Supplementary Tables S1-S4
Tanja Pyhäjärvi1*, Sonja T. Kujala1*§ and Outi Savolainen*§
Journal: Tree Genetics and Genomes
Manuscript title: Revisiting protein heterozygosity in plants—nucleotide diversity in allozyme coding genes of conifer Pinus sylvestris
Corresponding author: Tanja Pyhäjärvi, Department of Biology, P.O. Box 3000, 90014 University of Oulu, Phone: +358 8553 1793, Fax: +358 8 553 4112, email:
Table S1
Sampling, data and references for the studies used for investigating relationship between nucleotide diversity and allozyme diversity in 27 plant species.
Nucleotide diversitySpecies / TREE / Samplinga / θb / Arcsinc / Reference
Arabidopsis lyrata / NO / 77 loci, Russian Karhumäki population / 0.0071 / 0.08 / Ross-Ibarra et al. 2008
Arabidopsis thaliana / NO / 335 loci, 12 accessions / 0.0081 / 0.09 / Schmid et al. 2005
Capsicum, semiwild / NO / 3 loci, 58 Mexican accessions / 0.0026 / 0.05 / Aguilar-Meléndez et al. 2009
Glycine max / NO / 13 BAC clones and 15 SSR flanking regions, 25 genotypes / 0.0026 / 0.05 / Zhu et al. 2003
Helianthus annuus, wild / NO / 18 loci, 10 populations / 0.0252 / 0.16 / Strasburg and Rieseberg 2008
Hordeum spontaneum / NO / 5 loci, 34 accessions / 0.0167 / 0.13 / Caldwell et al. 2006
Leavenworthia stylosa / NO / 8 loci, combined from several studies / 0.0425 / 0.21 / Table 5 in Filatov and Charlesworth 1999
Lolium perenne / NO / 11 loci, 20 European genotypes / 0.0250 / 0.16 / Xing et al. 2007
Lycopersicon chmielewskii / NO / 14 loci, 1 southcentral Peru population / 0.0003 / 0.02 / Roselius, Stephan and Städler 2005
Lycopersicon pimpinellifolium / NO / 14 loci, 1 northern Peru population / 0.0016 / 0.04 / Roselius, Stephan and Städler 2005
Oryza rufipogon / NO / 111 STS regions, 21 Asian accessions / 0.0052 / 0.07 / Caicedo et al. 2007
Secale cereale / NO / 14 amplicons, 5 inbred lines / 0.0203 / 0.14 / Varshney et al. 2007
Sorghum bicolor, cultivated / NO / 155 loci, 17 accessions / 0.0038 / 0.06 / Hamblin et al. 2006
Zea mays spp. parviglumis / NO / 26 loci, 13 individuals rangewide / 0.0170 / 0.13 / Ross-Ibarra, Tenaillon and Gaut 2009
Persea americana / YES / 4 loci, 21 wild accessions / 0.0102 / 0.10 / Chen et al. 2008
Picea abies / YES / 22 loci, 7 populations / 0.0040 / 0.06 / Heuertz et al. 2006
Picea breweriana / YES / 10 loci, 4 populations from natural range / 0.0020 / 0.04 / Chen et al. 2009
Picea glauca / YES / 10 loci, seeds from seed orchard originating from Québec populations / 0.0091 / 0.10 / Chen et al. 2009
Picea mariana / YES / 10 loci, seeds from seed orchard originating from Québec populations / 0.0046 / 0.07 / Chen et al. 2009
Pinus densata / YES / 7 loci, 5 populations from Tibetan plateau / 0.0122 / 0.11 / Ma, Szmidt and Wang 2006
Pinus radiata / YES / 7 loci, breeding population derived from Año Nuevo and Monterey / 0.0008 / 0.03 / Pot et al. 2005
Pinus sylvestris / YES / 16 loci, Northern group / 0.0072 / 0.09 / Pyhäjärvi et al. 2007
Pinus tabuliformis / YES / 7 loci, 3 populations from China / 0.0119 / 0.11 / Ma, Szmidt and Wang 2006
Pinus taeda / YES / 19 loci, 32 genotypes from natural range / 0.0064 / 0.08 / Brown et al. 2004
Pinus yunnanensis / YES / 7 loci, 3 populations from China / 0.0095 / 0.10 / Ma, Szmidt and Wang 2006
