N-Terminal Extension Domain

A

CaM-like core

N-terminal extension ▼ domain

Ce Cal-1 MVRFFSSRLA------RDMAIRAERMAIPSNLMQFSEDIIKQLTPEEID

Cb Cal-1 MALRAERMAIPSNLMQFSEDIIKQLTPEEID

Cr Cal-1 MRCQRLGLPTNSKFFLAISPLLSPLHIPPMIAIRAERMAIPSNLMQFSEDIIKQLTPEEID

As Cal-1 MALSAQ-PQSTSNMMQFSEEIIKQLTPEEID

Ll Cal-1 MALSAAR-QQSSNLMQFNEDIIKQLTAEEID

Bm Cal-1 MALSAAR-QQSSSLMQFNEDIIKQLTTEEID

Wb Cal-1 MALSAAR-QQSSSLMQFNEDIIKQLTTEEID

Ac Cal-1 MMVRVHSSSVAL-TRRRIEQRMALAANSAA---RMMQFSEDIIKQLTPEEVD

Hc Cal-1 MGV------MQFSEDVIKQLTPEEVD

Na Cal-1 MALS--HP-ILS------II-QLTPEEVD

Dv Cal-1 MTMVNNS---YTRTLQCSEDIVKQLTPEEVD

Tc Cal-1 ...KYTMKFCSWETAAARAPLYQQSLDCIVVPELYRGGRMAFN-TQPQSTSNMMQFSEEIIKQLTPEEID

Sr Cal-1 ...YKFNLFNFYYGKRDLSTIGARDLKFVRFMKRLYENAMALGA-RCAQNGNVMQYSEEIIKQLTPEEIE

Tt Cal-1 MVSSSSNQITDNLVQQLTPDEVD

Ts Cal-1 ...KEAVSFPLGGSSQRKDFDSLKAERQEKPRSRSISASKSTSCAPMVSSSSSNQITDNLVQQLTPDEVD

Tsp Cal-1 ...LEDVLSASCVRIFAVNNSVVHVQYSIDIVEHLSVRSLSSTAAMVSSSSSSNQVTDNLVNQLTPDEVD

B

CaM-like core

N-terminal extension ▼ domain

Ce Cal-2 MSNDPRR----VSLFNRWASKQTNEPVSGFDGLNEEEIM

Cb Cal-2 MSNDPRR----VSLFNRWASKQNNEPVSGFEGLNEEEIM

Cbri Cal-2 MSNDPRR----VSLFNRWASKQNNEPVSGFEGLNEEEIM

Ac Cal-2 MSSDPRR----VSLFNRWQQKQTDVP-SGLEGINEEELI

Na Cal-2 MSSDPRR----VSLFNRWQQKQTDVP-TGLEGINEEELI

Hc Cal-2 MSSDPRR----VSLFNRWQQKQSDVP-TGLEGINEEELI

Od Cal-2 MHTVKFLDSDMS-DPRR----VSLFNRWQQKQTDVP-TGLEGINEEELI

Pp Cal-2 ...FLFLSDFWSTILLFFIIILQSAVIVMS-DQRK----MSLFNRWKQNTTD---SGLEGISEEELV

Dv Cal-2 MSSDPRR----ITLFNRWQQKQTDVP-SGLEGINEEELI

As Cal-2A MTSN-RK----SSLFNRWKQQST---ADQLEGISEEEMI

Ll Cal-2A MGS--KRK---SSLFNRWKQQNRNE---QFEGITEDEMI

Tc Cal-2A MSNN-RK----MSLFNRWKQQNTAD---QLEGVSEEEMI

Ll Cal-2B MSNN-RK----MSLFSRWKQQTNNE---QLEGVSEEEAI

As Cal-2B MAA-SKR-----SMFARWK---NLTFTDQLTGIREEELT

Tc Cal-2B MSN-NRK----VSLFNRWKQQNANE---NLEGVSEEEAI

Tc Cal-2C MSA-SKN-----SMFARWKNLSFSD---QPTGIHDDELI

