SupplementaryFigure S1Secondary structures of potential miRNAs in Euphorbiaceousplants. The region of the mature miRNA sequence is shown in CAPITAL letters.
Structure 1: miR156Initial ΔG = -47.80 kcal/mol
10 20 30 40
U -| c cuu cu uau
GACAGAAGAGAGUGAGCACcauggu uuc gcaua c
cugucuucucucacucgugguaucg aag cguau a
a a^ u --- cu uuu
80 70 60 50
Structure 2: miR156aInitial ΔG = -47.80 kcal/mol
10 20 30 40
U -| c cuu cu uau
GACAGAAGAGAGUGAGCACcauggu uuc gcaua c
cugucuucucucacucgugguaucg aag cguau a
a a^ u --- cu uuu
80 70 60 50
Structure 3: miR156aInitial ΔG = -47.80 kcal/mol
10 20 30 40
u -| c cuu cu uau
GACAGAAGAGAGUGAGCACcauggu uuc gcaua c
cugucu ucucucacucgug guaucg aag cguau a
a a^ u --- cu uuu
80 70 60 50
Structure 4: miR156a Initial ΔG = -46.00 kcal/mol
10 20 30 40
-| c cuu cu uau
GACAGA AGAGAGUGAGCACcauggu uuc gcaua c
cugucu ucucucacucgug guaucg aag cguau a
a^ u --- cu uuu
80 70 60 50
Structure 5: miR156bInitial ΔG = -47.70 kcal/mol
10 20 30 40
u -| c cuu cu uau
GACAGA AGAGAGUGAGCACcauggu uuc gcaua c
cugucu ucucucacucgug guaucg aag cguau a
a a^ u --- cu uuu
80 70 60 50
Structure 6: miR156b Initial ΔG = -47.70 kcal/mol
10 20 30 40
u -| c cuu cu uau
GACAGA AGAGAGUGAGCACcauggu uuc gcaua c
cugucu ucucucacucgug guaucg aag cguau a
a a^ u --- cu uuu
80 70 60 50
Structure 7: miR156b Initial dG = -40.30 kcal/mol
10 20 30 40
u - u -| u ga a
UGACAGAAG AUAGAGAGCACaga ga uga uuug guug a
acugucuuc uaucucucguguuu cu acu aaac uaau a
- g c c^ u -- u
80 70 60 50
Structure 8: miR156b Initial dG = -40.20 kcal/mol
10 20 30 40
u - u -| u ga a
GACAGAAG AUAGAGAGCACaga ga uga uuug guug a
cugucuuc uaucucucguguuu cu acu aaac uaau a
a g c c^ u -- u
80 70 60 50
Structure 9: miR156b Initial dG = -33.9 kcal/mol
10 20 30
- u -| u ga a
CAGAAG AUAGAGAGCACAga ga uga uuug guug a
gucuuc uaucucucguguuu cu acu aaac uaau a
g c c^ u -- u
70 60 50 40
Structure 10: miR156c Initial dG = -47.80 kcal/mol
10 20 30 40
u -| c cuu cu uau
GACAGA AGAGAGUGAGCACcauggu uuc gcaua c
cugucu ucucucacucgug guaucg aag cguau a
a a^ u --- cu uuu
80 70 60 50
Structure 11: miR156c Initial dG = -41.5 kcal/mol
10 20 30 40
-| c cuu cu uau
CAGA AGAGAGUGAGCACcauggu uuc gcaua c
gucu ucucucacucgug guaucg aag cguau a
a^ u --- cu uuu
. 70 60 50
Structure 12: miR156c Initial dG = -40.3 kcal/mol
10 20 30 40
u - u -| u ga a
UGACAGAAG AUAGAGAGCACaga ga uga uuug guug a
acugucuuc uaucucucguguuu cu acu aaac uaau a
- g c c^ u -- u
80 70 60 50
Structure 13: miR156c Initial dG = -40.3 kcal/mol
10 20 30 40
U - u -| u ga a