Populus tremula / YES / 5 loci, 4 European populations / 0.0160 / 0.13 / Ingvarsson et al. 2005
Pseudotsuga menziesii / YES / 121 loci, 24 unrelated trees / 0.0076 / 0.09 / Eckert et al. 2009
Allozyme diversity
Species / TREE / Sampling / Hd / Arcsinc / Reference
Arabidopsis lyrata / NO / 10 loci, Russian Karhumäki population / 0.269 / 0.55 / van Treuren et al. 1997
Arabidopsis thaliana / NO / 17 loci, 16 British populations / 0.148 / 0.40 / Abbot and Gomes 1989
Capsicum, semiwild / NO / 20 loci, 42 Mexican accessions / 0.077 / 0.28 / Loaiza-Figueroa et al. 1989
Glycine max / NO / 13 loci, 1005 accessions / 0.198 / 0.46 / Griffin and Palmer 1995
Helianthus annuus, wild / NO / 20 loci, 30 accessions / 0.310 / 0.59 / Cronn et al 1997
Hordeum spontaneum / NO / 4 loci, 148 accessions from Israel and Turkey / 0.514 / 0.80 / Kahler and Allard 1981
Leavenworthia stylosa / NO / 5 loci, 3 populations / 0.524 / 0.81 / Charlesworth and Yang 1998
Lolium perenne / NO / 7 loci, 20 European populations / 0.353 / 0.64 / Ursla Fernando, Hayward and Kearsey 1997
Lycopersicon chmielewskii / NO / 14 loci, 8 accessions from Peru / 0.053 / 0.23 / Rick et al. 1976 as reported in Hamrick, Linhart and Mitton 1979
Lycopersicon pimpinellifolium / NO / 11 loci, 43 populations from Ecuador and Peru / 0.128 / 0.37 / Rick, Fobes and Holle 1977 as reported in Hamrick, Linhart and Mitton 1979
Oryza rufipogon / NO / 22 loci, 21 Chinese populations / 0.095 / 0.31 / Gao and Hong 2000
Secale cereale / NO / 8 loci, 8 varieties / 0.295 / 0.57 / Adam, Simonsen and Loeschcke 1987
Sorghum bicolor, cultivated / NO / 27 loci, 83 accessions / 0.093 / 0.31 / Morden, Doebley and Schertz 1989
Zea mays spp. parviglumis / NO / 21 loci, 18 populations / 0.311 / 0.59 / Doebley, Goodman and Stuber 1984 as reported in Doebley 1989
Persea americana / YES / 10 loci, 33 accessions / 0.195 / 0.46 / Torres et al. 1978 as reported in Hamrick, Linhart and Mitton 1979
Picea abies / YES / 22 loci, 70 populations / 0.121 / 0.36 / Lagercrantz and Ryman 1990
Picea breweriana / YES / 26 loci, 4 populations / 0.200 / 0.46 / Ledig, Hodgskiss and Johnson 2005
Picea glauca / YES / 18 loci, 7 populations / 0.296 / 0.58 / Godt et al. 2001
Picea mariana / YES / 13 loci, 5 natural populations in Québec / 0.301 / 0.58 / Isabel, Beaulieu and Bousquet 1995
Pinus densata / YES / 12 loci, 3 populations from China / 0.232 / 0.50 / Wang, Szmidt and Savolainen 2001
Pinus radiata / YES / 31 loci, Año Nuevo population / 0.088 / 0.30 / Moran, Bell and Eldridge 1988
Pinus sylvestris / YES / 10 loci, 4 Finnish populations / 0.340 / 0.62 / Karhu et al. 1996
Pinus tabuliformis / YES / 12 loci, 8 populations from China / 0.205 / 0.47 / Wang, Szmidt and Savolainen 2001
Pinus taeda / YES / 25 loci, 90 clones / 0.282 / 0.56 / Conkle 1981 as reported on Ledig 1998
Pinus yunnanensis / YES / 12 loci, 3 populations from China / 0.190 / 0.45 / Wang, Szmidt and Savolainen 2001
Populus tremula / YES / 28 loci, 18 individuals from Europe / 0.127 / 0.36 / Rajora and Dancik 1992
Pseudotsuga menziesii / YES / 20 loci, 104 locations / 0.182 / 0.44 / Li and Adams 1989
a Someof the studies have wider sampling than reported here. Subsets are used for comparison to approximately match sampling from nucleotide diversity and allozyme diversity studies.
