Supplementary Table 1.Plasmids and NG MS11-A derived reporter strains used in this study.

Reporter plasmids / Description / Reporter strains / Reference
pJKD699 / Contains a promoterlesscat (chloramphenicol resistance) gene (from pCM4) in BamHI pGEM-3Z / (1)
pJKD3172 / pJKD1854 (1) with SmaI Ω cassette (spectinomycin resistance) from pHP45Ω inserted in the unique NsiI site found in iga sequence of pJKD1854 in same orientation as iga sequence. Vector used for the construction of pil promoter cat (Ppil::cat) reporter constructs / JKD5222 / C.S. Ryan, unpublished
pJKD3181 / WT pilF promoter (PpilF) region without a CREE amplified from NG strain FA1090
P amplified with DAP1576/DAP1575
cat amplified with DAP1574/M13RP
PpilF::cat fusion PCR with DAP1576/M13RP / JKD5245 / This study
JKD3271 / WT pilHpromoter (PpilH) contains a CREE (+IHF BS) amplified from NG strain FA1090
Promoter (P) amplified with DAP2053/DAP1569
cat amplified with DAP1568/M13RP
PpilH::cat fusion PCR with DAP2053/M13RP / JKD5244 / This study
pJKD3274 / WT pilG promoter (PpilG) region with a CREE (+IHF BS) amplified from NG strain FA1090
P amplified with DAP1573/DAP1572
cat amplified with DAP1571/M13RP
PpilG::cat fusion PCR with DAP1573/M13RP / JKD5243 / This study
pJKD3285 / Derivative of pJKD3274 with the 5’ CR and the sequence upstream of it deleted
Portion of PpilG::cat amplified with DAP1752/M13RP / JKD5253 / This study
pJKD3286 / Derivative of pJKD3274 with the entire CREE deleted
Portion of PpilG::cat amplified with DAP1753/M13RP / JKD5259 / This study
pJKD3289 / Derivative of pJKD3271 with sequence immediately upstream of CREE deleted
Portion of PpilH::cat amplified with DAP1751/M13RP / JKD5250 / This study
pJKD3298 / Derivative of pJKD3271 contains only the sequence immediately upstream of CREE
P amplified with DAP2053/DAP1763
cat amplified with DAP1762/M13RP
PpilH::cat fusion PCR with DAP2053/M13RP / JKD5256 / This study
pJKD3306 / Derivative of pJKD3271 with P1 mutated to CCCCCC
P amplified with DAP2053/DAP1936
cat amplified with DAP1935/M13RP
PpilH::cat fusion PCR with DAP2053/M13RP / JKD5261 / This study
pJKD3307 / Derivative of pJKD3181 with P1 mutated to CCCCCC
P amplified with DAP1576/DAP1938
cat amplified with DAP1937/M13RP
PpilF::cat fusion PCR with DAP1576/M13RP / JKD5262 / This study
pJKD3320 / Derivative of pJKD3181 with P2 mutated to GGGGGG
P amplified with DAP1576/DAP1948
cat amplified with DAP1947/M13RP
PpilF::cat fusion PCR with DAP1576/M13RP / JKD5265 / This study
pJKD3322 / Derivative of pJKD3271 with P2 mutated to GGGGGG
P amplified with DAP2053/DAP1950
cat amplified with DAP1949/M13RP
PpilH::cat fusion PCR with DAP2053/M13RP / JKD5264 / This study
pJKD3336 / WT PpilF contains a CREE (+IHF BS) amplified from NM strain FAM18
P amplified with DAP1576/DAP1575
cat amplified with DAP1574/M13RP
PpilF::cat fusion PCR with DAP1576/M13RP / JKD5271 / This study
pJKD3338 / WT PpilH promoter without a CREE amplified from NG strain MS11-A
P amplified with DAP2053/DAP2055
cat amplified with DAP2054/M13RP
PpilH::cat fusion PCR with DAP2053/M13RP / JKD5269 / This study
pJKD3355 / Derivative of pJKD3274 with P1 mutated to ATCTTG
P amplified with DAP1573/DAP2057
cat amplified with DAP2056/M13RP
PpilG::cat fusion PCR with DAP1573/M13RP / JKD5275 / This study

REFERENCE

1.Fyfe, J. A. M., R. A. Strugell, and J. K. Davies. 1993. Control of gonococcal pilin-encoding gene expression in Escherichia coli. Gene 123:45-50.