UGACAGAAG AUAGAGAGCACaga ga uga uuug guug a
acugucuuc uaucucucguguuu cu acu aaac uaau a
- g c c^ u -- u
80 70 60 50
Structure 14: miR156d Initial dG = -47.8 kcal/mol
10 20 30 40
U -| c cuu cu uau
GACAGA AGAGAGUGAGCAC cauggu uuc gcaua c
cugucu ucucucacucgug guaucg aag cguau a
a a^ u --- cu uuu
80 70 60 50
Structure 15: miR156d Initial dG = -47.8 kcal/mol
10 20 30 40
U -| c cuu cu uau
GACAGA AGAGAGUGAGCAC cauggu uuc gcaua c
cugucu ucucucacucgug guaucg aag cguau a
a a^ u --- cu uuu
80 70 60 50
Structure 16: miR156d Initial dG = -40.3 kcal/mol
10 20 30 40
u - u -| u ga a
UGACAGAAG AUAGAGAGCACaga ga uga uuug guug a
acugucuuc uaucucucguguuu cu acu aaac uaau a
- g c c^ u -- u
80 70 60 50
Structure 17: miR156e Initial dG = -47.8 kcal/mol
10 20 30 40
U -| c cuu cu uau
GACAGA AGAGAGUGAGCAC cauggu uuc gcaua c
cugucu ucucucacucgug guaucg aag cguau a
a a^ u --- cu uuu
80 70 60 50
Structure 18: miR156e Initial dG = -40.3 kcal/mol
10 20 30 40
U - u -| u ga a
UGACAGAAG AUAGAGAGCACaga ga uga uuug guug a
acugucuuc uaucucucguguuu cu acu aaac uaau a
- g c c^ u -- u
80 70 60 50
Structure 19: miR156f Initial dG = -47.8 kcal/mol
10 20 30 40
U -| c cuu cu uau
GACAGA AGAGAGUGAGCAC cauggu uuc gcaua c
cugucu ucucucacucgug guaucg aag cguau a
a a^ u --- cu uuu
80 70 60 50
Structure 20: miR156f Initial dG = -40.3 kcal/mol
10 20 30 40
U - u -| u ga a
UGACAGAAG AUAGAGAGCACaga ga uga uuug guug a
acugucuuc uaucucucguguuu cu acu aaac uaau a
- g c c^ u -- u
80 70 60 50
Structure 21: miR156g Initial dG = -46.1 kcal/mol
10 20 30 40
-| c cuu cu uau
GACAGA AGAGAGUGAGCAC cauggu uuc gcaua c
cugucu ucucucacucgug guaucg aag cguau a
a^ u --- cu uuu
80 70 60 50
Structure 22: miR156g Initial dG = -40.3 kcal/mol
10 20 30 40
U - u -| u ga a
UGACAGAAG AUAGAGAGCACaga ga uga uuug guug a
acugucuuc uaucucucguguuu cu acu aaac uaau a
- g c c^ u -- u
80 70 60 50
Structure 23: miR156h Initial dG = -40.3 kcal/mol
10 20 30 40
U - u -| u ga a
UGACAGAAG AUAGAGAGCACaga ga uga uuug guug a
acugucuuc uaucucucguguuu cu acu aaac uaau a
- g c c^ u -- u
80 70 60 50
Structure 24: miR156h Initial dG = -40.3 kcal/mol
10 20 30 40
U - u -| u ga a
UGACAGAAG AUAGAGAGCACaga ga uga uuug guug a
acugucuuc uaucucucguguuu cu acu aaac uaau a
- g c c^ u -- u
80 70 60 50
Structure 25: miR156i Initial dG = -40.2 kcal/mol
10 20 30 40
U - u -| u ga a
GACAGAAGAUAGAGAGCACaga ga uga uuug guug a
cugucuuc uaucucucguguuu cu acu aaac uaau a
a g c c^ u -- u
80 70 60 50
Structure 26: miR156j Initial dG = -47.8 kcal/mol
10 20 30 40