bθ-estimate: π if it is reported, otherwise Watterson’s θW. Estimate based on silent sites (synonymous and non-coding) recorded if available. Overall θ-estimate for the total sample is reported unless results are from a single population
cArcsin of square root of θ-estimate or He
d Total heterozygosity for the sample (HT) reported unless data from only one population are reported (in which case He for a population reported)
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Table S2
Electrophoretic alleles and protein haplotypes of trees that were used to identify six allozyme coding loci of Pinus sylvestris.
Locus / Tree-megagametophytea / Electrophoretic allele / Protein haplotypeb6pgdB / AC1029-4 / 2 / GRTG
6pgdB / AC1029-5 / 1 / DKTA
6pgdB / AC2047-2 / 2 / GKTA
6pgdB / AC2047-3 / 1 / DKTA
6pgdB / AC2081-1 / 2 / GRTG
6pgdB / AC2081-4 / 1 / DKTA
6pgdB / AC1002-2 / 1 / DKSA
6pgdB / AC1011-1 / 2 / GKTA
6pgdB / AC1014-1 / 1 / DKTA
6pgdB / AC1022-4 / 1 / DKTA
6pgdB / AC2033-5 / 1 / DKTA
6pgdB / AC2101-5 / 2 / GKTA
6pgdB / AC3013-1 / 1 / DKSA
6pgdB / AC3063-3 / 1 / DKTA
6pgdB / Y3001-1 / 2 / GKTA
6pgdB / Z3001-2 / 1 / DKTA
6pgdB / AC1029-4 / 2 / GRTG
6pgdB / AC1029-5 / 1 / DKTA
6pgdB / AC2047-2 / 2 / GKTA
6pgdB / AC2047-3 / 1 / DKTA
gdh / AC2033-2 / 2 / H
gdh / AC2033-5 / 1 / D
gdh / AC2081-1 / 2 / H
gdh / AC2081-4 / 1 / D
gdh / AC3036-2 / 1 / D
gdh / AC3036-3 / 2 / H
gdh / AC1002-2 / 1 / D
gdh / AC1011-1 / 2 / H
gdh / AC1014-1 / 2 / H
gdh / AC1022-2 / 2 / H
gdh / AC2101-1 / 2 / H
gdh / Y3001-1 / 1 / D
gdh / Z3001-4 / 1 / D
gotC / AC2033-2 / 0 / LN
gotC / AC2033-5 / 1 / LD
gotC / Y3001-1 / 1 / LD
gotC / Y3001-4 / 0 / VN
gotC / Z3001-2 / 1 / LD
gotC / Z3001-3 / 0 / LN
gotC / AC1001-6 / 0 / LN
gotC / AC1002-1 / 0 / LN
gotC / AC1011-7 / 0 / LN
gotC / AC1014-8 / 1 / LD
gotC / AC1019-8 / 0 / LN
