Supplemental table 1: Oligonucleotides

Description / Template / Namea / Sequence
Oligonucleotides used for mutagenesis
Left flanking sae fragment / ISP479C / A: Kpnsae-for / CGGGGTACCATACTACAGTTTTACATT
ISP479C / B: Hybridsae-rechts / taaaagttcgctagataggggtcccaaagaagctcccattaatgagggc
Right flanking sae fragment / ISP479C / C: Hybridsae-links / TCCAATTCTCGTTTTCATACCTCGGAGCTAACTCCTCATTTCTTCAATTT
ISP479C / D: Kpnsae-rev / CGGGGTACCTGTTGGTGATTTTAGACT
Kanamycin resistance gene aphIII / pDG782 / E: KanR for / ccgaggtatgaaaacgagaattgg
pDG782 / F: KanR rev / GGGACCCCTATCTAGCGAACTTT
Oligonucleotides used for complementation
saePQRSL / ISP479C / sae-1U / GCGTGAATTCTTATTGTGGCAAAAGGTTT
sae-3515L / CCCCGAATTCCTGTATGCCGCCTAATAAT
saePQRSP / Newman / sae-1U / GCGTGAATTCTTATTGTGGCAAAAGGTTT
sae-3515L / CCCCGAATTCCTGTATGCCGCCTAATAAT
saeRSL / ISP479C / sae-1509U / CTTTGAATTCAGAGGTCGTAAGAACAGAGG
sae-3449L / TATTGAATTCGAAGTTAAGGCACGTCCT
saeRSP / Newman / sae-1509U / CTTTGAATTCAGAGGTCGTAAGAACAGAGG
sae-3449L / TATTGAATTCGAAGTTAAGGCACGTCCT
saePQR / ISP479C / sae-1U / GCGTGAATTCTTATTGTGGCAAAAGGTTT
2267hybrid / GTTATTTTGTTGCTGATTTTTGCTACGACGCCAATAATGAT
3218hybrid / GAAGTCAAATCATTATTGGCGTCGTTAACGCAAAAATCAGTGTAAGC
sae-3515L / CCCCGAATTCCTGTATGCCGCCTAATAAT
saePQRD51NSL (mutated phosphorylation site) / RN6390 / sae-1U / GCGTGAATTCTTATTGTGGCAAAAGGTTT
1680L29 / CTTCTGGCATCATGATATTAAGTACCATG
1684U31 / gtacttAatatcatgatgccagaagttaatg
sae-3515L / CCCCGAATTCCTGTATGCCGCCTAATAAT
saePQRD51NSP (mutated phosphorylation site) / Newman / sae-1U / GCGTGAATTCTTATTGTGGCAAAAGGTTT
1680L29 / CTTCTGGCATCATGATATTAAGTACCATG
1684U31 / gtacttAatatcatgatgccagaagttaatg
sae-3515L / CCCCGAATTCCTGTATGCCGCCTAATAAT
saeRD51NSL (mutated phosphorylation site) / pCWSAE56 / sae-1509U / CTTTGAATTCAGAGGTCGTAAGAACAGAGG
sae-3515L / CCCCGAATTCCTGTATGCCGCCTAATAAT
saeRD51NSP (mutated phosphorylation site) / pCWSAE59 / sae-1509U / CTTTGAATTCAGAGGTCGTAAGAACAGAGG
sae-3515L / CCCCGAATTCCTGTATGCCGCCTAATAAT
Oligonucleotides used for lacZ-fusions
capAP-Promotor / Cap8P-for / TTAAGAATTCCTAATCCTAAAGAAGCACTAA
Cap8P-rev / TTAAGAATTCGTACTTTCCAATATTTACCTC
Oligonucleotides used for vector manipulation
attP exchange / EcoRIphi13attP-for / CACAGAATTCACCCATTAGGGACTCCAA
Salphi13attP-rev / CACAGTCGACTGTACATTCCACACAAAC
pCG32 / EcoRIphi13int-for / TTTTGAATTCGGATTAAATACAAGCACTG
SalIphi13int-rev / CACCGTCGACCCCTAAATTACATACTTTCTAACAT
Oligonucleotides used for verification
tetR-for / CAATACATTGTAGGCTGC
sae-1585L / TGTCTACAATGTCTTGTT
sae-564L / ACCTTTTGATGATTTGTAGTTAG
sae1980u / TGGTCACGAAGTCCCTATGC
sae2584L / TGCTTGCGTAATTTCCGTTAG
sae-3515L / CCCCGAATTCCTGTATGCCGCCTAATAAT

a: U: upper primer, L: lower primer.

Bold: artificial restriction sites

Underlined: overlapping region,

Bold and italic: Asp to Asn exchange


Supplemental table 1b: Oligonucleotides used for construction of hybridization probes

saeR/S / AJ556795 / sae1980U / TGGTCACGAAGTCCCTATGC
sae2458L / TGCTTGCGTAATTTCCGTTAG
saeR / AJ556795 / sae1621 / CAAAGAATTCGTAACAACGACAACTAGCGG
saeR2 / tgaaaggagccgataatgg
fnbA / J04151 / fnbAU / TGCAAATACGACAGATACTT
fnbAL / TTGGCCACCTTCATAACCTA
coa / X16457 / coaU / cgagaccaagattcaacaag
coaL / aaagaaaaccactcacatca
hla / X01645 / hlaU / AGAAAATGGCATGCACAAAAA
hlal / tgtagcgaagtctggtgaaaa
hlb / X61716 / hlb-258 / ATTAGTTGGTGCACTTACTG
hlb-675 / GCTATCATTATCGAATCCAC
eap / AJ290973 / map-98 / AATAATAATGAAGCGTCTGC
map-650 / CGGTAATACCTCTATTTGATT