Supplement B: Multiple Sequence Alignment of Tyrosinase Protein Sequences Obtained From

Supplement B: Multiple sequence alignment of tyrosinase protein sequences obtained from fungal source showing

maximum homology

gi|1519347|gb|AAB07484.1| ------MSTTGNIAITGIP--TTAGPDGSFPLRRELR 29

sp|P00440|TYRO_NEUCR ------MSTDIKFAITGVP--TPPSSNGAVPLRRELR 29

tr|A7FA30|A7FA30_SCLS1 ------MAAVPALNYPITGPPKASPGPADGSVPLRREIR 33

gi|154147835|emb|CAO82078.1| ------MTMKTYPITGVA------SQAPRPRRNIN 23

tr|B7VEU9|B7VEU9_TUBBO ------MTMKTYPITGVA------SQAPRPRRNIN 23

tr|B0DMA1|B0DMA1_LACBS ------MSRFVISGPG------GGAPN-RLEIR 20

sp|A7BHQ9|TYRO_PHONA ------MSRVVITGVS------GTIAN-RLEIN 20

gi|255687955|gb|ACU29457.1| ------MSDKKSLMPLVGIP------GEIKN-RLNIL 24

gi|27524354|emb|CAC82195.1| ------MSSNKPYVIKGIPVD----AGQIIPVRRDID 27

gi|238497704|ref|XP_002380087. ------MPYLITGIPKD----PKHPLPIRKDID 23

gi|255956845|ref|XP_002569175. ------MTSTEWKY------YPINGISVNSEEPSK-LGPEVQVPMRQNID 37

tr|C1H6P8|C1H6P8_PARBA ------MTAKTYKLPEYLSKRPYEYYAITGIKAGT-IPEQKKAPIRQEID 43

gi|225560938|gb|EEH09219.1| ------MAYKYYPIQGVQEG--LGPGSQVPIRRDFN 28

tr|C5FTV9|C5FTV9_NANOT MAGSLWRGLTVLYFLLCTTLPTFATVLVKGVQEG-VNQSTGQRPARQNLA 49

tr|C9SWR4|C9SWR4_VERA1 --MLLRFSLTVWVLAALASLVASQAYPITGVQVA----AGSAVPYRKNIN 44

: . * ::