U -| c cuu cu uau
GACAGA AGAGAGUGAGCAC cauggu uuc gcaua c
cugucu ucucucacucgug guaucg aag cguau a
a a^ u --- cu uuu
80 70 60 50
Structure 27: miR156j Initial dG = -40.3 kcal/mol
10 20 30 40
U - u -| u ga a
UGACAGAAG AUAGAGAGCACaga ga uga uuug guug a
acugucuuc uaucucucguguuu cu acu aaac uaau a
- g c c^ u -- u
80 70 60 50
Structure 28: miR156k Initial dG = -47.8 kcal/mol
10 20 30 40
U -| c cuu cu uau
GACAGA AGAGAGUGAGCAC cauggu uuc gcaua c
cugucu ucucucacucgug guaucg aag cguau a
a a^ u --- cu uuu
80 70 60 50
Structure 29: miR157a Initial dG = -40.3 kcal/mol
10 20 30 40
U - u -| u ga a
UGACAGAAG AUAGAGAGCACaga ga uga uuug guug a
acugucuuc uaucucucguguuu cu acu aaac uaau a
- g c c^ u -- u
80 70 60 50
Structure 30: miR157b Initial dG = -40.3 kcal/mol
10 20 30 40
U - u -| u ga a
UGACAGAAG AUAGAGAGCACaga ga uga uuug guug a
acugucuuc uaucucucguguuu cu acu aaac uaau a
- g c c^ u -- u
80 70 60 50
Structure 31: miR157c Initial dG = -40.3 kcal/mol
10 20 30 40
U - u -| u ga a
UGACAGAAGAUAGAGAGCACaga ga uga uuug guug a
acugucuuc uaucucucguguuu cu acu aaac uaau a
- g c c^ u -- u
80 70 60 50
Structure 32: miR157d Initial dG = -40.2 kcal/mol
10 20 30 40
U - u -| u ga a
GACAGAAGAUAGAGAGCACaga ga uga uuug guug a
cugucuuc uaucucucguguuu cu acu aaac uaau a
a g c c^ u -- u
80 70 60 50
Structure 33: miR159 Initial dG = -73.6 kcal/mol
10 20 30 40 50 60 70 80
ga ag uu- ---| uc g c gauu a u a a- ua
uggagcuccuu aguccaaa aggau gca ggguaaa aagcu cuaau uaug cc cag ccua cccu au \
AUCUCGAGGGA UUAGGUUU uucua ugu uccauuu uucga gauug auac gg guu ggau gggg ua a
AG cu uuu uuc^ u- g u guuc c c a gg uu
. 160 150 140 130 120 110 100 90
Structure 34: miR159 Initial dG = -72.6 kcal/mol
10 20 30 40 50 60 70 80
ga ag uu- ---| uc g c gauu a u a a- ua
ggagcuccuu aguccaaa aggau gca ggguaaa aagcu cuaau uaug cc cag ccua cccu au \
UCUCGAGGGA UUAGGUUU uucua ugu uccauuu uucga gauug auac gg guu ggau gggg ua a
AG cu uuu uuc^ u- g u guuc c c a gg uu
160 150 140 130 120 110 100 90
Structure 35: miR159 Initial dG = -71.0 kcal/mol
10 20 30 40 50 60 70 80
ga ag uu- ---| uc g c gauu a u a a- ua
gagcuccuu aguccaaa aggau gca ggguaaa aagcu cuaau uaug cc cag ccua cccu au \
CUCGAGGGA UUAGGUUu uucua ugu uccauuu uucga gauug auac gg guu ggau gggg ua a
AG cu uuu uuc^ u- g u guuc c c a gg uu
160 150 140 130 120 110 100 90
Structure 36: miR159 Initial dG = -76.4 kcal/mol
10 20 30 40 50 60 70 80
- u aa uu ---| auc g c gauc a u cuaa
gagcuccuuuc guccaaa gaggauc gcu ggguag aagcu cugga uaug cca aag ccuauccu \
