Supplemental Figure 1. Example diel oxygen saturation curve for open and closed reaches from Threemile Creek, Summer 2014.

Supplemental Table 1. Estimates of k, GPP, ER, and NEP for each study reach during all seasons.

Stream / Watershed Type / Reach Type / Season / k20.cm.hr / GPP / ER / NEP
Ashby Fk / Reference / Open / Fall / 77.7 / 5.5 / 5.7 / -0.2
Ashby Fk / Reference / Canopied / Fall / 82.0 / 6.7 / 9.9 / -3.2
Cruise Ck / Reference / Open / Fall / 88.0 / 2.6 / 6.3 / -3.6
Cruise Ck / Reference / Canopied / Fall / 55.3 / 2.3 / 7.1 / -4.8
Middle Ck / Reference / Open / Fall / 46.8 / 5.0 / 5.8 / -0.8
Middle Ck / Reference / Canopied / Fall / 42.5 / 4.7 / 9.0 / -4.3
Steep Ck / Reference / Open / Fall / 36.8 / 6.0 / 5.2 / 0.8
Steep Ck / Reference / Canopied / Fall / 39.7 / 5.6 / 7.7 / -2.1
Elijah / Urban / Open / Fall / 32.6 / 7.6 / 4.7 / 3.0
Elijah / Urban / Canopied / Fall / 15.7 / 1.0 / 2.4 / -1.4
Oakbrook / Urban / Open / Fall / 37.4 / 8.7 / 4.4 / 4.4
Oakbrook / Urban / Canopied / Fall / 5.6 / 1.3 / 2.4 / -1.1
Sunnybrook / Urban / Open / Fall / 41.7 / 7.0 / 5.6 / 1.3
Sunnybrook / Urban / Canopied / Fall / 16.8 / 1.6 / 2.1 / -0.5
Threemile / Urban / Open / Fall / 21.7 / 2.3 / 3.6 / -1.3
Threemile / Urban / Canopied / Fall / 22.6 / 1.9 / 4.6 / -2.7
Ashby Fk / Reference / Open / Spring / 40.9 / 12.6 / 5.3 / 7.3
Ashby Fk / Reference / Canopied / Spring / 32.3 / 8.7 / 4.7 / 4.0
Cruise Ck / Reference / Open / Spring / 39.1 / 8.1 / 11.3 / -3.3
Cruise Ck / Reference / Canopied / Spring / 58.6 / 9.8 / 9.5 / 0.3
Middle Ck / Reference / Open / Spring / 21.0 / 5.2 / 8.4 / -3.2
Middle Ck / Reference / Canopied / Spring / 16.0 / 4.0 / 6.3 / -2.2
Steep Ck / Reference / Open / Spring / 28.2 / 7.4 / 10.7 / -3.3
Steep Ck / Reference / Canopied / Spring / 34.4 / 6.8 / 9.8 / -3.0
Elijah / Urban / Open / Spring / 19.6 / 14.1 / 15.8 / -1.7
Elijah / Urban / Canopied / Spring / 26.7 / 14.7 / 19.7 / -5.0
Oakbrook / Urban / Open / Spring / 15.7 / 13.4 / 9.0 / 4.3
Oakbrook / Urban / Canopied / Spring / 20.9 / 16.0 / 13.0 / 3.0
Sunnybrook / Urban / Open / Spring / 7.6 / 6.1 / 5.0 / 1.1
Sunnybrook / Urban / Canopied / Spring / 22.5 / 13.4 / 12.3 / 1.1
Threemile / Urban / Open / Spring / 11.0 / 7.2 / 4.0 / 3.2
