SUPPLEMENTAL MATERIAL
Supplemental Table 1: Sequence of the primers used for validation of gene signatures. Tm: melting temperature
Gene / Primer Sequence / Tm (°C)ASB5 / Forward / ggagcagacatcaatgcaaa / 53.5
ASB5 / Reverse / agttggcaaagagagcttgg / 55.5
C1QA / Forward / ccgggtctcaaaggagagag / 56.8
C1QA / Reverse / agattcccctgggtctcct / 57.4
CAR3 / Forward / tcaaagagcccatgactgtg / 55.0
CAR3 / Reverse / cccttgacaggctgaggag / 57.5
CCL8 / Forward / taaggctccagtcacctgct / 58.2
CCL8 / Reverse / ataccctgcttggtctggaa / 55.9
CCL9 / Forward / accagtggtgggtgtaccag / 59.2
CCL9 / Reverse / ttgtaggtccgtggttgtga / 56.1
CD74 / Forward / atgacccaggaccatgtgat / 56.0
CD74 / Reverse / ctgcttcatccagctctcg / 55.5
CTLA2B / Forward / gggcaagaccagcttctaca / 57.1
CTLA2B / Reverse / tccacagcaatttgtcctga / 54.4
CUGBP2 / Forward / acccaggcctactcaggaat / 57.8
CUGBP2 / Reverse / gattgcagcttgtgcagaga / 55.8
FABP4 / Forward / gcgtggaattcgatgaaatca / 53.6
FABP4 / Reverse / cccgccatctagggttatga / 56.3
GAPDH / Forward / catggccttccgtgttccta / 63.3
GAPDH / Reverse / cctgcttcaccaccttcttga / 62.7
GLUL / Forward / tagcaacctttgaccccaag / 55.0
GLUL / Reverse / actggtgcctcttgctcagt / 58.9
GRS5 / Forward / gaagatcaagtcccccatca / 54.1
GRS5 / Reverse / tccaccaggttcttcatggt / 56.1
IFI27 / Forward / ttgccaatggaggtggagttgcag / 61.9
IFI27 / Reverse / atcagtgagggttctgaaggtgcc / 60.8
IGF1 / Forward / tggatgctcttcagttcgtg / 54.9
IGF1 / Reverse / gggaggctcctcctacattc / 56.8
IIGP1 / Forward / tttgccctccagctttactg / 55.3
IIGP1 / Reverse / tcactgattgcagagtttgtca / 54.5
LAPTM5 / Forward / gcagctccaagatgttcctc / 55.9
LAPTM5 / Reverse / ctggtggggatcacacttct / 56.6
LPL / Forward / atccatggatggacggtaacg / 56.9
LPL / Reverse / ctggatcccaatacttcgacca / 56.3
LYZS / Forward / ctgtgggatcaattgcagtg / 54.1
LYZS / Reverse / gggacagatctcggttttga / 54.4
MRC1 / Forward / cttcgggcctttggaataat / 52.9
MRC1 / Reverse / tagaagagcccttgggttga / 55.5
MS4A6D / Forward / tcagcagtgtgttggagctt / 56.9
MS4A6D / Reverse / tgagttctgagacaggaaaatca / 53.7
MSA2A6B / Forward / ctgtcctcactgccatgct / 57.5
MSA2A6B / Reverse / tgagctatgtggcaggaaca / 56.3
POSTN / Forward / tccacaggaggtggagaaac / 56.5
POSTN / Reverse / gcctctgggttttcactgag / 55.8
SEPP1 / Forward / cgcttactgtgaggagaggtg / 57.1
SEPP1 / Reverse / tggtgtttgtggtggctatg / 55.5
SERPING1 / Forward / aaagagcagccaagacatgc / 56.1
SERPING1 / Reverse / gtctcgtgtttcatggcaga / 55.1
TRPT1 / Forward / ttctgactccagggaatgct / 55.8
TRPT1 / Reverse / ttcctcctctggaactgagc / 56.3
Supplemental Table 2. 79 genes up-regulated in tumors resected at estrus stage compared to tumors resected at diestrus stage© Genes are listed in descending order of EC vs. DC fold difference. EC: EC-pooled; DC: DC-pooled; DNC: DNC-pooled; NS: not significantly differentially expressed