CUCGAGGGAAG UAGGUUu uuucuag uga uccauu uucga gacuu auac ggu uuc ggauggga u
C U a- uu uuu^ au- g u guuc a - auuu
160 150 140 130 120 110 100 90
Structure 37: miR159 Initial dG = -75.6 kcal/mol
10 20 30 40 50 60 70 80
u aa uu ---| auc g c gauc a u cuaa
gagcuccuuuc guccaaa gaggauc gcu ggguag aagcu cugga uaug cca aag ccuauccu \
CUCGAGGGAAG UAGGUUU uuucuag uga uccauu uucga gacuu auac ggu uuc ggauggga u
U a- uu uuu^ au- g u guuc a - auuu
160 150 140 130 120 110 100 90
Structure 38: miR159 Initial dG = -73.9 kcal/mol
10 20 30 40 50 60 70 80
ga ag uuu ---| auc g c gauu a u a auu
uggagcuccuu aguccaaa aggau gca ggguaa aagcu cugau uaug cc cag ccua cccua a
AUCUCGAGGGA UUAGGUUU uucua ugu uccauu uucga gauug auac gg guu ggau ggggu a
AG cu uuu uuc^ au- g u guuc c c a auu
. 160 150 140 130 120 110 100 90
Structure 39: miR159 Initial dG = -72.9 kcal/mol
10 20 30 40 50 60 70 80
ga ag uuu ---| auc g c gauu a u a auu
ggagcuccuu aguccaaa aggau gca ggguaa aagcu cugau uaug cc cag ccua cccua a
UCUCGAGGGA UUAGGUUU uucua ugu uccauu uucga gauug auac gg guu ggau ggggu a
AG cu uuu uuc^ au- g u guuc c c a auu
160 150 140 130 120 110 100 90
Structure 40: miR159 Initial dG = -71.9 kcal/mol
10 20 30 40 50 60 70 80
ga ag uuu ---| auc g c gauu a u a auu
gagcuccuu aguccaaa aggau gca ggguaa aagcu cugau uaug cc cag ccua cccua a
CUCGAGGGA UUAGGUUu uucua ugu uccauu uucga gauug auac gg guu ggau ggggu a
AG cu uuu uuc^ au- g u guuc c c a auu
160 150 140 130 120 110 100 90
Structure 41: miR159a Initial dG = -73.6 kcal/mol
10 20 30 40 50 60 70 80
ga ag uu- ---| uc g c gauu a u a a- ua
uggagcuccuu aguccaaa aggau gca ggguaaa aagcu cuaau uaug cc cag ccua cccu au \
AUCUCGAGGGA UUAGGUUU uucua ugu uccauuu uucga gauug auac gg guu ggau gggg ua a
AG cu uuu uuc^ u- g u guuc c c a gg uu
. 160 150 140 130 120 110 100 90
Structure 42: miR159a Initial dG = -72.6 kcal/mol
10 20 30 40 50 60 70 80
ga ag uu- ---| uc g c gauu a u a a- ua
ggagcuccuu aguccaaa aggau gca ggguaaa aagcu cuaau uaug cc cag ccua cccu au \
UCUCGAGGGA UUAGGUUU uucua ugu uccauuu uucga gauug auac gg guu ggau gggg ua a
AG cu uuu uuc^ u- g u guuc c c a gg uu
160 150 140 130 120 110 100 90
Structure 43: miR159a Initial dG = -72.6 kcal/mol
10 20 30 40 50 60 70 80
ga ag uu- ---| uc g c gauu a u a a- ua
ggagcuccuu aguccaaa aggau gca ggguaaa aagcu cuaau uaug cc cag ccua cccu au \
UCUCGAGGGA UUAGGUUU uucua ugu uccauuu uucga gauug auac gg guu ggau gggg ua a
AG cu uuu uuc^ u- g u guuc c c a gg uu
160 150 140 130 120 110 100 90
