RODENTENDPOINT PCR/RT-PCR FORM (D-1-M)

Please print and complete this form and forward (via e-mail or fax to MDS/RRDA. We will contact you for a detailed quotation.

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Services Requested:

Mouse Panels:

18 Tests Panel

  1. Mycoplasma pulmonis
  2. Sendai Virus
  3. Mouse Hepatitis Virus
  4. Pneumonia Virus of Mice
  5. Minute Virus of Mice
  6. Mouse Parvovirus
  7. Theiler's GD-VII
  8. Reo-3 Virus
  9. Mouse rotavirus
  10. Ectromelia (Poxvirus)
  11. Lymphocytic Choriomeningitis Virus
  12. Polyoma virus
  13. K Virus
  14. Mouse Adenovirus
  15. Mouse cytomegalovirus
  16. Lactodehydrogenase Virus
  17. Choice of Mouse Thymic Virus or Mouse Parvovirus (MMV)
  18. Choice of Hantaan virus or other Mycoplasma spp. *

15 Tests Panel

  1. Mycoplasma pulmonis
  2. Sendai Virus
  3. Mouse Hepatitis Virus
  4. Pneumonia Virus of Mice
  5. Minute Virus of Mice
  6. Mouse Parvovirus
  7. Theiler's GD-VII
  8. Reo-3 Virus
  9. Mouse rotavirus
  10. Ectromelia (Poxvirus)
  11. Lymphocytic Choriomeningitis Virus
  12. Polyoma virus
  13. K Virus
  14. Mouse Adenovirus
  15. Mouse cytomegalovirus

13 Tests Panel

  1. Mycoplasma pulmonis
  2. Sendai Virus
  3. Mouse Hepatitis Virus
  4. Pneumonia Virus of Mice
  5. Minute Virus of Mice
  6. Mouse Parvovirus
  7. Theiler's GD-VII
  8. Reo-3 Virus
  9. Mouse rotavirus
  10. Ectromelia (Poxvirus)
  11. Lymphocytic Choriomeningitis Virus
  12. Polyoma virus
  13. K Virus

Rat Panels:

15 Tests Panel

  1. Mycoplasma spp.
  2. Pneumonia virus of mice
  3. Kilham’s rat virus
  4. Toolan’s H1 virus
  5. Rat Parvovirus
  6. Lymphocytic Choriomeningitis Virus
  7. Rat cytomegalovirus
  8. Sendai Virus
  9. Rat coronavirus
  10. Sialodacryoadenitis virus
  11. Rat minute virus
  12. Seoul virus
  13. Mouse Adenovirus
  14. Reovirus 3
  15. Theiler’s murine encephalomyelitis virus

11 Tests Panel

  1. Mycoplasma spp.
  2. Pneumonia virus of mice
  3. Kilham’s rat virus
  4. Toolan’s H1 virus
  5. Rat Parvovirus
  6. Lymphocytic Choriomeningitis Virus
  7. Rat cytomegalovirus
  8. Sendai Virus
  9. Rat coronavirus
  10. Sialodacryoadenitis virus
  11. Rat minute virus

Hamster Panel:

11 Tests Panel

  1. Mycoplasma spp.
  2. Pneumonia virus of mice
  3. Kilham’s rat virus
  4. Toolan’s H1 virus
  5. Hamster Parvovirus
  6. Lymphocytic Choriomeningitis Virus
  7. Mice minute virus
  8. Theiler’s murine encephalomyelitis virus
  9. Sendai virus
  10. Reovirus 3
  11. Hantaan virus

Other animal pathogens

Rotavirus, equineRotavirus, felineRotavirus, canine

Rotavirus, bovineRotavirus, porcineEquine influenza virus

Equine protozoal myeloencephalitis (Sarcocystis neurona)

Samples (For multiple samples, please complete Form D-3.)

What type of sample will be submitted? (see recommendations page 3)

Sample source:humanratmouseother

Cell culture (flask size; number of flasks ; passage # )

Cell pellet (number of cells ; lot # )

Tissue (type ; amount )

DNA (concentration ; volume ; total amount ng)

RNA (concentration ; volume ; total amount ng)

Lysis buffer (if applicable)

Special components of buffer (if applicable)

Recommended Sample Size for Endpoint PCR

Sample

/ Amount
Cultured cells / 5 x 10^6
Liver / 25 mg (~2mm3)
Brain / 25 mg
Lung / 25 mg
Heart/muscle / 25 mg
Kidney / 25 mg
Spleen / 10 mg
Plant leaf / 100 mg
Mouse tail / 0.8-1.2 cm
Blood / 1 ml
Buffy coat / 50 ul
DNA / 50 ng/reaction
RNA / 100 ng RNA/reaction
Other / Call

Recommended Shipping Conditions

Plasmid DNA / Liquid = A, W, D; pellet A
PCR products / Liquid = A, W, D; pellet A
Primers / Liquid = A, W, D; pellet A
cDNA or genomic libraries / With DMSO = D; without DMSO = W
RNA / D
Bacterial colonies / A, W
Stab culture / A
Cell banks / D
Glycerol stocks / D
Tissues / D
Blood / W

A = ambient temperature W = wet ice D = dry ice