Table S1 Clinical and Peri-Operative Factors Relative to Sarcopenia and Visceral Fat Amount

Table S1 Clinical and peri-operative factors relative to sarcopenia and visceral fat amount in HCC patients

Clinical factors / All patients
(N = 91) (%) / Sarcopenia / P / Visceral fat amount / P
Present
(n = 45) (%) / Absent
(n = 46) (%) / Low
(n = 47) (%) / High
(n = 44) (%)
Age (years) / 65.7 ± 9.1 / 65.2 ± 10 / 66.2 ± 7.6 / 0.59 / 64.9 ± 9.4 / 67 ± 8.7 / 0.4
Sex (male / female) / 76 / 15 / 38 / 7 / 38 /8 / 0.62 / 40 / 7 / 36 / 8 / 0.93
Skeletal muscle mass (cm²/m²) / 41.9 ± 6.8 / 37.1 ± 4.1 / 46.6 ± 5.6 / ＜0.001 / 40.4 ± 6.5 / 43.5 ± 6.9 / 0.029
Body mass index (kg/m²) / 23.2 ± 4.4 / 22.3 ± 5.5 / 24.0 ± 2.8 / 0.07 / 22.1 ± 5.1 / 24.3 ± 3 / 0.015
Visceral fat amount (cm²) / 92.9 ± 56.3 / 82.1 ± 54.5 / 103.2 ± 56.6 / 0.075 / 49.7 ± 29.9 / 138.1 ± 39.2 / ＜0.001
Albumin (g/dl) / 3.9 ± 0.5 / 3.8 ± 0.5 / 3.9 ± 0.5 / 0.84 / 3.8 ± 0.5 / 4.0 ± 0.5 / 0.07
Total bilirubin (mg/dl) / 0.85 ± 0.4 / 0.8 ± 0.4 / 0.9 ± 0.3 / 0.19 / 0.8 ± 0.3 / 0.9 ± 0.4 / 0.15
Platelet count (×104/μl) / 19.5 ± 28.6 / 18 ± 8.4 / 20.8 ± 39.1 / 0.66 / 16.9 ± 9.2 / 21.9 ± 38.8 / 0.42
ICGR15 (%) / 13.8 ± 8.2 / 13.8 ± 9.1 / 13.7 ± 7.4 / 0.94 / 13.2 ± 7.8 / 14.3 ± 8.6 / 0.54
Viral infection (HBV / HCV / nonBnonC) / 24 / 38 / 27 / 10 / 12 / 12 / 14 / 16 / 15 / 0.76 / 10 / 16 / 13 / 14 / 22 / 14 / 0.42
Preoperative therapy (Present / Absent) / 33 / 58 / 19 / 26 / 14 / 32 / 0.21 / 19 / 28 / 14 / 30 / 0.007
Tumor size (cm) / 7.1 ± 4.6 / 7.8 ± 5.1 / 6.5 ± 4 / 0.23 / 8.1 ± 5.4 / 6.2 ± 3.6 / 0.31
Number of tumors / 2.2 ± 1.8 / 2.3 ± 2.2 / 2.1 ± 1.3 / 0.69 / 2.4 ± 2.2 / 2 ±1.3 / 0.052
Transfusion / 5 (5.5%) / 4 (8.9%) / 1 (2.2%) / 0.06 / 3 (6.4%) / 2 (4.5%) / 0.57
Operative time (min) / 437 ± 98 / 432 ± 96 / 442 ± 101 / 0.67 / 421 ± 93 / 452 ± 101 / 0.15
Blood loss (ml) / 632 ± 1314 / 807 ± 1846 / 462 ± 261 / 0.13 / 782 ± 1840 / 486 ± 322 / 0.31
Operative methods
Left hepatectomy / 28 (31%) / 13 (29%) / 15 (33%) / 0.82 / 16 (34%) / 12 (27%) / 0.79
Left trisegmentectomy / 3 (3.3%) / 1 (2.2%) / 2 (4.3%) / 0.34 / 1 (2.1%) / 2 (4.5%) / 0.41
Right hepatectomy / 52 (57%) / 27 (60%) / 25 (54%) / 0.88 / 26 (55%) / 26 (59%) / 0.81
Right trisegmentectomy / 4 (4.4%) / 2 (4.4%) / 2 (4.3%) / 0.97 / 3 (6.4%) / 1 (2.3%) / 0.23
Central bisegmentectomy / 4 (4.4%) / 2 (4.4%) / 2 (4.3%) / 0.97 / 1 (2.1%) / 3 (6.8%) / 0.19

Table S2Short-term outcome in HCC patients with and without sarcopenia, the amount of visceral fat.

