04 / 0.03 / 0.04
Pyruvic acid / 0.02 / 0.25 / 0.22 / Gluconic acid lactone; / 0.17 / 0.11 / 0.22
DL-2-Hydroxycaproic acid / 1.09 / 0.019 / 0 / Thymol / 1.27 / 2.38 / 1.1
erythro-Pentonic acid / 0.97 / 0.314 / 0.1402 / Glycine / 0.12 / 0.03 / 0.33
Glyceric acid / 0.047 / 0.071 / 0.068 / Norvaline / 0.2 / 0.25 / 0.22
Benzoic acid / 1.11 / 0.023 / 0.0224 / Tyrosine; / 1.046 / 3.7272 / 1.8634
Lactic acid / 0.468 / 0.874 / 1.5707 / a-DL-Xylopyranose/a- / 0 / 0.06 / 0
Malic acid / 0.065 / 0.154 / 0.138 / ribo-Pentitol / 2.97 / 2.04 / 9.43
Citric acid; / 0 / 0.27 / 0.25 / Lactose;D-(+)-Lactose; / 0 / 0.1078 / 0
hexadecanedioic acid / 0 / 0 / 0.25 / Lactulose;4-O-beta-D-Galactopyranosyl-alpha-D-fructose / 2.04 / 3.73 / 0.25
2-(2-Furyl)glycolic acid / 0.25 / 0.22 / 0 / Ribofuranose / 0.97 / 0.08 / 9.43
2-Ketoglutaric acid; / 0.14 / 0.21 / 0.34 / Mannose / 0.07 / 0.14 / 0.06
3-Phenylpyruvic acid; / 0 / 0.856 / 0.9709
Tartaric acid; / 0 / 0.21 / 0.23
Lauric acid; / 0 / 0.43 / 0.62
Stearic acid; / 0.113 / 0.217 / 0.185
Gluconate / 0.05 / 0.06 / 0.07
Butyl hydroxyacetate / 0.12 / 0.15 / 0.23
D-gluco-Heptonic acid, g-lactone, (2x)-; / 0 / 0 / 0.19
Galactopyranoside, methyl, 4-acetate, D- (8CI) / 0.22 / 0.01 / 0.02
D-gluco-Heptonic acid, g-lactone, (2x)-; / 3.57 / 0.43 / 0.32
L-(+)-Gulono-1,4-lactone / 2.11 / 1.35 / 0.23
Mannono-1,4-lactone / 10.18 / 3.22 / 1.26
Glycerin fatty acid ester / 0.26 / 0.11 / 0.11
1,3-Dihydroxyacetone / 0.02 / 0.31 / 0.56
Glyceraldehyde / 0.01 / 1.25 / 2.8
Alanine; / 0.12 / 0.06 / 0
Valine / 1.35 / 0.48 / 0.34
Serine / 1.6 / 0.14 / 0.21
Threonine / 0.73 / 0.25 / 0.29
Aspartic acid; / 1.06 / 1.02 / 0
Phenylalanine / 1.87 / 1.12 / 0.01
a-D-Galactopyranose, 1-(dihydrogenphosphate); / 0 / 0 / 0.97
5-Deoxy-D-Ribose; / 4.598 / 0.728 / 0
2-Deoxy-D-ribose / 2.047 / 0.903 / 0
Mannopyranose, 6-deoxy-; / 3.87 / 0.12 / 0.01
Galactopyranoside, methyl, 4-acetate, D- (8CI) / 6.23 / 0.12 / 0.07
a-D-Ribofuranose / 0 / 0 / 0.12
Methyl beta-D-ribofuranoside; / 0.085 / 0.899 / 7.7896
a-D-Arabinofuranose; / 3.54 / 40.13 / 25.56
Xylitol; / 3.01 / 3.01 / 9.43
Arabinitol / 3.11 / 1.1 / 0.6
D-Ribose/D(+)-Xylose/DL-Arabinose / 2.07 / 1.08 / 0.06
a-L-Galactopyranose, 6-deoxy-; / 3.11 / 0.14 / 0.67
Glucose / 5.046 / 0.527 / 0.032
a-D-Arabinopyranose / 1.14 / 0 / 0.21
alpha-D-Methylglucoside;Methyl-alpha-D-glucopyranoside / 1.33 / 0.03 / 0.14
