8
Ying Ge
CV (June 2012)
Curriculum Vitae
Ying Ge
Director of Mass Spectrometry, Human Proteomics Program
School of Medicine and Public Health, University of Wisconsin-Madison
1300 University Avenue, SMI 130, Madison, WI 53706
Phone: (608)263-9212, e-mail:
Web: http://www.humanproteomics.wisc.edu/ge.cfm
EDUCATION
Ph.D. Bioanalytical Chemistry, Cornell University, Ithaca, NY, August 2002
Advisors: Prof. Fred W. McLafferty and Prof. Tadhg P. Begley
Dissertation title: “Top down characterization of proteins by electron capture dissociation and blackbody infrared radiative dissociation mass spectrometry”
B.S. Chemistry, Peking (Beijing) University, China, 1997
PROFESSIONAL POSITIONS
07/2012 Assistant Professor, Department of Cell and Regenerative Biology,
School of Medicine and Public Health, University of Wisconsin-Madison
05/2006 to present Director of Mass Spectrometry, Human Proteomics Program,
School of Medicine and Public Health, University of Wisconsin-Madison
11/2004 to 04/2006 Group Leader of Biological Mass Spectrometry,
Department of Analytical Development, PPD, Inc.
01/2004 to 10/2004 Senior Research Scientist I
Department of Chemical Technologies, Wyeth Research
07/2002 to 12/2003 Research Scientist III
Department of Chemical Technologies, Wyeth Research
SELECTED HONORS AND AWARDS
The Academy of Cardiovascular Research Excellence Young Investigator Award, 2011
American Heart Association Scientist Development Award, 2007-2010
University of Kentucky Cardiovascular Research Center Visiting Professor, 2009
Canadian Society for Mass Spectrometry Student Travel Award, 2001
North American FT-ICR Society Student Travel Award, 2001
PROFESSIONAL ACTIVITIES
Professional affiliations
American Society for Mass Spectrometry (Since 1998)
Human Proteome Organization (Since 2006)
The Association of Biomolecular Resource Facilities (Since 2007)
American Heart Association (Since 2007)
International Society for Heart Research (Since 2009)
The Academy of Cardiovascular Research Excellence (Since 2011)
Proposal review activities
NIH Special Emphasis Panel Reviewer (Cardiovascular and Respiratory Sciences)
NIH Study Section Ad Hoc Reviewer (Myocardial Ischemia and Metabolism)
National Science Foundation Major Research Instrumentation (MRI) Program
Canada Foundation for Innovation Leaders Opportunity Fund
Ontario Research Fund - Global Leadership Round in Genomics & Life Sciences Competition
Experimental Program to Stimulate Competitive Research and Institutional Development Award Programs
Pacific Northwest National Laboratory EMSL Peer Review Panel
Journal peer-review activities
Analytical Chemistry, Molecular and Cellular Proteomics, Circulation Cardiovascular Genetics, Journal of the American Society for Mass Spectrometry, Journal of Proteome Research, Proteomics, Proteomics-clinical application, International Journal of Mass Spectrometry, Chemical Research in Toxicology
Conference organizer
Human Proteomics Symposium (2006, 2007)
Proteomics workshop (2010)
Wisconsin Human Proteomics Symposium (2011)
PUBLICATIONS
at UW-Madison
37. Oliveira, S.M.; Zhang, Y.H.; Sancho Solís, R.; Isackson, H.; Bellahcene, M.; Yavari, A.; Pinter, K.; Davies, J.D., Ge, Y.; Ashrafian, H.; Walker, J. W.; Carling, D.; Watkins, H.; Casadei, B.; Redwood, C. AMP-activated protein kinase phosphorylates cardiac troponin I and alters contractility of murine ventricular myocytes, Circ. Res., Epub ahead of print.