Postoperative complications / All patients / Patients aged >70 years
Sarcopenia / P / Visceral fat amount / P / Sarcopenia / P / Visceral
fat amount / P
Present
(n = 45) / Absent
(n = 46) / Low
(n= 47) / High
(n = 44) / Present
(n = 13) / Absent
(n = 17) / Low
(n = 12) / High
(n = 18)
Morbidity / 16 / 13 / 0.46 / 16 / 13 / 0.65 / 8 / 4 / 0.035 / 6 / 6 / 0.36
Liver-related morbidity / 7 / 1 / 0.024 / 4 / 4 / 0.92 / 5 / 1 / 0.0271 / 3 / 3 / 0.58
Mortality / 6 / 0 / 0.01 / 4 / 2 / 0.35 / 5 / 0 / 0.0051 / 2 / 3 / 1

Table S3 Univariable and multivariable analysis of clinical factors and liver-related morbidity and mortality in HCC patients

Clinical factors / Morbidity / Liver-related morbidity / Mortality
Univariable analysis / Multivariable analysis / Univariable analysis / Multivariable analysis / Univariable analysis / Multivariable analysis
Hazard ratio / P / Hazard ratio / P / Hazard ratio / P / Hazard ratio / P / Hazard ratio / P / Hazard ratio / P
Age≧65 years / 1.9 (0.77, 4.87) / 0.16 / 4.27 (0.15, 6.51) / 0.039 / 8.0 (2.83, 9.53) / 0.0046 / 2.31 (0.23, 4.4) / 0.13
Sex Male / 2.98 (0.42, 4.51) / 0.07 / 1.41 (0.34, 2.42) / 0.74 / 1.01 (0.05, 6.95) / 0.99
Sarcopenia / 1.4 (0.58, 3.43) / 0.46 / 5.64 (0.12, 8.29) / 0.0175 / 14.9 (0.059, 17) / 0.0001 / 8.88 (2.36, 42.3) / 0.0029 / 9.45 (4.5,10.2) / 0.0021
Body mass index
≦22.8 kg/m² / 1.07 (0.44, 2.61) / 0.88 / 1.02 (0.23, 4.59) / 0.97 / 2.05 (0.38, 15.3) / 0.41
Low visceral fat amount / 1.23 (0.51, 3.01) / 0.65 / 1.08 (0.24, 4.82) / 0.92 / 2.25 (0.42, 16.9) / 0.35
Albumin ≧4 g/dl / 0.25 (0.012, 1.24) / 0.03 / 0.75 (0.39, 1.12) / 0.43 / 0.66 (0.09, 3.6) / 0.64
Total bilirubin≧0.8 mg/dl / 0.88 (0.35, 2.19) / 0.78 / 0.96 (0.44, 2.26) / 0.33 / 0.57 (0.076, 3.08) / 0.52
Platelet count
≧15.3×104/μ / 1.15 (0.46, 2.9) / 0.76 / 0.21 (0.01, 0.98) / 0.036 / 0.39 (0.08, 1.21) / 0.073
ICGR15≧11.9% / 1.08 (0.43, 2.71) / 0.87 / 1.37 (0.38, 2.63) / 0.24 / 1.39 (0.65, 2.64) / 0.41
HCV infection / 1.02 (0.42, 2.54) / 0.96 / 3.99 (0.21, 4.78) / 0.046 / 6.9 (0.38, 2.62) / 0.0086 / 11.1 (1.93, 51.9) / 0.0009 / 5.66 (1.1, 9.1) / 0.0173
Preoperative therapy / 1.19 (0.48, 3.09) / 0.7 / 2.27 (0.32, 3.1) / 0.13 / 2.27 (0.27, 3.67) / 0.13
Tumor size≧6.5cm / 1.43 (0.57, 3.64) / 0.44 / 1.33 (0.28, 7.14) / 0.72 / 1.02 (0.18,5.84) / 0.98
Multiple tumors / 0.92 (0.36, 2.3) / 0.86 / 0.95 (0.44, 2.26) / 0.33 / 0.45 (0.012, 3.2) / 0.52
Transfusion / 1.39 (0.27, 3.98) / 0.59 / 1.12 (0.28, 3.6) / 0.34 / 1.29 (0.21,4.6) / 0.42
Operative time ≧417min / 2.64 (1.04, 7.1) / 0.04 / 10.2 (6.39, 15.6) / 0.0014 / 14.4 (0.059, 16.8) / 0.0001 / 8.6 (5.9,17.1) / 0.0033 / 11.7 (6.4, 15.6) / 0.0006
Blood loss≧400ml / 2.41 (0.94, 6.55) / 0.07 / 4.16 (0.15, 6.67) / 0.041 / 3.02 (0.19, 5.41) / 0.083