Gene Symbol / Sequence Code / Sequence Description / EC/DNC / EC/DC / DC/ DNCGLYCAM1 / 1424825_a_at / Endothelial ligand for L-selectin (GLYCAM1) / 100.00 / 100.00 / NS
IGH-1A / 1451632_a_at / Immunoglobulin heavy chain 1a (serum IgG2a), mRNA (cDNA clone MGC:6529 IMAGE:2651493) / 100.00 / 100.00 / NS
IGH-6 / 1427351_s_at / Immunoglobulin heavy chain 6 (heavy chain of IgM), mRNA (cDNA clone MGC:18788 IMAGE:4189350) / 100.00 / 100.00 / NS
IGJ / 1424305_at / Immunoglobulin joining chain (Igj), mRNA / 100.00 / 90.60 / NS
CCL21A / 1419426_s_at / Chemokine (C-C motif) ligand 21b, mRNA (cDNA clone MGC:47896 IMAGE:3372243) / 72.72 / 78.54 / NS
MS4A1 / 1423226_at / Membrane-spanning 4-domains, subfamily A, member 1, mRNA (cDNA clone MGC:36191 IMAGE:4503841) / 48.48 / 46.05 / NS
IGL-V1 / 1430523_s_at / Immunoglobulin lambda chain (IgL) / 93.59 / 44.91 / NS
CD79A / 1418830_at / CD79A antigen (immunoglobulin-associated alpha), mRNA (cDNA clone MGC:41188 IMAGE:1332396) / 36.97 / 44.77 / NS
CXCL13 / 1417851_at / Chemokine (C-X-C motif) ligand 13, mRNA (cDNA clone MGC:13851 IMAGE:4217539) / 23.61 / 26.20 / NS
CD3D / 1422828_at / CD3 antigen, delta polypeptide (Cd3d), mRNA / 22.09 / 21.40 / NS
ADIPOQ / 1422651_at / Adiponectin, C1Q and collagen domain containing, mRNA (cDNA clone MGC:41360 IMAGE:1329799) / 23.98 / 17.93 / NS
HP / 1448881_at / Haptoglobin (Hp), mRNA / 13.93 / 17.31 / NS
CCL19 / 1449277_at / Chemokine (C-C motif) ligand 19, mRNA (cDNA clone IMAGE:4920139) / 13.94 / 15.01 / NS
H2-OB / 1440837_at / Transcribed locus / 10.71 / 13.86 / NS
IGK-V19-14 / 1427860_at / IgG light chain gene, V region / 13.99 / 13.57 / NS
TCRB-V13 / 1425854_x_at / T-cell receptor beta, variable 13, mRNA (cDNA clone MGC:41373 IMAGE:1365841) / 10.18 / 11.22 / NS
LTB / 1419135_at / Lymphotoxin B (Ltb), mRNA / 12.64 / 9.49 / NS
CD79B / 1417640_at / CD79B antigen, mRNA (cDNA clone MGC:18588 IMAGE:4164551) / 10.50 / 8.56 / NS
MS4A4B / 1423467_at / Membrane-spanning 4-domains, subfamily A, member 4B, mRNA (cDNA clone MGC:41078 IMAGE:1248222) / 8.76 / 7.31 / NS
SELL / 1419480_at / Selectin, lymphocyte , mRNA / 9.02 / 7.29 / NS
SPIB / 1460407_at / Spi-B transcription factor (Spi-1/PU.1 related) (Spib), mRNA / 5.13 / 5.83 / NS
TCRB-J / 1426772_x_at / T-cell receptor beta, variable 13, mRNA (cDNA clone MGC:41373 IMAGE:1365841) / 5.83 / 5.50 / NS
PLA2G2D / 1454157_a_at / Non-secreted phospholipase A2 (Splash) mRNA, complete cds; nuclear gene for mitochondrial product / 5.98 / 5.13 / NS
BANK1 / 1456328_at / B-cell scaffold protein with ankyrin repeats 1 (Bank1), mRNA / 3.65 / 4.73 / NS
C3 / 1423954_at / Complement component 3 (C3), mRNA / 6.89 / 3.99 / NS
SERPINB1A / 1416318_at / EIA (Serpinb1) / 17.74 / 3.91 / NS
BLNK / 1451780_at / B-cell linker (Blnk), mRNA / 5.31 / 3.85 / NS
SERPINA3G / 1424923_at / Spi2 proteinase inhibitor (spi2/eb1) mRNA, 3' end / 5.20 / 3.62 / NS
