SUPPLEMENTARY DATA
Stress Challenge
Using a Chi square (χ2) test, we found no associations between diagnosis and COMT (χ2 = 0.658, p = 0.720, df = 2), between diagnosis and MAOA (χ2 = 0.812, p = 0.368, df = 1), diagnosis and 5-HTT (χ2 = 1.590, p = 0.45, df = 4) and between diagnosis and Sex (χ2 = 3.182, p = 0.074, df = 1). Likewise, no significant associations between MDRisk and COMT (χ2= 2.609, p = 0.271, df = 2), MDRisk and MAOA (χ2= 0.065, p = 0.799, df = 1), MDRisk and 5-HTT (χ2 = 0.245, p = 0.885, df = 2), and between MDRisk and sex (χ2 = 2.610, p = 0.106, df = 1) were observed. We found no association between COMT and Sex using (χ2 = 2.651, p = 0.266, df = 2), however, a significant association between MAOA and Sex was found (χ2 = 5.833, p = 0.016, df = 1) suggesting higher frequency of male representation within the group carrying the low activity MAOA while high activity allelic carriers were predominantly women (table 1h), this was not surprising given that MAOA is an X linked gene. No significant association between 5-HTT and sex was found (χ2 = 3.873, p = 0.144, df = 2). Finally, we found no significant COMT by MAOA associations (χ2 = 2.080, p = 0.353, df = 2; table 2i)n COMT and 5-HTT (χ2 = 4.909, p = 0.297, df = 4) and between MAOA and 5-HTT (χ2 = 0.078, p = 0.962, df = 2). For a summary of the demographics see TableS1.
Table1. COMT By Diagnosis
MD no MD (i.e., HRPs and healthy controls) Total
COMT
met/met 8 13 21
val/met 10 26 36
val/val 4 9 13
Total 22 48 70
. MAOA By diagnosis
COMT
Low 3 11 14
High 19 37 56
Total 22 48 70
Sex By diagnosis
COMT
male 6 24 30
female 16 24 40
Total 22 48 70
COMT By MDRisk MDRisk no MDRisk (i.e., healthy controls)
COMT
met/met 16 5 21
val/met 21 15 36
val/val 10 3 13
Total 47 23 70
MAOA By MDRisk
COMT
Low 9 5 14
High 38 18 56
Total 47 23 70
Sex By MDRisk
Sex
male 17 13 30
female 30 10 40
Total 47 23 70
COMT By Sex male female
COMT
met/met 7 14 21
val/met 15 21 36
val/val 8 5 13
Total 30 40 70
MAOA By Sex
MAOA
Low 10 4 14
High 20 36 56
Total 30 40 70
COMT By MAOA Low High
COMT
met/met 2 19 21
val/met 9 27 36
val/val 3 10 13
Total 14 56 70
5-HTT By diagnosis MD no MD
SS 13 3 16
LS 23 13 36
LL 6 12 18
48 24
5-HTT By MDRisk MDRisk no MDRisk
SS 10 6 16
LS 11 25 36
LL 12 6 18
33 37
5-HTT By sex male female
SS 10 6 16
LS 12 24 36
LL 8 10 18
30 40
5-HTT By MAO Low High
SS 3 13 16
LS 7 29 36
LL 4 14 18
14 56
5-HTT By COMT met/met val/met val/val
SS 5 10 1 16
LS 11 15 10 36
LL 5 11 2 18 21 36 13
Table 1. (a) Mean of group demographics by genotypes.
DEX/CRH Challenge
We found no associations between diagnosis and COMT (χ2, p = 0.693, df = 2; see table 3a), between diagnosis and MAOA (χ2, p = 0.158, df = 1; see table 3b) and between diagnosis and Sex (χ2, p = 0.077 (df = 1; table 3c). Likewise, no significant associations between the existence of MDRisk and COMT (χ2, p = 0.415, df = 2; table 3d), MDRisk and MAOA (χ2, p = 0.158, df = 1; table 3e) and no association was found between Sex and MDRisk (χ2, p = 0.081, df = 1; see table 3f). We found no association between COMT and Sex using (χ2, p = 0.978, df = 2; table 3g), however, a near significant association between MAOA and Sex was found (χ2, p < 0.067, df = 1; table 3h), this was not surprising given that MAOA is an X linked gene, with only females in the heterozygous group, while males can only be homozygous. Finally, we found no significant COMT by MAOA associations (χ2, p = 0.186, df = 2; table 1i). For a summary of the demographics see TableS1.
Table 2a. COMT By Diagnosis
MD no MD (i.e., HRPs and healthy controls) Total
COMT
met/met 7 11 18
val/met 9 24 33
val/val 4 9 13
Total 20 44 64
2b. MAOA By diagnosis
MAOA
Low 3 14 17
High 17 30 47
Total 20 44 64
2c. Sex By diagnosis
Sex
male 7 26 33
female 13 18 31
Total 20 44 64
2d. COMT By MDRisk MDRisk no MDRisk (i.e., healthy controls)
COMT
met/met 15 3 18
val/met 22 11 33
val/val 10 3 13
Total 47 17 64
2e. MAOA By MDRisk
MAOA
Low 12 5 17
High 35 12 47
Total 47 17 64
2f. Sex By MDRisk
Sex
male 21 12 33
female 26 5 31
Total 47 17 64
1g. COMT By Sex
male female
COMT
met/met 9 9 18
val/met 17 16 33
val/val 7 6 13
Total 33 31 64
1h. MAOA By Sex
COMT
Low activity 12 5 17
High 21 26 47
Total 33 31 64
1i. COMT by MAOA
Low High
COMT
met/met 3 15 18
val/met 12 21 33
val/val 2 11 13
Total 17 47 64
Table 2. Mean of group demographics by genotypes.