Supplementary Table 1. Full list of phosphopeptides and phosphoproteins identified from human pituitary tissue by in-gel IEF-LC-MS/MS.

No / Protein Name / SWISS-PROT ID /

Identified Peptide

/ aSite / bReprodu-cibility / Best Xcorr
-charge
1 / Secretogranin I / P05060 / (R) GEDS*SEEKHLEEPGETQNAFLNER / 182 (183) / 3 / 5.79 - 3
(R) GEDS*S*EEKHLEEPGETQNAFLNER
(K)GERGEDS*S*EEKHLEEPGETQNAFLNER
(R)GEDS*S*EEKHLEEPGETQNAF / 182, 183 / 3
3
1 / 5.04 - 3
3.72 – 3
2.96 - 2
(R)ADEPQWSLYPSDSQVS*EEVK
(R)ERADEPQWSLYPSDSQVS*EEVK / 149 / 2
1 / 3.67 - 2
4.27 - 3
(K)S*AEFPDFYDSEEPVSTHQEAENEKDR / 617 / 2 / 3.60 - 3
(K)SAEFPDFYDS*EEPVSTHQEAENEK / 626 / 1 / 3.15 – 3
(R)APRPQS*EESWDEEDKR / 377 (380) / 2 / 2.73 - 3
(R)AS*EEEPEYGEEIK / 335 / 2 / 2.51 - 2
(K)MAHGYGEES*EEER
(K)M#AHGYGEES*EEER
(K)MAHGYGEES*EEERGLEPGK / 405 / 3
1
2 / 2.58 – 2
1.92 – 2
2.66 - 2
(K)GHPQEES*EESNVSMASLGEK
(K)GHPQEES*EESNVSMASLGE / 311 / 2
1 / 3.24 - 2
3.26 - 2
(R)S*QEES*EEGEEDATSEVDK
(R)S*QEES*EEGEEDATSEVDKR / 259, 263 / 1
1 / 2.65 – 2
2.53 - 2
2 / Somatotropin / P01241 / (K)FDTNS*HNDDALLK / 176 / 3 / 3.87 - 2
(N)SLVYGAS*DSNVYDLLK
(F)ANSLVYGAS*DSNVYDLLK / 132 / 3
1 / 4.23 - 2
3.47 - 2
3 / Secretogranin II / P13521 / (R)VPGQGS*SEDDLQEEEQIEQAIK / 532 (533) / 3 / 4.51 - 2
(K)RVPGQGSS*EDDLQEEEQIEQAIK / 533 (532) / 2 / 2.74 - 2
4 / Chromogranin A / P10645 / (K)GEQEHS*QQKEEEEEMAVVPQGLFR
(K)GEQEHS*QQKEEEEEM#AVVPQGLFR / 300 / 2
3 / 3.28 – 3
4.04 - 3
(R)GGKS*GELEQEEER / 322 / 3 / 3.69 - 2
(R)GGKS*GELEQEEERLS*KEWEDS
(K)S*GELEQEEERLS*KEWEDS
(R)GGKS*GELEQEEERLS*KEWED
(R)GGKS*GELEQEEERLS*KEWEDSK
(K)S*GELEQEEERLS*KEWEDSKR / 322, 333 / 1
1
1
1
1 / 2.64 - 3
3.47 - 3
2.87 – 3
2.65 – 3
2.25 - 3
(R)EDS*KEAEKS*GEATDGARPQALPEPMQESK / 136, 142 / 2 / 2.99 - 3
(K)HSGFEDELSEVLENQS*SQAELK / 112 (113) / 1 / 3.94 - 2
(K)HSGFEDELSEVLENQSS*Q / 113 (112) / 2 / 3.77 - 2
(K)YPGPQAEGDS*EGLSQGLVDR / 203 / 1 / 3.12 - 2
(E)EDS*LEAGLPLQVR / 402 / 3 / 3.28 - 2
5 / Galanin / P22466 / (R)LLDLPAAASS*EDIER
(R)LLDLPAAASS*EDIE / 117 (118) / 3
3 / 4.36 – 2
3.96 - 2
6 / 60S Acidic Ribosomal Protein P1 and/or P2 / P05386/P05387 / (K)KEESEES*DDDMGFGLFD
(K)KEESEES*DDDM#GFGLFD / 104 / 3
1 / 3.79 – 2
3.58 - 2
(K)KEES*EESDDDMGFGLFD
(K)KEES*EESDDDM#GFGLFD
(K)VEAKKEES*EESDDDM#GFGLF / 102 / 2
2
1 / 3.02 - 2
3.90 - 2
2.99- 2
(K)KEES*EES*DDDMGFGLFD
(K)KEES*EES*DDDM#GFGLFD / 102, 104 / 3
2 / 3.44 – 2
2.76 - 2
7 / Telomerase-Binding Protein p23 / Q15185 / (K)DWEDDS*DEDMSNFDR
(K)DWEDDS*DEDM#SNFDR / 113 / 3
2 / 4.13 – 2
3.61 - 2
8 / Corticotropin-Lipotropin (POMC) / P01189 / (K)VYPNGAEDES*AEAFPLEF