a-D-Galactopyranoside, b-D-fructofuranosyl / 3.67 / 0.06 / 0.02
D-Fructose, 4-O-a-D-Galacopyranosyl- / 2.76 / 1.87 / 0.12
Maltose / 1.25 / 0.23 / 0.12
Fructose / 0 / 0 / 5.6476
Turanose / 4.33 / 0.722 / 0.349
Xylofuranose / 0.11 / 0.1 / 0.6
LC-MS
Metabolites / 0% NaCl / 9% NaCl / 18% NaCl
Citrate / √ / √ / √
isocitric acid / √ / √ / √
isopropyl malic acid / √ / √ / √
Glyoxylate / - / √ / √
Pyruvate / √ / √ / √
glyceraldehyde 3-phosphate / - / √ / √
Trehalose / √ / √ / √
disaccharides / √ / √ / √
D-hexose-phosphate / √ / √ / √
Glucosamine-1-P/ Glucose-1-phosphate / √ / √ / √
Glucosamine-6-P / √ / √ / √
Fructose-6-phosphate / √ / √ / √
Pantothenate / √ / √ / √
D-glucarate / √ / √ / √
NADPH / √ / √ / √
ddCMP / √ / √ / √
Deoxyribose-phosphate / √ / √ / √
ddCMP / √ / √ / √
ADP-D-glucose / √ / √ / √
cAMP/cyclic-AMP / √ / √ / √
Thymidine / √ / √ / √
Prephenate / √ / √ / √
dTDP / √ / √ / √
CDP / √ / √ / √
NADH / √ / √ / √
ADP-D-glucose / √ / √ / √
ADP-D-glucose / √ / √ / √
NADP+ / √ / √ / √
UTP / √ / √ / √
FMN / √ / √ / √
Glycerol-3-phosphate / - / √ / √
Xanthosine / √ / √ / √
dCMP / √ / √ / √
Reduced glutathione / √ / √ / √
Deoxyinosine / √ / √ / √
Deoxyadenosine / √ / √ / √
cGMP / √ / √ / √
UMP / √ / √ / √
AMP / √ / √ / √
S-adenosyl-L-methionine / √ / √ / √
Deoxyadenosine / √ / √ / √
Oxidized glutathione / √ / √ / √

STable 2 Comparison of the extracellular metabolites of Candida versatilis cultivated under free-salt, 9% and 18% salt environment, respectively.

OS-GC-MS-PCA1 / OS-GC-MS-PCA2
Metabolites / Relative peak area / Metabolites / Relative peak area
0% NaCl / 9% NaCl / 18% NaCl / 0% NaCl / 9% NaCl / 18% NaCl
3 - Methylthiopropanol / 0.069 / 0.116 / 0.173 / Ethanol / 0.033 / 0.045 / 0.029
2,3 - butanediol / 0.052 / 0.049 / 0.058 / Glycol / 0.021 / 0.052 / 0.039
Phenylethanol / 0.012 / 0.012 / 0.01 / Propylene glycol / 0.299 / 0.381 / 0.234
1,2 - butanediol / 0.008 / 0.009 / 0.019 / 2 - amino butyric acid / 0.016 / 0.093 / 0
Dihydroxyphenylethanol / 0.089 / 0.053 / 0.042 / Pyruvate / 0.012 / 0.031 / 0.010
Styrene-acrylic acid, beta-hydroxy--; / 0.072 / 0.081 / 0.029 / Niacin / 0.112 / 0.135 / 0.086
Formic acid / 0.111 / 0.14 / 0.352 / succinate / 0.808 / 1.344 / 0.3
DL-2-hydroxy-hexanoic acid / 0.031 / 0.009 / 0 / Malic acid; / 0.065 / 0.154 / 0.138
