Supporting information, table 1: primer sequences

Name / Sequence / Gene target / Remarks
Core sequence extension
NGSP-K5T1 / 5’TTGTCCCGATTTCATCAGCCATGTCA3’ / KdGLS
NGSP-K5T2 / 5’GTTGTCCTGATTTCATCAGTCATGTCG3’ / KdFRS
NGSP-K5T3 / 5’GTTATTCCGATTTCTTCAGCCATGTTA3’ / KdLUS
NGSP-K5T4 / 5’GTCTCCCCAATTTCATCAGACATGTTG3’ / KdTAS
NGSP-K5C1 / 5’CTGGACAGTCTTCTAAAACCTGTGAG3’ / KdCAS
3’-end RACE
NGSP-K3T1 / 5’AGGGGTTGACTTCCTACTAAGG 3’ / KdGLS
NGSP-K3T3 / 5’AAGGCGTTGACTTTCTACTAAACA3’ / KdFRS
NGSP-K3T4 / 5’ATGGTTTGCTCTTGAAGGATTGT3’ / KdLUS
NGSP-K3T5 / 5’TACTGAATTCTTGCTAAACACA3’ / KdTAS
NGSP-K3C1 / 5’GCATGTGATTTTCTTCTTTCCAAG3’ / KdCAS
5’-end RACE
NKK5T1 / 5’TAGAACTGTACCAAACATGGTGCTG3’ / KdGLS
NKK5T2 / 5’TAGAACTGTACCAAACATGGTGCTT3’ / KdFRS/KdLUS
KdLUS
NKK5T6 / 5’ATATGTAGCTGAGAGCGGTGCAG3’ / KdTAS
NKK5C1 / 5’CTTCACCGAGCAATCTTAGTGTCAC3’ / KdCAS
Full length cDNAs
KdGLS-F / 5’CTACAGCTTTGCTATCAGCGTC3’ / KdGLS
KdGLS-R / 5’CGTACAAATTACACTTGTTATCATCG3’ / KdGLS
KdFRS-F / 5’CTACAGCTTTGCTATCAGCGTC3’ / KdFRS
KdFRS-R / 5’GCACAAATTACACTTGTTGTCATCT3’ / KdFRS
KdTAS-F / 5’GGTTGAGCTCAGGATGAGTTTTGTTTGGGTAG3’ / KdTAS / SacI site underl.
KdTAS-R / 5’GCTTGCGGCCGCGGTTGGTTTTATTCGTC3’ / KdTAS / Not I site underl.
KdLUS-F / 5’GGAGGAGGTACCAGGATGTGGAAGTTAAAGATAGC3’ / KdLUS / Kpn I site underl.
KdLUS-R / 5’GCAGCAGCGGCCGCGCTATTAGACCATTTTGGT3’ / KdLUS / Not I site underl.
KdCAS-F / 5’TTTGGATCCAACATGTGGAAGCTTAAGATCGCC3’ / KdCAS / BamHI site underl.
KdCAS-R / 5’CAATCTAGATCACGTCTTCATTTGGCCTCG3’ / KdCAS / Xba I site underl.
RT-PCR
KdTAS-1740F / 5’TCCTGGACATAGGAAGAAAGACA3’
KdTAS-2370R / 5’TTGGTGGAGCTTATATTAGCC3’
KdGLS16-1170F / 5’GTAGATACATCACGATCGGGA3’
KdGLS16-1820R / 5’TACTTCCTTCCTTCGATGTCC3’
KdFRS34-1170F / 5’GTAGATACATCGTGATTGGAG3’
KdFRS34-1820R / 5’CACTTCTTTTCTACGATGTAG3’
KdLUS-K3T3 / 5’CCCTACATCGTAGAAAAGAAGTG3’
KdLUS-45-4A / 5’GCCTCGAGGCTATTAGACCATTTTGG3’
KdCAS-1770F / 5’CCTTACGTGGAGTGTACCTCAG3’
KdCAS-2350R / 5’ATGCGTTCAATACCTTGCAGCG3’
q-RT-PCR
KdGLS-1F / 5’CAAGAAATAACTGGGGTGTTCAAA3’
KdGLS-1R / 5’GTTTTGATATAGCCTCCGAACTAA3’
KdFRS-1F / 5’AGAAATAGCTGGAGTGTTCAAG3’
KdFRS-1R / 5’GTTTTGATATAGCCTCTGAATTAG3’
KdTAS-1F / 5’GGAGATAACAGGAGTCTTCATGAA3’
KdTAS-1R / 5’CTTTATGGCTTAGCGTAATGGCAG3’
KdLUS-1F / 5’AGAAATAACTGGAGTGTTTCAGAG3’
KdLUS-1R / 5’CATTTTGGTAGCCTCTGAATGTAA3’

