Table S1 Locality data for all specimens included in phylogenetic and genetic distance analyses. The straight line distance from the focal population to each specimen locality is also given.

Dataset / Individual Code / Species / Locality / Distance to Focal Population (km) / Reference
AFLP / BAR001 / E. barrenense / East Fork Barren River, Monroe Co., KY / 0 / a
BAR002 / E. barrenense / East Fork Barren River, Monroe Co., KY / 0 / a
BAR105 / E. barrenense / East Fork Barren River, Monroe Co., KY / 0 / a
BAR106 / E. barrenense / East Fork Barren River, Monroe Co., KY / 0 / a
CRV6 / E. cervus / Clarks Creek, Chester Co., TN / 0 / a
CRV7 / E. cervus / Clarks Creek, Chester Co, TN / 0 / a
CSE002 / E. coosae / Conasauga River, Polk Co., TN / 0 / a
CSE003 / E. coosae / Conasauga River, Polk Co., TN / 0 / a
CSE016 / E. coosae / Conasauga River, Polk Co., TN / 0 / a
CSE018 / E. coosae / Conasauga River, Polk Co., TN / 0 / a
DUR001 / E. duryi / Sweeten Creek, Marion Co., TN / 0 / a
DUR002 / E. duryi / Sweeten Creek, Marion Co., TN / 0 / a
DUR005 / E. duryi / Sweeten Creek, Marion Co., TN / 0 / a
DUR007 / E. duryi / Sweeten Creek, Marion Co., TN / 0 / a
INS201 / E. inscriptum / Little Eastatoe Creek, Pickens Co., SC / 0 / a
INS202 / E. inscriptum / Little Eastatoe Creek, Pickens Co., SC / 0 / a
INS203 / E. inscriptum / Little Eastatoe Creek, Pickens Co., SC / 0 / a
INS204 / E. inscriptum / Little Eastatoe Creek, Pickens Co., SC / 0 / a
SIM012 / E. orientale / Fishing Creek, Pulaski Co., KY / 0 / a
SIM014 / E. orientale / Fishing Creek, Pulaski Co., KY / 0 / a
SIM021 / E. orientale / Fishing Creek, Pulaski Co., KY / 0 / a
SIM026 / E. orientale / Fishing Creek, Pulaski Co., KY / 0 / a
TN1615 / E. pyrrhogaster / Terrapin Creek, Graves Co., KY / 25 / a
P2 / E. pyrrhogaster / Clear Creek, Henry Co., TN / 22 / a
PYR800 / E. pyrrhogaster / Terrapin Creek, Graves Co., KY / 25 / a
PYR801 / E. pyrrhogaster / Terrapin Creek, Graves Co., KY / 25 / a
PYR802 / E. pyrrhogaster / Terrapin Creek, Graves Co., KY / 25 / a
TN1606 / E. ramseyi / Beaver Creek, Wilcox Co., AL / 129 / a
RM1 / E. ramseyi / Little Creek, Merengo Co., AL / 122 / a
UCR1 / E. ramseyi / Cahaba River, Jefferson Co., AL / 68 / a
TN1610 / E. raneyi / Big Spring Creek, Marshall Co., MS / 0 / a
Y2 / E. raneyi / Big Spring Creek, Marshall Co., MS / 0 / a
TN5614 / E. tallapoosae / Cane Creek, Cleburne Co., AL / 58 / a
T1 / E. tallapoosae / Verdin Creek, Cleburne Co., AL / 46 / a
T2 / E. tallapoosae / Enitachopco Creek., Clay Co., AL / 8 / a
T3 / E. tallapoosae / Jumpin In Creek, Carroll Co., GA / 62 / a
THA001 / E. thalassinum / Middle Saluda River, Greenville Co., SC / 0 / a
THA201 / E. thalassinum / Middle Saluda River, Greenville Co., SC / 0 / a
THA202 / E. thalassinum / Middle Saluda River, Greenville Co., SC / 0 / a
THA204 / E. thalassinum / Middle Saluda River, Greenville Co., SC / 0 / a
THA205 / E. thalassinum / Middle Saluda River, Greenville Co., SC / 0 / a
TN1562 / E. zonistium / Lick Creek, McNairy Co., TN / 0 / a
Zi2 / E. zonistium / Sycamore Creek, Benton Co., TN / 92 / a
Zi3 / E. zonistium / West Sandy Creek, Henry Co., TN / 133 / a
ZNI813 / E. zonistium / Lick Creek, McNairy Co., TN / 0 / a
ZNI814 / E. zonistium / Lick Creek, McNairy Co., TN / 0 / a
ZNI815 / E. zonistium / Lick Creek, McNairy Co., TN / 0 / a
DNA Sequence / EbrnA / E. barrenense / Long Creek, Macon Co., TN / 29 / b
EbrnB / E. barrenense / Trammel Fork, Allen Co., KY / 55 / b
EcerA / E. cervus / Clarks Creek, Chester Co., TN / 0 / b
EcerB / E. cervus / Clarks Creek, Chester Co., TN / 0 / b
EcooB / E. coosae / Conasauga River, Polk Co., TN / 3 / b
EcooC / E. coosae / Trib. to Coosa River, Etowah Co., AL / 162 / b
EdurA / E. duryi / Mulepen Creek, Lincoln Co., TN / 59 / b
EdurB / E. duryi / Sequatchie River, Cumberland Co., TN / 104 / b
EinsA / E. inscriptum / Chandler Creek, Hall Co., GA / 105 / b
EoriA / E. orientale / Fishing Creek, Lincoln/Casey Co., KY / 11 / b
EoriC / E. orientale / West Fork Obey River, Overton Co., TN / 106 / b
EpyrA / E. pyrrhogaster / Terrapin Creek, Weakley Co., TN / 23 / b
EpyrB / E. pyrrhogaster / Terrapin Creek, Graves Co., KY / 25 / b
EramA / E. ramseyi / Beaver Creek, Wilcox Co., AL / 129 / b
EramB / E. ramseyi / Beaver Creek, Wilcox Co., AL / 129 / b
EranA / E. raneyi / Big Spring Creek, Marshall Co., MS / 2 / b
EranB / E. raneyi / Pumpkin Creek, Lafayette Co., MS / 43 / b
EtalA / E. tallapoosae / Cane Creek, Cleburne Co., AL / 58 / b
EtalB / E. tallapoosae / Muscadine Creek, Cleburne Co., AL / 65 / b
EthaA / E. thalassinum / Middle Little River, Caldwell Co., NC / 137 / b
EthaB / E. thalassinum / Green River, Polk Co., NC / 30 / b
EzniA / E. zonistium / Panther Creek, Henry Co., TN / 122 / b
EzniB / E. zonistium / Lick Creek, McNairy Co., TN / 0 / b

a - Mendelson and Wong 2010

b - Near et al. 2011