gi|1519347|gb|AAB07484.1| DLQRN------YPDHFNLLVLALKDFQALN--ESVQTSYYQIAGIHGLPY 71

sp|P00440|TYRO_NEUCR DLQQN------YPEQFNLYLLGLRDFQGLD--EAKLDSYYQVAGIHGMPF 71

tr|A7FA30|A7FA30_SCLS1 DLQRN------FPDQWTLYILGLQAFQQLD--EKSDLSWYGIAGIHGRPY 75

gi|154147835|emb|CAO82078.1| DFAQ------DPLQWNLFLQALINLQSQGEDTHSPLGYYQVAGVHGTPY 66

tr|B7VEU9|B7VEU9_TUBBO DFAQ------DPLQWNLFLQALINLQSQGEDAHSPLGYYQVAGVHGTPY 66

tr|B0DMA1|B0DMA1_LACBS TFVQ------HDKYFSLYIQALQLMYTKESQAALQS-FFQVGGIHGLPY 62

sp|A7BHQ9|TYRO_PHONA DFVK------NDKFFSLYIQALQVMSSVPPQENVRS-FFQIGGIHGLPY 62

gi|255687955|gb|ACU29457.1| DFVK------NDKFFTLYVRALQVLQARD-QSDYSS-FFQLGGIHGLPY 65

gi|27524354|emb|CAC82195.1| EWYEDTSRQ--SRIQLSIFIWALREFQSID--YKDRLSYFQIAGIHHFPL 73

gi|238497704|ref|XP_002380087. DWYLEQTSAGSNRIQLTLFVEALTVIQNRP--LNDQLSYFRLAGIHGAPW 71

gi|255956845|ref|XP_002569175. TWSNN--PANEK--QVKLFVMALSRFQKID--PKERDSYFQIAGIHGQPN 81

tr|C1H6P8|C1H6P8_PARBA EWSNN--KANAD--QVDLFVMAWRNLMNTS--PRERGSFFQVAGIHGQPY 87

gi|225560938|gb|EEH09219.1| EWSES--QERRDQIQVVLFILALREFQATP--PDSRDSYFQIAGIHGMPY 74

tr|C5FTV9|C5FTV9_NANOT SWEFS------GPAFDLYVQALRDFQNTD--QKDIRSHYSIASVHGLPY 90

tr|C9SWR4|C9SWR4_VERA1 ILYKN------GGAPWDLYLRSLQDLYAQD--ANNRLSYFQIAGIHGRPY 86

: : . : : :..:* *

gi|1519347|gb|AAB07484.1| KPWNNVGSNSDWQS--TSGFGGYCTHSSILFLTWHRPYLALFEQALYNSI 119

sp|P00440|TYRO_NEUCR KPWAGVPSDTDWSQPGSSGFGGYCTHSSILFITWHRPYLALYEQALYASV 121

tr|A7FA30|A7FA30_SCLS1 RPWGGSQGDN------SPGWQGYCTHSSILFAPWHRPYLALFEQYLYQII 119

gi|154147835|emb|CAO82078.1| IPWMEKAD------ADDRAGDYCTHGTALFITWHRPYLLLFEQRIVEEA 109

tr|B7VEU9|B7VEU9_TUBBO IPWMEKAD------ADDRAGDYCTHGTALFLTWHRPYLLLFEQRIVEEA 109

tr|B0DMA1|B0DMA1_LACBS IPWDGVTG------IPNPTWGGYCTHGSVLFPTWHRPYVSAFEQILWTRA 106

sp|A7BHQ9|TYRO_PHONA TPWDGITGDQPF--DPNTQWGGYCTHGSVLFPTWHRPYVLLYEQILHKHV 110

gi|255687955|gb|ACU29457.1| TEWAKAQP------QLHLYKANYCTHGTVLFPTWHRAYESTWEQTLWEAA 109

gi|27524354|emb|CAC82195.1| ITWD----EEEPP---VPNKPGYCVHNNVTFPTWHRPYMLLFEQRLFEIM 116

gi|238497704|ref|XP_002380087. TEWDGVPGGQKDS---KGNPTGFCVHNNYTFPTWHRVYVTLYEQVIYEAM 118

gi|255956845|ref|XP_002569175. VPWDEP-IDAGDA---EGR--GYCTHNNILFPIWHRAYLALYEQRISEIM 125

tr|C1H6P8|C1H6P8_PARBA IPWDEPDTQMDEA---KDK--GYCTHNNILFPLWHRPYLVLLE------125

gi|225560938|gb|EEH09219.1| KSWDEHGLTVQET---HRK--GYCVHANSLFPIWHRPYLSLYEQRIYEIM 119

tr|C5FTV9|C5FTV9_NANOT GPWDGGMQ------SSRQQGYCLHDSTLFPLWHRPYLALHEVRRQQSN 132

tr|C9SWR4|C9SWR4_VERA1 IEWNNAGRRN------TNGWQGYCPHGEALFLPWHRPYVLAFEQRLVETA 130

* .:** * **** *

gi|1519347|gb|AAB07484.1| LASLR------TNVSLLLLS--YTNFDAFSFNRWDPNMT 264

sp|P00440|TYRO_NEUCR LASLR------NNVSLLLLS--YKDFDAFSYNRWDPNTN 267

tr|A7FA30|A7FA30_SCLS1 FDSYQ------SNIYLIMRDPNYKQFDAFSNHQWAANNA 270

gi|154147835|emb|CAO82078.1| GSSFR------DPIYKLLTL--VGSYGAFGNTGWQTGRP 264

tr|B7VEU9|B7VEU9_TUBBO GSSFR------DPIYKLLTL--VGSYGAFGNTGWQTGRP 264

tr|B0DMA1|B0DMA1_LACBS QSNIR------SNTYNLLTR--VTTWPAFSNHTVGDGGS 244

sp|A7BHQ9|TYRO_PHONA QENIT------SNTYSMLTR--VHTWKAFSNHTVGDGGS 249

gi|255687955|gb|ACU29457.1| APGFR------EWTFNMLTK--NYTWELFSNHGAVVG-A 250

gi|27524354|emb|CAC82195.1| LALNEHPWYRPNKAGFPPLQTLTYEVHRLLSFKFSSWGAFASTKWCN--E 297

gi|238497704|ref|XP_002380087. LALNEHPWYQ-NLDGWDSVPTLQDMTFRLLTTGGLNWGEFSSTRYDDKKE 307

gi|255956845|ref|XP_002569175. EAMKKPQWVADSPYGQP-----AEMVYRLLTVP-MEYSTFATTAQLSDNQ 296

tr|C1H6P8|C1H6P8_PARBA LALKSPQWMGEGKYGAA-----SEMVYRLLTHP-LDFISFATTFRSKGQD 204

gi|225560938|gb|EEH09219.1| KFIFDRKSITDFDYGKT-----TEMVYRLLTYQ-LDFVSFATTARDATMD 286

tr|C5FTV9|C5FTV9_NANOT GASLR------SSIYQLLSG--ESDYSTFSTQALPDRDG 277

tr|C9SWR4|C9SWR4_VERA1 RRSYR------SWTYDAMTR--SASFEEFASTGSSG--- 262

: : *.

