Supplementary Data1 Evaluated Genes in the Fluid Micro Card Taqman Array Andbiological

Supplementary Data1– Evaluated genes in the fluid micro card TaqMan® Array andbiological process which they are involved.

Assay ID / Gene Symbol / Description / Gene name / Process*
Rn01436237_m1 / Abl1 / C-abl oncogene 1, receptor tyrosine kinase / Abl / 1
Rn00667869_m1 / Actb / Endogenous control / - / -
Rn00583646_m1 / Akt / v-akt murine thymoma viral oncogene homolog / - / 1,2
Rn00576832_m1 / Apaf1 / Apoptotic peptidase activatingfactor1 / - / 2, 5
Rn00821186_g1 / Apex1 / APEX nuclease (multifunctional DNA repair enzyme) 1 / APE, Apex, REF-1 / 3, 4, 5
Rn01513238_m1 / Api5 / Apoptosisinhibitor5 / MGC187857 / 2
Rn00565750_m1 / Ahr / Aryl hydrocarbon receptor / - / 7
Hs01005075_m1 / Ahrr / Aryl hydrocarbon receptor repressor / - / 7
Rn00572605_m1 / Atxn3 / Ataxin3 / Rsca3 / 4
Rn01515235_m1 / Aven / Apoptosis, caspaseactivationinhibitor / - / 2
Rn00575519_m1 / Bad / BCL2-associated agonist of cell death / MGC72439 / 2, 6
Rn01439834_m1 / Bag1 / BCL2-associated athanogene / - / 2, 5, 6
Rn02532082_g1 / Bax / Bcl2-associated X protein / - / 2, 5
Rn99999125_m1 / Bcl2 / B-cell CLL/lymphoma2 / Bcl-2 / 1, 2, 5, 6
Rn01459517_m1 / Bid / BH3 interacting domain death agonist / - / 2, 5
Rn00560871_m1 / Brca1 / Breastcancer1 / - / 1, 3, 4, 5
Rn00580597_m1 / Brca2 / Breastcancer2 / - / 1, 3, 4, 5
Rn01258147_m1 / Camk2a / Calcium/calmodulin-dependent protein kinase II alpha / PK2CDD, PKCCD / 1
Rn00563902_m1 / Casp3 / Caspase3 / Lice, MGC93645 / 1, 2
Rn00586960_m1 / Casp4 / Caspase4, apoptosis-relatedcysteine peptidase / Casp11, MGC124949 / 2
Rn00669368_g1 / Casp6 / Caspase6 / MGC93335, Mch2 / 2
Rn00573917_m1 / Casp7 / Caspase7 / - / 2
Rn00574069_m1 / Casp8 / Caspase8 / - / 2
Rn00581212_m1 / Casp9 / Caspase9, apoptosis-relatedcysteine peptidase / Apaf3, Casp-9-CTD, Casp9_v1, Ice-Lap6, Mch6 / 2, 5
Rn01493715_m1 / Ccna2 / Cyclin A2 / MGC156527 / 1, 6
Rn02346769_g1 / Ccnb2 / Cyclin B2 / MGC108931 / 1, 5
Rn00432360_m1 / Ccnd1 / Cyclin D1 / - / 1, 6
Rn01457762_m1 / Ccne1 / Cyclin E1 / CYCLE, Ccne / 1, 6
Rn01442731_m1 / Ccne2 / Cyclin E2 / - / 5, 6
Rn01423590_m1 / Cd40 / CD40 molecule, TNF receptor superfamily member 5 / Tnfrsf5 / 2
Rn00563924_m1 / Cd44 / Cd44 molecule / CD44A, METAA, MGC124941, RHAMM / 6
Rn00592073_m1 / Cdc25a / Cell division cycle 25 homolog A (S. pombe) / - / 1, 5
Rn00570728_m1 / Cdk1 / Cyclin-dependentkinase1 / Cdc2, Cdc2a / 5
Rn99999090_m1 / Cdk4 / Cyclin-dependentkinase4 / - / 1, 5
Rn01427989_s1 / Cdkn1a / Cyclin-dependent kinase inhibitor 1A / Cip1, Waf1 / 1, 5, 6
Rn00580664_m1 / Cdkn2a / Cyclin-dependent kinase inhibitor 2A / Arf, INK4A, MTS1, p16, p16Cdkn2a, p19ARF / 1, 5, 6
Rn04181355_m1 / Cidea / Cell death-inducing DFFA-like effector a / - / 2
Rn01489052_m1 / Cideb / Cell death-inducing DFFA-like effector b / - / 2
Rn01496517_g1 / Cldn7 / Claudin7 / MGC124679 / 6
Rn00594376_m1 / Dad1 / Defender againstcelldeath1 / - / 2
Rn01413725_m1 / Dclre1a / DNA cross-link repair 1A, PSO2 homolog (S. cerevisiae) / - / 3, 4
Rn00709938_m1 / Ddb1 / Damage-specific DNA binding protein 1 / - / 4
Rn00709664_m1 / Dnmt1 / DNA (cytosine-5-)-methyltransferase1 / - / 5
Rn01536222_m1 / E2f1 / E2F transcriptionfactor1 / - / 1, 5
Rn01434981_m1 / E2f3 / E2F transcriptionfactor3 / RGD1561600 / 1, 5
Rn00580398_m1 / Egfr / Epidermalgrowthfactor receptor / ERBB1, ErbB-1, Errp / 6
Rn00561138_m1 / Egr1 / Earlygrowth response 1 / Krox-24, NGFI-A, Ngf1, Ngfi, zif-268 / 5
Rn00591432_m1 / Ep300 / E1A bindingprotein p300 / - / 5
Rn00566561_m1 / Erbb2 / V-erb-b2 erythroblasticleukemia viral oncogenehomolog2, neuro/glioblastomaderivedoncogenehomolog (avian) / - / 6