Sr Cal-2 MSQN-RK----LSLFNRWKNNNHQEA--ELEGISDEELI

Tt Cal-2 MRKEVESERRDTSLCKGTTSPAKGTSHHRPTAPKKKLRNVRTMNRFKKPNVIPP--ELENITEDELQ

Ts Cal-2 MNRFKKPSVIPP—-ELENITEDELQ

Tsp Cal-2 MNRFRKPNAIPP--EFENITEDELQ

C

N-terminal extension

Ce Cal-3 MSNIFVSDRKIELSAKCFRVSSTKGHKGQQIH

Cbri Cal-3 MSNIFVSDRKIELSAKCFRVASTKGHKGQH-H

Cr Cal-3 MSNIFVSDRKIELSAKCFRVASTKGHKSQ---

CaM-like core

▼ domain

Ce Cal-3 HPTVPETIKFNITHGFPKLPKSFWLSFEATMSRNTKVIMSIMRTRTLQEVFEESKLVISQLTEEEIH

Cbri Cal-3 HPTVPATIKFNIKHGFPKLPKSFWLSFEETMSRNARVIMSVMRTRTLQEVFEESKMVISQLTEEEIH

Cr Cal-3 HPTVPETIKFNITHGFPKIPKSFWLSFEATMSRNTRVIMSVMRTRTLQEVFEESKLVISQLTEEEIY

Cb Cal-3 MTIDQTNAQWKREIRVIMSVMRTRTLQEVFEESKLVISQLTEEEIH

Hc Cal-3 MAA----I-STVQTKTLQAIFMESEEVIRQLTEQEIE

Ac Cal-3 MTA-T----SSIQTRTLQAIFQESEQVIRTLTEEEIE

Pp Cal-3 MSF-TAYI—-QDASRISVWDLEDFYDAFVQFDKVFIY

Sr Cal-3 MS------YDMAKTYHTLLRESEEIISQLTNQEIE

Tc Cal-3 MSA—-AY-----SSRALNNLLQQSEEVISHLTNQEID

D

N-terminal extension

Ce Cal-4 MNRIGPRRRGFRTRQNGNSQQ------KEIVEPVMASGVCVHSVLPFKSAN

Hc Cal-4 MKILCGNALTSC------PDVVQPVLARGTCVHIVGVFQSAI

Ac Cal-4 MVSTSFEAFTLDDLNDAS------KPWCYWKMAP------

Na Cal-4 MLVIPTRSTAITAI------FVYFPIL-GS------

Od Cal-4 MSVVLPAPT------

Sr Cal-4 MPFIILEKDAFGFRIISGLRKRRFRNTKDDFDNPLSILSSLQLPQNSDPRALNAQLNGC-NNKQEIVEPV

Wb Cal-4 ...SKRRSELTFDLSGLTKVLKRTRLTRNESQSGQDMPPSCPSPSQDASLAVPAKGRIVHMVGVFQSPP

Ll Cal-4 ...SKRRSELTFDLSGLTKVLKRTRLIKNESQSGQDVPPSCSSPSQDGSLAVPAKGRIVHMVGVFQSPP

Bm Cal-4 ...SKRRSELTFDLSGLTKVLKRTRLTRNESQSGQDLPPSCPSPSQDASLAVPAKGHIVHMVGVFQSPP

Tc Cal-4 RSHCRHTQTSPQRVVIPVAAKGRIVHIVGVFQTPP

Tsp Cal-4 ...FGRISQESKNRNTVVKTLVSPRL------

Ts Cal-4 ...FARLTTDSRNRDFHKTSNRPTR------

CaM-like core

▼ domain

Ce Cal-4 ------AADCLARRDMYRQGTNQSVCSDADEFTPEELQ

Cr Cal-4 ...VARRDMYRQGTNQSVCSDADEFTQEELQ

Cbri Cal-4 ...MYRQGTNQSVCSDADEFTQEELQ

Hc Cal-4 -----PTADANN------LAPTSDREMHRQYTNNSGSTEAEEFTQEELQ

Na Cal-4 ------APTSDREMHRQFTNNSGSSEAEEFTQEELQ