TRDN / 1426144_x_at / PREDICTED: triadin [Mus musculus], mRNA sequence / 28.12 / 3.40 / NS
POU2AF1 / 1416957_at / POU domain, class 2, associating factor 1 (Pou2af1), mRNA / 4.29 / 3.16 / NS
RAC2 / 1417620_at / RAS-related C3 botulinum substrate 2, mRNA (cDNA clone MGC:6255 IMAGE:3495818) / 3.01 / 2.94 / NS
XLKD1 / 1429379_at / Extra cellular link domain-containing 1, mRNA (cDNA clone MGC:48275 IMAGE:3372151) / 7.86 / 2.71 / NS
H2-EB1 / 1417025_at / Histocompatibility 2, class II antigen E beta (H2-Eb1), mRNA / 3.46 / 2.69 / NS
VCAM1 / 1448162_at / Vascular cell adhesion molecule 1, mRNA (cDNA clone MGC:36028 IMAGE:4196681) / 4.43 / 2.62 / NS
LY6C / 1421571_a_at / Lymphocyte antigen 6 complex, locus C, mRNA (cDNA clone MGC:18546 IMAGE:4221342) / 3.81 / 2.56 / NS
F13A1 / 1448929_at / Coagulation factor XIII, A1 subunit (F13a1), mRNA / 3.77 / 2.52 / NS
H28 / 1425917_at / Histocompatibility 28, mRNA (cDNA clone MGC:28370 IMAGE:4020690) / 2.95 / 2.50 / NS
ANKRD1 / 1420991_at / Ankyrin repeat domain 1 (cardiac muscle), mRNA (cDNA clone MGC:46879 IMAGE:4953125) / 2.23 / 2.48 / NS
PTPRC / 1422124_a_at / Protein tyrosine phosphatase, receptor type, C (Ptprc), mRNA / 3.03 / 2.45 / NS
HRASLS3 / 1445597_s_at / BB404920 RIKEN full-length enriched, ES cells Mus musculus cDNA clone C330038H20 3' similar to X76453 R.norvegicus (Sprague Dawley) H-rev107 mRNA, mRNA sequence. / 4.67 / 2.42 / NS
CCR2 / 1421186_at / Chemokine (C-C motif) receptor 2 (Ccr2), mRNA / 2.54 / 2.33 / NS
CXCL9 / 1418652_at / Chemokine (C-X-C motif) ligand 9, mRNA (cDNA clone MGC:6179 IMAGE:3257716) / 3.03 / 2.23 / NS
CSF2RB1 / 1455660_at / Colony stimulating factor 2 receptor, beta 1, low-affinity (granulocyte-macrophage) (Csf2rb1), mRNA / 2.64 / 2.17 / NS
MS4A4C / 1450291_s_at / Membrane-spanning 4-domains, subfamily A, member 4C, mRNA (cDNA clone MGC:41005 IMAGE:1383416) / 2.58 / 2.14 / NS
IRF8 / 1416714_at / Interferon consensus sequence binding protein 1, mRNA (cDNA clone MGC:6194 IMAGE:3487214) / 3.02 / 2.14 / NS
H2-EA / 1452431_s_at / Histocompatibility 2, class II antigen A, alpha, mRNA (cDNA clone MGC:25391 IMAGE:3670758) / 2.56 / 2.11 / NS
ST8SIA4 / 1419186_a_at / ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4 (St8sia4), mRNA / 2.41 / 2.06 / NS
MS4A6C / 1450234_at / Membrane-spanning 4-domains, subfamily A, member 6C (Ms4a6c), mRNA / 2.46 / 2.03 / NS
CCL6 / 1417266_at / Chemokine (C-C motif) ligand 6, mRNA (cDNA clone MGC:6215 IMAGE:3492808) / 3.12 / 2.02 / NS
MSR1 / 1448061_at / Macrophage scavenger receptor 1 (Msr1), mRNA / 2.45 / 1.99 / NS
LY86 / 1422903_at / Lymphocyte antigen 86 (Ly86), mRNA / 3.13 / 1.97 / NS
CXCL12 / 1448823_at / Chemokine (C-X-C motif) ligand 12, transcript variant 1, mRNA (cDNA clone MGC:6119 IMAGE:3483088) / 2.96 / 1.92 / NS
CYBB / 1436778_at / Cytochrome b-245, beta polypeptide (Cybb), mRNA / 2.13 / 1.92 / NS
