Texas Branch

American Association for

Laboratory Animal Science

MICRO SCIENCE

20

Meeting Program

February 17-19, 2016

Sheraton Hotel

Arlington, Texas

www.tbaalas.net

20

Texas Branch AALAS Board & Committee Members

President Jamie Greaver, BS, RLATG

Past President Angie Hitt, RLATG

President Elect Lindsay Holmes, BS, RLAT

Secretary Karen Guerra, MBA, CMAR, RLATG

Treasurer Brian Gibson, DVM

Board Member Ryan Byrd, RLAT

Board Member Adrienne Ferguson Duran

Board Member Elizabeth Magden, DVM, MS, DACLAM,

Board Member Chris Southern

District VII Trustee Karen Guerra, MBA, CMAR, RLATG

District VII Trustee Teresa Neubauer, CMAR, RLATG

Alt D7 Trustee Toni Mufford

Commerical Liaison Chris Rogers, Envigo

Commercial Co-chair John Eppes

Parliamentarian T. Lane Watkins, CMAR, RLATG

Historian Co-chair Brian Gillman, RLATG

Legislative Nicole Monts De Oca, DVM

Membership Chair Belinda Proctor, RLATG

Membership Co-chair Jenni Adams

Nominations/Elections Steve Sterle, LATG

Publications Chair OPEN

Publications Co-chair Carrie Schultz, PhD

Technician Branch Rep Summer Boyd, MS, RVT, RLATG

Technician Branch Co-chair Eli Rodriguez, LATG

Long Range Planning Angie Hitt

Education & Training Chair Sheri Leavitt, BS, CMAR, RLATG

Education & T. Co-chair Tressie Roark, LVT, LATG

ALL Coordinator Ashley Pawelka

Webmaster Chair Cordelia Rasa

Webmaster Co-chair David Disselhorst & Michelle Wodzak

Metro Coordinator Sheri Leavitt Galveston

Metro Coordinator Michelle Johnson Houston

Metro Coordinator Jack Simons, MS, RLATG San Antonio

Metro Coordinator Gabby Kapp, CMAR Central

Metro Coordinator Katrina Donelson, RLAT Dallas / Fort Worth

Metro Coordinator Oscar Sanchez, LATG West Texas

Metro Coordinator Velvet Lee Finckbone, MS, RLAT Panhandle

2016 Meeting Planner Paula Rigling

2016 Program Co-chairs Tressie Roark, LVT, LATG & Leticia McGuffy, ALAT

2016 Local Arrangements Igor Smiljcic, RLAT & Michelle Wodzak, RVT, RLATG

2016 Awards Chair Pamela Huskey CMAR, RLATG

2016 Awards Co-chair Amanda Trimble

2016 Sponsorships Becky Blackwood, DVM, DACLAM & Jody Swain, MS, DVM

2016 Poster Coordinator Julie Roller, MS, CMAR, RLATG

2016 Silent Auction Ryan Byrd, RLAT & Elizabeth Magden, DVM, MS, DACLAM

2016 Tech Olympics Summer Boyd & Eli Rodriguez

2016 Meeting Logo Rico Cabuco

Congratulations to the new 2016-2017 Board

Schedule of Events

Wednesday: February 17, 2016

8:00-10:00 Set up in the vendor exhibit area

9:00-5:00 Registration

9:00-1:00 AREA Program

10:00-12:00 Exhibit Hall Open (closed 12:00-1:00 for lunch break)

10:00-12:00 Silent Auction Bidding

10:00-12:00 Battle of the Baskets

10:00-11:45 Vendor Presentations

11:45-12:45 Lunch Break

12:45-5:00 Opening Session/ Scientific Session

1:00-5:00 Battle of the Baskets

1:00-5:00 Exhibit Hall Open

1:00-5:30 Silent Auction Bidding

1:00-5:00 Poster Session

(3:00 - 4:00 Authors present at poster display for judging)

3:00-3:30 Break

5:00-9:00 Welcome Reception (Hors douevers & cash bar)