(K)VYPNGAEDES*AEAFPLEFK
(N)GAEDES*AEAFPLEF
(K)VYPNGAEDES*AEAFPLE / 168 / 1
3
1
1 / 2.09 - 2
3.59 - 2
3.50 - 2
2.61 - 2
9 / Alpha-1 Catenin / P35221 / (R)TPEELDDS*DFETEDFDVR / 641 / 1 / 4.15 - 2
10 / cAMP- Dependent Protein Kinase Type II-Alpha Regulatory Chain / P13861 / (K)GDS*ES*EEDEDLEVPVPSR / 77, 79 / 1 / 3.36 - 2
11 / Elongation Factor 1-delta / P29692 / (K)KPATPAEDDEDDDIDLFGS*DNEEEDKEAAQLR / 162 / 2 / 5.41 - 3
12 / Hepatoma-Derived Growth Factor (HDGF) / P51858 / (K)GNAEGS*S*DEEGKLVIDEPAK / 132, 133 / 1 / 3.43 - 2
13 / Triosephosphate Isomerase / P60174 / (R)KQS*LGELIGTLNAAK / 20 / 1 / 2.90 - 2
14 / 3-Phosphoinositide Dependent Protein Kinase-1 / O15530 / (R)ANS*FVGTAQYVSPELLTEK / 241 / 1 / 4.25 - 2
15 / 60S Acidic Ribosomal Protein P0 / P05388 / (K)VEAKEES*EESDEDMGFGLFD
(K)VEAKEES*EESDEDM#GFGLFD / 304 / 2
2 / 4.19 – 2
3.76 - 2
(K)VEAKEES*EES*DEDMGFGLFD
(K)VEAKEES*EES*DEDM#GFGLFD
(A)KEES*EES*DEDMGFGLFD / 304, 307 / 1
3
1 / 3.22 – 2
3.24 – 2
3.11 - 2
16 / Fibrinogen Alpha Chain / P02671 / (T)ADS*GEGDFLAEGGGVR / 22 / 3 / 4.24 - 2
17 / Osteopontin (Secreted Phosphoprotein 1) / P10451 / (N)ES*HDHMDDMDDEDDDDHVDS / 81 / 1 / 3.21 - 2
18 / 40S Ribosomal Protein S3 / P23396 / (K)DEILPTTPIS*EQK / 221 (224) / 2 / 2.75 - 2
19 / Histone H4 / P62805 (previously P02304) / (K)RIS*GLIYEETR / 47 / 2 / 1.97- 2
20 / Hsc70-Interacting Protein / P50502 / (K)KVEEDLKADEPSS*EES*DLEIDK / 75(76), 79 / 1 / 4.01 - 3
21 / Heterogeneous nuclear ribonucleoproteins C1/C2 / P07910 / (R)GDDQLELIKDDEKEAEEGEDDRDS*ANGEDDS / 299 / 1 / 5.49 - 3
22 / Septin 2 / Q15019 / (K)IYHLPDAES*DEDEDFKEQTR / 218 / 1 / 2.54 - 3
23 / RNA-binding protein Raly / Q9UKM9 / (R)TRDDGDEEGLLTHS*EEELEHSQDTDADDGALQ / 286 (288) / 1 / 5.11 - 3
(R)TRDDGDEEGLLTHS*EEELEHS*QDTDADDGALQ / 286 (288), 295 / 1 / 4.49 - 3
24 / Vitronectin / P04004 / (R)DS*WEDIFELLFWGR / 312 / 2 / 2.55 - 2
25 / Heat shock protein HSP 90-alpha / P07900 / (K)ESEDKPEIEDVGS*DEEEEKKDGDK / 262 / 1 / 3.53 - 3
26 / Adenylyl cyclase-associated protein 1 / Q01518 / (K)EMNDAAMFY*TNR / 163 / 2 / 2.74 - 2

a The phosphorylation site assigned based on the MS/MS spectrum of the particular peptide. If an exact site could not be assigned, the alternative site is shown in parenthesis.

b This column shows identification of the particular peptide in multiple IPG strips: 3 – the peptide was identified from all three strips; 2 – the peptide was detected in any two of the strips; 1- the peptide was identified from a single strip only.