Table S3. Plasmids used in this study

Plasmid / Description / Reference or source
pET22b(+) / PT7, ApR, oripBBR322, lacI, C-term 6xHis, E. coli expression vector. / NovagenĀ®
pMM47.A / OriColE1, oripCC7, Apr ,Cfr, E. coli - C. canimorsus shuttle expression vector. / Mally and Cornelis (2008)
pPM5 / OriColE1, oripCC7, Apr ,Cfr, E. coli - C. canimorsus shuttle expression vector. / Manfredi, Lauber et al. (2014)
pFR11 / Full length E. coliglmS amplified with primers 7412 and 7413 and cloned into pPM5 using NcoI and XhoIrestriction sites. / This study
pFR20 / pMM47.A where the ermF promoter has been replaced by the mucpromoter: 340bp upstream of the mucA (Ccan_17470) ORF start codon were amplified with primers 6806 and 6807 and cloned into pMM47.A using SalI and NcoI restriction sites. / This study
pFR12 / Full length E. coliglmU amplified with primers 7406 and 7407 and cloned into pPM5 using NcoI and XhoIrestriction sites. / This study
pFR13 / Full lengthE. coliglmUandglmS co-amplified with primers 7406 and 7413 cloned into pPM5 using NcoI and XhoIrestriction sites. / This study
pFR14 / Full length E. coliglmM amplified with primers 7415 and 7416 and cloned downstream to the glmS gene into pFR11 using XhoIand SpeIrestriction sites. / This study
pFR15 / Full length E. coliglmM amplified with primers 7415 and 7416 and cloned downstream to the glmU and glmS genes into pFR13 using XhoI and SpeIrestriction sites. / This study
pFR16 / Full length E. coliglmM amplified with primers 7415 and 7416 and cloned downstream to the glmU gene into pFR12 using XhoI and SpeIrestriction sites. / This study
pFR17 / E.coliglmU del78 amplified with primers 7422 and 7407 and cloned into pFR16 using NcoI and XhoI restriction sites. / This study
pFR18 / E.coliglmU Tr331 amplified with primers 7406 and 7421 and cloned into pFR16 using NcoI and XhoI restriction sites. / This study
pFR19 / Full length Ccan_15070 with a C-terminal Strep tag amplified with primers 7442 and 7450 and cloned into pET22B(+) using NcoI and XhoIrestriction sites. / This study
pFR21 / Full lengthmucG (Ccan_17430) amplified with primers 6923 and 6925 and cloned into pFR20 using NcoI and XbaIrestriction sites. / This study
pFR22 / mucG (Ccan_17430) devoid of the first 21 aminoacids with a C-terminal Strep tag amplified with primers 7020 and 7021 and cloned into pET22b(+) using NcoI and XhoIrestriction sites. / This study
pFR23 / mucG (Ccan_17430) devoid of the first 355 aminoacids with a C-terminal Strep tag amplified with primers 7019 and 7021 and cloned into pET22b(+) using NcoI and XhoIrestriction sites. / This study
pFR24 / Full lengthmucC with a C-terminal Strep tag amplified with primers 6842 and 6786 and cloned into pFR20 using NcoIand XhoI restriction sites in frame with the 6xHis on the vector. / This study
pFR25 / Full lengthmucC amplified with primers 6842 and 6805 and cloned into pFR20 using NcoIand XhoI restriction sites. / This study
pFR26 / Full lengthmucD, mucE and mucG amplified with primers 6846 and 6845 and cloned into pFR24 using SpeIrestriction site. / This study
pFR27 / Full lengthmucD, mucE and mucG amplified with primers 6846 and 6845 and cloned into pFR25 using SpeIrestriction site. / This study
pFL38 / TetQ gene amplified from pMM104 with primers 7156 and 7157 and cloned into pPM5 using NcoIand XbaI restriction sites. / This study
pMM104 / ColE1 ori (pCC7 ori); Apr (Tcr); E. coli-C. canimorsus shuttle plasmid, RP4 oriT. PstI fragment of pMM47.A containing repA inserted into PstI site of pLYL001 / (Mally and Cornelis 2008)
pMM25 / oriColE1 , Kmr , Cfr . Suicide vector for C. canimorsus. / (Mally and Cornelis 2008)
pMM106 / oriColE1 , Kmr , Cfr , EryR, Mutator plasmid for the replacement of siaC / (Mally and Cornelis 2008)