DEFENSE RELATED PHYTOHORMONES REGULATION IN MYCORRHIZAS DEPENDS ON THE PARTNER GENOTYPES
JOURNAL OF CHEMICAL ECOLOGY
FERNÁNDEZ I., MERLOS M., LÓPEZ-RÁEZ J.A., MARTÍNEZ-MEDINA A., FERROL N., AZCÓN C., BONFANTE P., FLORS V., POZO M.J.*
* Department of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidín (CSIC), Granada, Spain. E-mail:
SUPPLEMENTARY
Figure S1. ROOT AND SHOOT FRESH WEIGHTS IN TOMATO PLANTS, SOYBEAN AND MAIZE COLONIZED BY F. mosseae (Fm) OR R. irregularis (Ri).
Nm indicates control non-mycorrhizal plants. Numbers represent the average of 5 independent replicates ±SE. Data not sharing a letter in common differ significantly (P ≤0.05) according to DMS’s test.
Table S1. PRIMERS USED FOR GENE EXPRESSION ANALYSES IN THIS STUDY
ID / Tomato Gene / Primers (5’-3’)X14449
/ Elongation factor 1α
(Sl-EF1)4 / GATTGGTGGTATTGGAACTGTC
AGCTTCGTGGTGCATCTC
X51904
/ Desiccation protective protein
(Sl-Le4)2 / ACTCAAGGCATGGGTACTGG
CCTTCTTTCTCCTCCCACCT
M69247 / Pathogenesis related protein
(Sl-PR-1a)2 / GTGGGATCGGATTGATATCCT
CCTAAGCCACGATACCATGAA
U09026 / Lipoxygenase A
(Sl-LOXA)2 / GGTTACCTCCCAAATCGTCC
TGTTTGTAACTGCGCTGTG
X58253 / Ubiquitin
(Sl-Ubiquitin)1 / ACCAAGCCAAAGAAGATCAAGC
GTGAGCCCACACTTACCACAGT
AY885651
/ Phosphate transporter
(Sl-PT4)5 / GAAGGGGAGCCATTTAATGTGG ATCGCGGCTTGTTTAGCATTTC
HE817883.1 / Rhizofagous irregularis
(Fm 18S rARN)6 / TGTTAATAAAAATCGGTGCGTTGC AAAACGCAAATGATCAACCGGAC
AJ271093
/ Allene oxide synthase
(Sl-AOS1)2 / CACCTGTTAAACAAGCGAAAC TCCAGCTCGTTCACCTTCTT
AF454634
/ Allene oxide synthase
(Sl-AOS3)2 / GCGGAGGAGTTCAATCCAG CGCATGAAAAACTCCACAACC
AF083253
/ Multicystatin
(Sl-MC)3 / GAGAATTTCAAGGAAGTTCAA GGCTTTATTTCACACAGAGATA
AF506005.1 / Cell-wall Invertase
(Sl-LIN6)1 / AGCACATTTATTCGCCTTCAACAA TTTGTGACGTGGCATAATAAGAT
1This work; 2Lopez-Raez et al., 2010; 3Uppalapati et al., 2005; 4Rotenberg et al., 2006; 5Balestrini et al., 2007; 6Porcel et al., 2005
ID / Soybean Gene / Primers (5’-3’)Glyma02g44460 / Elongation factor 1β
(Gm-EF1)2 / AAGGGAGGCTGCTAAAAAGC CAACTGTCAAGCGTTCCTCA
NM_001248671 / Desiccation protective protein
(Gm-Lea)4 / GTCGACGAGATACGGCTAACCC CCAGGGCAAGCTTTCTCCTTT
NM_001251239 / Pathogenesis related protein
(Gm-PR-1a)3 / ATGTGTGTGTTGGGGTTGGT ACTTTGGCACATCCAAGACG
AI443430 / Lipoxygenase
(Gm-LOX)1 / CCGAGAGCATCCAAATACAA GCTCTATTATCGTTTGGACA
1Moy et al., 2004; 2Yamaguchi et al., 2011; 3 de Roman et al., 2011; 4 Porcel et al., 2005
ID / Maize Gene / Primers (5’-3’)Nm_001112117 / Elongation factor 1α
(Zm-EF1)1 / TGTTGCTGTGAAGGATCTGAA ATGATGATGACCTGGGAGG
GU237073.1 / Desiccation protective protein
(Zm-ABP9)1 / TCCAGTCCGCTTGCTTAGTT GTTTGTACAACCCAGGCTCA
NM_001159109.1 / Pathogenesis related protein
(Zm-PR-1a)1 / AGAACCTCTTCTGGGGCAGT GGGTCGTAGTTGAGGTGAT
NM_001112510.1 / Lipoxygenase 10
(Zm-LOX10)1 / ATCCTCAGCATGCATTAGTCC AGTCTCAAACGTGCCTCTTGTT
1This work
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