Od Cal-4 SDREMHRQYTNNSG---SSEAEEFTQEELQEFAQAFKMFDKDGNGTMNIKELGVAMRTLGMNPTEEELQ

Dv Cal-4 MHRQHTNNSG---SSETEEFTQEELQEFAQAFKMFDRDGNGTMNIKELGIAMRTLGMNPTEEELQ

Sr Cal-4 AAKGTCVYLVGVFKSEPDPSKNTDSDLIFGANNSGLKALDSTESR-MHRQFT--SSSNGEEEFSLEELQ

Ac Cal-4 ------TSDREMHRQYTNNSGSSEAEEFTQEELQ

Wb Cal-4 STSTSSSNESNNGGIATTTNSIQPETPPVHNTEPTYSSSAHLDEPKMYRRYTNSSAATEQDDFTPEELQ

Ll Cal-4 STSASSSNESNNGGITTTSNSIQPETPPVHNIEPTYSSSAHLDESKMYRRYTSSSATTEQDDFTPEELQ

Bm Cal-4 STSTSSSNESNNGGIATTTNSIQPETPPVHNTEPTYSSSAHLDEPKIYRRYTNSSAATEQDDFTPEELQ

Tc Cal-4 STSASSDESANSVGGAL------RPSLSCT-----AIGTCNESKMHRQFTNSSNTTDQEEFTAEELQ

Tsp Cal-4 ------NAYTEEELE

Ts Cal-4 ------FDYSDEELE

E

CaM-like core domain ▼ C-terminal extension

Ce Cal-4 IIREVDKDGDEQ------IDLDEFVNMVAPIVSDGTKTD--PFLEPTASTNPPTK

Cr Cal-4 IIREVDKDGDEQ------IDLDEFVNMVAPIVSETNKTD--PFAEPAASANPTTK

Cbri Cal-4 IIREVDKDGDEQ------IDLDEFVNMVAPIVSETNKTD--PFAEPAASANPTTK

Na Cal-4 IIKEVDKDGDEQ------IDYEEFVQMIAPIVSEGNKDD--PFAEPPPAAN--SK

As Cal-4 IIKEVDKDGDEQ------MIAPIVHDGNKDD--PFAEPPKEHSSKKN

Hc Cal-4 IIKEVDKDGDEQ------IDYEEFVQMIAPIVSEGNKDD--PFAEPPPASATTTK

Pp Cal-4 IIKEVDKDGDEQ------IDYEEFVQMIAPIVQEGNKDD--PFAEPQPQ---TAK

Od Cal-4 IIKEVDKDGDEQ------IDYEEFVQMIAPIVSEGNKDD—-PFAEPPPVAN--SK

Dv Cal-4 IIKEVDKDGDEQHFMTTMGERFTEEEVDEIIKEVDKDGDEQVQYPFHVNLLKFCTKKKFQILLSLQK

Sr Cal-4 IIREVDKDGDEQ------IDYEEFVQMIAPIVQEGNNKDD-PFADPEAKTFKDGKHK

Ll Cal-4 IIQEVDKDGDEQ------INYEEFVQMIAPIVHDDAKDD--PFAD-----TSSKK

Bm Cal-4 IIQEVDKDGDEQ------INYEEFVQMIAPIVHDEAKDD--PFAE-----TSSKK

Tc Cal-4 IIREVDKDGDEQ------INYEEFVQMIAPIVHDGNKDD--PFAEPPKEHTSKKN

Ts Cal-4 IIREVDKDGDEQ------INYEEFVQMIAPSVQYYQPDER-PLD

Tt Cal-4 IIREVDKDGDEQ------INYEEFVQMIAPSVRYYQQDDR-TSDL

Tsp Cal-4 IIREVDKDGDEQ------INYEEFVQMIVPSTNRRDNEDNNGTVQ

F

N-terminal extension CaM-like core domain ▼

Ce Cal-5 MGACCS--SKKK------

Cbr Cal-5 MGACCS--SKKK------

Cr Cal-5 MGACCS--SKKK------