TRIM30 / 1451860_a_at / Tripartite motif protein 30, mRNA (cDNA clone MGC:6159 IMAGE:3493256) / 1.80 / 1.91 / NS
STK17B / 1450997_at / AV173139 Mus musculus liver C57BL/6J 14-day embryo Mus musculus cDNA clone 4432404O09, mRNA sequence. / 2.18 / 1.84 / NS
AIF1 / 1418204_s_at / Allograft inflammatory factor 1, mRNA (cDNA clone MGC:35939 IMAGE:5346103) / 2.72 / 1.84 / NS
IFI202B / 1452349_x_at / Interferon activated gene 202B, mRNA (cDNA clone MGC:18552 IMAGE:3990226) / 2.17 / 1.80 / NS
H2-AA / 1435290_x_at / Histocompatibility 2, class II antigen A, alpha, mRNA (cDNA clone MGC:25391 IMAGE:3670758) / 2.36 / 1.80 / NS
IFIT3 / 1449025_at / Interferon-induced protein with tetratricopeptide repeats 3, mRNA (cDNA clone MGC:6081 IMAGE:3487345) / 2.00 / 1.78 / NS
SCOTIN / 1423986_a_at / scotin gene / 1.77 / 1.76 / NS
KCTD12 / 1434881_s_at / MKIAA1778 protein / 2.94 / 1.76 / NS
CRBN / 1423094_at / Cereblon (Crbn), transcript variant 1, mRNA / 1.84 / 1.63 / NS
FCGR3 / 1448620_at / Fc gamma receptor III (Fcgr3) mRNA, Fcgr3-b allele / 2.18 / 1.59 / NS
ARHGAP29 / 1454745_at / Rho GTPase activating protein 29 (Arhgap29), mRNA / 2.00 / 1.59 / NS
FGL2 / 1421855_at / Fibrinogen-like protein 2, mRNA (cDNA clone MGC:19044 IMAGE:4189071) / 1.98 / 1.59 / NS
CD36 / 1423166_at / CD36 antigen, mRNA (cDNA clone MGC:6068 IMAGE:3481681) / 14.33 / 1.57 / NS
PLAC8 / 1451335_at / C15 protein / 1.90 / 1.56 / NS
PTPN18 / 1419125_at / Protein tyrosine phosphatase, non-receptor type 18, mRNA (cDNA clone MGC:5834 IMAGE:3591418) / 1.61 / 1.53 / NS
IFI203 / 1448775_at / Interferon activated gene 203 (Ifi203), mRNA / 1.68 / 1.53 / NS
FCGR2B / 1435477_s_at / Fc receptor, IgG, low affinity IIb, mRNA (cDNA clone MGC:46888 IMAGE:3993915) / 1.73 / 1.52 / NS
PMP22 / 1417133_at / Peripheral myelin protein, mRNA (cDNA clone MGC:18564 IMAGE:4225316) / 2.17 / 1.51 / NS
H2-T23 / 1449556_at / Histocompatibility 2, T region locus 23, mRNA (cDNA clone MGC:11529 IMAGE:3709267) / 1.48 / 1.45 / NS
IL2RG / 1416295_a_at / Interleukin 2 receptor, gamma chain, mRNA (cDNA clone MGC:25334 IMAGE:4924951) / 1.51 / 1.42 / NS
CYBA / 1454268_a_at / Cytochrome b-245, alpha polypeptide (Cyba), mRNA / 1.71 / 1.42 / NS
STAG2 / 1450396_at / Stromal antigen 2 (Stag2), mRNA / 2.00 / 1.36 / NS
SNX2 / 1460224_at / Sorting nexin 2, mRNA (cDNA clone MGC:6322 IMAGE:2812557) / 1.50 / 1.33 / NS
KTN1 / 1455434_a_at / Kinectin 1 (Ktn1), mRNA / 1.56 / 1.33 / NS
SYNJ2BP / 1417834_at / Activin receptor interacting protein 2 (Arip2) / 1.55 / 1.33 / NS
SPCS3 / 1451175_at / Signal peptidase complex subunit 3 homolog (S. cerevisiae), mRNA (cDNA clone MGC:62761 IMAGE:5375997) / 1.49 / 1.32 / NS
Supplemental Table 3. 11 genes down-regulated in tumors resected at estrus stage compared to tumors resected at diestrus stage© Genes are listed in descending order of EC vs. DC fold difference.