Threemile / Urban / Canopied / Spring / 15.4 / 10.0 / 4.9 / 5.1
Ashby Fk / Reference / Open / Summer / 6.0 / 3.3 / 6.9 / -3.6
Ashby Fk / Reference / Canopied / Summer / 10.4 / 6.9 / 17.4 / -10.5
Cruise Ck / Reference / Open / Summer / 22.2 / 8.6 / 19.8 / -11.2
Cruise Ck / Reference / Canopied / Summer / 5.1 / 0.0 / 1.4 / -1.4
Middle Ck / Reference / Open / Summer / 12.4 / 2.5 / 7.1 / -4.6
Middle Ck / Reference / Canopied / Summer / 34.5 / 3.0 / 13.2 / -10.1
Steep Ck / Reference / Open / Summer / 10.0 / 6.5 / 13.2 / -6.8
Steep Ck / Reference / Canopied / Summer / 5.6 / 1.0 / 5.8 / -4.8
Elijah / Urban / Open / Summer / 11.2 / 6.1 / 5.4 / 0.7
Elijah / Urban / Canopied / Summer / 16.2 / 9.2 / 8.7 / 0.4
Oakbrook / Urban / Open / Summer / 5.3 / 7.2 / 6.8 / 0.4
Oakbrook / Urban / Canopied / Summer / 7.9 / 5.4 / 5.8 / -0.3
Sunnybrook / Urban / Open / Summer / 6.7 / 9.7 / 8.1 / 1.7
Sunnybrook / Urban / Canopied / Summer / 8.1 / 2.6 / 3.8 / -1.2
Threemile / Urban / Open / Summer / 8.2 / 5.7 / 5.7 / 0.1
Threemile / Urban / Canopied / Summer / 8.3 / 4.2 / 5.7 / -1.5
Ashby Fk / Reference / Open / Winter / 35.5 / 2.0 / 1.2 / 0.8
Ashby Fk / Reference / Canopied / Winter / 23.5 / 1.0 / 0.9 / 0.1
Cruise Ck / Reference / Open / Winter / 46.8 / 4.9 / 3.6 / 1.3
Cruise Ck / Reference / Canopied / Winter / 42.0 / 4.8 / 3.8 / 1.0
Middle Ck / Reference / Open / Winter / 43.7 / 3.8 / 2.7 / 1.1
Middle Ck / Reference / Canopied / Winter / 32.8 / 3.5 / 3.8 / -0.3
Steep Ck / Reference / Open / Winter / 47.5 / 4.7 / 4.9 / -0.2
Steep Ck / Reference / Canopied / Winter / 41.8 / 4.7 / 4.0 / 0.7
Elijah / Urban / Open / Winter / 30.7 / 5.6 / 8.6 / -2.9
Elijah / Urban / Canopied / Winter / 20.7 / 5.7 / 9.7 / -4.0
Oakbrook / Urban / Open / Winter / 22.1 / 6.0 / 13.0 / -7.0
Oakbrook / Urban / Canopied / Winter / 21.8 / 6.4 / 11.9 / -5.5
Sunnybrook / Urban / Open / Winter / 24.8 / 5.0 / 9.3 / -4.3
Sunnybrook / Urban / Canopied / Winter / 31.8 / 5.2 / 8.8 / -3.6
Threemile / Urban / Open / Winter / 35.5 / 4.7 / 4.2 / 0.5
Threemile / Urban / Canopied / Winter / 28.8 / 3.8 / 3.4 / 0.3

Supplemental Table 2. Water chemistry at both base and elevated flows for each study reach during all seasons.