gotC / AC1029-8 / 1 / LD
gotC / AC1065-2 / 0 / LN
gotC / AC2047-2 / 0 / LN
gotC / AC2081-3 / 0 / LN
gotC / AC3063-1 / 0 / LN
mdhA / AC1011-7 / 2 / TNN
mdhA / AC1011-8 / 1 / TDN
mdhA / AC2101-1 / 2 / TNN
mdhA / AC2101-5 / 1 / TDN
mdhA / AC3013-2 / 2 / TNN
mdhA / AC3013-5 / 1 / TDN
mdhA / AC3036-2 / 1 / TDN
mdhA / AC3036-3 / 2 / TNN
mdhA / AC1001-3 / 2 / NNN
mdhA / AC1002-1 / 2 / TNN
mdhA / AC1014-1 / 2 / NNN
mdhA / AC1019-2 / 2 / TNN
mdhA / AC1022-3 / 2 / TNN
mdhA / AC1029-8 / 1 / TDN
mdhA / AC1043-4 / 2 / TNN
mdhA / AC2033-2 / 2 / TNT
mdhA / AC2081-1 / 2 / TNN
mdhA / AC3063-3 / 2 / TNT
mdhA / Z3001-3 / 2 / TNN
mdhB / AC1002-1 / 3 / LL
mdhB / AC1002-2 / 1 / LL
mdhB / AC1011-7 / 1 / LL
mdhB / AC1011-8 / 2 / RL
mdhB / AC1019-2 / 2 / RL
mdhB / AC1019-3 / 1 / RF
mdhB / AC1043-4 / 3 / LL
mdhB / AC1043-5 / 2 / RL
mdhB / AC2033-2 / 1 / RL
mdhB / AC2033-5 / 2 / RL
mdhB / AC3013-1 / 1 / RL
mdhB / AC3013-2 / 2 / RL
mdhB / AC1014-1 / 1 / RF
mdhB / Z3001-3 / 1 / LL
aco / AC1001-3 / 2 / TDMPTKSQ
aco / AC1001-5 / 1 / ADMPTQTQ
aco / AC1011-1 / 2 / ADMPMKSQ
aco / AC1011-4 / 3 / TDLRTKSQ
aco / AC1014-1 / 1 / ADMPTQTQ
aco / AC1014-4 / 2 / ADMPTKSQ
aco / AC1022-2 / 2 / TDLPTKSQ
aco / AC1022-3 / 3 / AGMPTKTQ
aco / AC1043-4 / 3 / AGMPTKTQ
aco / AC1043-5 / 2 / TDLPTKSQ
aco / AC2101-1 / 3 / AGMPTKTQ
aco / AC2101-5 / 2 / SDMPTKSQ
aco / Y3001-1 / 3 / ADMPTKTK
aco / Y3001-4 / 2 / TDMPTKSQ
aco / Z3001-2 / 3 / ADMPTKTK
aco / Z3001-4 / 2 / TDMPTKSQ
a Two megagametophytes from each tree that was heterozygous for particular loci are presented first, followed by trees that were homozygous for the electrophoretic locus. Each tree’s number and megagametophyte number are separated by a dash.
b Only variable amino acid sites are presented. The amino acid change segregating with electrophoretic allele is indicated by boldface.
