Suppl. Table 1. Details of Primers Designed for Validation by RT-Qpcr of Select Transcripts

Suppl. Table 1. Details of primers designed for validation by RT-qPCR of select transcripts up-regulated in diseased palms compared to healthy palms and comparison of fold changes in gene expression, determined by RT-qPCR, with RNA-Seq data

Sl. No. / Transcript id / Gene annotation / Forward primers (5′–3′) / Reverse primers (5′–3′) / Product size
(bp) / Annealing
temperature (oC) / RNA-seq / qRT-PCR
log2 fold change / p-value / log2 fold change / p-value
1. / c15534_g1_i1 / Transcriptional factor TINY / AGCCTAGCTCCTCAACTCTAA / GGTGGAATGGAGAGCATCAA / 134 / 62 / 6.50 / 0.00 / 1.50 / 0.00
2. / c15371_g1_i1 / bZIP family transcription factor / GAGAGTTAGGGTTAGGGTTTGG / CTTCTCTCTCTGCGTCCTTTC / 106 / 62 / 3.06 / 0.00 / 2.25 / 0.00
3. / c137567_g1_i1 / RING zinc finger protein-like / CCTTGGGAGGATTATGCATTCTA / CACCCGAAGCCTACTCTTTATC / 102 / 62 / 4.72 / 0.00 / 1.26 / 0.00
4. / c129423_g1_i1 / Immediate-early fungal elicitor protein / CATTCTCTGACCCAAGCTCTATT / TACAACCAAACTCACCACTCTAC / 147 / 62 / 3.61 / 0.00 / 2.13 / 0.00
5. / c17142_g1_i1 / Serine/threonine-protein kinase receptor / TTTTCCTCGAAATGGAGCTG / ACCTCCAAAGGAGGGAGAGA / 104 / 60 / 3.84 / 0.01 / 1.72 / 0.00
6. / c35923_g2_i1 / Myb-like HTH transcriptional regulator family protein / GTTCCCTGCTGCTTCTTGAC / TGGGCTTGTCCAAAGTCTTC / 132 / 60 / 2.18 / 0.00 / 2.19 / 0.00
7. / c22911_g1_i2 / Glutathione-S-transferase theta / GAGCTTTTCGCATCTTCCAA / TCTTTCTCACCTCCCCAATG / 134 / 60 / 4.19 / 0.00 / 2.0 / 0.00
8. / c45665_g1_i1 / CASP-like protein / CACCATCGAGTCTGCTCATATT / AGAGCTCCCGTATCGAGTT / 111 / 62 / 3.64 / 0.00 / 2.17 / 0.00
9. / c33721_g1_i2 / Class II Chitinase / CCTGGCAGGAACTACAACTATG / TTCCGTCATCCAGAACCATAAC / 129 / 62 / 3.84 / 0.00 / 1.97 / 0.00
10. / c16823_g1_i1 / Cyclic nucleotide-gated cation channel / CCTGTTCGAGAGAAGCCTGT / AGCATGAAAGGAGGTTTTGG / 108 / 59 / 3.93 / 0.00 / 1.98 / 0.00
11. / c64028_g1_i1 / WRKY DNA-binding protein / AATCAGCAGCAGCAGAGATAG / TCCATTGCTCTTCCACCTTAC / 120 / 62 / 2.03 / 0.01 / 1.72 / 0.00
12. / c9795_g1_i1 / AP2/ERF domain-containing transcription factor / CGCCGAAGTCCATACAGAAA / CCTCACTTTCCACAGCTCAA / 121 / 50 / 3.50 / 0.00 / 1.83 / 0.00
13. / c49946_g1_i1 / Cysteine-rich receptor-like protein kinase / GTGCTTCCATGCACAGCTTA / TGGGCACCTCTCAACAAAAT / 133 / 60 / 2.52 / 0.01 / 2.05 / 0.00
14. / c83981_g1_i1 / Major latex protein 22 (Pathogenesis-related protein 2) / CTTCAGGTGGTTCCGAAGAG / GCAGTCCCAAGGAGGAAGT / 107 / 59 / 3.19 / 0.00 / 1.99 / 0.00
15. / c43973_g2_i1 / Endo-1,3-1,4-β-D-glucanase / GCCCCATTGCTATACTTGGA / TCAAGCGTGTCTTGATGAGC / 208 / 59 / 2.63 / 0.00 / 1.31 / 0.00