Stream / Watershed Type / Reach Type / Season / Flow (Base or Elevated) / pH / Cond / NH4 mg/L / PO4 mg/L / NO3 mg/L
Ashby Fk / Reference / Open / Fall / Base / 8.42 / 633 / 0.00 / 0.05 / 0.05
Ashby Fk / Reference / Canopied / Fall / Base / 8.31 / 628 / 0.01 / 0.03 / 0.06
Cruise Ck / Reference / Open / Fall / Base / 8.47 / 583 / 0.02 / 0.02 / 0.05
Cruise Ck / Reference / Canopied / Fall / Base / 8.44 / 582 / 0.03 / 0.04 / 0.06
Middle Ck / Reference / Open / Fall / Base / 8.65 / 541 / 0.01 / 0.02 / 0.08
Middle Ck / Reference / Canopied / Fall / Base / 8.62 / 545 / 0.02 / 0.07 / 0.07
Steep Ck / Reference / Open / Fall / Base / 8.12 / 551 / 0.02 / 0.06 / 0.07
Steep Ck / Reference / Canopied / Fall / Base / 8.21 / 556 / 0.03 / 0.13 / 0.09
Elijah / Urban / Open / Fall / Base / 8.6 / 966 / 0.04 / 0.13 / 0.10
Elijah / Urban / Canopied / Fall / Base / 8.49 / 945 / 0.02 / 0.13 / 0.09
Oakbrook / Urban / Open / Fall / Base / 8.36 / 812 / 0.03 / 0.13 / 0.09
Oakbrook / Urban / Canopied / Fall / Base / 8.29 / 831 / 0.03 / 0.13 / 0.09
Sunnybrook / Urban / Open / Fall / Base / 7.78 / 811 / 0.03 / 0.04 / 0.14
Sunnybrook / Urban / Canopied / Fall / Base / 7.83 / 812 / 0.02 / 0.04 / 0.14
Threemile / Urban / Open / Fall / Base / 8.06 / 912 / 0.02 / 0.04 / 0.13
Threemile / Urban / Canopied / Fall / Base / 8.31 / 921 / 0.03 / 0.04 / 0.14
Ashby Fk / Reference / Open / Spring / Base / 8.52 / 549 / 0.01 / 0.06 / 0.34
Ashby Fk / Reference / Canopied / Spring / Base / 8.26 / 546 / 0.02 / 0.08 / 0.42
Cruise Ck / Reference / Open / Spring / Base / 8.83 / 461 / 0.04 / 0.09 / 0.69
Cruise Ck / Reference / Canopied / Spring / Base / 8.92 / 465 / 0.10 / 0.12 / 0.66
Middle Ck / Reference / Open / Spring / Base / 8.19 / 487 / 0.11 / 0.13 / 0.52
Middle Ck / Reference / Canopied / Spring / Base / 8.49 / 450 / 0.07 / 0.16 / 0.51
Steep Ck / Reference / Open / Spring / Base / 8.77 / 480 / 0.08 / 0.18 / 0.33
Steep Ck / Reference / Canopied / Spring / Base / 8.78 / 471 / 0.11 / 0.16 / 0.37
Elijah / Urban / Open / Spring / Base / 8.57 / 889 / 0.12 / 0.07 / 0.45
Elijah / Urban / Canopied / Spring / Base / 8.5 / 886 / 0.34 / 0.12 / 0.58
Oakbrook / Urban / Open / Spring / Base / 8.45 / 980 / 0.35 / 0.13 / 0.58
Oakbrook / Urban / Canopied / Spring / Base / 8.41 / 940 / 0.05 / 0.11 / 0.58
Sunnybrook / Urban / Open / Spring / Base / 8.53 / 1143 / 0.06 / 0.10 / 0.50
Sunnybrook / Urban / Canopied / Spring / Base / 8.59 / 1054 / 0.07 / 0.12 / 0.52
Threemile / Urban / Open / Spring / Base / 8.7 / 1233 / 0.09 / 0.12 / 0.18
Threemile / Urban / Canopied / Spring / Base / 8.67 / 1240 / 0.06 / 0.10 / 0.12
