ADVANCED BIOCHEMISTRY
Lecture Schedule
Chemistry A8005 Spring 2010
Room MR1026
Mon, Wed 9:30-10:45 Professor Steinberg
======
Date Topic Text
Feb 1 Mon Photosynthesis light reaction Lehninger pp. 742-758
3 Wed Photosynthetic carbohydrate Lehninger pp. 773-786
biosynthesis
8 Mon other carbohydrates, polyaccharides Lehninger pp. 786-788,791-796
10 Wed Immune & complement systems Lehninger pp. 170-174, handout
17 Wed The blood clotting system Handout
18 Thurs biological products of amino acids Lehninger pp. 873-882
22 Mon Exam I
24 Wed Biosignaling Lehninger pp. 419-449
Mar 1 Mon Biosignaling Lehninger “
3 Wed Biosignaling Lehninger pp. 457-468
8 Mon Regulation of Glycogen metabolism Lehninger pp. 584-592, 604-608
10 Wed Hormonal regulation of metabolism Lehninger pp. 583-592, 902-930
15 Mon Hormonal regulation of metabolism Lehninger pp. 923-940
17 Wed Exam II
22 Mon Neurobiochemistry Lehninger pp 449-455, Handout
24 Wed Neurobiochemistry Handout
-spring break March 29- April 5
Apr 7 Wed Phage lambda – lytic cycle Handout
12 Mon Phage lambda – lysogenic cycle Handout
14 Wed Recombinant DNA technology Handout
19 Mon Recombinant DNA technology Handout
21 Wed Exam III
26 Mon Regulation of gene expression Lehninger pp 1136-1144
28 Wed Mitochondrial dysfunction Handout
in cancer
May 3 Mon Oncogenes & retroviruses Handout
5 Mon Oncogenes, signaling pathways Handout, Lehninger pp.469-479
and cell cycle regulation
10 Mon Genetic disease Handout
12 Wed Genetic disease Handout
17 Mon Exam IV
Text: LehningerPrinciples of Biochemistry: Nelson & Cox, 2005,
5th Ed. W.H. Freeman & Co., NY.
Office hours: Mon 1-3 PM, Wed 2-4 PM. Office: MR629; Tel: (212) 650-8560; Fax: (212) 650-6092; email: .
Grading: based on 4 lecture exams. Students will be given the option to take a cumulative final exam. The final exam can be used to replace one regular exam grade or as a makeup for ONE missed exam
Catalog Description:
A8005: Biochemistry II. Molecular basis of enzyme action, membranes (transport and signal transduction), protein structure, signal transduction, virology, bioinformatics, genomics, proteomics, molecular basis of replication, transcription of genetic information, immunology. Prereq: a one semester undergraduate biochemistry course.
Prerequisites:Chem. 45900
Hours/Credits:3 hours per week, 3 credits
Textbook:Principles of Biochemistry, 5th ed., by David L. Nelson, Michael M. Cox and Albert Lehninger ISBN 978-1429224161, yr. 2005
Course objectives:
The first half of the course covers advanced topics in biochemistry that build upon the basic principles of biochemistry covered in Chemistry 45900 (Principles of Biochemistry) including photosynthesis, synthesis of complex carbohydrates, elements of the immune system, signaling pathways/hormone action that regulate metabolic reactions, biochemical principles of neuron function.
The second half of the course explores contemporary areas in modern biochemistry with a focus on gene function and expression in eukaryotic and in prokaryotic systems as exemplified by bacteriophage lambda. Students will also learn some of the basic concepts and practical applications of biotechnology including gene cloning and expression in engineered vector systems, artificial chromosome construction, site directed mutagenesis, and approaches to gene therapy. Southern blotting/RFLP analysis using pulsed field gel electrophoresis and chromosome walking will be discussed in the context of markers of genetic disease. This part of the course will also examine the biochemical bases of various disease states resulting from alterations in metabolic processes with a focus on mitochondrial-based diseases. Several lectures will be devoted to retroviral oncogenes and tumor suppressors and their relationship to signaling pathways involved in the regulation of cell growth and cancer
After completing this course, students should:
- know the basic steps and important intermediates in the light and dark reactions of
photosynthesis.
- know the basic steps and important intermediates in the synthesis of complex
carbohydrates and extracellular matrix polysaccharides.
- be able to distinguish between the humoral and cellular immune systems and know the
basic components and the function of the components of each system.
- be able to detail the main steps and intermediates in the pathways by which hormones
- regulate metabolism via glycolysis and the Kreb’s cycle and carbohydrate transport.
- be able to outline the basic steps and intermediates of the major signal transduction pathways (MAP kinase, JAK-STAT, PKA, PPAR) and the specific biological processes regulated by the pathways covered in class.
- be able to outline the steps in the biotechnological approaches to genetic disease
including the genetic basis of the cancers discussed in class.
- be able to describe the functions of the elements of lambda bacteriophage in the
lytic cycle or lysogeny
- be able to describe the basic steps in the techniques of biotechnology described in
class. Students should be able to provide examples of how these techniques are
applied to modern biomedicine, gene therapy and genetic engineering
- know the structure and function of the basic ion channel types involved in neural
transmission and the experimental techniques used to analyze how they function in
motor control, memory and sensory input.
- be able to outline the steps and critical enzymes involved in the generation of
reactive oxygen species (ROS) in mitochondria and how mitochondrial-derived ROS
activate particular signaling pathways.
- be able to describe the processes by which retroviral oncogenes are transduced and
subsequently activated in the host. Students should know how the human homologs of
the oncogenes covered in class function in cell cycle control and cancer.
Attendance policy – The lectures distill the critical elements and concepts from the source materials (textbook and handouts) that will be presented in the exams. Also, there may be some important information presented in lecture that is not covered in the source materials. For these reasons it is necessary to attend lectures in order to gain the most from the course and to maximize performance on exams.
Statement on Academic Integrity - The CCNY policy on academic integrity will be followed in this course. The policy statement can be found by logging onto the CCNY website and then following the links: Current Students → Academic Services/Tutoring → Policy on Academic Integrity. All students must read the details regarding plagiarism and cheating in order to be familiar with the rules of the college. Cases in which there are violations of academic integrity will be prosecuted according to these rules. The Policy on Academic Integrity can also be found in the Undergraduate Bulletin 2009-2010.