Locus / Tree-megagametophytea / Electrophoretic allele / Protein haplotypeb6pgdB / AC1002-2 / 1 / DKSA
6pgdB / AC3013-1 / 1 / DKSA
6pgdB / AC1029-5 / 1 / DKTA
6pgdB / AC2047-3 / 1 / DKTA
6pgdB / AC2081-4 / 1 / DKTA
6pgdB / AC1014-1 / 1 / DKTA
6pgdB / AC1022-4 / 1 / DKTA
6pgdB / AC2033-5 / 1 / DKTA
6pgdB / AC3063-3 / 1 / DKTA
6pgdB / Z3001-2 / 1 / DKTA
6pgdB / AC1029-5 / 1 / DKTA
6pgdB / AC2047-3 / 1 / DKTA
6pgdB / AC1029-4 / 2 / GRTG
6pgdB / AC2081-1 / 2 / GRTG
6pgdB / AC1029-4 / 2 / GRTG
6pgdB / AC2047-2 / 2 / GKTA
6pgdB / AC1011-1 / 2 / GKTA
6pgdB / AC2101-5 / 2 / GKTA
6pgdB / Y3001-1 / 2 / GKTA
6pgdB / AC2047-2 / 2 / GKTA
gdh / AC2033-5 / 1 / D
gdh / AC2081-4 / 1 / D
gdh / AC3036-2 / 1 / D
gdh / AC1002-2 / 1 / D
gdh / Y3001-1 / 1 / D
gdh / Z3001-4 / 1 / D
gdh / AC2033-2 / 2 / H
gdh / AC2081-1 / 2 / H
gdh / AC3036-3 / 2 / H
gdh / AC1011-1 / 2 / H
gdh / AC1014-1 / 2 / H
gdh / AC1022-2 / 2 / H
gdh / AC2101-1 / 2 / H
gotC / AC2033-2 / 0 / LN
gotC / Z3001-3 / 0 / LN
gotC / AC1001-6 / 0 / LN
gotC / AC1002-1 / 0 / LN
gotC / AC1011-7 / 0 / LN
gotC / AC1019-8 / 0 / LN
gotC / AC1065-2 / 0 / LN
gotC / AC2047-2 / 0 / LN
gotC / AC2081-3 / 0 / LN
gotC / AC3063-1 / 0 / LN
gotC / Y3001-4 / 0 / VN
gotC / AC2033-5 / 1 / LD
gotC / Y3001-1 / 1 / LD
gotC / Z3001-2 / 1 / LD
gotC / AC1014-8 / 1 / LD
gotC / AC1029-8 / 1 / LD
mdhA / AC1011-8 / 1 / TDN
mdhA / AC2101-5 / 1 / TDN
mdhA / AC3013-5 / 1 / TDN
mdhA / AC3036-2 / 1 / TDN
mdhA / AC1029-8 / 1 / TDN
mdhA / AC1001-3 / 2 / NNN
mdhA / AC1014-1 / 2 / NNN
mdhA / AC1011-7 / 2 / TNN
mdhA / AC2101-1 / 2 / TNN
mdhA / AC3013-2 / 2 / TNN
mdhA / AC3036-3 / 2 / TNN
mdhA / AC1002-1 / 2 / TNN
mdhA / AC1019-2 / 2 / TNN
mdhA / AC1022-3 / 2 / TNN
mdhA / AC1043-4 / 2 / TNN
mdhA / AC2081-1 / 2 / TNN
mdhA / Z3001-3 / 2 / TNN
mdhA / AC2033-2 / 2 / TNT
mdhA / AC3063-3 / 2 / TNT
mdhB / AC1002-2 / 1 / LL
mdhB / AC1011-7 / 1 / LL
mdhB / Z3001-3 / 1 / LL
mdhB / AC1019-3 / 1 / RF
mdhB / AC1014-1 / 1 / RF
mdhB / AC2033-2 / 1 / RL
mdhB / AC3013-1 / 1 / RL
mdhB / AC1011-8 / 2 / RL
mdhB / AC1019-2 / 2 / RL
mdhB / AC1043-5 / 2 / RL
mdhB / AC2033-5 / 2 / RL
mdhB / AC3013-2 / 2 / RL
mdhB / AC1002-1 / 3 / LL
mdhB / AC1043-4 / 3 / LL
aco / AC1001-5 / 1 / ADMPTQTQ
aco / AC1014-1 / 1 / ADMPTQTQ
aco / AC1011-1 / 2 / ADMPMKSQ
aco / AC1014-4 / 2 / ADMPTKSQ
aco / AC2101-5 / 2 / SDMPTKSQ
aco / AC1022-2 / 2 / TDLPTKSQ
aco / AC1043-5 / 2 / TDLPTKSQ
aco / AC1001-3 / 2 / TDMPTKSQ
aco / Y3001-4 / 2 / TDMPTKSQ
aco / Z3001-4 / 2 / TDMPTKSQ
aco / Y3001-1 / 3 / ADMPTKTK
aco / Z3001-2 / 3 / ADMPTKTK
aco / AC1022-3 / 3 / AGMPTKTQ
aco / AC1043-4 / 3 / AGMPTKTQ
aco / AC2101-1 / 3 / AGMPTKTQ
aco / AC1011-4 / 3 / TDLRTKSQ
a Each tree’s number and megagametophyte number are separated by a dash.