36. Valkevich, E. M.; Guenette, R. G.; Sanchez, N. A.; Chen, Y.; Ge, Y.; Strieter, E. R. Forging isopeptide bonds using thiol-ene chemistry: site-specific coupling of ubiquitin molecules for studying the activity of isopeptidases, J. Am. Chem. Soc. 2012, 134, 6916-6919.
35. Peng, Y.; Chen, X.; Sato, T., Rankin, S. A.; Tsuji, R. F.; Ge, Y. Rapid Purification and High-Resolution Top-down Mass Spectrometric Characterization of Human Salivary α-Amylase, Anal. Chem. 2012, 84, 3339-3346. (Ge, Y. Corresponding author)
34. Du, J.; Teng, R. J.; Lawrence M.; Guan, T.; Ge, Y.; Shi, Y. The protein partners of GTP cyclohydrolase I (GCH1) in rat organs, Plos One, 2012, 7, e33991.
33. Dong, X.; Sumandea, C. A.; Chen, Y.; Garcia-Cazarin, M. L.; Zhang, J.; Balke, C. M.; Sumandea, M. P.; Ge, Y. Augmented phosphorylation of cardiac troponin I in hypertensive heart failure, J. Biol. Chem. 2012, 287, 848-857. (Ge, Y. Corresponding author)
32. Wang, Y.; Pinto, J. R.; Sancho Solis, R.; Liang, J.; Diaz-Perez, Z.; Harada, K.; Ge, Y.; Walker, J. W. Potter, J. D. The generation and functional characterization of knock in mice harboring the cardiac Troponin I-R21C mutation associated with hypertrophic cardiomyopathy, J. Biol. Chem. 2012, 287, 2156-2167.
31. Zhang, H.; Ge, Y. Comprehensive analysis of protein modifications by top-down mass spectrometry, Circ. Cardiovasc. Genet. 2011, 4, 711. *This is an invited review for “Integrating proteomics into cardiovascular disease – an educational review series”. (Ge, Y. Corresponding author)
30. Zhang, J.; Guy, J. M.; Norman, H. A.; Chen, Y.; Dong, X.; Wang, S.; Kohmoto, T.; Young, K. H.; Moss, R. L.; Ge, Y. Top-Down quantitative proteomics identified phosphorylation of cardiac troponin I as a candidate biomarker for chronic heart failure, J. Proteome. Res. 2011, 10, 4054-4065. (Ge, Y. Corresponding author)
29. Zhang, J.; Ayaz-Guner, S.; Dong, X.; Xu, Q.; Guner, H.; Ge, Y. Phosphorylation, but not alternative splicing or proteolytic degradation, is conserved in human and mouse cardiac troponin T, Biochemistry, 2011, 50, 6081-6092. (Ge, Y. Corresponding author)
28. Sancho Solis, R.; Ge, Y.; Walker, J. W. A preferred AMPK phosphorylation site in the inhibitory loop of cardiac and skeletal troponin I, Protein Sci. 2011, 20, 894-907. (Ge, Y. Corresponding author)
27. Kuhn, P.; Chumanov, R.; Wang, Y.; Ge, Y.; Burgess, R. R.; Xu, W. Automethylation of CARM1 allows coupling of transcription and mRNA splicing, Nucl. Acids Res. 2011, 39, 2717-2726.
26. Xu, F.; Xu, Q.; Dong, X.; Guy, M.; Guner, H.; Hacker, T. A.; Ge, Y. Top-down high-resolution electron capture dissociation mass spectrometry for comprehensive characterization of post-translational modifications in rhesus monkey cardiac troponin I, Int. J. Mass Spectrom. 2011, 305, 95-102. *This is an invited article. (Ge, Y. Corresponding author)
25. Nelson, C. A.; Szezech, J. R.; Dooley, C. J.; Xu, Q.; Lawrence, M. J.; Zhu, R.; Jin, S.; Ge, Y. Effective enrichment and mass spectrometry analysis of phosphopeptides using mesoporous metal oxide nanomaterials, Anal. Chem. 2010, 82, 7193-7201. (Ge, Y. Corresponding author)
24. Zhang, J.; Dong, X.; Hacker, T. A.; Ge, Y. Deciphering modifications in swine cardiac troponin I by Top-down high-resolution tandem mass spectrometry, J. Am. Soc. Mass. Spectrom. 2010, 21, 940-948. (Ge, Y. Corresponding author) * This is an invited article featured on the cover of the journal.