5:00 SOCIAL EVENT - Technician Olympics - Karaoke

Thursday: February 18, 2016

7:30-9:00 Continental Breakfast in Vendor Exhibit Hall

7:30-5:00 Exhibit Hall Open (closed 10:30-11:30 & 12:00-2:00)

8:00-4:30 Registration

8:00-10:00 Scientific Session

8:00-10:30 Silent Auction Bidding

8:00-12:00 Battle of the Baskets

10:00-10:30 Break

10:30-11:30 KEYNOTE SPEAKER

11:30-12:00 Break

11:30-12:00 Exhibit Hall Open

11:30-12:00 Silent Auction Bidding

12:00-2:00 Awards Banquet & Lucheon (lunch provided)

2:00-2:30 Break

2:00-5:00 Exhibit Hall Open

2:00-4:00 Silent Auction Bidding

2:30-3:00 Scientific Session

3:00-4:00 Round Table Discussion

4:00 Closing Remarks

4:00 Silent Auction Closes, Collect items at 4:15

Friday: February 19, 2016

7:30-9:00 Continental Breakfast

8:00-9:00 Registration

9:00-12:00 Leadership Training - Management 101

9:00-1:00 Necropsy Workshop off site at UT Arlington

12:00-2:00 TBAALAS Board Meeting

Scientific Session List of Speakers:

Wednesday: February 17, 2016

Moderator- Lindsay Holmes (Hall of Fame )

10:00 Vendor Andrea Gay Nesting 101: What's all the fuss

10:15 Vendor Denise Giuvelis Refinement of Pain Management Techniques

10:30 Vendor Rusty Thomas Caging Standards for Macaques - Euro vs US

10:45 Vendor Paul Lorcheim Pinworm Egg Inactivation Using Chlorine Dioxide Gas

11:00 Vendor Karena Thek Developing an Animal Care Plan for a Non-typical Species

11:15 Vendor John Zapata Rolling Along; Tips for Getting the Right Wheel and Caster

11:30 Vendor Rick Myer Pending- TBA

Moderator- Cordelia Rasa (Hall of Fame )

12:45 Opening Remarks Jamie Greaver, 2015 TBAALAS President

1:00 Laura Conour AALAS Branches and National AALAS: Bridging the Gap!

1:30 Pat Sikes Overview of the AALAS Foundation

1:45 Linnea Morley Providencia rettgeri in a Rhesus macaque

2:00 Kathryn Cavanaugh Early Pregnancy Determination and Daily Observations of Fetal Development

2:15 Keely McGrew Easy Does It: Effects of Caretaker Behavior on Lab Animal Performance

2:30 Michelle Wodzak Cardiovascular Disease Animal Model Development: the ups and downs

2:45 Courtney Sands We Have a Trick Up Our Sleeve: Increasing the Human-Animal Bond

3:00 Break

3:30 Lindsay Holmes Pigeons - Breeding, Training, Homing

3:45 Leticia McGuffey Replace, Reduce, Refine and the 4th R: Retirement

4:00 Elizabeth Magden Positive Reinforcement Training and Health Care in Nonhuman Primates

Thursday: February 18, 2016

Moderator- Sheri Leavitt (Hall of Fame )

8:15 Cindy Buckmaster Speak Now or Forever Rest in Peace!

9:15 Jamie Greaver Ergonomics or Else.....

9:45 Brian Smith Hepatic Lipidosis in a Rhesus macaque

10:00 Break

10:30-11:30 KEYNOTE SPEAKER – Sponsored by Tecniplast

11:30-12:00 Break

12:00-2:00 Awards Banquet

2:00-2:30 Break

Moderator- Shari Hunt (Hall of Fame )

2:30 Elizabeth Magden Use of an Implantable Loop Recorder in a Chimpanzee

3:00 Round Table How to Advance Your Career

4:00 Closing Remarks

Friday: February 19, 2016

WETLAB

9:00-1:00 Necropsy of Mouse and Rat Dr. Mary-Wight Carter, off site at UT Arlington