Ashby Fk / Reference / Open / Summer / Base / 7.82 / 551 / 0.02 / 0.01 / 0.12
Ashby Fk / Reference / Canopied / Summer / Base / 7.21 / 566 / 0.00 / 0.02 / 0.08
Cruise Ck / Reference / Open / Summer / Base / 7.91 / 473 / 0.01 / 0.05 / 0.07
Cruise Ck / Reference / Canopied / Summer / Base / 7.78 / 479 / 0.01 / 0.06 / 0.04
Middle Ck / Reference / Open / Summer / Base / 7.73 / 499 / 0.11 / 0.03 / 0.02
Middle Ck / Reference / Canopied / Summer / Base / 7.7 / 498 / 0.11 / 0.02 / 0.05
Steep Ck / Reference / Open / Summer / Base / 7.6 / 502 / 0.00 / 0.07 / 0.07
Steep Ck / Reference / Canopied / Summer / Base / 7.59 / 494 / 0.01 / 0.08 / 0.16
Elijah / Urban / Open / Summer / Base / 7.99 / 854 / 0.05 / 0.11 / 0.14
Elijah / Urban / Canopied / Summer / Base / 8.02 / 819 / 0.06 / 0.13 / 0.12
Oakbrook / Urban / Open / Summer / Base / 8.11 / 881 / 0.11 / 0.09 / 0.09
Oakbrook / Urban / Canopied / Summer / Base / 8.12 / 872 / 0.12 / 0.09 / 0.08
Sunnybrook / Urban / Open / Summer / Base / 7.7 / 880 / 0.13 / 0.15 / 0.05
Sunnybrook / Urban / Canopied / Summer / Base / 7.83 / 881 / 0.14 / 0.16 / 0.05
Threemile / Urban / Open / Summer / Base / 8.13 / 920 / 0.11 / 0.13 / 0.04
Threemile / Urban / Canopied / Summer / Base / 8.02 / 926 / 0.11 / 0.16 / 0.05
Ashby Fk / Reference / Open / Winter / Base / 8.2 / 537 / 0.04 / 0.08 / 0.21
Ashby Fk / Reference / Canopied / Winter / Base / 8.14 / 521 / 0.03 / 0.09 / 0.22
Cruise Ck / Reference / Open / Winter / Base / 8.21 / 684 / 0.03 / 0.09 / 0.26
Cruise Ck / Reference / Canopied / Winter / Base / 8.18 / 638 / 0.04 / 0.05 / 0.31
Middle Ck / Reference / Open / Winter / Base / 8.19 / 507 / 0.02 / 0.04 / 0.34
Middle Ck / Reference / Canopied / Winter / Base / 8.16 / 512 / 0.04 / 0.07 / 0.33
Steep Ck / Reference / Open / Winter / Base / 7.98 / 612 / 0.03 / 0.03 / 0.45
Steep Ck / Reference / Canopied / Winter / Base / 7.86 / 621 / 0.03 / 0.02 / 0.37
Elijah / Urban / Open / Winter / Base / 8.12 / 1388 / 0.04 / 0.03 / 0.43
Elijah / Urban / Canopied / Winter / Base / 8.06 / 1371 / 0.04 / 0.02 / 0.47
Oakbrook / Urban / Open / Winter / Base / 8.24 / 1069 / 0.04 / 0.03 / 0.35
Oakbrook / Urban / Canopied / Winter / Base / 8.18 / 1038 / 0.03 / 0.02 / 0.32
Sunnybrook / Urban / Open / Winter / Base / 7.15 / 1096 / 0.01 / 0.01 / 0.39
Sunnybrook / Urban / Canopied / Winter / Base / 7.32 / 1122 / 0.04 / 0.01 / 0.36
Threemile / Urban / Open / Winter / Base / 8.5 / 1663 / 0.07 / 0.04 / 0.38
Threemile / Urban / Canopied / Winter / Base / 8.48 / 1672 / 0.01 / 0.01 / 0.39
Ashby Fk / Reference / Open / Fall / Elevated / 7.85 / 416 / 0.02 / 0.03 / 0.15