Structure 44: miR159a Initial dG = -71.0 kcal/mol
10 20 30 40 50 60 70 80
ga ag uu- ---| uc g c gauu a u a a- ua
gagcuccuu aguccaaa aggau gca ggguaaa aagcu cuaau uaug cc cag ccua cccu au \
CUCGAGGGA UUAGGUUu uucua ugu uccauuu uucga gauug auac gg guu ggau gggg ua a
AG cu uuu uuc^ u- g u guuc c c a gg uu
160 150 140 130 120 110 100 90
Structure 45: miR159a Initial dG = -76.4 kcal/mol
10 20 30 40 50 60 70 80
uu u aa uu ---| auc g c gauc a u cuaa
gagcuccuuuc guccaaa gaggauc gcu ggguag aagcu cugga uaug cca aag ccuauccu \
CUCGAGGGAAG UAGGUUu uuucuag uga uccauu uucga gacuu auac ggu uuc ggauggga u
AC U a- uu uuu^ au- g u guuc a - auuu
160 150 140 130 120 110 100 90
Structure 46: miR159a Initial dG = -75.6 kcal/mol
10 20 30 40 50 60 70 80
u aa uu ---| auc g c gauc a u cuaa
gagcuccuuuc guccaaa gaggauc gcu ggguag aagcu cugga uaug cca aag ccuauccu \
CUCGAGGGAAG UAGGUUU uuucuag uga uccauu uucga gacuu auac ggu uuc ggauggga u
U a- uu uuu^ au- g u guuc a - auuu
160 150 140 130 120 110 100 90
Structure 47: miR159a Initial dG = -75.6 kcal/mol
10 20 30 40 50 60 70 80
u aa uu ---| auc g c gauc a u cuaa
gagcuccuuuc guccaaa gaggauc gcu ggguag aagcu cugga uaug cca aag ccuauccu \
CUCGAGGGAAG UAGGUUU uuucuag uga uccauu uucga gacuu auac ggu uuc ggauggga u
U a- uu uuu^ au- g u guuc a - auuu
160 150 140 130 120 110 100 90
Structure 48: miR159a Initial dG = -72.9 kcal/mol
10 20 30 40 50 60 70 80
ga ag uuu ---| auc g c gauu a u a auu
ggagcuccuu aguccaaa aggau gca ggguaa aagcu cugau uaug cc cag ccua cccua a
UCUCGAGGGA UUAGGUUU uucua ugu uccauu uucga gauug auac gg guu ggau ggggu a
AG cu uuu uuc^ au- g u guuc c c a auu
160 150 140 130 120 110 100 90
Structure 49: miR159a Initial dG = -72.9 kcal/mol
10 20 30 40 50 60 70 80
ga ag uuu ---| auc g c gauu a u a auu
ggagcuccuu aguccaaa aggau gca ggguaa aagcu cugau uaug cc cag ccua cccua a
UCUCGAGGGA UUAGGUUu uucua ugu uccauu uucga gauug auac gg guu ggau ggggu a
AG cu uuu uuc^ au- g u guuc c c a auu
160 150 140 130 120 110 100 90
Structure 50: miR159a Initial dG = -71.3 kcal/mol
10 20 30 40 50 60 70 80
ga ag uuu ---| auc g c gauu a u a auu
gagcuccuu aguccaaa aggau gca ggguaa aagcu cugau uaug cc cag ccua cccua a
CUCGAGGGA UUAGGUUu uucua ugu uccauu uucga gauug auac gg guu ggau ggggu a
AG cu uuu uuc^ au- g u guuc c c a auu
160 150 140 130 120 110 100 90
Structure 51: miR159b Initial dG = -71.3 kcal/mol
10 20 30 40 50 60 70 80
ga ag uu- ---| uc g c gauu a u a a- ua
uggagcuccuu aguccaaa aggau gca ggguaaa aagcu cuaau uaug cc cag ccua cccu au \