b Only variable amino acid sites are presented. The amino acid change segregating with electrophoretic allele is indicated by boldface.
Table S3
Primers used for sequencing the allozyme coding loci.
Locus / pcr/seq / Forward-primer / Reverse-primer / [MgCl] / Ta6pgdB / pcr / 5’-GCCGAGGATCTCAAGAATC-3’ / 5’-ATAAGCAATTGATAGGGAAAACTT-3’ / 2.0 / 50
seq / 5’-GCACCAGGCTTTTGATACTTG-3’ / 5’-CCATCCCCCTTATCATCCTTG-3’
seq / 5’-GATGGGAGTCTCTGGCGGTGAGGAA-3’
aco f1 / pcr / 5’-TCCTCAAGTGGGGCTCAAC-3’ / 5’-CGACCTGTTAACCTCAAATACTGC-3’ / 2.0 / 61
seq / 5’-AAATGGGCCACCATTATACATC-3’ / 5’-ATGGATGCTGTCCACTTTATACC-3’
seq / 5’-TGATTTGAGCACAGTCCATGA-3’ / 5’-TTTCCAGATGTTTATTTGATAATACTG-3’
seq / 5’-AGACAAAGCATTCCCACACC-3’ / 5’-GCTGTGGCTCACCTCATTTT-3’
pcr / 5’-TGGAGTGACAGCTACGGACTTAGTT-3’ / 5’-CAGCCCCAAGCATGACATTA-3’ / 2.5 / 57
seq / 5’-TTGCAAAAACCAGTGCAGTC-3’ / 5’-AGTGAGTTGTTCAAATCAGTGTCC-3’
aco f2 / pcr / 5’-CTACATGTATTGGAAACTCGGGAGA-3’ / 5’-ATGTGGACCAGGAGGAGTCATT-3’ / 1.5 / 54
pcr / 5’-TCCTGTTCCAACTGGCACTTTGTAT-3’ / 5’-CAGGCTTTATCACATTATCCTCTTA-3 ’* / 2.0 / 53
seq / 5’-GTTTATTGTTTTCAACCAGGGTGTG-3’ / 5’-ATAGCCTCATCAAAAATGTCCC-3’
seq / 5’-ATAAACAATAGAAGAGAACGGGTCA-3’
gdh / pcr / 5’-TGTGACTACTCGCTCCTACACTCT-3’ / 5’-CTACAATCTTTCCACCCTTTTCAT-3’ * / 1.5 / 52
seq / 5’-ATTATCCCCCATTGCTTGTATTTTC-3’ / 5’-CCAAAACCCTGAAAAGGTCA-3’
seq / 5’-TGGGGTAGTGGTAGGATGAATGGTG-3’ / 5’-CTCACCATTCATCCTACCACTACCC-3’
seq / 5’-TTTCTCATAATAGGGAACCACTGTG-3’ / 5’-AACAAAAGAAGGGTGTAGCATCC-3’
seq / 5’-TCTAAGGTTGAGTTACTTGATGG-3’ / 5’-CAATACCACCCTTTGCTCCCCCATA-3’
pcr / 5’-CTGCAACTGGTAGAGGTGTTATG-3’ / 5’-GTGGTTGACATGGAGAAAAGTGTA-3’ / 2.0 / 59
seq / 5’-TTGCATAGGCTACTTTGTTGAGA-3’ / 5’-CCAAAACCTCTACAACTTCAACTGA-3’
seq / 5’-GATAGACTTGCCAGTTTGCCTTTTT-3’ / 5’-ACACAACAAAACTGGCAAGAAGCAT-3’
gotC / pcr / 5’-AAGATCCGAGCCCTGTG-3’ / 5’-ACCTTCCATCACCAAGTATCAG-3’ / 1.5 / 59
pcr / 5’-ACAAGGAGTATTTGCCCATAACTG-3’ / 5’-TTTGCATACGGGTTCCATTTAG-3’ / 2.0 / 57
