Supplementary Table 2: Statistical analysis of 3RRD evaluations obtained from 3D-FISH experiments (ARR=average relative radius; sem=standard error of the mean, P-values in red = statistically significant, P-values in blue = statistically not significant)

A) gene-density correlated radial arrangement according to Figure 2.

Human lymphoblastoid cell nuclei
ARR / sem
gene-dense / 58,39 / 0,47
Intermediate / 60,94 / 0,49
gene-poor / 65,36 / 0,7
Mann-Whitney Rank Sum Test (U-test)
gene-dense vs. gene-poor / P = <0,001
Intermediate vs. gene-dense / P = 0,006
Intermediate vs. gene-poor / P = <0,001
Orangutan lymphoblastoid cell nuclei
ARR / sem
gene-dense / 58,22 / 0,4
Intermediate / 62,98 / 0,41
gene-poor / 65,64 / 0,42
Mann-Whitney Rank Sum Test (U-test)
gene-dense vs. gene-poor / P = <0,001
Intermediate vs. gene-dense / P = <0,001
Intermediate vs. gene-poor / P = <0,001
Gibbon lymphoblastoid cell nuclei
ARR / sem
gene-dense / 57,79 / 0,61
Intermediate / 58,62 / 0,68
gene-poor / 62,39 / 0,72
Mann-Whitney Rank Sum Test (U-test)
gene-dense vs. gene-poor / P = <0,001
Intermediate vs. gene-dense / P = 0,252
Intermediate vs. gene-poor / P = <0,001
Orangutan fibroblast nuclei
ARR / sem
gene-dense / 56,75 / 0,49
Intermediate / 57,84 / 0,43
gene-poor / 59,2 / 0,47
Mann-Whitney Rank Sum Test (U-test)
gene-dense vs. gene-poor / P = <0,001
Intermediate vs. gene-dense / P = 0,115
Intermediate vs. gene-poor / P = 0,028
Wolf`s guenon fibroblast nuclei
ARR / sem
gene-dense / 57,50 / 0,91
Intermediate / 59,93 / 1,05
gene-poor / 68,46 / 0,78
Mann-Whitney Rank Sum Test (U-test)
gene-dense vs. gene-poor / P = <0.001
Intermediate vs. gene-dense / P = 0.084
Intermediate vs. gene-poor / P = <0.001

B) chromosome size correlated radial arrangement according to Figure 3.