Benzoic acid / 0.081 / 0.042 / 0.024 / Glycerate / 0.047 / 0.071 / 0.068
3 - amino-isobutyric acid / 1.25 / 1.411 / 1.802 / 2 - hydroxy acid / 0.147 / 0.24 / 0.12
maleic acid / 0.084 / 0.117 / 0 / Palmitic acid / 0.568 / 0.885 / 0.725
Kojic acid / 0.034 / 0.036 / 0.018 / Stearic acid / 0.113 / 0.217 / 0.185
Glutaric acid / 0.006 / 0.007 / 0 / Glycerol fatty acid esters; / 0.144 / 0.283 / 0.198
Lactic acid / 0.468 / 0.736 / 1.707 / Glyceraldehyde / 0.056 / 0.095 / 0.066
Erucic acid; 3,5 - dimethoxy-4 - hydroxy cinnamic acid / 0.131 / 0.226 / 0.691 / Glutamic acid / 3.481 / 4.125 / 4.061
Oleic acid / 0.045 / 0.108 / 0.338 / Aspartate / 0.027 / 0.02 / 0.033
Glycolic acid / 0.03 / 0.075 / 0.085 / Isoleucine / 0.704 / 1.164 / 0.614
1,3 - dihydroxyacetone / 0.039 / 0.049 / 0.061 / N-acetyl-L-alanine / 0.236 / 0.462 / 0.17
Butylglycolate / 0.079 / 0.1 / 0.019 / Methionine / 0.3 / 0.904 / 0.661
Alanine / 0.01 / 0.008 / 0.006 / Galactopyranoside, methyl 4 - acetate / 0.042 / 0.075 / 0.053
Valine / 0.29 / 0.273 / 0.824 / 2 - (2 - furyl) glycolate / 0.071 / 0.162 / 0.107
Alanine / 1.338 / 1.742 / 2.431 / Ribose acid-1 ,4 - lactone / 0.019 / 0.037 / 0.0001
Norvaline / 0.872 / 1.424 / 1.378 / Mannopyranose, 6 - deoxy -; / 0.778 / 1.429 / 0.846
Threonine / 0.568 / 0.415 / 1.376 / Arabinofuranosyl / 0.028 / 0.157 / 0.081
Glycine / 0.069 / 0.139 / 0.315 / 5 - deoxy-D-ribose / 6.69 / 17.36 / 6.678
Cysteine​​; / 0 / 0.019 / 0.032 / Ribitol / 1.463 / 2.818 / 0.231
Pyroglutamate / 0.005 / 0.008 / 0.01 / Arabinose / 1.292 / 2.681 / 0.781
Serine / 0.543 / 0.403 / 1.946 / Mannose acid-1, 4 - lactone / 0.21 / 0.074 / 0.098
Ornithine; / 0.172 / 0.407 / 0.51 / Lactulose; 4-O-beta-D-galactopyranosyl-yl-alpha-D-fructose / 0.036 / 0.073 / 0.063
Tyrosine; / 1.046 / 1.272 / 1.834 / alpha-D-pyran-galactosidase, beta-D-furan-fructosyl / 40.77 / 16.71 / 15.81
Homocysteine​​; / 0.044 / 0.053 / 0.014 / Palatinose / 0.191 / 0.24 / 0.166
Asparagine; / 0.494 / 0.628 / 0.299 / Gluconate / 0.127 / 0.178 / 0.168
Homoserine / 0.035 / 0.013 / 0.0001 / Cadaverine / 0.187 / 0.016 / 0.103
Phenylalanine / 0.588 / 0.587 / 0.909
alpha-ketoglutarate / 0.009 / 0.013 / 0.041
Methyl-beta-D-ribofuranoside / 0.095 / 0.056 / 0.047
GDL / 0.045 / 0.026 / 0.017
Red pentose acid / 0.199 / 0.142 / 0.102
The D-glucoheptonic acid lactone / 0.102 / 0.173 / 0.0001
Acetyl-L-glutamine / 0.241 / 0.071 / 0