Gene Symbol / Sequence Code / Sequence Description / EC/DNC / EC/DC / DC/ DNCDNMT1 / 1447877_x_at / BB116018 RIKEN full-length enriched, adult male urinary bladder Mus musculus cDNA clone 9530053A16 3' similar to AF036007 Mus musculus DNA (cytosine-5)-methyltransferase (Dnmt1) mRNA, with oocyte-specific, mRNA sequence. / -1.79 / -1.59 / NS
SLCO2A1 / 1450032_at / Solute carrier organic anion transporter family, member 2a1, mRNA (cDNA clone MGC:7145 IMAGE:3158233) / -1.73 / -1.62 / NS
SLC7A1 / 1454992_at / Solute carrier family 7 (cationic amino acid transporter, y+ system), member 1 (Slc7a1), mRNA / -1.61 / -1.35 / NS
CEACAM1 / 1427630_x_at / CEA-related cell adhesion molecule 2, mRNA (cDNA clone MGC:18435 IMAGE:4241449) / -1.50 / -1.41 / NS
ITGA3 / 1455158_at / Integrin alpha 3 (Itga3), mRNA / -1.46 / -1.44 / NS
TRIO / 1433745_at / Triple functional domain (PTPRF interacting), mRNA (cDNA clone IMAGE:6306998) / -1.46 / -1.40 / NS
POGZ / 1455046_a_at / Pogo transposable element with ZNF domain, mRNA (cDNA clone IMAGE:5322259) / -1.45 / -1.33 / NS
AMPD2 / 1438941_x_at / Adenosine monophosphate deaminase 2 (isoform L) (Ampd2), mRNA / -1.44 / -1.33 / NS
NAV1 / 1436907_at / Importin9 isoform 1 (Importin9) / -1.41 / -1.33 / NS
CDH1 / 1448261_at / Cadherin 1 (Cdh1), mRNA / -1.41 / -1.31 / NS
BAT2D / 1429433_at / BAT2 domain containing 1, mRNA (cDNA clone IMAGE:5151726) / -1.36 / -1.34 / NS
1
Supplemental Table 4. Biologic functions of 90 estrous-cycling genes
1
Supplemental Table 5. 67 genes up-regulated in tumors associated with post-resection cure© Genes are listed in descending order of EC vs. DNC fold difference. EC: EC-pooled; DC: DC-pooled; DNC: DNC-pooled
Gene Symbol / Sequence Code / Sequence Description / EC/DNC / DC/DNCCmya5 / 1427312_at / Stretch response protein 553 (sr553 gene) / 9.68 / 10.93
Pgam2 / 1418373_at / Phosphoglycerate mutase 2, mRNA (cDNA clone MGC:18358 IMAGE:4235174) / 8.76 / 14.42
Cox8b / 1449218_at / Cytochrome c oxidase, subunit VIIIb, mRNA (cDNA clone MGC:41187 IMAGE:1515187) / 8.73 / 9.65
Mybpc2 / 1455736_at / Myosin binding protein C, fast-type (Mybpc2), mRNA / 8.67 / 6.58
Ckm / 1417614_at / Creatine kinase, muscle (Ckm), mRNA / 8.22 / 8.50
Cox6a2 / 1457633_x_at / AV005759 Mus musculus C57BL/6J heart Mus musculus cDNA clone 1010001P05, mRNA sequence. / 7.66 / 13.62
Myl1 / 1452651_a_at / Myosin, light polypeptide 1 (Myl1), mRNA / 6.35 / 5.89
Myh4 / 1427026_at / Myosin heavy chain IIB (MYHC-IIB gene) / 6.21 / 7.90
Tnni2 / 1416889_at / Troponin I, skeletal, fast 2, mRNA (cDNA clone MGC:41207 IMAGE:1448494) / 5.99 / 7.50
Bex1 / 1448595_a_at / Brain expressed X-linked protein 1 (Bex1) / 5.89 / 4.38
Pygm / 1448602_at / Muscle glycogen phosphorylase, mRNA (cDNA clone MGC:13713 IMAGE:3989941) / 5.74 / 10.54
Tnnc2 / 1417464_at / Troponin C2, fast, mRNA (cDNA clone MGC:36164 IMAGE:5026329) / 5.52 / 7.17
Tnnt3 / 1450118_a_at / Troponin T3, skeletal, fast, mRNA (cDNA clone MGC:5854 IMAGE:3594256) / 5.43 / 6.90