23. Ayaz-Guner, S.; Zhang, J.; Li, L.; Walker, J. W.; Ge, Y. In vivo phosphorylation site mapping in mouse cardiac troponin I by high resolution top-down electron capture dissociation mass spectrometry: Ser22/23 are the only sites basally phosphorylated, Biochemistry, 2009, 48, 8161-8170. (Ge, Y. Corresponding author)
22. Ge, Y.; Rybakova, I.; Xu, Q.; Moss, R. L. Top-down high resolution mass spectrometry of cardiac myosin binding protein C revealed that truncation alters protein phosphorylation state, Proc. Natl. Acad. Sci. U. S. A. 2009,106, 12658-12663. (Ge, Y. Corresponding author) *This article is a PNAS Direct Submission (Track II).
21. Kuhn, P.; Xu, Q.; Cline, E.; Zhang, D.; Ge, Y.; Xu, W. Delineating Anopheles gambiae coactivator associated arginine methyltransferase 1 (AgCARM1) by top-down high resolution tandem mass spectrometry, Protein Sci. 2009, 18, 1272-1280. (Ge, Y. Corresponding author)
20. Du, J.; Wei, N; Xu, H.; Ge, Y.; Vasquez-Vivar, J.; Guan, T.; Oldham, K. T.; Pritchard, K. A.; Shi, Y. Identification and functional characterization of phosphorylation sites on GTP cyclohydrolase I, Arterioscler. Thromb. Vasc. Biol. 2009, 29, 2161-2168.
19. Nelson, C. A.; Szczech, J. R.; Xu, Q.; Jin, S.; Ge, Y. Mesoporous Zirconium oxide nanomaterials effectively enrich phosphopeptides for mass spectrometry-based phosphoproteomics, Chem. Commun. 2009, 6607-6609. (Ge, Y. Corresponding author)
18. Liu, L.; Hou, J.; Du, J.; Chumanov, R. S.; Xu, Q.; Ge, Y.; Johnson, J.A.; Murphy, R.M. Differential modification of Cys10 alters transthyretin’s effect on beta-amyloid aggregation and toxicity, Protein Eng. Des. Sel. 2009, 1–10.
17. Ma, M.; Chen, R.; Ge, Y.; He, H; Marshall, A. G.; Li, L. Combining bottom-up and top-down mass spectrometric strategies for de novo sequencing of the Crustacean hyperglycemic hormone (CHH) from Cancer borealis, Anal. Chem. 2009, 81, 240-247.
16. Sancho Solis, R.; Ge, Y.; Walker, J. W. Single amino acid sequence polymorphisms in rat cardiac troponin revealed by top-down tandem mass spectrometry, J. Muscle Res. Cell Motil. 2008, 29, 203-212.
15. Zabrouskov, V.; Ge, Y.; Schwartz, J.; Walker, J. W. Unraveling molecular complexity of phosphorylated human cardiac troponin I by top down electron capture dissociation/electron transfer dissociation mass spectrometry, Mol. Cell. Proteomics 2008, 7, 1838-1849. (Ge, Y. Corresponding author)
Prior to UW-Madison
14. Colabroy, K. L.; Zhai, H. L.; Li, T. F.; Ge, Y.; Zhang, Y.; Liu, A. M.; Ealick, S. E.; McLafferty, F. W.; Begley, T. P., The mechanism of inactivation of 3-hydroxyanthranilate-3,4-dioxygenase by 4-chloro-3-hydroxyanthranilate. Biochemistry 2005, 44, 7623-7631.