Ashby Fk / Reference / Canopied / Fall / Elevated / 7.68 / 422 / 0.04 / 0.02 / 0.11
Cruise Ck / Reference / Open / Fall / Elevated / 7.21 / 316 / 0.04 / 0.13 / 0.23
Cruise Ck / Reference / Canopied / Fall / Elevated / 7.34 / 309 / 0.05 / 0.11 / 0.22
Middle Ck / Reference / Open / Fall / Elevated / 8.34 / 522 / 0.04 / 0.11 / 0.19
Middle Ck / Reference / Canopied / Fall / Elevated / 8.37 / 511 / 0.04 / 0.11 / 0.23
Steep Ck / Reference / Open / Fall / Elevated / 7.9 / 336 / 0.03 / 0.07 / 0.14
Steep Ck / Reference / Canopied / Fall / Elevated / 7.96 / 341 / 0.03 / 0.07 / 0.18
Elijah / Urban / Open / Fall / Elevated / 8.47 / 406 / 0.02 / 0.17 / 0.28
Elijah / Urban / Canopied / Fall / Elevated / 8.49 / 411 / 0.02 / 0.18 / 0.27
Oakbrook / Urban / Open / Fall / Elevated / 7.09 / 341 / 0.01 / 0.06 / 0.14
Oakbrook / Urban / Canopied / Fall / Elevated / 7.03 / 336 / 0.03 / 0.05 / 0.12
Sunnybrook / Urban / Open / Fall / Elevated / 7.61 / 502 / 0.03 / 0.29 / 0.13
Sunnybrook / Urban / Canopied / Fall / Elevated / 7.65 / 494 / 0.02 / 0.31 / 0.16
Threemile / Urban / Open / Fall / Elevated / 8.41 / 708 / 0.05 / 0.30 / 0.19
Threemile / Urban / Canopied / Fall / Elevated / 8.27 / 711 / 0.04 / 0.31 / 0.21
Ashby Fk / Reference / Open / Spring / Elevated / 8.87 / 203 / 0.04 / 0.03 / 0.29
Ashby Fk / Reference / Canopied / Spring / Elevated / 8.95 / 193 / 0.03 / 0.05 / 0.34
Cruise Ck / Reference / Open / Spring / Elevated / 9.3 / 163 / 0.01 / 0.04 / 0.21
Cruise Ck / Reference / Canopied / Spring / Elevated / 9.22 / 158 / 0.03 / 0.03 / 0.20
Middle Ck / Reference / Open / Spring / Elevated / 8.61 / 199 / 0.03 / 0.03 / 0.32
Middle Ck / Reference / Canopied / Spring / Elevated / 9.02 / 170 / 0.05 / 0.05 / 0.27
Steep Ck / Reference / Open / Spring / Elevated / 9.32 / 213 / 0.05 / 0.04 / 0.17
Steep Ck / Reference / Canopied / Spring / Elevated / 9.09 / 211 / 0.01 / 0.03 / 0.20
Elijah / Urban / Open / Spring / Elevated / 8.38 / 373 / 0.02 / 0.00 / 0.02
Elijah / Urban / Canopied / Spring / Elevated / 8.34 / 375 / 0.02 / 0.01 / 0.02
Oakbrook / Urban / Open / Spring / Elevated / 8.42 / 351 / 0.52 / 0.01 / 0.27
Oakbrook / Urban / Canopied / Spring / Elevated / 8.34 / 365 / 0.48 / 0.01 / 0.31
Sunnybrook / Urban / Open / Spring / Elevated / 8.65 / 565 / 0.05 / 0.03 / 0.37
Sunnybrook / Urban / Canopied / Spring / Elevated / 8.53 / 538 / 0.05 / 0.03 / 0.28
Threemile / Urban / Open / Spring / Elevated / 8.6 / 524 / 0.10 / 0.05 / 0.57
Threemile / Urban / Canopied / Spring / Elevated / 8.82 / 509 / 0.07 / 0.06 / 0.53
Ashby Fk / Reference / Open / Summer / Elevated / 8.29 / 587 / 0.06 / 0.14 / 0.16