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Supporting information, table 2: Sequences used for phylogenetic analyses

Access No. / Species / Function
At4g15340 / Arabidopsis thaliana / Arabidiol synthase PEN1
At5g48010 / Arabidopsis thaliana / Thalianol synthase PEN4
At5g42600 / Arabidopsis thaliana / Marneral synthase PEN5
At1g78500 / Arabidopsis thaliana / Multifunctional triterpenoid synthase PEN6
At3g45130 / Arabidopsis thaliana / Lanosterol synthase LAS1/PEN7
At1g78970 / Arabidopsis thaliana / Multifunctional triterpenoid synthase LUP1
At1g78960 / Arabidopsis thaliana / Multifunctional triterpenoid synthase LUP2
At1g66960 / Arabidopsis thaliana / Multifunctional triterpenoid synthase LUP5
At2g07050 / Arabidopsis thaliana / Cycloartenol synthase CAS1
AB263204 / Rhizophora stylosa / Multifunctional triterpenoid synthase RsM2
AB289586 / Bruguiera gymnorhiza / Lupeol synthase BgLUS
AB257507 / Kandelia candel / Multifunctional triterpenoid synthase KcMS
DQ268869 / Ricinus communis / Lupeol synthase RcLUS
AB058643 / Luffa cylindrica / Isomultiflurenol synthase LcIMS1
AB037203 / Glycyrrhiza glabra / β-amyrin synthase GgbAS1
AB181244 / Lotus japonicus / β-amyrin synthase OSC1
AB034802 / Pisum sativum / β-amyrin synthase PSY
AF478453 / Medicago truncatula / β-amyrin synthase AMY1
AF478455 / Lotus japonicus / Multifunctional triterpenoid synthase LjAMY2
AB034803 / Pisum sativum / Multifunctional triterpenoid synthase PSM
AB009030 / Panax ginseng / β-amyrin synthase PNY
AB014057 / Panax ginseng / β-amyrin synthase PNY2
AB055512 / Betula platyphylla / β-amyrin synthase BPY
Kalanchoe daigremontiana / Taraxerol synthase KdTAS
AB263203 / Rhizophora stylosa / Multifunctional triterpenoid synthase RsM1
AB206469 / Medicago tirucalli / β-amyrin synthase EtbAS
Kalanchoe daigremontiana / Friedelin synthase KdFRS
Kalanchoe daigremontiana / Glutinol synthase KdGLS
Kalanchoe daigremontiana / Lupeol synthase KdLUS
AB025343 / Olea europaea / Lupeol synthase OEW
AB025345 / Taraxacum officinale / Lupeol synthase TRW
AB181245 / Lotus japonicus / Lupeol synthase OSC3
AB116228 / Glycyrrhiza glabra / Lupeol synthase GgLUS1
AB055511 / Betula platyphylla / Lupeol synthase BPW
AB181246 / Lotus japonicus / Cycloartenol synthase QSC5
AB025968 / Glycyrrhiza glabra / Cycloartenol synthase GgCAS1
D89619 / Pisum sativum / Cycloartenol synthase PSX
AB009029 / Panax ginseng / Cycloartenol synthase PNX
Kalanchoe daigremontiana / Cycloartenol synthase KdCAS
AB033335 / Luffa cylindrica / Cycloartenol synthase LcCAS1
AB055509 / Betula platyphylla / Cycloartenol synthase BPX1
AB009031 / Panax ginseng / Lanosterol synthase PNZ
AB244671 / Lotus japonicus / Lanosterol synthase OSC7

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