Values in parentheses are 95 per cent confidence intervals. ICGR15, indocyanine green dye retention test at 15 min

Table S4Univariable and multivariable analysis of clinical factors and morbidity, liver-related morbidity and mortality in HCC patients aged >70years.

Morbidity / Liver-related morbidity / Mortality
Clinical factors / Univariable analysis / Multivariable analysis / Univariable analysis / Multivariable analysis / Univariable analysis / Multivariable analysis
Hazard ratio / P / Hazard ratio / P / Hazard ratio / P / Hazard ratio / P / Hazard ratio / P / Hazard ratio / P
Sex Male / 7 (1.0, 142) / 0.0496 / 1.87 (0.23, 3.8) / 0.52 / 1.54 (0.31, 5.4) / 0.71
Sarcopenia / 5.2 (1.13, 28.1) / 0.034 / 5.2 (1.38, 12.5) / 0.023 / 9.9 (1.3, 209) / 0.024 / 8.5 (3.6, 44.5) / 0.0035 / 9.7 (1.14, 10.7) / 0.0018 / 6.73 (1.25, 8.0) / 0.0095
Body mass index
≦23.1kg/m² / 1.12 (0.25, 5.0) / 0.88 / 0.6 (0.09, 3.85) / 0.58 / 2.67 (0.38, 23.3) / 0.32
Low visceral fat amount / 0.7 (0.05, 4.3) / 0.36 / 0.72 (0.26, 10.8) / 0.58 / 1.0 (0.12,7.12) / 1.0
Albumin≧4 g/dl / 1.04 (0.22, 4.77) / 0.96 / 1.27 (0.18, 10.9) / 0.81 / 1.27 (0.018, 10.9) / 0.81
Total bilirubin≧0.8 mg/dl / 0.42 (0.08, 2.1) / 0.078 / 0.89 (0.35, 2.86) / 0.35 / 0.63 (0.35, 1.52) / 0.34
Platelet count
≧15.7×104/μl / 0.52 (0.07, 2.3) / 0.13 / 0.65 (0.25, 1.3) / 0.2 / 0.69 (0.25, 3.2) / 0.3
ICGR15≧12.4% / 0.57 (0.11, 2.6) / 0.47 / 1.62 (0.25, 4) / 0.2 / 1.62 (0.25, 4) / 0.2
HCV infection / 2.4 (0.51, 13.6) / 0.27 / 1.42 (0.28, 3.57) / 0.23 / 5.1 (0.67, 7.6) / 0.024 / 6.0 (1.37, 7.28) / 0.014
Preoperative therapy / 1.43 (0.31, 6.61) / 0.64 / 2.82 (0.21, 4.86) / 0.093 / 4.8 (1.26, 10.3) / 0.029
Tumor size≧6.5cm / 5.3 (1.1, 32.1) / 0.037 / 6.8 (2.0, 10.1) / 0.009 / 1.77 (0.25, 15.4) / 0.56 / 1.78 (0.25, 15.4) / 0.56
Multiple tumors / 1.04 (0.22, 4.77) / 0.96 / 1.27 (0.18, 10.9) / 0.81 / 1.27 (0.18, 10.9) / 0.81
Transfusion / 0.9 (0.094, 10.6) / 0.34 / 1.46 (0.26, 2.44) / 0.58 / 1.4 (0.31, 38.2) / 0.58
Operative time≧417min / 4.19 (0.88, 24.6) / 0.0727 / 7.6 (5.4, 18.7) / 0.0059 / 6.7 (2.9, 34.6) / 0.0094 / 7.57 (5.4, 18.7) / 0.0059 / 7.23 (1.06, 9.44) / 0.0071
Blood loss≧400ml / 4.89 (1.0, 29.6) / 0.0497 / 4.2 (1.12, 10.7) / 0.039 / 2.32 (0.19, 5.2) / 0.13 / 2.32 (0.19, 5.2) / 0.13

Values in parentheses are 95 per cent confidence intervals. ICGR15, indocyanine green dye retention test at 15 min