Reliability of the nanopheres-DNA immunization technology to produce polyclonal antibodies directed against human neogenic proteins
Ahmed Arnaoty, Valérie Gouilleux, Sophie Casteret, Bruno Pitard,
Yves Bigot and Thierry Lecomte
Supplementary Material 1 : Protein sequence by each of the 24 neogenes. Regions in protein that have being retained for vaccination are typed in red.
ZBED1 (NP_004720)
1 menkslessq tdlklvahpr akskvwkyfg fdtnaegcil qwkkiycric maqiaysgnt
61 snlsyhlekn hpeefcefvk snteqmreaf atafsklkpe ssqqpgqdal avkaghgyds
121 kkqqeltaav lgliceglyp asivdeptfk vllktadpry elpsrkyist kaipekygav
181 revilkelae atwcgistdm wrsenqnray vtlaahflgl gapnclsmgs rclktfevpe
241 entaetitrv lyevfiewgi sakvfgattn ygkdivkacs lldvavhmpc lghtfnagiq
301 qafqlpklga llsrcrklve yfqqsavamy mlyekqkqqn vahcmlvsnr vswwgstlam
361 lqrlkeqqfv iagvlvedsn nhhlmlease watieglvel lqpfkqvaem lsasryptis
421 mvkpllhmll nttlniketd skelsmakev iakelsktyq etpeidmfln vatfldpryk
481 rlpflsafer qqvenrvvee akglldkvkd ggyrpaedki fpvpeeppvk klmrtstppp
541 asvinnmlae ifcqtggved qeewhaqvve elsnfksqkv lglnedplkw wsdrlalfpl
601 lpkvlqkywc vtatrvaper lfgsaanvvs akrnrlapah vdeqvflyen arsgaeaepe
661 dqdegewgld qeqvfslgdg vsggffgird ssfl
ZBED5 (NP_001137139.1)
1 miapllcils ynfntfailn vyskltmfct tnslpmdlll kqgslkqeve sfcyqivses
61 ndqkvgilqs edkqlqpsvs kksegelsrv kfisnsnkit fskkpkrrky desylsfgft
121 yfgnrdapha qcvlckkils nsslapsklr rhletkhaay kdkdisffkq hldspennkp
181 ptpkivntdn esateasynv syhialsgea htigellikp cakdvvmrmf deqyskkida
241 vqlsnstvar rikdlaadie eelvcrlkic dgfslqldes advsglavll vfvryrfnks
301 ieedlllces lqsnatgeei fncinsfmqk heiewekcvd vcsdasravd gkiaeavtli
361 kyvapestss hcllyrhala vkimptslkn vldqavqiin yikarphqsr llkilceemg
421 aqhtalllnt evrwlsrgkv lvrlfelrre llvfmdsafr lsdcltnssw llrlayladi
481 ftklnevnls mqgknvtvft vfdkmssllr klefwassve eenfdcfptl sdflteinst
541 vdkdicsaiv qhlrglratl lkyfpvtndn nawvrnpftv tvkpaslvar dyeslidlts
601 dsqvkqnfse lslndfwssl iqeypsiarr avrvllpfat mhlcetgfsy yaatktkyrk
661 rldaaphmri rlsnitpnik ricdkktqkh csh
ZBED6 (NP_001167579.1)
1 msvctlsvpv sslspgrrcn tfsdsgilgc vpinsntdee dvveekmvae gvnkeakqpa
61 kkkrkkglri kgkrrrkkli lakkfskdlg sgrpvadapa llasndpeqd eeslfesnie
121 kqiylpstra ktsivwhffh vdpqytwrai cnlceksvsr gkpgshlgts tlqrhlqarh
181 sphwtrankf gvasgeedft ldvslspssg sngsfeyipt dplddnrmgk khdksasdal
241 raergrflik snivkhalip gtraktsavw nffytdpqhi sravcnickr svsrgrpgsh
301 lgtstlqrhl qathpihwav ankdsgavan gldeaeters dllsdtlhge kstgsqdlta
361 edlsdsdsde pmlevenrse spipvaeqgt lmraqerett ccgnpvsshi sqaiiqmive
421 dmhpynyfst pafqrfmqiv apdyrlpset yfftkavpql ydcvrekifl tlenvqsqki
481 hltvdiwthd pstdyfivtv hwvsletasf lnngripdfr kwavlcvtgl akdclitnil
541 qelndqiglw lspnflipsf ivsdnssnvv haikdggfth vpcflhclnm viqdffcehk
601 sienmlvaar ktchhfshsv karqilqefq ndhqlpwknl kqdetghwis tfymlkwlle