The glucoheptonate acid lactone / 0.181 / 0.216 / 0.049
L-gulonic acid-gamma-lactone / 0.04 / 0.08 / 0.087
Xylitol; / 29.352 / 32.357 / 6.027
Glycerophosphate / 1.01 / 0.321 / 0.041
L-(-) - Trehalose / 0.201 / 1.519 / 1.696
Maltose / 0.31 / 0.505 / 0.601
Turanose / 0.35 / 0.617 / 0.725
alpha-D-pyran-arabinose / 0.013 / 0.446 / 2.018
SPME-GC-MS-PCA1 / SPME-GC-MS-PCA2
Metabolites / Relative peak area / Metabolites / Relative peak area
0% NaCl / 9% NaCl / 18% NaCl / 0% NaCl / 9% NaCl / 18% NaCl
Phenylethanol / 30.22 / 43.55 / 30.25 / Neopentanol / 0.221 / 0.172 / 0
3,3 - methyl - 2 - pentanol / 8.083 / 7.426 / 12 / 3,3 - dimethyl-2 - butanol / 0.092 / 0.01 / 0
2 - hexyl -1 - n-octanol / 0.235 / 0.077 / 1.462 / 2,3 - butanediol / 0.043 / 0.022 / 0
2 - butyl octanol / 0.471 / 0.628 / 0.25 / Propionic acid / 0.593 / 0.323 / 0
2 - undecanol / 0.024 / 0.055 / 0.22 / Ethyl butyrate / 0.086 / 0.064 / 0
Isovalerate / 0.284 / 0.29 / 0.553 / :Methyl Lignocerate / 0.069 / 0.037 / 0
Ethyl octanoate / 0.095 / 0.028 / 1.715 / Iso-amyl acetate / 0.06 / 1.832 / 0.996
-1,2 Acetate - ethyl benzene / 8.415 / 15.37 / 10.47 / Ethyl crotonate / 0.047 / 0.024 / 0
17 Ethyl / 0.433 / 0.4 / 0.472 / Benzaldehyde / 0.426 / 0.3 / 0.209
Ethyl palmitate / 0.508 / 0.946 / 0 / Butylbenzene / 0.109 / 0.032 / 0.059
Ethyl laurate / 0.434 / 0.469 / 0 / 7 - 14 ene / 6.791 / 2.041 / 2.61
Ethyl stearate / 0.41 / 0.936 / 0.186 / Coumarone / 2.771 / 0.891 / 1.698
(Z) -9 - 16 carbon acid, 9 - 16 carbon alkenyl ester / 0.192 / 0.48 / 0 / Amyl benzene / 12.72 / 4.216 / 9.516
Titanium -1,2 - methoxy - 2 - phenethyl ester / 9.423 / 11.89 / 7.378 / Trans -2,4 - decadienal / 0.183 / 0.12 / 0.119
2 - Octanone / 7.144 / 2.074 / 10.55 / 2 - phenyl pentane / 2.138 / 0.608 / 1.581
2 - octene / 0.027 / 0.032 / 0.0001 / 7 - tetradecen / 1.521 / 0.626 / 0.728
Styrene / 0.085 / 0.132 / 0.059 / Hexadecanol / 1.609 / 0.664 / 1.233
Toluene / 0.292 / 0.252 / 1.082 / 6 - tridecenyl / 0.715 / 0.281 / 0.384
3 - propyl toluene / 0.204 / 0.036 / 0.148 / Nonadecane / 0.384 / 0.236 / 0.313
2 - ethyl-p-xylene / 0.155 / 0.026 / 0.144
Between Isopropyltoluene / 0.473 / 0.141 / 0.544
3 - amyl benzene / 0.218 / 0.225 / 0.585
2 - butene benzene / 1.215 / 0.307 / 1.234
4 - phenylbutyraldehyde / 0.27 / 0.138 / 0.357
1,4,8 - tetradecenyl, / 0.065 / 0.048 / 0.1