LEADERSHIP TRAINING (Super Bowl I & II)

Management 101 Class Agenda

9:00-9:30 Ice Breaker - Be A Leader Julie Wood

9:30-10:30 Difficult Conversations Julie Roller

10:30-10:45 Break

10:45-12:00 Handling Challenging Situations Frankie Howell/ Wood/Roller

Vendor Abstracts: (Wednesday, February 17, 2016) Hall of Fame

Andrea Gay, The Andersons Bedding Products - Nesting 101: What's All The Fuss

At a growing pace, more studies are being conducted with a focus on nesting, the behaviors associated with nesting and the many benefits the inclusion of nesting material can provide to both the animal and to the research. Does your facility use nesting material? What are the advantages of providing nesting material? If your facility doesn't use nesting material, why not? Is your facility aware of what nesting materials are available in today's market?

This presentation will discuss the role that nesting plays in today's research environment, the impact that nesting material has on cage environment, and the many options the market offers in the way of nesting material.

Denise Giuvelis, Clear H2O - Refinement of Pain Management Techniques in Laboratory Rodents

Pain management in laboratory rodents is challenging due to repetitive delivery methods, institutional compliance and animal welfare. The purpose of this study was to determine if carprofen could be effectively delivered to rodents using a dietary gel technology (MediGel CPF). Two experiments were conducted; ovariectomy on C57BL6 female mice in Dr. Edward Bilsky's lab at the University of New England and plantar-incision on male SD rats in Dr. Cholawat Pacharinska's lab at Stanford University. These studies assessed behavioral endpoints as well as blood plasma concentrations of rodents consuming MediGel CPF to those receiving a standard of care subcutaneous carprofen injection. In mice, similar reductions in abdominal sensitivities and plasma levels were observed in both treatment groups. In rats, tactile hypersensitivity was attenuated following surgery and plasma levels remained at a consistent level throughout treatment. MediGel CPF showed a good palatability in both mice and rats allowing caprofen concentrations in the blood to reach efficacious levels. The results from these studies allowed us to conclude that MediGel CPF represents a labor and cost effective as well as a less stressful refinement to traditional pain management techniques in rodents.

Rusty Thomas, Britz & Co. - Caging Standards for Macaques – Euro Standards vs US Standards

The European standards (ETS 123) in general require more cubic space for animals based on their age, not necessarily their size (weight/height). The US standards (Animal Welfare Act and the Guide for the Care and Use of Laboratory Animals) dictate specific cage sizes based generally on the weight and height of the animals.

Paul Lorcheim, Clordis - Pinworm Egg Inactivation Using Chlorine Dioxide Gas

Chlorine dioxide gas has been validated to be effective against pinworm eggs in a controlled laboratory setting. This presentation give a brief overview of that study, and include the properties that allow chlorine dioxide gas to be effective at eradicating pinworm eggs in the real world. Finally, this presentation will discuss various decontamination service projects where pinworms have been eradicated from a facility successfully.

Karena Thek, Bio-Serv - Developing an Animal Care Plan for a Non-Typical Lab Animal Species:

The Woodchuck (Marmota monax)

Have you ever got the news that a non-lab animal typical species would be arriving at your facility? How are you going to house them? What are you going to feed them? How do you enrich them? Developing an animal care program for a species you never worked with can be challenging and stressful. This presentation gives pointers on how you can develop your plan while working through an example: the Woodchuck. Learn interesting facts about woodchuck behavior, enrichment examples and challenges you may face in housing these unique creatures.

John Zapata, Ancare Corporation - Rolling Along; Tips for Getting the Right Wheel and Caster

Using proper terminology to convey accurate information to your sales rep can ensure you obtain the right caster or related parts. This presentation will introduce the participant to the proper names of the parts that make up a basic caster assembly. In additions, you will be introduced to a couple of different types of casters and wheels you should be familiar with. Tips will be shared on how to express the correct information such as with bolt patterns, wheel size and type of caster, saving time, frustration and money. The presentation will lead to some simple solutions for the care of your wheels and casters that you should be familiar with and will help you determine whether you need to replace the entire caster or just a component of the assembly. The intent of this presentation is for you to be comfortable knowing what exactly you need to keep you rolling!

