Vector, Library and Strain Request Form

Vector, library and strain request form

George Smith lab

Instructions: We are trying to streamline our up to now pretty haphazard processing of requests for phage display vectors, libraries and E. coli strains (what we call “kits”). To this end, from now on we will accept requests for our standard materials only on the form below, which will allow the information to be downloaded automatically into a database and processed more expeditiously. Open vectors.doc for a detailed description of the available vectors (fUSE55 and f88-4) andtheir parent fd-tet. Open library.doc for a detailded description of the available random peptide libraries in either fUSE5 vector (fUSE5/6mer and fUSE5/15mer libraries) or f88-4 (f88/15mer and Cys0–Cys6 libraries). Open Strains.doc for a detailed description of the availableE. coli strains (K91BK = K91BluKan and MC1061). In light of that information, fill out the last column of the table below, using the correct formatfor each cell (format errors will invalidate the table, in which case the whole request will be ignored without notification). There’s space in the last cell of the last columnfor special instructions,requests for non-standard materials, or othershort text communications (longer, more elaborate communications must be sent in separate e-mails, as explained below). Save the Word document containing the filled out table with a document name having the format “KitRequest*.doc,” where * can be any allowed string (but the document name must begin with the string “KitRequest” or it won’t be processed). Then e-mail the Microsoft Word documentas an attachment to George Smith at , using “KitRequest” as the subject line.The body of the e-mailto which the Word document is attached won’t be read;any communication that’s not in the table within the Word document must be sent in a separate e-mail.

Item and description / Entry format / Entry
Last name, first name (IN THAT ORDER!!) / Text string
e-mail address / Text string
Telephone number / Text string
1st line of mailing address / Text string
2nd line of mailing address / Text string or blank[1]
3rd line of mailing address / Text string or blank1
4th line of mailing address / Text string or blank1
5th line of mailing address / Text string or blank1
6th line of mailing address / Text string or blank1
fUSE5/6mer library virions (vector fUSE5) / 1, 0 or blank[2]
fUSE5/15mer library virions (vector fUSE5) / 1, 0 or blank2
f88/15mer library virions (vector f88-4; IPTG induced) / 1, 0 or blank2
fUSE55 vector RF DNA (replaces fUSE5) / 1, 0 or blank2
f88-4 vector virions (IPTG induced) / 1, 0 or blank2
Cys0 library virions (vector f88-4; IPTG induced) / 1, 0 or blank2
Cys1 library virions (vector f88-4; IPTG induced) / 1, 0 or blank2
Cys2 library virions (vector f88-4; IPTG induced) / 1, 0 or blank2
Cys3 library virions (vector f88-4; IPTG induced) / 1, 0 or blank2
Cys4 library virions (vector f88-4; IPTG induced) / 1, 0 or blank2
Cys5 library virions (vector f88-4; IPTG induced) / 1, 0 or blank2
Cys6 library virions (vector f88-4; IPTG induced) / 1, 0 or blank2
fd-tet virions (parent of fUSE5, fUSE55, f88-4 vectors) / 1, 0 or blank2
K91BK E. coli strain / 1, 0 or blank2
MC1061 E. coli strain / 1, 0 or blank2
fd-cat internal enrichment control phage / 1, 0 or blank2
Special requests, other communications / Text string (can be more than one line long, but should not have any <enter> internally or at the end)

[1] Leave unused address lines blank; stay within the six lines provided

[2]Numeral 1 = requested; numeral 0 or blank (no entry) = not requested; any other entries here may invalidate the entire request.