(1 - methyl-pentyl) - benzene / 0.295 / 0.11 / 0.251
3 - phenyl-furan- / 0.039 / 0.022 / 0.055
D-ribose / 0.693 / 0.631 / 0.55
DSE-GC-MS-PCA1
Metabolites / Relative peak area
0% NaCl / 9% NaCl / 18% NaCl
Phenylethanol / 75.88 / 78.94 / 33.98
Dihydroxyphenylethanol / 5.699 / 0.0001 / 12.28
1 - nonene-3 - ol / 0 / 1.109 / 2.075
Tryptophol / 1.685 / 0.754 / 0.351
Inositol / 0.507 / 0.812 / 0
N-butyric acid / 0.996 / 1.251 / 0
2 - methyl hexanoic acid / 0.238 / 0.234 / 0.209
3 - mercaptopropionic acid / 4.828 / 4.287 / 2.338
Kojic acid / 0 / 0.857 / 2.28
Phenylethyl acetate / 0.919 / 1.279 / 0.31
Methyl palmitate; / 0 / 0.302 / 2.11
5 - Methylfuran aldehyde / 0.129 / 1.006 / 1.348
5 - (hydroxymethyl) furfural / 0.854 / 0.119 / 2.934
4 - phenylbutyraldehyde / 1.231 / 1.632 / 0
2,5 - dimethylbenzaldehyde / 0 / 0.189 / 0.563
Phenol / 0.219 / 0.0001 / 21.7
2,6 - di-tert-butyl-p-cresol / 1.148 / 0.915 / 2.184
2,4 - di-tert-butyl-phenol / 1.606 / 1.208 / 4.058
3,5 - di-tert-butyl-4 - hydroxybenzaldehyde / 0.704 / 0.606 / 0.883
3,5 - dimethoxyphenol / 1.924 / 3.282 / 4.898
Hexadecane / 0.239 / 0.18 / 0.63
Fenugreek lactone; 3 - hydroxy-4,5 - dimethyl -2 (5H)-furanone / 0.216 / 0.121 / 0.617
OAC-LC
Metabolites / 0% NaCl / 9% NaCl / 18% NaCl
Oxalic acid / √ / √ / √
Formic acid / √ / √ / √
Malic acid / √ / √ / √
Lactic acid / √ / √ / √
Acetic acid / √ / √ / √
Fumaric acid / √ / √ / √
Citrate / √ / √ / √
LC-MS
Metabolites / 0% NaCl / 9% NaCl / 18% NaCl
isocitric acid / √ / √ / √
isopropyl malic acid / √ / √ / √
Trehalose / √ / √ / √
disaccharides / √ / √ / √
D-hexose-phosphate / √ / √ / √
Glucosamine-1-P/ Glucose-1-phosphate / √ / √ / √
Glucosamine-6-P / √ / √ / √
Fructose-6-phosphate / √ / √ / √
Pantothenate / √ / √ / √
Deoxyribose-phosphate / √ / √ / √
ADP-D-glucose / √ / √ / √
Thymidine / √ / √ / √
Prephenate / √ / √ / √
ADP-D-glucose / √ / √ / √
isopropyl malic acid / √ / √ / √
FMN / √ / √ / √
Xanthosine / √ / √ / √
Reduced glutathione / √ / √ / √
Deoxyinosine / √ / √ / √
Deoxyadenosine / √ / √ / √
S-adenosyl-L-methionine / √ / √ / √
Deoxyadenosine / √ / √ / √
Oxidized glutathione / √ / √ / √
UMP / √ / √ / √
AMP / √ / √ / √
Deoxyadenosine / √ / √ / √
Oxidized glutathione / √ / √ / √

STable 3 Comparison of the whole metabolites of Candida versatilis cultivated under free-salt, 9% and18% salt environment, respectively.

