LOCUS Exported 3789 bp ds-DNA circular SYN 12-JUN-2014
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS pCMV-Flag-N
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3789)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Thursday, Jun 12, 2014 from SnapGene 2.4.0
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3789
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 27..330
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 331..534
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
intron 672..768
/note="SV40 intron"
/note="modified SV40 intron with splice donor and acceptor
sites"
CDS 832..855
/codon_start=1
/product="FLAG epitope tag, followed by an enterokinase
cleavage site"
/note="DYKDDDDK-tag (FLAG epitope)"
/translation="DYKDDDDK"
gap 883..892
/estimated_length="10"
misc_feature 883..892
/note="ORF insert"
polyA_signal 928..1062
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
primer_bind complement(1173..1189)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
rep_origin complement(1575..2163)
/direction=LEFT
/note="ori"
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2334..3194)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3195..3299)
/gene="bla"
/note="AmpR promoter"
primer_bind 3773..3789
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat ggactacaag
841 gacgacgatg acaagcttat ggccatggag gcccgaattc ggnnnnnnnn nntcgaccga
901 gatctctcga ggtaccgcgg ccgcggggat ccagacatga taagatacat tgatgagttt
961 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
1021 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
1081 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttcggatcc tctagagtcg
1141 atctgcaggc atgctagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
1201 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
1261 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
1321 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
1381 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
1441 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
1501 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
1561 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
1621 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
1681 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
1741 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
1801 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
1861 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
1921 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
1981 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
2041 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
2101 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
2161 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
2221 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
2281 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
2341 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
2401 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
2461 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
2521 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
2581 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
2641 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
2701 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
2761 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
2821 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
2881 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
2941 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
3001 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
3061 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
3121 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
3181 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
3241 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
3301 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
3361 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
3421 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
3481 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
3541 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
3601 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt
3661 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
3721 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
3781 acggccagt
//