Curriculum Vitae Date of birth: 09. February, 1972
Nationality: Croatian, Family status: married, daughter 9 y, son 5 y
Address: Kosa 14, 10000 Zagreb
Ivo Crnolatac Mobile:+385 91 542 1474
e-mail:
Profile: Experienced Researcher highly skilled in a range of different areas of Pharmaceutical Research and Drug Discovery.
Major competencies in: protein purification and structure elucidation, chromatography, in vitro cell culture techniques, organic synthesis.
Professional experience:
Sept 2008 – Senior Research Assistant Ruder Boskovic Institute
Division of Organic Chemistry and Biochemistry, Laboratory for the study of interactions of Biomacromolecules, Zagreb, Croatia
Research projects involving spectroscopic studies (UV/Vis, thermal melting, fluorescence spectroscopy and CD spectropolarimetry) to estimate the binding constants and binding densities of small molecules on biomacromolecules. In vitro testing of biologically active candidates.
Feb 2008 – March 2009 Researcher-Process Engineer at PLIVA Croatia Ltd, Biologics R&D
Biotechnology, Isolation and Purification of biomolecules, Zagreb, Croatia
Process development (DOE), technology transfer- isolation and purification of bioequivalent recombinant proteins. Responsible for collection of reports and performing studies necessary for the registration of the biogeneric drug substance, in charge of stability, container/closure: extractables/leachables and comparability studies.
Feb 2002 – Jan 2008 Researcher at the Lab of Pharmacertutical Biology and Phytopharmacology
Faculty of Pharmaceutical Sciences, Katholieke Universiteit Leuven, Belgium
Post-doctoral research, Nano Psy project: Testing and formulation development of various nano particles for their prospective use as treatment or diagnostic modalities for cancer.
PhD Project: Elucidation of tumouritropic principle of hypericin.
Hypericin is a naturally occurring photosensitiser which is successfully used in clinical diagnostics of early stage bladder cancer. Project involved chemical modification of the parent molecule and evaluation of the selective cellular uptake and photocytotoxicity.
May 1998 – May 2001 Scientist, anti-infectives research
Pliva Pharmaceuticals, Research & Development, Zagreb, Croatia
Scale up and development of downstream processes for bacterial fermentations and structure elucidation of various new compounds. Analytical and chemical support of multi disciplinary Biodiversity project.
May 1996 – May 1998 Scientist, anti-infectives research
Pliva Pharmaceuticals, Research & Development, Zagreb, Croatia
Downstream processing of bacterial fermentations (genetically modified Streptomyces), structure elucidation of new tetracycline compounds.
Professional Development
1996 - 1998 Courses of Business communication, Presentation and Negotiation skills at Lancon English Consultancy
Jul 1997 – Sept 1997 Specialisation at University of Kaiserslautern, Germany (Downstream Processing)
Jul 1998 – Dec 1998 Specialisation at the Faculty of Pharmaceutical Sciences, University of Strathclyde, Glasgow, UK (NMR-Operating, Spectroscopy Based Structure Elucidation)
Oct 2005 – Mar 2006 Advanced Course in Laboratory Animal Science: Certificate - Laboratory Animal Leader
May 2009 – Nov 2009 Marketing management course at IVORA IT & Management, Cambridge International Examinations Certificate
Education
Jun 2003 – Dec 2006 Faculty of Pharmaceutical Sciences, Katholieke Universiteit Leuven
PhD Pharmaceutical Sciences
Feb 2002 – Jun 2003 Faculty of Pharmaceutical Sciences, Katholieke Universiteit Leuven
Master of Pharmaceutical Sciences (Magna cum laudum)
1990 – 1995 University of Zagreb, Faculty of Chemical Engeneering and Technology
University degree in Chemical engineering and technology, field of organic processes
Languages Croatian (mother tongue); Fluent in English and Russian, good knowledge of French, basic understanding of Dutch and German
Hobbies reading, handball, football, basketball.
Bibliography
1. Purification of Nano-Porous Silicon for Biomedical Applications
Koynov S, Pereira RN, Crnolatac I, Kovalev D, Huygens A, Chirvony V, Stutzmann M, de Witte P
Advanced Engineering Materials 13(6): B225–B233 2011 June.
2. Differential accumulation of hypericin in spheroids composed of T-24 transitional cell carcinoma cells expressing different levels of e-cadherin.
Huygens A, Crnolatac I, Develter J, Van Cleynenbreugel B, Van der Kwast T, and de Witte PA
The Journal of urology 179(5):2014-9 2008 May
3. Influence of the glycosaminoglycan layer on the permeation of hypericin in rat bladders in vivo.
Huygens A, Crnolatac I, Maes J, Van Cleynenbreugel B, Van Poppel H, Roskams T, and de Witte PA
BJU international 100(5):1176-81 2007 Nov
4. In vitro accumulation and permeation of hypericin and lipophilic analogues in 2-D and 3-D cellular systems.
Crnolatac I, Huygens A, Agostinis P, Kamuhabwa AR, Maes J, van Aerschot A, and De Witte PA
International journal of oncology 30(2):319-24 2007 Feb
5. Synthesis, in vitro cellular uptake and photo-induced antiproliferative effects of lipophilic hypericin acid derivatives.
Crnolatac I, Huygens A, van Aerschot A, Busson R, Rozenski J, and de Witte PA
Bioorganic & medicinal chemistry 13(23):6347-53 2005 Dec 1
6. Novel hybrid polyketide compounds produced by genetic engineering of the oxytetracycline biosynthetic pathway
Hranueli, D., N. Perić, H. Petković, G. Biuković, Z. Toman, J. Pigac, B.Borovička, A. Bago, I. Crnolatac, T. Maršić, L. Zhou, S. Matošić, P. G. Waterman, J. Cullum, and I. S. Hunter.
Food Technol. Biotechnol. 37:117–125 1999.