ACRF Biomolecular Resource Facility

ACRF Biomolecular Resource Facility

John Curtin School of Medical Research

BRF Phone:+61 2 6125 4326GDU Phone:+61 2 6125 9289Sample Drop-Off Address:Please print this form and complete

BRF Email:U Email:2, Building 131, Garran Rdall information requested. Contact us

BRF Fax:+61 2 6125 9533Australian National Universityif unsure. This form MUST be signed by

Web: GPO Box 334,PI/Lab Head. Bring it to BRF with your

Canberra ACT 2601samples.

Hi Seq 2500 Order Form

BRF-made Library

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Part 1: Contact Information

Date:
Customer name:
Customer address:
Phone (lab):
Phone (mobile):
Email:
PI (or Lab Head) name:
PI (or Lab Head) email:
PI (or Lab Head) signature:
By signing, you acknowledge and accept BRF charges, and terms and conditions.

Part 2: Billing Information

ANU Customers:
Please provide ANU account code.
Non ANU Customers:
A tax invoice will be emailed to the PI (or Lab Head) unless alternative information is provided here.

Part 3: Output

Please choose a data option
 / Analysed by GDU
(Please contact or 6125 9289 for a discussion of method, and for pricing).
 / Purchase 2TB hard drive from BRF ($170)
 / Supply your own hard drive

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Part 4: Libraries and sequence(BRF makes the library)

Please supply your gel picture or Bioanalyzer results.

A. / Experimental design
B. / Sample Information
Sample Submission Date:
Sample Number / Sample 1 / Sample 2 / Sample 3 / Sample 4 / Sample 5 / Sample 6 / Sample 7
Sample Name
Sample Origin (species/tissue)
Sample Type (DNA, RNA, etc)
Bioanalyser RIN (for RNA)
OD 260/280
OD 260/230
Sample Volume L
Concentration (ng/L)

For your reference:

Illumina HiSeq / Input Material Required / Conc’n (ng/L) / Volume(L) / RIN / OD 260/280 / OD 260/280
Genomic DNA Sequence Sample / 3g of Genomic DNA / 100 / 30 / N/A / 1.8 – 2.1 / >1.5
ChIP SequenceSample / 125ng of ChIP DNA / 5 / 25 / N/A / 1.8 – 2.1 / >1.5
Small RNA / 3g of total RNA / 100 / 30 / >8 / 1.8 – 2.1 / >1.5
mRNA Sequence Sample / 3g of total RNA / 100 / 30 / >8 / 1.8 – 2.1 / >1.5
Mate Pair Sequence / 3ug-gel free; 6-8ug-gel method / 100 / 30 / 1.8 – 2.1 / >1.5
Nextera / Nextera XT / Contact BRF / 1.8 – 2.1 / >1.5
C. Library Type (please tick one)
 gDNA /  Exome
capture /  mRNA /  Stranded RNA library /  Small RNA /  Haloplex /  Mate pair /  ChIP library / Nextera/XT
If you have any special library requirements, please describe them here:

Part 4 continued

D.Sequencing Information.
Lane / Sequence length / No. of Sample / Sample Name (Samples with a different index can be mixed and run on one lane)
Example / 75 bp PE / 5 / BRF 1, BRF 2, BRF 3, BRF 5, BRF 12
Lane 1
Lane 2
Lane 3
Lane 4
Lane 5
Lane 6
Lane 7

For your reference, sequence length can be:Please note: Illumina does not support longer read lengths thanbelow.

Single Read / Paired-end Read
50 base single read (50 bp SR) / 50 base paired-end read (75 bp PE)
100 base single read (100 bp SR) / 100 base paired-end read (100 bp PE)
150 base single read (150bp SR) / 150 base paired-end read (150 bp PE)

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