Name:______McMush

Introduction:
Carbohydrates, proteins, fats, vitamins, and other nutrients provide your body with energy necessary to carry on life activities. These compounds are present in the plants and animals you use as food. In this lab, you will test for specific compounds and then determine if those compounds are present in ordinary foods.

Objective:
To determine the compounds present in food.

Materials
McDonald's cheeseburger Happy Meal (fries + 12 oz cola drink included), beaker,graduated cylinder, test tubes, test tube clamp,hot plate, Benedict's solution, Biuret solution, Indophenol (DPIP) solution, Lugol's iodine solution, 1% silver nitrate solution, blender, cheese cloth, 100 mL graduated cylinder, refrigerator.

Procedure:
Part I - Testing of Known Substances

Protein test:

  1. Place 5 ml of the albumin solution into your test tube.
  2. Add ten drops of Biuret solution.
  3. Observe any color change

Glucose test:

  1. Place 5 ml of the glucose solution into your test tube.
  2. Add 3 ml of Benedict's solution.
  3. Place the tube in a beaker of boiling water and boil for five minutes
  4. Use test tube clamps to hold hot test tubes.
  5. Observe any color change

Starch test:

  1. Place 5 ml of the starch solution into your test tube.
  2. Add 5 drops of Lugol's iodine solution.
  3. Observe any color change

Record your results in a data table:

Food Substance / Reagent test / Results
Gelatin / Biuret solution
Glucose / Benedict's solution
Starch / Lugol's iodine solution

Procedure:
Part II - Testing McMush

  1. Place a Mc Donald’s Happy Meal, including a 12 oz cola in a blender. Add 1 Cup water to cover and blend until you get an emulsion.
  2. Filter half of the mush in to a beaker using cheesecloth.
  3. Predict the substances you will find in the McMush solution. Record your predictions in the data table using a “ +” or “-“.
  4. Repeat the reagent tests you conducted above using 5 ml of the McMush solution.
  5. Describe and record your results.

Food Substance / Prediction / Reagent test / Results
Protein / Biuret Solution
Sugar / Benedict's Solution
Starch / Lugol's solution
  1. To test for percentage of fat, take approximately 100mL of unfiltered McMush and heat thoroughly. Pour into a 100 mL graduated cylinder and place in a refrigerator for several hours or overnight. Record the amount of fat volume here______.
  2. Fill in the following chart

Macromolecule/ Polymer / Monomer / Drawings: / Ele-ments / Functions and examples / Chemical test/ result
Carbohydrate/ polysaccharide
Lipid / No true monomer
Protein/ polypeptide
Nucleic Acids