Name: Date:1

Lab Partner: Period:

Anatomy & Physiology

The Rate of Paperase

Objectives:

To learn about enzyme reactions using imaginary enzymes (your hands, a.k.a. sugar paperase) and molecules (paperose)

To determine the rate of reaction using enzymes

Introduction:

In this lab, your hands are the enzyme paperase. The function of this enzyme is to split the sugar paperose into subunit A and subunit B. By reaching into the cup and taking out one paperose molecule at a time, you will split this molecule by ripping the paper molecule down the middle one at a time. After every molecule that is ripped, it will be placed back into the cup. See procedure.

Each group will have no more than 3 members. EVERY student turns in their OWN report.

Materials:

Name: Date:1

Lab Partner: Period:

Paperose Molecules

Scissors (if not cut)

Container

Graph Paper

Calculator

Clock/Timer

Pen

Lab

Name: Date:1

Lab Partner: Period:

Procedure: READ ALL INSTRUCTIONS BEFORE BEGINNING!!

  1. Once you have established your group, be sure to have 50 paperose molecules in your cup. (There should be 2 subunits to each molecule.) You & your partner will need to cut out molecules!

A|B

  1. One member will do the following:
  2. When the timer is ready, remove one paperose molecule from the cup and rip it down the middle. Only rip ONE MOLECULE AT A TIME.
  3. Place both pieces back into the cup.
  4. Take out another molecule and rip that one. Place both pieces back into the cup.
  5. Continue this procedure for 10 seconds.
  6. Another member acts as the timer while another acts as the recorder (unless there are only 2 members to each group; if this is the case, the second member times and records). Every member within the group should have the same data/results.
  7. Repeat process (in step 2) for 30, 60, 120, and 180 seconds.
  8. Each student should graph their results INDIVIDUALLY.
  9. Determine the rate of reaction for the following times: 10, 30, 60, 120, and 180 seconds. Record this data in Table 2.
  10. The instructor will gather the individual group data for you to find a class average. Record this information in table 3 AND each student should graph these results.
  11. Answer the conclusion questions (in dark blue or black in AND in complete sentences).

Results:

Table 1: Time Trial Results

Time (seconds) / 10 / 30 / 60 / 120 / 180
Number of Paperose Molecules Ripped

Graph Table 1 Data.

Table 2: Rate of Reaction for Each Time Trial

Time (seconds) / 10 / 30 / 60 / 120 / 180
Rate of Reaction in Molecules per Second

Rate of Reaction = # of molecules ripped ÷ time

Table 3: Class Average for Time Trial Results

Time (seconds) / 10 / 30 / 60 / 120 / 180
Number of Paperose Molecules Ripped

Graph Table 3 Data.

Table 4: Class Rate of Reaction for Each Time Trial

Time (seconds) / 10 / 30 / 60 / 120 / 180
Rate of Reaction in Molecules per Second

Rate of Reaction = # of molecules ripped ÷ time

Conclusion Questions on the next page 

Conclusion Questions:

1. If you were allowed to continue this lab and rip paperose molecules for 240 and 300 seconds, what would you expect would have happened to the rate of reaction? WHY?

2. Compare your two graphs. Are they similar or not? What accounts for this and why? Explain your results compared to the class results.

3. If you took paperose molecules and dispersed them around the room:

Would the molecules be more or less concentrated?

Would there be more or less reactions (ripped paperose molecules)? Explain your response.

4. How does the concentration of the substrate relate to the rate of reaction? Think about this reaction. What was the substrate in this lab?

5. Based on this lab, what can you conclude about the length of time in relation to the rate of reaction? What was the limiting factor in this lab?

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