LOCUS pMA1696 7585 bp DNA circular 20-OCT-2005
DEFINITION pBABE hygro+ NAP.
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
COMMENT VNTDATE|354896759|
COMMENT VNTDBDATE|388856502|
COMMENT LSOWNER|
COMMENT VNTNAME|pMA1696|
COMMENT VNTAUTHORNAME|Mikhail Alexeyev|
FEATURES Location/Qualifiers
promoter 1459..2094
/vntifkey="29"
/label=Pcmv-human
misc_signal 480..696
/vntifkey="87"
/label=Packaging\psi
promoter 3420..3760
/vntifkey="29"
/label=SV40\promoter-enhancer
LTR 4862..5455
/vntifkey="19"
/label=3'_LTR
CDS 536..1358
/vntifkey="4"
/label=GAG\trunc
LTR 19..479
/vntifkey="19"
/label=MoMuLV\LTR
CDS complement(6600..7457)
/vntifkey="4"
/label=Ap-res
CDS 3866..4819
/vntifkey="4"
/label=hph\coli\hygr-res
CDS 2202..3377
/codon_start=1
/product="nucleosome assembly protein"
/protein_id="AAC67388.1"
/db_xref="GI:3786398"
/vntifkey="4"
/label=NAP\rat\corrected
CDS 2157..2189
/vntifkey="4"
/label=HAtag\hum
RBS 2145..2150
/vntifkey="32"
/label=Kozak
BASE COUNT 1888 a 1929 c 1928 g 1840 t
ORIGIN
1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
541 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
961 ccttaagttt gaccttagat cactggaaag atgtcgagcg gctcgctcac aaccagtcgg
1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
1261 ctcttcttcc atccgcgccg tctctccccc ttgaacctcc tctttcgacc ccgcctcaat
1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatcaat tcgccagcac
1381 agtggtcgaa tcgaattcct gcagcccggg ggatccacta gttctagagc ggccgccacc
1441 gcggtggagc tcgaattgac gcgttgacat tgattattga ctagttatta atagtaatca
1501 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
1561 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
1621 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga ctatttacgg
1681 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
1741 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
1801 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
1861 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
1921 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
1981 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
2041 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
2101 actcactata gggagaccca agcttggtac cgagctcgga tctcgccacc atggcctacc
2161 cctacgacgt gcccgactac gccagcctgg gatccgccac catggcagac attgacaaca
2221 aagaacagtc tgaacttgat caagatttgg aagatgttga agaagtagaa gaagaagaaa
2281 cgggtgaaga aacaaaaatc aaagcacgtc agctaactgt tcagatgatg caaaatcctc
2341 agattcttgc agctcttcag gaaagacttg atggtctggt agacacacca acaggataca
2401 ttgaaagctt gcctaaggta gtcaaaagac gggtgaatgc tctcaagaat cttcaagtta
2461 aatgtgcaca gatagaagcc aaattctatg aggaagttca tgaccttgag agaaagtatg
2521 ctgttctcta tcagcctctg tttgataagc gatttgagat cattaatgca atttatgaac
2581 ctacagaaga agaatgtgaa tggaaaccag atgaggaaga tgaagtttcg gaggagctga
2641 aagaaaaggc caagattgaa gatgagaaaa aggatgaaga aaaagaagac cctaaaggaa
2701 ttcctgagtt ttggttgaca gtttttaaga atgttgattt gctcagtgat atggttcagg
2761 aacatgacga acctattctg aagcacttga aagatattaa agtgaagttt tcggacgctg
2821 gccagcctat gagttttatc ttagaatttc actttgaacc caacgaatat ttcacaaatg
2881 aagtgttaac aaagacttac aggatgaggt cagaaccaga tgattctgat cccttttctt
2941 ttgatggacc agaaattatg ggttgtacag ggtgccagat agattggaaa aaaggaaaga
3001 atgttacttt gaaaaccatt aagaagaagc agaaacacaa gggccgtggg acagttcgta
3061 ctgtgactaa aacagtttcc aatgattctt tctttaactt ttttgctcct cctgaagttc
3121 ctgagaacgg agatctggat gacgatgctg aggcaatact ggctgcagac tttgaaattg
3181 gtcacttttt acgtgagcgt ataatcccaa gatcagtgtt atacttcact ggagaagcta
3241 ttgaggacga tgacgatgac tatgatgaag aaggtgaaga agctgatgag gaaggggaag
3301 aagaaggaga tgaggaaaac gatccagact atgacccaaa gaaggatcaa aacccagccg
3361 agtgcaagca gcagtgagca gtgactcgag gtcgacggta tcgataagct tgattcgacc
3421 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
3481 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
3541 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
3601 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
3661 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
3721 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctagctta ccatgaccga
3781 gtacaagccc atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga
3841 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt
3901 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
3961 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt
4021 gcttgacatt ggggagttca gcgagagcct gacctattgc atctcccgcc gtgcacaggg
4081 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga
4141 ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg
4201 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc
4261 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc
4321 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
4381 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg
4441 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
4501 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc
4561 aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag
4621 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt
4681 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
4741 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
4801 gagggcaaag gaatatcgcg ataaaataaa agattttatt tagtctccag aaaaaggggg
4861 gaatgaaaga ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg
4921 catggaaaaa tacataactg agaatagaga agttcagatc aaggtcagga acagatggaa
4981 cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc
5041 caagaacaga tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct
5101 gccccggctc agggccaaga acagatggtc cccagatgcg gtccagccct cagcagtttc
5161 tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat
5221 ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc
5281 aataaaagag cccacaaccc ctcactcggg gcgccagtcc tccgattgac tgagtcgccc
5341 gggtacccgt gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt
5401 ccttgggagg gtctcctctg agtgattgac tacccgtcag cgggggtctt tcatttccga
5461 cttgtggtct cgctgccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg
5521 gtcttcacat gcagcatgta tcaaaattaa tttggttttt tttcttaagt atttacatta
5581 aatggccata gttgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
5641 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
5701 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
5761 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
5821 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
5881 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
5941 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
6001 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
6061 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
6121 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
6181 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
6241 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
6301 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
6361 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
6421 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
6481 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
6541 tttgcggccg gccgcaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
6601 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
6661 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
6721 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
6781 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
6841 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
6901 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
6961 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
7021 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
7081 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
7141 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
7201 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
7261 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
7321 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
7381 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
7441 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
7501 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
7561 cgcacatttc cccgaaaagt gccac
//