DRAFT
MANAGEMENT OF WOSR REFERENCE COLLECTIONS
REPORT OF FIRST PROJECT MEETING, 27 APRIL 2005, NAIB, CAMBRIDGE, UK
ATTENDENCE:
Dr Robert J CookeNIAB, UKProject co-ordinator
Francoise BlouetGEVES, F
Cecille CollonnierGEVES, F
Ann BernolleGEVES, F
Beate RueckerBSA, D
Gerhard DenekenDIAS, DK
Louise Bach JensenDIAS, DK
Dr Carol NorrisNIAB, UK
Dr David LeeNIAB, UK
Huw JonesNIAB, UK
Anne WeitzCPVO
Dr John AustinDefra, UK
1)Dr Cooke welcomed the partners and participants and introduced the project, out-lining the milestones and deadlines that need to be met.
2)The objectives of this meeting were to agree which molecular markers should be used, decide which varieties would be included and decide any issues related to the data (both morphological and molecular) that would be generated during the project.
3)Molecular markers: Prior to the meeting, NIAB had circulated 10 DNA samples to F and DK, along with the sequences of 29 SSRs and a draft analytical protocol. Participants from these 3 countries discussed these results, and agreed that there were 14 markers that could be analysed and scored reliably. In addition, there were 4 markers that GEVES were going to check again, 4 that DIAS would review, 2 that all countries could review and 1 that NIAB and DIAS would re-analyse. Four markers were rejected.
4)It was AGREED that the markers would be checked by the appropriate laboratory, with the objective of producing an agreed list (target number 20) by the end of May. ACTION: NIAB, GEVES, DIAS.
5)It was also agreed that a draft analytical protocol would be produced, containing information about the SSRs, including allele (band) calling and nomenclature. Dr Lee AGREED to advise on any information relating to the map positions of the selected markers.
6)Varieties:There was a need to select 410 varieties that would be analysed within the project – 190 by NIAB, 190 by GEVES and 70 by DIAS, with a common set of 40 analysed by all three laboratories. It was AGREED that to make the project feasible, the varieties would only include those which are essentially lines (i.e. no hybrids) and fertile (i.e. no male sterile lines).
7)On this basis, it was AGREED that each country would provide a list of their appropriate and relevant varieties to NIAB, by 16 May. The list would be in Excel and would have the variety name, breeder’s reference, any pedigree-type information, the year of listing and any other useful comments (e.g. closely similar varieties). ACTION: All
8)On the basis of this information, NIAB would firstly propose a list of the 40 common varieties, for agreement by all, and secondly would produce suggested lists of the varieties to be analysed by the others. ACTION: NIAB.
9)It was also AGREED that seed samples would be provided from the national reference collections, apart from the common set of 40, which would be provided by the country of testing. For all analyses, the varieties would be coded. Partners were reminded that it might be necessary to obtain formal permission to use the varieties.
10)Morphological Data & Database:The partners would need to agree which morphological data could reliably be used within the project, bearing in mind the need to produce various measures of “distance”. It was AGREED that the characteristics used should be those in the current CPVO TP, and that (subject to agreement by the statisticians), only the “Notes” for each characteristic would be used. All partners agreed to examine their existing sets of data and variety descriptions – what data are available? Over how many years? What can usefully be included?
11)The database format would be based on the principles to be included in the draft UPOV BMT Guidelines, which were currently being drafted by the UK/F.
12)SUMMARY OF NEXT ACTIONS:
a)Markers to be agreedend of May 2005
b)Produce marker protocolend of May 2005
c)Exchange lists of varieties16 May 2005
d)Decide of set of 40 common varietiesend of May 2005
e)Analyse the 40 varieties with the agreed markersend of August 2005
f)Compare & agree the scoring of the 40 varietiesend of August 2005
g)Compile the lists of 410 varietiesend of August 2005
h)Decide which varieties are analysed where
i)And distribute the seeds if necessaryend of August 2005
j)Analyse the 410 varieties with the chosen markers according to the protocol December 2005
k)Sort out the morphological dataDecember 2005
l)End of Phase 1 – second project meetingJanuary 2006
13)Any Other Business:
a)Research Agreements – it was AGREED that GEVES would check the format used for the Research Agreement currently in place with the BSA for another project, and then that format would be used within this project. ACTION: GEVES to inform NIAB; NIAB then to revise Agreements.
b)It was AGREED that a short presentation on the project should be given at the forthcoming UPOV BMT meeting. ACTION: NIAB to draft a short paper for the partners to agree.
Following a visit to the UK WOSR DUS plots, the meeting closed.
Page 1 of 21 May 2005