Actn3 / 1418677_at / Actinin alpha 3 (Actn3), mRNA / 5.36 / 5.28
Ccl12 / 1419282_at / Chemokine (C-C motif) ligand 12, mRNA (cDNA clone MGC:41146 IMAGE:1548072) / 5.31 / 3.15
Sh3bgr / 1422644_at / Putative SH3BGR protein (SH3BGR gene) / 4.96 / 5.56
Sfrp2 / 1448201_at / Secreted frizzled-related sequence protein 2, mRNA (cDNA clone MGC:25299 IMAGE:4487469) / 4.67 / 5.05
Fxyd1 / 1421374_a_at / FXYD domain-containing ion transport regulator 1, transcript variant 5, mRNA (cDNA clone MGC:19114 IMAGE:4208962) / 4.63 / 4.51
Mef2c / 1424852_at / Myocyte enhancer factor 2C, mRNA (cDNA clone MGC:46981 IMAGE:4500786) / 4.41 / 4.22
Ankrd23 / 1428183_at / Ankyrin repeat domain 23 (Ankrd23), mRNA / 4.31 / 3.23
Tcap / 1423145_a_at / Titin-cap, mRNA (cDNA clone MGC:41137 IMAGE:1314541) / 4.29 / 7.00
Neb / 1435355_at / PREDICTED: nebulin [Mus musculus], mRNA sequence / 4.28 / 4.28
Ppp1r3c / 1433691_at / Protein phosphatase 1, regulatory (inhibitor) subunit 3C (Ppp1r3c), mRNA / 4.20 / 7.56
Smpx / 1418095_at / SMPX protein (Smpx) / 4.16 / 3.54
Eno3 / 1417951_at / Enolase 3, beta muscle, mRNA (cDNA clone MGC:18648 IMAGE:4217678) / 3.99 / 4.49
Art1 / 1451372_a_at / ADP-ribosyltransferase 1 (Art1), mRNA / 3.92 / 3.43
Myf6 / 1419150_at / Myogenic factor 6 (Myf6), mRNA / 3.51 / 3.24
Atp2a1 / 1419312_at / ATPase, Ca++ transporting, cardiac muscle, fast twitch 1, mRNA (cDNA clone MGC:28518 IMAGE:4191741) / 3.48 / 6.89
Des / 1426731_at / Desmin, mRNA (cDNA clone MGC:25641 IMAGE:4219280) / 3.37 / 2.81
Pdlim3 / 1449178_at / PDZ and LIM domain 3 (Pdlim3), mRNA / 3.32 / 2.53
Myom2 / 1450917_at / Myomesin 2 (Myom2), mRNA / 2.98 / 5.35
Pdk4 / 1417273_at / Pyruvate dehydrogenase kinase, isoenzyme 4, mRNA (cDNA clone MGC:13840 IMAGE:4188580) / 2.78 / 2.94
Gpx3 / 1449106_at / Glutathione peroxidase 3 (Gpx3), mRNA / 2.74 / 1.92
Acta1 / 1427735_a_at / Actin, alpha 1, skeletal muscle, mRNA (cDNA clone MGC:18923 IMAGE:3587657) / 2.69 / 3.66
Fhl1 / 1417872_at / Four and a half LIM domains 1, mRNA (cDNA clone MGC:32284 IMAGE:5012394) / 2.62 / 2.56
Ndrg2 / 1448154_at / N-myc downstream regulated gene 2, mRNA (cDNA clone MGC:13746 IMAGE:4211421) / 2.57 / 2.99
Fbn1 / 1460208_at / Fibrillin 1, mRNA (cDNA clone IMAGE:3483994) / 2.56 / 2.01
Cox7a1 / 1418709_at / Cytochrome c oxidase, subunit VIIa 1 (Cox7a1), mRNA / 2.50 / 4.82
Hspb7 / 1434927_at / Heat shock protein family, member 7 (cardiovascular) (Hspb7), mRNA / 2.43 / 2.59
Loxl1 / 1451978_at / Lysyl oxidase-like 1, mRNA (cDNA clone MGC:46960 IMAGE:5251968) / 2.38 / 2.17
Ttn / 1427446_s_at / Titin, mRNA (cDNA clone IMAGE:5149168) / 2.23 / 4.22
Ugp2 / 1426461_at / UDP-glucose pyrophosphorylase 2, mRNA (cDNA clone MGC:38555 IMAGE:5353993) / 2.22 / 1.95
Col3a1 / 1427884_at / Procollagen, type III, alpha 1, mRNA (cDNA clone IMAGE:3497583) / 2.19 / 2.08
Atp1a2 / 1452308_a_at / ATPase, Na+/K+ transporting, alpha 2 polypeptide, mRNA (cDNA clone MGC:36347 IMAGE:4955003) / 2.17 / 2.38