OS-GC-MS-PCA1 / OS-GC-MS-PCA2
Metabolites / Relative peak area / Metabolites / Relative peak area
0% NaCl / 9% NaCl / 18% NaCl / 0% NaCl / 9% NaCl / 18% NaCl
Glycol / 0.037 / 0.153 / 0.187 / 2 - methylbutylaldehyde / 0.051 / 0.025 / 0.032
2,3 - butanediol / 0.583 / 0.741 / 0 / 1,2 - butanediol / 7.597 / 0.039 / 3.793
Propylene glycol / 2.778 / 2.409 / 4.648 / 1,4 - butanediol / 0.059 / 0.197 / 0.072
Butanol / 0 / 0.041 / 0.167 / 1,2,3 - Butanetriol / 0.271 / 0.061 / 0
Phenylethanol / 0.098 / 0.133 / 1.349 / Isopropanol / 0.27 / 0.0001 / 0.025
Dihydroxyphenylethanol / 0.064 / 0.244 / 0.657 / 2 - pentanol / 0.442 / 0.005 / 0.07
glycerol / 3.667 / 1.513 / 0 / 2 - amino butyric acid / 0.647 / 0.085 / 0.274
Undecenol / 0.257 / 0.091 / 2.728 / Isovalerate / 0.013 / 0.084 / 0
Diethylene glycol / 0.25 / 0.152 / 0 / Lactic acid / 0.629 / 1.252 / 0.335
Acrylic acid / 0.0001 / 0.0001 / 0.031 / Succinic acid / 0 / 1.023 / 0.255
Isobutyric acid; 2 - methyl propionate / 0.019 / 0.013 / 0 / Oxalic acid / 0 / 0.044 / 0.0001
Glycerate / 0.098 / 0.055 / 0.264 / Citric acid / 0.047 / 0.734 / 0.017
Phthalate / 0.011 / 0.029 / 0.509 / L-malic acid / 0 / 0.114 / 0.029
Benzoic acid / 0.143 / 0.005 / 0.48 / Malonic / 0 / 0.111 / 0.013
Kojic acid / 0.038 / 0.046 / 0 / Ethyl acetate / 0.017 / 0.212 / 0.18
DL-pantothenyl lactone / 0.018 / 0.007 / 0 / Phenylketonuria / 0.46 / 0.031 / 0.229
L-valine; / 0 / 0.023 / 1.358 / 1,3 - dihydroxyacetone / 0 / 0.13 / 0.015
Arabinose / 3.617 / 8.143 / 0.109 / Glucopyranosyl-1 ,2,3,4 - 3-O-methyl - / 0.198 / 0.04 / 0.052
D-seven grape ketone and 1,4 - lactone / 0.166 / 0.204 / 0.093 / Red pentose acid / 0.073 / 0.486 / 0.042
D-glucuronic acid; / 0.156 / 2.15 / 3.247 / GDL / 0.035 / 0.184 / 0.011
Ribulose / 0.118 / 0.066 / 0.046 / The D-glucoheptonic acid lactone / 0.085 / 0.522 / 0.0001
L-(-) - Trehalose / 0.243 / 1.011 / 17.72 / 2 - (2 - furyl) glycolate / 0.053 / 0.662 / 0
Gluconate / 0.109 / 0.216 / 10.98 / Xylitol / 0.97 / 25.24 / 0.623
Xylulose / 0.126 / 0.175 / 3.585 / L-mannose acid-1 ,4 - lactone / / 1.448 / 0.133 / 0.043
alpha-D-pyran-arabinose / 0.043 / 0.301 / 5.778 / D-arabitol / 2.685 / 0.151 / 0.089
alpha-D-galactopyranoside, alpha-D-galactopyranosyl / 0.313 / 0.626 / 0.065 / D-ribose / 0.142 / 0.688 / 0.306
D-(+) - ribose acid 1, 4 - lactone / 2.795 / 3.749 / 0 / Pentitol / 17.6 / 0.228 / 0.41
Palatinose / 0.267 / 0.094 / 0 / Xylose / 12.84 / 0.622 / 1.673
Maltose / 0.256 / 0.146 / 0.042 / L-gulonic acid-gamma-lactone / 10.6 / 2.169 / 0.496
alpha-D-galactosidase, / 10.19 / 29.6 / 0.422 / alpha-D-pyran-galactosidase, beta-D-furan-fructosyl / 7.737 / 0.037 / 0.0001
Fructose; / 0.022 / 0.032 / 6.476 / Turanose / 4.33 / 0.722 / 0.349
