Agreement No. F12FTSJPJTXXXX
Information of Draft map for fungi samples
*Requirements for Sample Quality*
Sample requirement:
l Requirements for DNA sample
1) Sample condition: DNA samples;
2) Sample quantity (for a single library preparation): for short-insert libraries, ≥ 6 μg; for 2 Kb~6 Kb large-insert libraries, ≥ 40 μg; for PCR-free libraries constructed for the genome with unnormal GC content (<35% or >65%), ≥20 μg; the total quantity is determined by the experimental strategy of the type and number of libraries to be constructed;
3) Sample concentration: for short-insert libraries, ≥ 30 ng/μl; for 2 Kb~6 Kb large-insert libraries, ≥ 133 ng/μl
4) Sample purity: A260/280=1.8~2.0;
5) Notice:
1) The final concentration is determined by QUBIT result after the samples are received. Provider determines that Customer failed to provide sufficient amounts of sample for analysis or that the sample fails Provider’s then-current QC Criteria, Provider shall notify the Customer and Customer shall replace the sample within 20 days and Customer should pay extra fee for sample testing. If Customer still insists on getting the “unqualified samples” sequenced, Provider would sequence the “unqualified samples” without any responsibilities. Provider could provide TA cloning test for the possible contamination of samples, the payment in accordance with USD500/sample. But the TA cloning test is free if sample contamination occurs in Provider. Customer should describe the number of the fungi chromosome and notify whether plasmid, chondriosome, chloroplast and other extraneous DNA exist in the fungi sample. Sequencing just refers to the fungi genome, not containing extraneous DNA in vivo. If these foreign DNA present in the samples, they will probably be sequenced and then assembled to contigs. If Customer Would likes to sequence these extraneous DNA, please contract separately.
2) A copy of the DNA sample will be saved if the DNA sample is more than required for experiment.
3) The rest DNA sample won’t be saved after project is finished.
Please attach the Gel electrophoresis analysis result to make sure the samples are not degraded or contaminated. And please provide the type of Spectrophotometers or Fluorospectrometers used during sample preparation.
Samples classified as level A are qualified and sufficient for library construction for more than twice, while those of level B are sufficient for library construction for once. Sample preparation following the standard of level A is recommended for guaranteeing a normal progress of the project. If samples fail to meet the standard even of level B, and for some reason, no more samples could be obtained, please contact BGI Project Coordinator and Project management before sample shipment for consulting.
* Notice of Filling Forms *
1. Please provide analysis results measured by using Gel Electrophoresis graph, NanoDropTM and Agilent Bioanalyzer or other methods of one sort or multi-forms. When using Gel Electrophoresis graph and Agilent Bioanalyzer to test gel or other information, the client should indicate the band size of marker in the gel graph.
2. In order to help experiment staffs to test and deal with samples quickly, please fill in the following carefully. Any questions please contact BGI Project Coordinator or highlight them. Do not leave blank in the form (Those columns marked with an asterisk mark (*) are required ones.)
3. If you do denovo analysis, please contact the project leader to confirm the library type of the sample.
4. After BGI receiving samples, DNA or Library stored at -20 ℃ refrigerator, RNA or tissue stored at -80 ℃ refrigerator. The special conditions for sample storage, clients please describe the correct storage temperature in the information sheet. Antibodies, Primers, and Probes sent along with the samples also need to specify the corresponding information of stored temperature.
5. According to the experimental conditions, BGIwould possibly dilutethe original sample beforedetection if the sample volume is too small (less than 15 μL). If the sample is notpreserved in the 1.5 mL EP tube, BGI would change tube firstly.
6. If the sample or analysis type could not meet the requirement of routine DNA sample, please contact BGI Project Coordinator and marked with an asterisk mark (*) in Customized type. (Notice: The customized type projects have to be preapproved by customized manager before the contract signed). The Customized analysis type including: (1) FFPE Re-sequencing; (2) FFPE Exome sequencing; (3) MBD sequencing; (4) Truseq Exome sequencing; (5) cDNA amplification sample Transcriptome.
7. Shipping information:
Consignee’s Name / BGI JAPANConsignee’s Address / 〒650-0047日本兵庫県神戸市中央区港島南町1-5-2神戸キメックセンタービル8F
Contact No / 078-599-6108
Email /
Please be reminded that all samples have to send to the following address:〒650-0047日本兵庫県神戸市中央区港島南町1-5-2神戸キメックセンタービル8F. Please do not send the shipments to other address (such as airport), which may cause loss of shipment/ delayed delivery.
After the samples have been picked up, please send the name of courier, tracking no. and sample information sheet to BGI JAPAN () immediately. With the information, BGI will help to track for the shipping status andfacilitatethe importation progress.
* Sample information sheet *
Note:Filling inthis formwith detailedandaccurateinformation is the first step for us to serveyou better,thankyouforyourcooperation! (*meanscompulsoryinformation)Transport Information* / Courier companies: Tracking no.:
Contact Person: E-mail: City:
Transport Condition: □ Dry ice □ Ice bag □ Room temperature □ Others:
Contract Number* / Project Name*
Name of Client* / Company/Institute*
BGI Project Coordinator*
NGS Platform* / □ 1. HiSeq 2000 □ 2. SOLiD4.0 □ 3. 454 GS FLX+ □ 4. Others:
Project Type* / □ 1. Standardized project □ 2. Customized project (Customized type: )
Analysis type*
□ 1.De Novo Sequencing ( □ ≤800bp insert normal library, □ ≤800bp insert PCR free library, □ 2K insert library, □ 5-6K insert library, □ 10K insert library, □ 20/40K insert library )
□ 2.Whole Genome Re-sequencing ( □ ≤800bp insert normal library, □ ≤800bp insert PCR free library, )
□ 3.MeDIP Sequencing or Bisulfite Sequencing
□ 4.ChIP Sequencing
□ 5.RAD Sequencing
□ 6.Exome Sequencing
□ 7.Genotyping
□ 8.Metagenomic Sequencing
□ 9.Others:
Sample Information：
Ø Type*: □ Genomic DNA □ PCR products □ Broken into short fragments
□ ChIP-Seq DNA samples □ Others:
Ø Condition*: □ Dissolved in water □ Dissolved in TE Buffer □ Dry powder
□ Dissolved in pure ethanol □ Others :
Ø The main ingredient of the sample*: (e.g. 10% water, 75% pure ethanol, 15% DNA)
Ø Treat by RNase: □ Yes □ No
Sample Name* / Species* / No. of
Tubes* / Concentration (ng/μL) / Volume (μL) / Total
Quantity (μg) / Fragment
Size / OD260/280 / OD260/230 / Remarks
Please indicate the sample detecting methods: which samples need to be combined, and which samples need separate detection;
Sample Name must consistent with the sample tube mark.
1 / 3