Ryr1 / 1427306_at / RyR1 mRNA for skeletal muscle ryanodine receptor / 2.12 / 3.87
Ubqln2 / 1450021_at / Ubiquilin 2, mRNA (cDNA clone IMAGE:4009660) / 2.10 / 1.88
Maf / 1456060_at / Avian musculoaponeurotic fibrosarcoma (v-maf) AS42 oncogene homolog (Maf), mRNA / 1.99 / 1.56
Lox / 1416121_at / Lysyl oxidase, mRNA (cDNA clone MGC:11525 IMAGE:2655752) / 1.97 / 1.72
Grb10 / 1425458_a_at / Growth factor receptor bound protein 10, mRNA (cDNA clone MGC:28740 IMAGE:4481345) / 1.96 / 2.08
Tpm2 / 1449577_x_at / Tropomyosin 2, beta, mRNA (cDNA clone MGC:35840 IMAGE:4990394) / 1.92 / 3.28
Col1a2 / 1423110_at / Procollagen, type I, alpha 2, mRNA (cDNA clone IMAGE:3481648) / 1.88 / 2.13
Ctla2a / 1416811_s_at / Cytotoxic T lymphocyte-associated protein 2 alpha, mRNA (cDNA clone MGC:41186 IMAGE:1244311) / 1.82 / 1.57
Csf1r / 1419872_at / Colony stimulating factor 1 receptor (Csf1r), mRNA / 1.77 / 1.50
S100a9 / 1448756_at / S100 calcium binding protein A9 (calgranulin B), mRNA (cDNA clone MGC:41215 IMAGE:1332797) / 1.74 / 1.48
Myh1 / 1427868_x_at / Myosin heavy chain IIX (MYHC-IIX gene) / 1.72 / 11.38
Cxcl4 / 1448995_at / Chemokine (C-X-C motif) ligand 4 (Cxcl4), mRNA / 1.70 / 1.84
C1qb / 1437726_x_at / Complement component 1, q subcomponent, beta polypeptide (C1qb), mRNA / 1.69 / 1.38
Timp2 / 1460287_at / Tissue inhibitor of metalloproteinase 2 (Timp2), mRNA / 1.68 / 1.40
Sparc / 1448392_at / Secreted acidic cysteine rich glycoprotein, mRNA (cDNA clone MGC:6232 IMAGE:3586402) / 1.66 / 1.66
Serpinf1 / 1416168_at / Serine (or cysteine) peptidase inhibitor, clade F, member 1, mRNA (cDNA clone MGC:29977 IMAGE:5123884) / 1.62 / 1.56
Pcolce / 1437165_a_at / Procollagen C-endopeptidase enhancer protein (Pcolce), mRNA / 1.60 / 1.68
Gyg / 1459522_s_at / Glycogenin 1, mRNA (cDNA clone MGC:35999 IMAGE:4487769) / 1.59 / 1.50
Igfbp7 / 1423584_at / Polymerase (RNA) II (DNA directed) polypeptide B (Polr2b), mRNA / 1.56 / 1.32
Socs3 / 1455899_x_at / BB241535 RIKEN full-length enriched, 3 days neonate thymus Mus musculus cDNA clone A630087F01 3' similar to U88328 Mus musculus suppressor of cytokine signalling-3 (SOCS-3) mRNA, mRNA sequence. / 1.51 / 1.42
Hist1h2bp / 1452540_a_at / Histone H2b mRNA, 3' end / 1.49 / 1.61
Cryab / 1434369_a_at / Crystallin, alpha B, mRNA (cDNA clone MGC:18591 IMAGE:4238233) / 1.44 / 1.82
Ube2b / 1423106_at / Ubiquitin-conjugating enzyme E2B, RAD6 homology (S. cerevisiae) (Ube2b), mRNA / 1.37 / 1.37
Supplemental Table 6. 2 genes down-regulated in tumors associated with post-resection cure© Genes are listed in descending order of EC vs. DNC fold difference. EC: EC-pooled; DC: DC-pooled; DNC: DNC-pooled
Gene Symbol / Sequence Code / Sequence Description / EC/DNC / DC/DNCTrib3 / 1456225_x_at / Tribbles homolog 3 (Drosophila) (Trib3), transcript variant 1, mRNA / -1.49 / -1.44
Cxcl1 / 1419209_at / Chemokine (C-X-C motif) ligand 1 (Cxcl1), mRNA / -1.43 / -1.73
1