gi|1519347|gb|AAB07484.1| PGEF------GSLEDVHNEIHDRTGGG------GHMS 289

sp|P00440|TYRO_NEUCR PGDF------GSLEDVHNEIHDRTGGN------GHMS 292

tr|A7FA30|A7FA30_SCLS1 PGTY------GSIEDVHNSVHSETGGN------GHMG 295

gi|154147835|emb|CAO82078.1| GPNN------ISLEHYHNIIHTFTGTNYIEE------NSKEGHMS 297

tr|B7VEU9|B7VEU9_TUBBO GPNN------ISLEHYHNIIHTFTGTNYIEE------NSKEGHMS 297

tr|B0DMA1|B0DMA1_LACBS TSN------SLEAIHDGIHVYVGGN------GQMG 267

sp|A7BHQ9|TYRO_PHONA TSN------SLEAIHDGIHVDVGGG------GHMG 272

gi|255687955|gb|ACU29457.1| HAN------SLEMVHNTVHFLIGRDPTLD------PLVPGHMG 281

gi|27524354|emb|CAC82195.1| ENKPPASQQT-RDILSLEYIHNNVHNWVGGTDYLG-DPSKPDLQGAGHMS 345

gi|238497704|ref|XP_002380087. EAQPKNNEQTPKNWMNLEAIHNNVHNWVGGFMFSRPGRHDLKLWGAGHMS 357

gi|255956845|ref|XP_002569175. D------VTNDINIEYLHNNIHGWVG------GDLNGHMS 324

tr|C1H6P8|C1H6P8_PARBA D------ISKDVNLEYIHNTVHGWVG------GNYTGHMS 232

gi|225560938|gb|EEH09219.1| SSSA----SKVINDMNIEFIHNNIHYWVG------GDG-GHMS 318

tr|C5FTV9|C5FTV9_NANOT YNN------VETVHGYVHSLVGGQ------GHMT 299

tr|C9SWR4|C9SWR4_VERA1 ------ISLEQIHNAVHWDGSCG------FQFL 283

:* *. :* . ::

gi|1519347|gb|AAB07484.1| SLDVSSFDPLFWFHHTNVDRLWAIWQDLNPDNFLTPRPAP---YSTFNST 336

sp|P00440|TYRO_NEUCR SLEVSAFDPLFWLHHVNVDRLWSIWQDLNPNSFMTPRPAP---YSTFVAQ 339

tr|A7FA30|A7FA30_SCLS1 DPDYAAFDPLFWLHHTNVDRQFAIWQALNPNSYAINKPSS---DGTFSIE 342

gi|154147835|emb|CAO82078.1| EVGVSAFDPIFWLHHCNVDRLYAIWQAIHYEAPFEDQATD---YTRMPLT 344

tr|B7VEU9|B7VEU9_TUBBO EVGVSAFDPIFWLHHCNVDRLYAIWQAIHYEAPFEDQATD---YTRMPLT 344

tr|B0DMA1|B0DMA1_LACBS DPAVAGFDPIFFLHHCNVDRLLSLWSALHHGVWVTPGPSQ---RGTLTIP 314

sp|A7BHQ9|TYRO_PHONA DPAVAAFDPIFFLHHCNVDRLLSLWAAINPGVWVSPGDSE---DGTFILP 319

gi|255687955|gb|ACU29457.1| SVPHAAFDPIFWMHHCNVDRLLALWQTMNYDVYVSEGMNR---EATMGLI 328

gi|27524354|emb|CAC82195.1| SVPVAAFDPIFWLYHNNVDRLTAIWQVLNQDHWFDEPHP------384

gi|238497704|ref|XP_002380087. SVPVAAFDPIFWLHHCNIDRLTAIWQTVNSGSWFNDDK------395

gi|255956845|ref|XP_002569175. QIPVASFDPMFWLHHCNIDRIFALWQALNPDKWFEKAKVNAFFQEIIGLP 374

tr|C1H6P8|C1H6P8_PARBA EIPVATFDPLFWLHHCNIDRMFAIWQALNPDKWFDTADKNTFFQEALGLA 282

gi|225560938|gb|EEH09219.1| QIPVATFDPIFWFHHCFLDRLFAIWQTLNPEKWFTADKTRPFDQKIIGMG 368

tr|C5FTV9|C5FTV9_NANOT YVPWSAYDPIFWLHHANVDRLIALWQAIYPDSYVQPIPNR---GGSYITQ 346

tr|C9SWR4|C9SWR4_VERA1 DADYSAFDPLFMLHHANVDPAAPASAPPG------312

: :**:* ::* :* .