AUCUCGAGGGA UUAGGUUU uucua ugu uccauuu uucga gauug auac gg guu ggau gggg ua a
AG cu uuu uuc^ u- g u guuc c c a gg uu
. 160 150 140 130 120 110 100 90
Structure 52: miR159b Initial dG = -72.6 kcal/mol
10 20 30 40 50 60 70 80
ga ag uu- ---| uc g c gauu a u a a- ua
ggagcuccuu aguccaaa aggau gca ggguaaa aagcu cuaau uaug cc cag ccua cccu au \
UCUCGAGGGA UUAGGUUU uucua ugu uccauuu uucga gauug auac gg guu ggau gggg ua a
AG cu uuu uuc^ u- g u guuc c c a gg uu
160 150 140 130 120 110 100 90
Structure 53: miR159b Initial dG = -72.6 kcal/mol
10 20 30 40 50 60 70 80
ga ag uu- ---| uc g c gauu a u a a- ua
ggagcuccuu aguccaaa aggau gca ggguaaa aagcu cuaau uaug cc cag ccua cccu au \
UCUCGAGGGA UUAGGUUu uucua ugu uccauuu uucga gauug auac gg guu ggau gggg ua a
AG cu uuu uuc^ u- g u guuc c c a gg uu
160 150 140 130 120 110 100 90
Structure 54: miR159b Initial dG = -71.0 kcal/mol
10 20 30 40 50 60 70 80
ga ag uu- ---| uc g c gauu a u a a- ua
gagcuccuu aguccaaa aggau gca ggguaaa aagcu cuaau uaug cc cag ccua cccu au \
CUCGAGGGA UUAGGUUu uucua ugu uccauuu uucga gauug auac gg guu ggau gggg ua a
AG cu uuu uuc^ u- g u guuc c c a gg uu
160 150 140 130 120 110 100 90
Structure 55: miR159b Initial dG = -76.4 kcal/mol
10 20 30 40 50 60 70 80
uu u aa uu ---| auc g c gauc a u cuaa
gagcuccuuuc guccaaa gaggauc gcu ggguag aagcu cugga uaug cca aag ccuauccu \
CUCGAGGGAAG UAGGUUu uuucuag uga uccauu uucga gacuu auac ggu uuc ggauggga u
AC U a- uu uuu^ au- g u guuc a - auuu
160 150 140 130 120 110 100 90
Structure 56: miR159b Initial dG = -76.4 kcal/mol
10 20 30 40 50 60 70 80
- u aa uu ---| auc g c gauc a u cuaa
gagcuccuuuc guccaaa gaggauc gcu ggguag aagcu cugga uaug cca aag ccuauccu \
CUCGAGGGAAG UAGGUUu uuucuag uga uccauu uucga gacuu auac ggu uuc ggauggga u
C U a- uu uuu^ au- g u guuc a - auuu
160 150 140 130 120 110 100 90
Structure 57: miR159b Initial dG = -75.6kcal/mol
10 20 30 40 50 60 70 80
u aa uu ---| auc g c gauc a u cuaa
gagcuccuuuc guccaaa gaggauc gcu ggguag aagcu cugga uaug cca aag ccuauccu \
CUCGAGGGAAG UAGGUUU uuucuag uga uccauu uucga gacuu auac ggu uuc ggauggga u
U a- uu uuu^ au- g u guuc a - auuu
160 150 140 130 120 110 100 90
Structure 58: miR159b Initial dG = -73.9 kcal/mol
10 20 30 40 50 60 70 80
ga ag uuu ---| auc g c gauu a u a auu
uggagcuccuu aguccaaa aggau gca ggguaa aagcu cugau uaug cc cag ccua cccua a
AUCUCGAGGGA UUAGGUUU uucua ugu uccauu uucga gauug auac gg guu ggau ggggu a
AG cu uuu uuc^ au- g u guuc c c a auu
. 160 150 140 130 120 110 100 90