Supplemental Table 7. Biologic functions of 69 cure-associated genes
1
Supplemental Table 8. 24 estrous-cycling &and cure-associated genes © All 24 genes have EC>DC>DNC relationship. Genes are listed in descending order of EC vs. DNC fold difference. EC: EC-pooled; DC: DC-pooled; DNC: DNC-pooled
Gene Symbol / Sequence Code / Sequence Description / EC/ DNC / EC/DC / DC/ DNCIgf1 / 1419519_at / Insulin-like growth factor 1, mRNA (cDNA clone MGC:18617 IMAGE:4194295) / 12.90 / 2.79 / 4.93
Fabp4 / 1417023_a_at / Fatty acid binding protein 4, adipocyte (Fabp4), mRNA / 6.40 / 3.44 / 1.86
Car3 / 1449434_at / Carbonic anhydrase 3, mRNA (cDNA clone MGC:18583 IMAGE:4195712) / 5.90 / 1.32 / 4.48
Lpl / 1415904_at / Lipoprotein lipase, mRNA (cDNA clone MGC:5666 IMAGE:3584788) / 5.65 / 1.66 / 3.40
Cugbp2 / 1423895_a_at / Hypothetical protein B230218O03 (B230218O03), mRNA / 4.47 / 1.56 / 2.86
Ifi27 / 1426278_at / Interferon stimulated gene 12 (Isg12) / 4.29 / 2.73 / 1.57
Ms4a6b / 1439956_at / Membrane-spanning 4-domains, subfamily A, member 6B, mRNA (cDNA clone MGC:35625 IMAGE:3984299) / 3.85 / 2.65 / 1.45
Mrc1 / 1450430_at / Mannose receptor, C type 1 (Mrc1), mRNA / 3.74 / 2.04 / 1.83
Ccl9 / 1448898_at / Strain SJL/J small inducible cytokine A10 (ScyA10) / 3.53 / 2.07 / 1.70
Iigp1 / 1419043_a_at / Interferon inducible GTPase 1, mRNA (cDNA clone MGC:5713 IMAGE:3483102) / 3.10 / 1.93 / 1.61
Lyzs / 1423547_at / Lysozyme, mRNA (cDNA clone IMAGE:2655292) / 3.01 / 1.77 / 1.70
Cd74 / 1425519_a_at / Ia-associated invariant chain, mRNA (cDNA clone MGC:6517 IMAGE:2650401) / 2.99 / 2.12 / 1.41
Glul / 1426236_a_at / Glutamine synthetase (Glul) / 2.84 / 1.60 / 1.77
Asb5 / 1449356_at / Ankyrin repeat and SOCs box-containing protein 5 (Asb5), mRNA / 2.81 / 1.64 / 1.72
Laptm5 / 1436905_x_at / Lysosomal-associated protein transmembrane 5, mRNA (cDNA clone MGC:13988 IMAGE:4037686) / 2.62 / 1.82 / 1.44
Ccl8 / 1419684_at / Chemokine (C-C motif) ligand 8 (Ccl8), mRNA / 2.57 / 1.57 / 1.63
Ms4a6d / 1419599_s_at / Membrane-spanning 4-domains, subfamily A, member 6D, mRNA (cDNA clone MGC:25778 IMAGE:4016611) / 2.55 / 1.67 / 1.53
Trpt1 / 1456014_s_at / TRNA phosphotransferase 1 (Trpt1), mRNA / 2.43 / 1.56 / 1.56
Sepp1 / 1452141_a_at / Selenoprotein P, plasma, 1, mRNA (cDNA clone MGC:5722 IMAGE:3486761) / 2.41 / 1.72 / 1.40
Serping1 / 1416625_at / Serine (or cysteine) peptidase inhibitor, clade G, member 1, mRNA (cDNA clone MGC:5908 IMAGE:3485810) / 2.39 / 1.51 / 1.59
Postn / 1423606_at / Periostin, osteoblast specific factor, mRNA (cDNA clone MGC:25368 IMAGE:4457222) / 2.28 / 1.55 / 1.47
Ctla2b / 1452352_at / Cytotoxic T lymphocyte-associated protein 2 alpha, mRNA (cDNA clone MGC:41186 IMAGE:1244311) / 2.19 / 1.49 / 1.47
Rgs5 / 1417466_at / Mus musculus regulator of G-protein signaling 5 (Rgs5), mRNA. / 1.99 / 1.41 / 1.41
C1qa / 1417381_at / Complement component 1, q subcomponent, alpha polypeptide, mRNA (cDNA clone MGC:6262 IMAGE:3592169) / 1.90 / 1.37 / 1.39
1
1