D-glucopyranose; D-galactose / 0.315 / 0.629 / 3.06 / DL-ribopyranosylamine / 0.061 / 0.175 / 0.065
Lactose / 0.059 / 0.078 / 0.0001 / D-pentose acid -1,3 - deoxy -; / 0.069 / 0.281 / 0.06
5 - deoxy-D-ribose / 0.598 / 0.276 / 0 / Galactopyranoside, methyl 4 - acetate / 0.015 / 0.594 / 0.49
2 - deoxy-D-ribose / 0.047 / 0.026 / 0 / alpha-D-galactosidase, / 2.783 / 8.957 / 0.313
2,4 - dihydroxybenzoic acid / 0.021 / 0.016 / 6.951 / D-glucose anhydrous; / 0.046 / 0.527 / 0.032
Isomaltose / 0.109 / 0.06 / 0.576
alpha-Methylglucoside; / 0.085 / 0.185 / 7.896
SPME-GC-MS-PCA1 / SPME-GC-MS-PCA2
Metabolites / Relative peak area / Metabolites / Relative peak area
0% NaCl / 9% NaCl / 18% NaCl / 0% NaCl / 9% NaCl / 18% NaCl
2,3 - butanediol / 0.11 / 0.368 / 0.0001 / Hexadecanol / 1.653 / 0.579 / 0.998
Neopentanol / 0.122 / 0.226 / 0 / 2 - hexyl -1 - n-octanol / 0.165 / 0.084 / 0.109
2 - undecanol / 0.107 / 0.072 / 0.305 / 2 - butyl octanol / 0.45 / 2.396 / 0.912
3,3 - methyl - 2 - pentanol / 9.055 / 8.809 / 5.94 / Ethyl crotonate / 0.098 / 0.039 / 0
Phenylethanol / 32.92 / 45.92 / 22.68 / :Methyl Lignocerate / 0.069 / 0.019 / 0
Propionic acid / 0.571 / 0.363 / 0 / Ethyl palmitate / 2.246 / 0.57 / 0
Ethyl butyrate / 0.158 / 0.172 / 0 / Ethyl laurate / 0.697 / 0.17 / 0
Ethyl octanoate / 0.093 / 0.058 / 0.132 / Ethyl stearate / 0.245 / 0.149 / 0.175
Titanium, 2 - phenethyl ester / 8.515 / 15.07 / 7.542 / (Z) -9 - 16 carbon acid, 9 - 16 carbon alkenyl ester / 0.056 / 0.016 / 0
Titanium -2 - methoxy - 2 - phenethyl ester / 8.439 / 10.43 / 5.632 / Ethyl heptadecanoate / 0.945 / 0.238 / 0.369
The hexanoate -2 - ethyl benzene / 0.062 / 0.163 / 0 / Ethyl acetate / 0 / 0.278 / 0.208
Iso-amyl acetate / 0.038 / 0.063 / 0 / Toluene / 7.65 / 1.762 / 0.593
Benzaldehyde / 0.279 / 0.297 / 0.191 / Benzene, (1 - methylpentyl) - / 0.208 / 0.239 / 0.219
Coumarone / 0.844 / 0.373 / 7.214 / Butylbenzene / 0.217 / 0.047 / 0.075
2 - Octanone / 4.309 / 3.138 / 18.14 / 7 - tetradecen / 1.776 / 0.499 / 0.706
3 - phenyl-furan- / 0.112 / 0.091 / 0.128 / 6 - tridecenyl / 0.757 / 0.021 / 0.455
2 - methylheptanoic aldehyde / 0.514 / 0.043 / 1.196 / Nonadecane / 0.512 / 0.121 / 0.184
3 - propyl toluene / 0.091 / 0.031 / 0.231
2 - ethyl-p-xylene / 0.058 / 0.027 / 0.133
Between Isopropyltoluene / 0.229 / 0.122 / 0.608
Trans -2,4 - decadienal / 0.234 / 0.046 / 0.237
2 - octene / 0.056 / 0.0001 / 0.145
Styrene / 0.197 / 0.197 / 0.041
7 - 14 ene / 2.582 / 0.842 / 5.503
3 - amyl benzene / 0.469 / 0.22 / 0.564
2 - butene benzene / 0.559 / 0.252 / 1.456
Amyl benzene / 10.29 / 4.128 / 14.02
2 - phenyl pentane / 1.164 / 0.496 / 1.948
1,4,8 - tetradecenyl, / 0.081 / 0.565 / 0
Trans-5 - decene / 0 / 0.081 / 0.323