Human fibroblast nuclei
ARR / sem
Large chr. 1, 3-6 / 69,53 / 0,78
small chr. 16-20 / 48,18 / 1,16
NOR chr. 13-15, 21, 22 / 54,46 / 1,05
Mann-Whitney Rank Sum Test (U-test)
Large vs. small chr. / P = <0,001
Large vs. NOR chr. / P = <0,001
small vs. NOR / P = <0,001
Gorilla fibroblast nuclei
ARR / sem
Large chr. 1, 3-6 / 65,96 / 0,80
small chr. 16-20 / 50,25 / 1,48
NOR chr. 13-15, 21, 22 / 51,47 / 1,18
Mann-Whitney Rank Sum Test (U-test)
Large vs. small chr. / P = <0,001
Large vs. NOR chr. / P = <0,001
small vs. NOR / P = 0,715
Orangutan fibroblast nuclei
ARR / sem
Large chr. 1, 3-6 / 63,15 / 0,91
small chr. 16-20 / 50,87 / 0,90
NOR chr. 13-15, 21, 22 / 53,36 / 1,06
Mann-Whitney Rank Sum Test (U-test)
Large vs. small chr. / P = <0,001
Large vs. NOR chr. / P = <0,001
small vs. NOR / P = 0,121
Wolf`s fibroblast nuclei
ARR / sem
Large chr. 1, 3-6 / 58,32 / 0,74
small chr. 16-20 / 60,06 / 1,20
NOR chr. 13-15, 21, 22 / 58,30 / 1,07
Mann-Whitney Rank Sum Test (U-test)
Large vs. small chr. / P = 0,090
Large vs. NOR chr. / P = 0,774
small vs. NOR / P = 0,248
Orangutan lymphoblastoid cell nuclei
ARR / sem
Large chr. 1, 3-6 / 59,64 / 1,03
small chr. 16-20 / 50,06 / 1,14
NOR chr. 13-15, 21, 22 / 55,57 / 1,25
Mann-Whitney Rank Sum Test (U-test)
Large vs. small chr. / P = <0,001
Large vs. NOR chr. / P = 0,021
small vs. NOR / P = 0,004
C) Impact of fusions, fissions and translocations on individual chromosomes according to Figure 4.
Wolf`s guenon fibroblast nuclei
ARR / sem
chr. 18 / 67,62 / 2,54
chr. 19 / 57,53 / 1,9
Mann-Whitney Rank Sum Test (U-test)
chr. 18 vs. chr. 19 / P = 0,004
Human fibroblast nuclei
ARR / sem
chr. 2pter-q13 / 71,34 / 1,38
chr. 2q13-qter / 80,42 / 1,08
Mann-Whitney Rank Sum Test (U-test)
chr. 2pter-q13 vs. chr. 2pter-q13 / P = <0,001
Gorilla fibroblast nuclei
ARR / sem
chr. 2pter-q13 / 62,33 / 2,52
chr. 2q13-qter / 72,23 / 2,29
Mann-Whitney Rank Sum Test (U-test)
chr. 2pter-q13 vs. chr. 2pter-q13 / P=0.013
Orangutan lymphoblastoid cell nuclei
ARR / sem
chr. 5 / 64,32 / 1,51
chr. 17 / 52,08 / 1,08
Mann-Whitney Rank Sum Test (U-test)
chr. 5 vs. chr. 17 / P = <0,001
Gorilla lymphoblastoid cell nuclei
ARR / sem
chr. 5 / 67,28 / 1,40
chr. 17 / 53,13 / 0,99
Mann-Whitney Rank Sum Test (U-test)
chr. 5 vs. chr. 17 / P = <0,001
Orangutan fibroblast nuclei
ARR / sem
chr. 5 / 65,84 / 1,52
chr. 17 / 52,26 / 1,68
Mann-Whitney Rank Sum Test (U-test)
chr. 5 vs. chr. 15 / P = <0,001
Gorilla fibroblast nuclei (both translocation products)
ARR / sem
chr. 5 / 62,08 / 1,57
chr. 17 / 58,57 / 1,48
Mann-Whitney Rank Sum Test (U-test)
chr. 5 vs. chr. 17 / P = 0,102
Gorilla fibroblast nuclei (152,1 Mbp large translocation product)
ARR / sem
chr. 5 / 70,21 / 3,21
chr. 17 / 61,09 / 2,65
Mann-Whitney Rank Sum Test (U-test)
chr. 5 vs. chr. 17 / P=0,038
D) Impact of fusions and fissions in New World monkeys according to Figure 5.
Human lymphoblastoid cell nuclei
ARR / sem
chr. 13 / 66,05 / 1,65
chr. 17 / 44,65 / 1,80
chr. 20 / 51,56 / 1,64
Mann-Whitney Rank Sum Test (U-test)
chr. 13 vs. chr. 17 / P = <0,001
chr. 13 vs. chr. 20 / P = <0,001
chr. 17 vs. chr. 20 / P = 0,020
Marmoset lymphoblastoid cell nuclei
ARR / sem
chr. 13 / 71,20 / 1,11
chr. 17 / 55,99 / 1,23
chr. 20 / 57,42 / 1,48
Mann-Whitney Rank Sum Test (U-test)
chr. 13 vs. chr. 17 / P = <0,001
chr. 13 vs. chr. 20 / P = <0,001
chr. 17 vs. chr. 20 / P = 0,465
Human lymphoblastoid cell nuclei
ARR / sem
1p34-pter / 38,73 / 2,05
1q32-qter / 58,16 / 1,66
Mann-Whitney Rank Sum Test (U-test)
1p34-pter vs. 1q32-qter / P = <0,001
Marmoset lymphoblastoid cell nuclei
ARR / sem
1p34-pter / 48,96 / 1,31
1q32-qter / 56,17 / 1,58
Mann-Whitney Rank Sum Test (U-test)
1p34-pter vs. 1q32-qter / P = 0,001

E) NOR bearing chromosomes and heterochromatin according to Figure 6.

Human fibroblast nuclei
ARR / sem
NOR pos. chr. 13 / 68,30 / 2,94
NOR pos. chr. 14 / 66,61 / 1,93
NOR pos. chr. 15 / 60,41 / 2,14
NOR pos. chr. 21 / 50,48 / 2,56
NOR pos. chr. 22 / 52,91 / 2,11
Gorilla fibroblast nuclei
ARR / sem
NOR neg. chr. 13 / 60,00 / 2,64
NOR neg. chr. 14 / 59,34 / 2,82
NOR neg. chr. 15 / 59,16 / 2,68
NOR pos. chr. 21 / 51,02 / 2,55
NOR pos. chr. 22 / 49,15 / 2,15
Mann-Whitney Rank Sum Test (U-test)
Human chr. 13 vs. gorilla chr. 13 / P = 0,070
Human chr. 14 vs. gorilla chr. 14 / P=0,145
Human chr. 15 vs. gorilla chr. 15 / P=0,910
Human chr. 21 vs. gorilla chr. 21 / P=0,546
Human chr. 22 vs. gorilla chr. 22 / P=0.990
Squirrel monkey lymphoblastoid cell nuclei
ARR / sem
chr. 19 / 50,96 / 1,9
Heterochromatin / 69,79 / 1,51
1q32-qter -heter. / 56,19 / 2,63
1q32-qter +heter. / 67,87 / 1,98
Mann-Whitney Rank Sum Test (U-test)
1q32-qter –heter. vs. 1q32-qter +heter. / P = 0,002
1q32-qter –heter. vs. heterochr. / P = <0,001
1q32-qter +heter. vs. heterochr. / P = 0,877