Table 1 Characterization of 16 polymorphic microsatellite loci developed for Andrias davidianus.

Locus / GenBank accession no. / Primer sequence / Motif / Ta / Size of alleles / A / LY (n = 19) / TB (n = 17)
He / Ho / PIC / FIS / He / Ho / PIC / FIS
CIBad01 / KF791316 / F:TGAGAACTTCCGCCTGAA
R:CGCTACATAAGACCGAATATAG / (TATC)23 / 58 / 274-298 / 7 / 0.81 / 0.89 / 0.76 / 0.21 / 0.75 / 0.71 / 0.68 / 0.01*
CIBad02 / KF791317 / F:GTGCTGCTACAATTCAACA
R:GCTGTGTGCAACATTAGATA / (AGAT)18g(GATA)8 / 58 / 188-232 / 6 / 0.67 / 0.89 / 0.59 / 0.01* / 0.54 / 0.59 / 0.41 / 0.45
CIBad03 / KF791318 / F:CCTTCCTCTCATCTCTTCAAT
R:AACTAACTCTCGGTCTTACA / (CTAT)28 / 53 / 266-302 / 7 / 0.78 / 0.89 / 0.72 / 0.12 / 0.73 / 0.82 / 0.66 / 0.13
CIBad04 / KF791319 / F:GCATTGTAGCTGGATTGAC
R:GGGTTGTTGACAATGTTTCA / (ATCT)25 / 58 / 206-242 / 9 / 0.89 / 0.95 / 0.85 / 0.23 / 0.74 / 0.63 / 0.67 / 0.05*
CIBad05 / KF791320 / F:GGCACTACAACAAGACCAA
R:TTAGGAGCATAGACACTGAAG / (GATA)23 / 58 / 139-187 / 8 / 0.80 / 0.89 / 0.74 / 0.20 / 0.63 / 0.69 / 0.53 / 0.03*
CIBad06 / KF791321 / F:GGTGGAAGTTGCTGGAAT
R:GTACCTTCGGGTGTAATGG / (TCTA)20 / 58 / 222-258 / 8 / 0.86 / 0.80 / 0.81 / 0.33 / 0.70 / 0.76 / 0.63 / 0.34
CIBad07 / KF791322 / F:CTATTGTGCCCTGAAGTGT
R:CGCATGATGGATTACTATGG / (TATC)23 / 56 / 195-223 / 6 / 0.79 / 0.89 / 0.73 / 0.28 / 0.71 / 0.86 / 0.62 / 0.15
CIBad08 / KF791323 / F:CTGACCTGGCTACCTGAT
R:ATGTGTGCTCTATGCTCTT / (ATCT)17 / 58 / 250-278 / 8 / 0.78 / 0.74 / 0.72 / 0.39 / 0.76 / 0.88 / 0.70 / 0.34
CIBad09 / KF791324 / F:AGCATTACACAGGTCAAGA
R:CACTACCGTAATGCTGGTT / (TAGA)19 / 55 / 232-260 / 8 / 0.76 / 0.79 / 0.71 / 0.42 / 0.62 / 0.71 / 0.52 / 0.31
CIBad10 / KF791325 / F:CTTGCTCAGAGTTCCACAA
R:CGCATTATTCTCTATCACTTCC / (ATCT)17 / 55 / 241-265 / 6 / 0.76 / 0.74 / 0.70 / 0.24 / 0.71 / 0.88 / 0.63 / 0.49
CIBad11 / KF791326 / F:CTAACACCACACGCTCTAT
R:TGAACTCACTTCTGCTCTAA / (TAGA)5tgcatg(TATC)12 / 53 / 296-332 / 7 / 0.79 / 0.79 / 0.74 / 0.45 / 0.60 / 0.65 / 0.49 / 0.42
CIBad12 / KF791327 / F:CAGTGAGGTTGGCATCTAA
R:TGGTAAGTTGTATCAGGTGTC / (TCTA)14 / 58 / 300-332 / 7 / 0.87 / 0.89 / 0.83 / 0.41 / 0.44 / 0.35 / 0.40 / 0.06
CIBad13 / KF791328 / F:CAGCATTCATTAGCCTTATCAC
R:ACTAGCCGTTGTCACAGA / (AGAT)9agg(TAGA)6 / 57 / 257-281 / 4 / 0.68 / 0.59 / 0.59 / 0.14 / 0.65 / 0.63 / 0.58 / 0.43
CIBad14 / KF791329 / F:GGTGATAGCTGCATGGAAT
R:AAACGCTATGGGCATCTC / (AGAT)21 / 59 / 267-311 / 10 / 0.82 / 0.84 / 0.78 / 0.21 / 0.67 / 0.71 / 0.59 / 0.00*
CIBad15 / KF791330 / F:GAGGCAGATGAACTAGGAAT
R:AGAATGTGGTAGGAGTAAGTC / (TCTA)18 / 58 / 272-332 / 10 / 0.76 / 0.88 / 0.71 / 0.12 / 0.67 / 0.60 / 0.58 / 0.03*
CIBad16 / KF791331 / F:CTCCAGCATTAGCAAACG
R:AAGGGTGGTTATTATTAGCG / (TATC)20 / 58 / 324-384 / 8 / 0.82 / 0.89 / 0.77 / 0.14 / 0.74 / 0.69 / 0.67 / 0.05

The primer pair, annealing temperature in ℃ (Ta), repeat motif, number of alleles (A) and size of alleles (bp) screened in 36 individuals.

The expected (HE) and observed (HO) heterozygosities, polymorphism information content (PIC), and exact P-values by the Markov Chain method of Hardy-Weinberg test are reported in two populations (LY, TB).