13. Xiang, J.; Ipek, M.; Suri, V.; Massefski, W.; Pan, N.; Ge, Y.; Tam, M.; Xing, Y.; Tobin, J.; Xu, X.; Tam, S. Synthesis and biological evaluation of sulfonamidooxazoles and b-keto sulfones: selective inhibitors of 11b–hydroxyster oid dehydrogenase type I, Bioorg. Med. Chem. Lett. 2005, 15, 2865-2869.
12. Ge, Y.; Lawhorn, B. G.; ElNaggar, M.; Sze, S. K.; Begley, T. P.; McLafferty, F. W. Protein oxidation in viral prolyl-4-hydroxylase characterized by top down high resolution tandem mass spectrometry, Protein Sci. 2003, 12, 2320-2326.
11. Ge, Y.; ElNaggar, M.; Sze, N.; Oh, H.; Begley, T. P.; McLafferty, F. W.; Boshoff, H.; Barry, C. B. Top down characterization of secreted proteins from mycobacterium tuberculosis by electron capture dissociation mass spectrometry J. Am. Soc. Mass Spectrom. 2003, 14, 253-261.
10. Sze, S. K.; Ge, Y.; Oh, H.; McLafferty, F. W. Plasma electron capture characterization of large dissociation for the proteins by top down mass spectrometry Anal. Chem. 2003, 75, 1599-1603.
9. Oh, H.; Bruker, K.; Sze, S. K.; Ge, Y.; McLafferty, F. W. Secondary and tertiary structure of gaseous ions characterized by electron capture dissociation mass spectrometry and photofragment spectroscopy, Proc. Natl. Acad. Sci. U. S. A. 2002, 99, 15863-15868.
8. Sze, S. K.; Ge, Y.; Oh, H.; McLafferty, F. W. Top down mass spectrometry of a 29 kDa protein for characterization of any posttranslational modification to within one residue, Proc. Natl. Acad. Sci. U. S. A. 2002, 99, 1774-1779.
7. Ge, Y.; Lawhorn, B. G.; ElNaggar, M.; Strauss, E.; Park, J-H.; Begley, T. P.; McLafferty, F. W. “Top down” characterization of larger proteins (45 kDa) by electron capture dissociation mass spectrometry, J. Am. Chem. Soc. 2002, 124, 672-678.
6. Xi, J.; Ge, Y.; Kinsland, C. L.; McLafferty, F. W.; Begley, T. P. Biosynthesis of the thiazole moiety of thiamin in Echeriachia coli: identification of a novel acyldisulfide linked protein-protein conjugate that is functionally analogous to the ubiquitin/E1 complex, Proc. Natl. Acad. Sci. U. S. A. 2001, 98, 8513-8518.
5. Ge, Y.; Horn, D. M.; McLafferty, F. W. Blackbody infrared radiative dissociation of larger (42 kDa) multiply charged proteins, Int. J. Mass Spectrom. 2001, 210/211, 203-214.
4. McLafferty, F. W.; Horn, D. M.; Ge, Y.; Cerda, B.; Fridriksson, E.; Breuker, K. New frontiers in molecular mass spectrometry, Adv. Mass Spectrom. 2001, 67, 167-173.
3. McLafferty, F. W.; Horn, D. M.; Breuker, K.; Ge, Y; Cerda, B., A. Electron capture dissociation of gaseous multiply charged ions by FTICR, J. Am. Soc. Mass Spectrom. 2001, 12, 245-249.
2. Strauss, E.; Kinsland, C.; Ge, Y.; McLafferty, F. W.; Begley, T. P. The identification and characterization of phosphopantothenoylcysteine synthetase from E. coli completes the identification of coenzyme a biosynthetic enzymes in bacteria, J. Biol. Chem. 2001, 276, 13513.
1. Horn, D. M.; Ge, Y.; McLafferty, F. W. Activated ion electron capture dissociation for mass spectral sequencing for larger (42 kDa) proteins, Anal. Chem. 2000, 72, 4778-4784.
