GENERAL eNZYMOLOGY

Course code: / 13.1-WB-BT1-En
Type of course: / obligatory
Language of instruction: / Polish/English
Director of course: / Dr. Eng. Andrzej Kasperski
Name of lecturer: / Dr. Eng. Andrzej Kasperski
Type of course / Numbrr of hours per semester / Number of hours per week / Semester / Course grade / ECTS points
FULL-TIME STUDIES / 4
Lecture / 20 / 1,33 / 4 / exam
Laboratory / 30 / 2 / 4 / credit with grade

AIM OF THE COURSE:

Gaining knowledge about the classification of enzymes. Understanding the mechanism of action of enzymes, the construction and purpose of the important parts of the enzyme molecule. Understanding the basics of enzyme kinetics and the importance of the parameters describing this kinetics. Familiarization with the most important pathways and mechanisms of their control. Understanding of the mechanisms of activity control of the enzyme, its activation and inhibition. Familiarization with types of specific inhibition. Familiarization with impact of different types of inhibition to change of enzyme kinetic parameters. Gaining knowledge to plan and carry out enzymatic tests.

PREREQUISITES:

Basic knowledge about the structure of proteins. Skill of using the computer and the Internet.

SCOPE:

Definition and general characteristics of the enzymes. Characteristics of the different classes of enzymes. The role and mechanism of enzyme action. The role of catalytic center and substrate pocket. EC enzyme codes. Fundamentals of enzyme kinetics. Analysis of reversible and irreversible enzyme reaction. The Michaelis-Menten equation. The basic parameters describing the kinetics of enzyme - the maximum rate of the enzymatic reaction and the Michaelis constant. Identification of enzymes and metabolic pathways. Characteristics of selected enzymes and metabolic pathways and their control. Energy states during the enzymatic reaction. Important coenzymes and their precursors. The Lineweaver-Burk formula - the purpose and significance of such formula conversion. Planning and carrying out enzymatic test. The calculation of the kinetic parameters from the data obtained from enzymatic test. The Eadie-Hofstee, Hanes-Woolf, Integral method plots. Activation and control of enzymatic activity. Allosteric enzymes. Allosteric reaction kinetics. Cascade-enzymatic reactions. Factors affecting enzyme activity. Characteristics of the inhibition effects. The enzymatic reaction with competitive, acompetitive, noncompetitive, mixed, substrate, product inhibition. Analysis of enzymatic inhibition kinetics.

METHODS OF EDUCATION:

Lectures with multimedia presentations. Laboratories: using online enzymatic databases and specialized software, virtual enzymatic tests, development of topic related to the enzymology.

LEARNING OUTCOMES:

Effect of the course / Symbol / Methods of verification / Type of course
Student understands the basics of enzymatic processes. / K1A_W81 / Exam
Current control / Lecture
Laboratory
Has knowledge of basic concepts and terminology used in enzymology and enzymatic kinetics. / K1A_W83 / Exam
Current control / Lecture
Laboratory
Has knowledge of basic software for enzymatic kinetic analysis. / K1A_W84 / Exam
Current control / Lecture
Laboratory
Applies basic computational methods and software in the field of enzymatic kinetics. / K1A_U23 / Exam
Current control / Lecture
Laboratory
Shows the ability to draw correct conclusions on the basis of data from the enzymatic tests. / K1A_U25 / Current control / Lecture
Laboratory
Is aware of the need for systematic review of scientific literature, updates knowledge and knows its practical application. / K1A_K04 / Current control / Lecture
Laboratory

Verification of learning outcomes and credit conditions:

Lecture - written exam consists of 10 questions. The correct answers to at least 6 questions (60%) are required to get the pass mark credit. Laboratory assessment is evaluated on the basis of development and presentation of topics related to the enzymology (30 minute presentation), for example characterization and identification of selected enzymes, and demonstrate the ability to perform virtual enzymatic tests.

STUDENT WORKLOAD:

Workload / FULL-TIME STUDIES
(in hours)
The contact hours / 55
The unassisted student work / 50
In all / 105
ECTS Points
Classes with the participation of academic teacher / 2
Classes without the participation of an academic teacher / 2
In all / 4

LITERATURE:

RECOMMENDED READING:

·  Berg J.M., Tymoczko J.L., Stryer L., Biochemistry: International Edition, 2011,

·  Murray R.K., Bender D.A., Botham K.M., Kennelly P.J., Rodwell V.W., Weil P.A., Harper's Illustrated Biochemistry, Twenty-Eighth Edition, McGraw-Hill Companie, 2009.

OPTIONAL READING:

·  Garret R.H, Grisham C.M., Biochemistry, Thomson Learning, 2012.

AUTHOR OF THE PROGRAM: Dr. Eng. Andrzej Kasperski