Rn01498472_m1 / Ercc1 / Excision repair cross-complementing rodent repair deficiency, complementation group 1 / - / 3, 4, 5
Rn01307202_g1 / Ercc2 / Excision repair cross-complementing rodent repair deficiency, complementation group 2 / - / 3, 4
Rn01640372_m1 / Esr1 / Estrogen receptor 1 / ER-alpha, Esr, RNESTROR / 5, 6
Rn00562610_m1 / Esr2 / Estrogen receptor 2 (ER beta) / ER-beta, ERbeta, Erb2 / 6
Rn02103426_s1 / Exo1 / Exonuclease1 / - / 3, 4
Rn00584807_m1 / Fen1 / Flap structure-specificendonuclease1 / FEN-1, MGC94425 / 3, 4
Rn01441087_m1 / Foxo3 / Forkheadbox O3 / Fkhrl1, Foxo3a / 5
Rn01425130_g1 / Gadd45a / Growth arrest and DNA-damage-inducible, alpha / Ddit1, Gadd45 / 1, 2, 3, 5
Rn00565473_m1 / Igfbp2 / Insulin-like growth factor binding protein 2 / IGFBP-2, ILGFBPA / 6
Rn99999045_s1 / Jun / Junoncogene / - / 5, 6
Rn00590612_m1 / Lcn2 / Lipocalin2 / - / 6
Rn01403106_m1 / Map2k7 / Mitogen activated protein kinase kinase 7 / 2, JNKK / 6
Rn00587215_m1 / Mapk8ip1 / Mitogen-activated protein kinase 8 interacting protein 1 / JIP1, Jip-1, Mapk8ip / 2
Rn01459459_m1 / Mbd4 / Methyl-CpG binding domain protein 4 / - / 3
Rn00563462_m1 / Mgmt / O-6-methylguanine-DNA methyltransferase / - / 3, 4
Rn01451446_m1 / Mki67 / Antigen identified by monoclonal antibody Ki-67 / - / 1, 6
Rn00588658_g1 / Mt3 / Metallothionein3 / GIF, Mt-3 / 6
Rn00561507_m1 / Myc / Myelocytomatosisoncogene / MGC105490, RNCMYC, c-myc, mMyc / 5
Rn01757809_m1 / Naip2 / NLR family, apoptosis inhibitory protein 2 / Birc1, Birc1a, Birc1b, Naip / 2
Rn00561608_m1 / Nf1 / Neurofibromin1 / - / 5
Rn01399583_m1 / Nfkb1 / Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 / NF-kB / 2, 5
Rn01534371_m1 / Notch2 / Notchhomolog2 (Drosophila) / - / 1
Rn00565018_m1 / Parp1 / Poly (ADP-ribose) polymerase1 / Adprt, MGC93658, Parp-1 / 3, 4
Rn00567575_m1 / Polb / Polymerase (DNA directed), beta / - / 2, 4
Rn00572267_m1 / Pold1 / Polymerase (DNA directed), delta 1, catalytic subunit / - / 3
Rn01481627_m1 / Pole / Polymerase (DNA directed), epsilon / - / 3
Rn01494372_m1 / Polk / Polymerase (DNA directed) kappa / Dinb1 / 3
Rn00982780_m1 / Pum1 / Endogenous control / - / -
Rn01410979_m1 / Poll / Polymerase (DNA directed), lambda / - / 4
Rn01477771_m1 / Prc1 / Proteinregulatorofcytokinesis1 / - / 5
Rn01753308_m1 / Rb1 / Retinoblastoma1 / - / 5
Rn01502266_m1 / Rela / V-relreticuloendotheliosis viral oncogenehomolog A (avian) / NFkB / 5
Rn00821946_g1 / Rpl13a / Endogenous control / - / -
Rn01426378_m1 / Serpinb5 / Serpin peptidase inhibitor, clade B (ovalbumin), member 5 / Maspin, PI-5, Pi5 / 5, 6
Rn01402563_g1 / Skp2 / S-phase kinase-associated protein 2 (p45) / RGD1562456 / 1
Rn01457923_g1 / Sphk2 / Sphingosinekinase2 / - / 2
Rn01513693_m1 / Thbs1 / Thrombospondin1 / TSP-1, Tsp1 / 6
Rn02111874_s1 / Thbs2 / Thrombospondin2 / TSP-2 / 6
Rn00755717_m1 / Tp53 / Tumor protein p53 / MGC112612, Trp53, p53 / 1, 2, 3, 5, 6
Rn01404783_m1 / Tp63 / Tumor protein p63 / Ket, P73l, Tp73l, Trp63 / 1, 2, 5
Rn01520391_m1 / Tp73 / Tumor protein p73 / P73, Trp73 / 2
Rn01474701_m1 / Trfc / Endogenous control / - / -
Rn01511601_m1 / Vegfa / Vascular endothelial growth factor A / VEGF164, Vegf / 6
Rn00579738_m1 / Vim / Vimentin / - / 6
Rn00580566_m1 / Wt1 / Wilms tumor 1 / - / 5
Rn01462028_m1 / Zmat3 / Zincfinger, matrintype3 / PAG608, Wig1 / 5

*1- Cell cycle; 2- Apoptosis; 3- DNA damage; 4- DNA repair; 5- p53 gene cascade; 6- Estrogen receptor cascade; 7- Ahr activation. All genes were inventoried and. β-Actin, Gapdh, Gusb and Hprt1genes were the endogenous controls available in the assay.