Rick Myer, Superior Laboratory Services Inc. - TBA

Platform Abstracts: (Wednesday, February 17, 2016) Hall of Fame

^ denotes award eligible *denotes primary presenter ~denotes first time presenter

AALAS Branches and National AALAS: Bridging the Gap!

Laura Conour, DVM

Princeton University & 2016 National AALAS President

My platform for my year as AALAS President is to explore how our community can bridge the gap between the local AALAS branches and the national AALAS association as a whole. Grassroots organizations and the network they compose on local and regional levels strengthen our laboratory animal community and permit education, outreach and networking across all geographic areas. As part of this presentation, I’ll discuss operations, financial management, and constituency of the branches and what services national AALAS currently provides to the branches. I would like to open a dialogue for us, as a group to discuss the following questions:

· How might the branches and national AALAS benefit from a closer, more solidified relationship?

· What could the branches do to further support national AALAS?

· What can national AALAS do to provide services to the branches in order to foster a grassroots initiative in support of our mission to advance responsible laboratory animal care and use to benefit people and animals?

Overview of the AALAS Foundation - What it does and how you can help.

Pat Sikes, MS

Charles River Laboratories

The AALAS Foundation is a non-profit organization separate from National AALAS. It relies on donations to bring awareness to the general public about the compassionate professionals working in the field of laboratory animal science, communicate the important role of animals in research and offer free resource materials to assist laboratory animal science professionals conducting outreach activities in their local communities.

Providencia rettgeri in a Rhesus macaque (Macaca mulatta)

Linnea Morley, DVM* J. Goldman

University of Texas Health Science Center - Houston

Providencia rettgeri is a gram negative bacterium in the family Enterobacteriaceae. It has been found in water and land environments and is a cause of traveler's diarrhea. It is a common urinary tract pathogen and a source of nosocomial infections in humans. Some isolates of Providencia rettgeri have been found to be NDM (New Delhi Metallo-Beta-Lactamase) positive, resulting in an antibiotic resistant strain. Providencia rettgeri was cultured during a cranial explant in a 9 year old intact male rhesus macaque with a history of allergies. Treatment with Enrofloxacin was met with an allergic reaction, and the remainder of the treatment was continued with Gentamicin. The remaining cranial implant is scheduled for explant in November 2015, at which time another culture will be performed to determine if the above treatment was successful. This report documents the first known case of Providencia rettgeri associated with an infection at the site of a cranial implant in a rhesus macaque (Macaca mulatta).

EarlyPregnancy Determination and Daily Observations of Fetal Development via Ultrasonography in Dutch Belted Rabbits

^~Kathryn Cavanaugh, BS* E. Rose, G. Gum, S. Pritt

UT Southwestern Medical Center - Dallas

Palpation has been the predominant method of pregnancy determination in rabbits, typically practiced 10-14 days post copulation (PC). By using ultrasonography, producers may detect pregnancy significantly sooner. A sample size of N=21 does were evaluated to determine the earliest date of pregnancy detection by ultrasound and to chronicle fetal development. Group 1 (n=11) was evaluated daily from day 7 PC through day 28 Group 2 (n=10) was evaluated from day 7 PC through day 28, with 3 does also being evaluated on day 5 and 6 PC. Day 5 PC images exhibited clearly visible vesicles in 2 out of 3 does. For all does evaluated on day 6, there was 100% pregnancy confirmation. Day 6 PC vesicles measured an average size of 040 cm in diameter, increasing to an average of 055 cm in diameter by day 7 PC. Images were captured daily until day 28 PC. Clear images were documented in an attempt to make ultrasonography a more accessible method of pregnancy determination and fetal health evaluation. This report demonstrates that pregnancy can be determined as early as day 5 PC, and reliably at day 6 PC, as opposed to previous reports that stated the earliest date for pregnancy detection was day 7 PC.