PATENTS
1. Jin. S.; Ge, Y.; Nelson, C. A.; Xu, Q. Mesoporous metal oxide materials for phosphorproteomics, PCT/US09/56632 and 12557906, pending.
INVITED TALKS
· Harvard Medical School, Proteomics Center at Children’s Hospital. To be scheduled.
· University of Cincinnati, Department of Pharmacology and Cell Biophysics. To be scheduled.
· Department of Pharmaceutical Sciences, State University of New York, Buffalo, NY. To be scheduled.
· University of Oklahoma Health Science Center, Department of Medicine. To be scheduled.
· 2013 ASMS Sanibel Conference on Top Down Proteomics, St. Pete Beach, FL. (Jan. 2013) Scheduled.
· Loyola University Chicago, Stritch School of Medicine, Department of Cell and Molecular Physiology, Chicago, IL. (Nov. 2012) Scheduled.
· Aurora Health Care, Patient Centered Research, Milwaukee, WI. (Aug. 2012) Scheduled.
· National Yang Ming University, Division of Cardiology of the Department of Internal Medicine, Taipei, Taiwan. (June 2012) Scheduled.
· Chang Gung University and Chang Gung Memorial Hospital, Taipei, Taiwan. (Jun. 2012) Scheduled.
· National Taiwan University, College of Medicine. (Jun. 2012) Scheduled.
· The 4th World Chinese Mass Spectrometry Conference (WCMSC), Cheng Kung University, Tainan, Taiwan (Jun. 2012) Scheduled.
· Peking University, College of Chemistry and Chemical Engineering, Department of Chemical Biology, Beijing, China, (Jun. 2012) Scheduled.
· Beijing Normal University, Department of Chemistry, Beijing, China (June 2012) Scheduled.
· The third international meeting on myofilaments: Myofilament proteins as structural scaffolds and mediators of function in muscle, Madison, WI. (May 2012)
· Experimental Biology Meeting 2012, San Diego, CA. (Apr. 2012)
· Pittcon 2012, "Quantitation of Proteins: From Clinical Applications to Biotherapeutics." Orlando, FL. (Mar. 2012)
· Boston Biomedical Research Institute, Watertown, MA. (Apr. 2012).
· The Inaugural Workshop for Consortium for Top-Down Proteomics, Cascais, Portugal. (Mar. 2012)
· University of Illinois at Urbana-Champaign, Department of Chemistry, Urbana-Champaign, IL. (Jan. 2012)
· Eli Lilly and Company, MusculoSkeletal Research Seminar Series, Indianapolis, IN. (Dec. 2011)
· Iowa State University, Department of Biochemistry, Biophysics and Molecular Biology. (Nov. 2011)
· Georgia Institute of Technology, School of Chemistry and Biochemistry, Atlanta, GA. (Nov. 2011)
· University of Wisconsin-Madison, Department of Cell and Regenerative Biology, Madison, WI. (Nov. 2011)
· The 14th Beijing Conference and Exhibition on Instrumental Analysis (BCEIA), Beijing, China. (Oct. 2011) Declined.
· 2011 Wisconsin Human Proteomics Symposium, Madison, WI. (Aug. 2011)
· 8th North American FT MS Conference, Key West, FL. (May 2011)
· University of California, Irvine, Department of Physiology and Biophysics. (Apr. 2011)
· University of California at Los Angeles, UCLA Proteomics Seminar Series. (Apr. 2011)
· University of California at Riverside, Department of Chemistry Analytical Seminar Series. (Apr. 2011)
· Washington University in St. Louis, NIH NCRR Biomedical Mass Spectrometry Resource and Midwest Mass Spectrometry Discussion Group. (Oct. 2010)
· University of Wisconsin-Madison, Molecular Hematology Seminar Series. (Oct. 2010)
· Medical College of Wisconsin, Department of Pediatric Surgery. (Sept. 2010)
· University of Kentucky, Cardiovascular Seminar Series. (Oct. 2009)