DSE-GC-MS-PCA1 / DSE-GC-MS-PCA2
Metabolites / Relative peak area / Metabolites / Relative peak area
0% NaCl / 9% NaCl / 18% NaCl / 0% NaCl / 9% NaCl / 18% NaCl
Inositol / 1.291 / 0.991 / 3.118 / Tryptophol / 2.423 / 1.069 / 1.484
Dihydroxyphenylethanol / 0.167 / 0.0001 / 5.957 / 2 - methyl hexanoic acid / 0.335 / 0.248 / 0.263
1 - nonene-3 - ol / 0 / 1.065 / 2.577 / 3 - mercaptopropionic acid / 2.189 / 4.528 / 2.24
N-butyric acid / 0.866 / 1.069 / 0 / Linoleic acid / 0.414 / 0.111 / 0
Isovalerate / 0.335 / 0.283 / 0.427 / Phenol / 1.346 / 0.315 / 0.387
Phenylethanol / 77.66 / 76.852 / 35.03 / 3 - tert-butylphenol / 0 / 0.294 / 0.35
Kojic acid / 0.11 / 1.543 / 4.762 / D-ribose / 0.949 / 0.681 / 0.98
Formic acid phenethyl ester / 0.575 / 1.815 / 0
2 - hydroxy ethyl valerate / 0.503 / 0.232 / 0
Phenylethyl acetate / 0.127 / 0.346 / 0
Fenugreek lactone; 3 - hydroxy-4,5 - dimethyl -2 (5H)-furanone / 0.193 / 0.226 / 0.91
4 - phenylbutyraldehyde / 0.771 / 1.772 / 0
2,5 - dimethylbenzaldehyde / 0.374 / 0.218 / 0.501
5 - Methylfuran aldehyde / 0.624 / 0.781 / 3.145
2,6 - di-tert-butyl-p-cresol / 1.189 / 0.93 / 2.807
2,4 - di-tert-butyl-phenol / 2.08 / 1.154 / 5.406
3,5 - di-tert-butyl-4 - hydroxybenzaldehyde / 0.91 / 0.42 / 2.87
3,5 - dimethoxyphenol / 4.01 / 2.777 / 10.85
Hexadecane / 0.229 / 0.197 / 0.477
OAC-LC
Metabolites / 0% NaCl / 9% NaCl / 18% NaCl
Oxalic acid / √ / √ / √
Formic acid / √ / √ / √
Malic acid / √ / √ / √
Lactic acid / √ / √ / √
Acetic acid / √ / √ / √
Citric acid / √ / √ / √
Fumarate / √ / √ / √
glyceraldehyde 3-phosphate / √ / √ / √
LC-MS
Metabolites / 0% NaCl / 9% NaCl / 18% NaCl
D-glucarate / √ / √ / √
Trehalose / √ / √ / √
disaccharides / √ / √ / √
D-hexose-phosphate / √ / √ / √
Glucosamine-1-P/ Glucose-1-phosphate / √ / √ / √
Glucosamine-6-P / √ / √ / √
Fructose-6-phosphate / √ / √ / √
Pantothenate / √ / √ / √
D-hexose-phosphate / √ / √ / √
NADPH / √ / √ / √
ddCMP / √ / √ / √
Deoxyribose-phosphate / √ / √ / √
ddCMP / √ / √ / √
ADP-D-glucose / √ / √ / √
cAMP/cyclic-AMP / √ / √ / √
Thymidine / √ / √ / √
Prephenate / √ / √ / √
dTDP / √ / √ / √
CDP / √ / √ / √
NADH / √ / √ / √
ADP-D-glucose / √ / √ / √
isopropyl malic acid / √ / √ / √
ADP-D-glucose / √ / √ / √
NADP+ / √ / √ / √
NADPH / √ / √ / √
UTP / √ / √ / √
FMN / √ / √ / √
Glycerol-3-phosphate / √ / √ / √
Xanthosine / √ / √ / √
dCMP / √ / √ / √
Reduced glutathione / √ / √ / √
Deoxyinosine / √ / √ / √
Deoxyadenosine / √ / √ / √
cGMP / √ / √ / √
UMP / √ / √ / √
AMP / √ / √ / √
S-adenosyl-L-methionine / √ / √ / √
Deoxyadenosine / √ / √ / √
Oxidized glutathione / √ / √ / √
Deoxyadenosine / √ / √ / √
Citrate / √ / √ / √
isocitric acid / √ / √ / √

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