Fungal Genetics Stock Center

Plasmid Number

(for FGSC use)

Fungal Genetics Stock Center

Dept. of Microbiology

Univ. of Kansas Medical Center

Kansas City, KS 66160-7420

Reprints or other data relating to this deposit will aid the Stock Center and recipients of the plasmid. Reprints included.

Plasmid name: pAO81

Gene: S-TAG + A. fumigatus pyrG

Host Strain: DNA sent

Form in which supplied: 10ug DNA in Ethanol

Your Name: Stephen A. Osmani

Literature Reference:

Yang L, Ukil L, Osmani A, Nahm F, Davies J, De Souza CP, Dou X, Perez-Balaguer A, Osmani SA. (2004) Rapid production of gene replacement constructs and generation of a green fluorescent protein-tagged centromeric marker in Aspergillus nidulans. Eukaryotic Cell. 3:1359-1362

Other Information (eg construction details, accession numbers, other references):

Cloned into In vitrogen vector pCR-BluntII-TOPO

Map: (Please draw or attach a map showing restriction sites, polylinkers, promoters, etc): Attached

Sequence of the GA4-S-Tag + A. fumigatus pyrG is given below plasmid map

GGAGCTGGTGCAGGCGCTGGAGCCAAAGAAACCGCTGCTGCTAAATTCGAACGCCAGCACATGGACAGCTAATGCTCTTCACCCTCTTCGCGGGTCTGAAATACCCTCACCTGGCAACAGCAATTGGCGCTTCATGGCTGTTTTTCCGATCTCTCTACTTGTACGGCTATGTGTACTCGGGTAAGCCACAAGGCAAGGGCAGATTGCTGGGAGGTTTCTTCTGGTTTTCTCAAGGCGCTCTGTGGGCTCTGAGTGTGTTTGGTGTTGCCAAAGACATGATCTCTTACTGAGAGTTATTCTGTGTCTGACGAAATATGTTGTGTATATATATATATGTACGTTAAAAGTTCCGTGGAGTTACCAGTGATTGACCAATGTTTTATCTTCTACAGTTCTGCCTGTCTACCCCATTCTAGCTGTACCTGACTACAGAATAGTTTAATTGTGGTTGACCCCACAGTCGGAGGCGGAGGAATACAGCACCGATGTGGCCTGTCTCCATCCAGATTGGCACGCAATTTTTACACGCGGAAAAGATCGAGATAGAGTACGACTTTAAATTTAGTCCCCGGCGGCTTCTATTTTAGAATATTTGAGATTTGATTCTCAAGCAATTGATTTGGTTGGGTCACCCTCAATTGGATAATATACCTCATTGCTCGGCTACTTCAACTCATCAATCACCGTCATACCCCGCATATAACCCTCCATTCCCACGATGTCGTCCAAGTCGCAATTGACTTACGGTGCTCGAGCCAGCAAGCACCCCAATCCTCTGGCAAAGAGACTTTTTGAGATTGCCGAAGCAAAGAAGACAAACGTTACCGTCTCTGCTGATGTGACGACAACCCGAGAACTCCTGGACCTCGCTGACCGTACGGAAGCTGTTGGATCCAATACATATGCCGTCTAGCAATGGACTAATCAACTTTTGATGATACAGGTCTCGGTCCCTACATCGCCGTCATCAAGACACACATCGACATCCTCACCGATTTCAGCGTCGACACTATCAATGGCCTGAATGTGCTGGCTCAAAAGCACAACTTTTTGATCTTCGAGGACCGCAAATTCATCGACATCGGCAATACCGTCCAGAAGCAATACCACGGCGGTGCTCTGAGGATCTCCGAATGGGCCCACATTATCAACTGCAGCGTTCTCCCTGGCGAGGGCATCGTCGAGGCTCTGGCCCAGACCGCATCTGCGCAAGACTTCCCCTATGGTCCTGAGAGAGGACTGTTGGTCCTGGCAGAGATGACCTCCAAAGGATCGCTGGCTACGGGCGAGTATACCAAGGCATCGGTTGACTACGCTCGCAAATACAAGAACTTCGTTATGGGTTTCGTGTCGACGCGGGCCCTGACGGAAGTGCAGTCGGATGTGTCTTCAGCCTCGGAGGATGAAGATTTCGTGGTCTTCACGACGGGTGTGAACCTCTCTTCCAAAGGAGATAAGCTTGGACAGCAATACCAGACTCCTGCATCGGCTATTGGACGCGGTGCCGACTTTATCATCGCCGGTCGAGGCATCTACGCTGCTCCCGACCCGGTTGAAGCTGCACAGCGGTACCAGAAAGAAGGCTGGGAAGCTTATATGGCCAGAGTATGCGGCAAGTCATGATTTCCTCTTGGAGCAAAAGTGTAGTGCCAGTACGAGTGTTGTGGAGGAAGGCTGCATACATTGTGCCTGTCATTAAACGATGAGCTCGTCCGTATTGGCCCCTGTAATGCCATGTTTTCCGCCCCCAATCGTCAAGGTTTTCCCTTTGTTAGATTCCTACCAGTCATCTAGCAAGTGAGGTAAGCTTTGCCAGAAACGCCAAGGCTTTATCTATGTAGTCGATAAGCAAAGTGGACTGATAGCTTAATATGGAAGGTCCCTCAGGGACAAGTCGACCTGTGCAGAAGAGATAACAGCTTGGCATCACGCATCAGTGCCTCCTCTCAGACAG

2