pcr / 5’-GGCAATCATCCCAAGATCTTTACTC-3’ / 5’-GTTCAGGCCTTGCATGTTTTATTTT-3’ / 2.0 / 55
seq / 5’-GGGATTTGCCAGTGGAAGCCTTGAT-3’ / 5’-AGTACATTGGGCGAATAACC-3’
mdhA / pcr / 5’-GGTTATGCATTGGTGCCTATGATT-3’ / 5’-ACTTGACGTTGTAACCAGCATACTC-3’ / 2.0 / 60
seq / 5’-AATAGGGCTCTTGGACAGATTTTAG-3’ / 5’-ACGTCTGGATACTGGCTTGAAGAGT-3’
seq / 5’-TTGCACGAGATCCTACTTATTCTGT-3’ / 5’-ATGCCAGGTGGAACATCATAA-3’
mdhB f1 / pcr / 5’-GAGATCTGAACAAGGAGGAGAAGA-3’ / 5’-CTGCTCCACATGATAAAAACTGAG-3’ / 2.0 / 50
seq / 5’-CGGGAAAAGATTACCTGTGC-3’
pcr / 5’-GTATCCTGCAATCTCGAGAGAAAA-3’ / 5’-GAGACCAACCATCAAGAGACAAAC-3’ / 2.5 / 55
mdhB f2 / pcr / 5’-ATTTTTGCACTAGAGGACCTTTTT-3’ / 5’-GCTTTGGCAGCATAGAAAGTCT-3’ / 2.0 / 51
pcr / 5’-TGTTAATTCAACTGTACCCATTGC-3’ / 5’-TCAGAGCTTTGATATCCTCATCTG-3’ / 2.5 / 55
PCR-primers and internal sequencing primers indicated in second column. PCR-primers were also used in sequencing reactions, except when indicated with*. [MgCl]: magnesium concentration (mM), Ta: annealing temperature in degree Celsius.
Table S4
Silentsiteπ, KA/KS and heterozygosities (Hpe and He) of Pinus sylvestris allozyme coding loci used for regression analysis
Locus / πsil / KA/KS / Hpea / Heb6pgdB / 0.009 / 0.463 / 0.43 / 0.401
aco f1 / 0.008 / 0.111 / 0.278 / 0.138
aco f2 / 0.009 / 0.126 / 0.278 / 0.138
gdh / 0.003 / NA / 0.458 / 0.463
gotC / 0.010 / 0.020 / 0.408 / NA
mdhA / 0.008 / 0.013 / 0.056 / 0.076
mdhB f1 / 0.007 / 0.130 / NA / 0.468
mdhB f2 / 0.009 / 0.300 / NA / 0.468
Notethat same heterozygosities have been used for both fragments of aco and mdhB. NA: data was either not available or not possible to calculate.
a Predicted from the nucleotide data of this study
b He in natural seedlings (Muona O, Harju A, Kärkkäinen K. 1988. Genetic comparison of natural and nursery grown seedlings of Pinus sylvestris using allozymes. Scand J for Res 3:37-46.)