Cbri Cal-5 MGACCS--SKKK------

Ac Cal-5 MSHSHRL------

Hc2 Cal-5 MGTCCSRNSRK------

Angc Cal-5 MGTCCSRNSRK--SQ------

Na Cal-5 MGSCCSRNSRK--SQ------

Ts Cal-5 MGNCCG—-RKSKTITTPVSSTQAKMANQDGTQFRK----

Tt Cal-5 MGNCCG--RKSKTITTPVSSTQAKIANQDGTQTTVSERP

Tsp Cal-5 MGNCV---KRISGSENVTSH------

Sr Cal-5 MGNCL---AKKSEAKNVGNPDS------

Pp Cal-5 ...SLHSLLRSFTSNQTFAGNRNRSRNMGSCCSSAASK------PD------

Ll Cal-5 ...VLCSSCSARRRGPPYGIIDNSFTRMGLCCSSNSKSRNTQ------

Bm Cal-5 ...VLCPWCSLDGHELQQYHRQFTPTRMGICCSSNSRSRNTQ------

Dv Cal-5 ...EETFCHGCHDILQCSSHIHYFAAALKSNCTKRRKYFTKRMIEANDGDPH------

G

N-terminal extension CaM-like core domain ▼

Ce Cal-7 MDLSEDEDLLNIEEEGENESQKEE

Cb Cal-7 MDLSDDEDLLMIEEEEEDESQKED

Cr Cal-7 MDLSDDEDLLMIEEEEEDESQKEE

Cbri Cal-7 MDISDDEDLLAIEEEEEDESQKEE

H

N-terminal extension

Ce CAL-7 MDLSEDEDLLNIEEEGENESQKEE

Tc CAL-7 MASAYNETVSADADDAVSAID

Gi ESU42112 MLKVSCLKSSM

Gl EDO76329 M

CaM-like core domain

Ce CAL-7 VDSVIVEDIRRRLFDVFKMFDEDSDGLIETDDVSHVLRSFGLNPSQTELQ--LVSEQTAKKNGRVSFDDLLPRVVLA

Tc CAL-7 DEEDHIEEKRAHVRAIFDTFDQERSGSLDASEVSHFVRCLGFKPTQRDLSR-FLDQLVKDGSSRITFEHLFARLLAV

Gi ESU42112 SLEAYSQVLVRKVGDTFDIFDTEKNGTIDVREVGTVLRSLGVYPTEAQLHSMLLEIQQDEPSMYVSKASFMAVVCRV

Gl EDO76329 SLEAYSQVLVRKVGDTFDIFDTEKNGTIDVREVGTVLRSLGVYPTEAQLHSMLLEIQQDEPSMYVSKASFMAVVCRV

** ****

Ce CAL-7 IQNEEWKDDTPKQIHAAFQVITSNN--YVQKDTLLQLLTSIGEPLTPQEVKQFLNHVSIRANGDIDWVSYVKDTCEM

Tc CAL-7 SENGEWHFGQPIELERAFATLAPQG--TISKEHLMNLLTSRGEALNASEMKELVGHIGVRRNGDVDWQGYIQQVVDY

Gi ESU42112 ISTNEITGPAPDELARAFAVLDPNNTGFLETEQLRVYLTTSGERLNADEMENFSAFATDTETGLVDWRSYLTAVTDI

Gl EDO76329 ISTNEITGPAPDELARAFAVLDPNNTGFLETEQLRVYLTTSGERLNADEMENFSAFATDTETGLIDWRSYLTAVTDI

**

C-terminal extension

Ce CAL-7 ITSRD

Tc CAL-7 TRQNSPSKAYSLQR

Gi ESU42112 LWQNDD

Gl EDO76329 LWQNDD

I

Ce Cal-1 Tc Cal-7 GiESU42112 GlEDO76329

Ce CAL-7 - 31 30 30

Tc CAL-7 31 - 36 35

Gi ESU42112 30 36 - 99

Gl EDO76329 30 35 99 -