gi|1519347|gb|AAB07484.1| EGESQTKDTPLTPFWDKSA----TKFWTSEEIKDTTTTFG------YAY 375

sp|P00440|TYRO_NEUCR EGESQSKSTPLEPFWDKSA----ANFWTSEQVKDS-ITFG------YAY 377

tr|A7FA30|A7FA30_SCLS1 SGAQETSTTALTPFNNATG----KTYWTSDGVRST-ATFN------YAY 380

gi|154147835|emb|CAO82078.1| -KAIDDAETTLRPFYKDECY---DVPWTSSMVQKSSAATGPTVFDYNYHY 390

tr|B7VEU9|B7VEU9_TUBBO -KAIDDAETTLRPFYKDECY---DVPWTSSMVQKSSAATGPTVFDYNYHY 390

tr|B0DMA1|B0DMA1_LACBS ANSTVDTNTNLTPFYHTQT-----AFWASAGVPDTTKLG------YTY 351

sp|A7BHQ9|TYRO_PHONA PEAPVDVSTPLTPFSNTET-----TFWASGGITDTTKLG------YTY 356

gi|255687955|gb|ACU29457.1| PGQVLTEDSPLEPFYTKNQ-----DPWQSDDLEDWETLG------FSY 365

gi|27524354|emb|CAC82195.1| --SDAKPDDPLKPFHVSKDK-----YFTSDDARFWRKYG------YDY 419

gi|238497704|ref|XP_002380087. --SKVSKDDDLRPFHRFCEKTRKVVFFRSDDVKDWRSLN------YDY 435

gi|255956845|ref|XP_002569175. DGTEITPNTGLRPFHKDAAG----TLMKPKDVRWTYKLG------YTY 412

tr|C1H6P8|C1H6P8_PARBA ET--ITPQTKLRPFHTDKVG----TCWTPEGARDTLNFG------YTY 318

gi|225560938|gb|EEH09219.1| NI--VTSKAPLRPFHMDEQG----TVWTPDGVRDWFKLG------YTY 404

tr|C5FTV9|C5FTV9_NANOT PGTIEDGNSPLYPFHANEN-----TYYTANTARYTRTFG------YTY 383

tr|C9SWR4|C9SWR4_VERA1 -GTSIGPNSPLQPFFAAPGR-----FHTPNSVRSIRGFG------YTY 348

*** . : *

gi|1519347|gb|AAB07484.1| PETQE--WKYRT------GSEYQTSIRQAVTTLYG--TNVFANFAA--- 411

sp|P00440|TYRO_NEUCR PETQK--WKYSS------VKEYQAAIRKSVTALYG--SNVFANFVENVA 416

tr|A7FA30|A7FA30_SCLS1 PETQQ--WKFPT------VQQYQTSVLAAVQKLYGATSNQFMQLMG--- 418

gi|154147835|emb|CAO82078.1| PELPVDLSGPGK------QKEMASHVLRRVHQLYGPP------421

tr|B7VEU9|B7VEU9_TUBBO PELPVDLSGPGK------QKEMASHVLRRVHQLYGPP------421

tr|B0DMA1|B0DMA1_LACBS PEFNG--LDTSN------PAAVQKAIAAIVNKLYGSAVFG------383

sp|A7BHQ9|TYRO_PHONA PEFNG--LDLGN------AQAVKAAIGNIVNRLYGASVFSGFAAAT--- 394

gi|255687955|gb|ACU29457.1| PDFDP--VKGKS------KEEKSVYINDWVHKHYG------392

gi|27524354|emb|CAC82195.1| DIVKK--PGTN------EDRAPEEVKMKINQLYG------445

gi|238497704|ref|XP_002380087. AITKD--ASR------IRKEISDLYG------453

gi|255956845|ref|XP_002569175. PELDT--WNFK---PEGYTSENFISNLRKTINDLYG------443

tr|C1H6P8|C1H6P8_PARBA PELQT--WDAKYQAAGAYNNELHLTDLKKAINEKYG------352

gi|225560938|gb|EEH09219.1| PELQR--WEYG------GDYKDELFRDMNDMYG------429

tr|C5FTV9|C5FTV9_NANOT PEVED--WGVSK------ADLEKNVRRHVNELYNNPTSQPKKRTTVHR 423

tr|C9SWR4|C9SWR4_VERA1 EGLEY--WKKND------GQLQRDATALINKYYATG------376

: *