Ashby Fk / Reference / Canopied / Summer / Elevated / 8.12 / 597 / 0.07 / 0.14 / 0.13
Cruise Ck / Reference / Open / Summer / Elevated / 8.23 / 399 / 0.08 / 0.09 / 0.14
Cruise Ck / Reference / Canopied / Summer / Elevated / 8.29 / 404 / 0.06 / 0.08 / 0.09
Middle Ck / Reference / Open / Summer / Elevated / 8.21 / 485 / 0.07 / 0.06 / 0.10
Middle Ck / Reference / Canopied / Summer / Elevated / 8.3 / 479 / 0.08 / 0.04 / 0.10
Steep Ck / Reference / Open / Summer / Elevated / 8.16 / 536 / 0.11 / 0.07 / 0.11
Steep Ck / Reference / Canopied / Summer / Elevated / 8.2 / 549 / 0.29 / 0.08 / 0.18
Elijah / Urban / Open / Summer / Elevated / 8.01 / 572 / 0.25 / 0.13 / 0.21
Elijah / Urban / Canopied / Summer / Elevated / 8.06 / 564 / 0.14 / 0.12 / 0.17
Oakbrook / Urban / Open / Summer / Elevated / 8.15 / 709 / 0.15 / 0.23 / 0.16
Oakbrook / Urban / Canopied / Summer / Elevated / 8.01 / 719 / 0.14 / 0.23 / 0.12
Sunnybrook / Urban / Open / Summer / Elevated / 8.08 / 738 / 0.12 / 0.32 / 1.16
Sunnybrook / Urban / Canopied / Summer / Elevated / 8.12 / 696 / 0.16 / 0.34 / 0.20
Threemile / Urban / Open / Summer / Elevated / 8.3 / 716 / 0.16 / 0.40 / 0.19
Threemile / Urban / Canopied / Summer / Elevated / 8.17 / 705 / 0.12 / 0.34 / 0.20
Ashby Fk / Reference / Open / Winter / Elevated / 8.44 / 402 / 0.10 / 0.04 / 0.15
Ashby Fk / Reference / Canopied / Winter / Elevated / 8.51 / 413 / 0.07 / 0.04 / 0.13
Cruise Ck / Reference / Open / Winter / Elevated / 8.09 / 439 / 0.07 / 0.03 / 0.49
Cruise Ck / Reference / Canopied / Winter / Elevated / 8.13 / 451 / 0.04 / 0.05 / 0.34
Middle Ck / Reference / Open / Winter / Elevated / 8.02 / 459 / 0.01 / 0.05 / 0.37
Middle Ck / Reference / Canopied / Winter / Elevated / 7.89 / 461 / 0.04 / 0.08 / 0.37
Steep Ck / Reference / Open / Winter / Elevated / 8.54 / 341 / 0.04 / 0.08 / 0.36
Steep Ck / Reference / Canopied / Winter / Elevated / 8.56 / 339 / 0.05 / 0.03 / 0.34
Elijah / Urban / Open / Winter / Elevated / 8.31 / 994 / 0.05 / 0.04 / 0.41
Elijah / Urban / Canopied / Winter / Elevated / 8.44 / 958 / 0.04 / 0.03 / 0.69
Oakbrook / Urban / Open / Winter / Elevated / 8.33 / 1002 / 0.07 / 0.02 / 0.65
Oakbrook / Urban / Canopied / Winter / Elevated / 8.49 / 997 / 0.07 / 0.01 / 0.52
Sunnybrook / Urban / Open / Winter / Elevated / 8.09 / 893 / 0.06 / 0.01 / 0.69
Sunnybrook / Urban / Canopied / Winter / Elevated / 7.99 / 860 / 0.07 / 0.01 / 0.58
Threemile / Urban / Open / Winter / Elevated / 8.13 / 907 / 0.18 / 0.08 / 0.57
Threemile / Urban / Canopied / Winter / Elevated / 8.22 / 913 / 0.17 / 0.09 / 0.58