661 hcysvhhslg rasgvvltsl qwtlmtyvcd ilkpfeeatq kvsvktagln qvlplihhll
721 lslqklredf qvrgitqaln lvdslslkle tdtllsamlk skpcilatll dpcfknsled
781 ffpqgadlet ykqflaeevc nymesspeic qiptseascp svtvgadsft sslkegtsss
841 gsvdssavdn valgsksfmf psavavvdey fkekysefsg gddpliywqr kisiwpaltq
901 vaiqylscpm cswqsecift knshfhpkqi msldfdnieq lmflkmnlkn vnydystlvl
961 swdpeqnevv qssekeilp
Charlie8 (NP_001003795.1)
1 MAQVAVSTLP VEEESSSETR MVVTFLVSAL ESMCKELAKS KAEVACIAVY ETDVFVVGTE
61 RGCAFVNART DFQKDFAKYC VAEGLCEVKP PCPVNGMQVH SGETEILRKA VEDYFCFCYG
121 KALGTTVMVP VPYEKMLRDQ SAVVVQGLPE GVAFQHPENY DLATLKWILE NKAGISFIIN
181 RPFLGPESQL GGPGMVTDAE RSIVSPSESC GPINVKTEPM EDSGISLKAE AVSVKKESED
241 PNYYQYNMQG SHPSSTSNEV IEMELPMEDS TPLVPSEEPN EDPEAEVKIE GNTNSSSVTN
301 SAAGVEDLNI VQVTVPDNEK ERLSSIEKIK QLREQVNDLF SRKFGEAIGV DFPVKVPYRK
361 ITFNPGCVVI DGMPPGVVFK APGYLEISSM RRILEAAEFI KFTVIRPLPG LELSNVGKRK
421 IDQEGRVFQE KWERAYFFVE VQNIPTCLIC KQSMSVSKEY NLRRHYQTNH SKHYDQYTER
481 MRDEKLHELK KGLRKYLLGS SDTECPEQKQ VFANPSPTQK SPVQPVEDLA GNLWEKLREK
541 IRSFVAYSIA IDEITDINNT TQLAIFIRGV DENFDVSEEL LDTVPMTGTK SGNEIFLRVE
601 KSLKKFCINW SRLVSVASTG TPAMVDANNG LVTKLKSRVA TFCKGAELKS ICCIIHPESL
661 CAQKLKMDHV MDVVVKSVNW ICSRGLNHSE FTTLLYELDS QYGSLLYYTE IKWLSRGLVL
721 KRFFESLEEI DSFMSSRGKP LPQLSSIDWI RDLAFLVDMT MHLNALNISL QGHSQIVTQM
781 YDLIRAFLAK LCLWETHLTR NNLAHFPTLK LVSRNESDGL NYIPKIAELK TEFQKRLSDF
841 KLYESELTLF SSPFSTKIDS VHEELQMEVI DLQCNTVLKT KYDKVGIPEF YKYLWGSYPK
901 YKHHCAKILS MFGSTYICEQ LFSIMKLSKT KYCSQLKDSQ WDSVLHIAT
JRK (NP_003715.2)
1 maskpaagks rgekrkrvvl tlkekidict rlekgesrka lmqeynvgms tlydirahka
61 qllrffassd snkaleqrrt lhtpklehld rvlyewflgk rsegvpvsgp mliekakdfy
121 eqmqltepcv fsggwlwrfk arhgikklda ssekqsadhq aaeqfcaffr slaaehglsa
181 eqvynadetg lfwrclpnpt peggavpgpk qgkdrltvlm canatgshrl kplaigkcsg
241 prafkgiqhl pvaykaqgna wvdkeifsdw fhhifvpsvr ehfrtiglpe dskavlllds
301 srahpqeael vssnvftifl pasvaslvqp meqgirrdfm rnfinppvpl qgpharynmn
361 daifsvacaw navpshvfrr awrklwpsva faegssseee leaecfpvkp hnksfahile
421 lvkegsscpg qlrqrqaasw gvagreaegg rppaatspae vvwssektpk adqdgggdpg
481 egeevaweqa avafdavlrf aerqpcfsaq evgqlralra vfrsqqqvrr rrgalgavvk
541 vealqegpgg cgataqsplp csstagdn
TIGD2 (NP_663761)
1 mlgkrkrvvl tikdkldiik kleegisfkk lsvvygiges tvrdikknke riinyanssd
61 ptsgvskrks mksstyeeld rvmiewfnqq ktdgipvsgt icakqakfff dalgmegdfn
121 assgwltrfk qrhgipkaag kgtklkgdet aarefcgsfq efvekenlqp eqiygadqtg
181 lfwkclpsrt ltletdqsts gcrssrerii imccanatgl hklnlcvvgk akkprafkgt
241 dlsnlpvtyy sqkgawieqs vfrqwfekyf vpqvqkhlks kgllekavll ldfpparpne
301 emlssddgri ivkylppnvt sliqpmsqgv latvkryyra gllqkymdeg ndpkifwknl
361 tvldaiyevs rawnmvksst itkawkklfp gneensgmni degailaanl atvlqnteec
421 ehvdienidq wfdsrssdss cqvltdsesa edqtkaaeqk pssksrktel npekhishka
481 alewtenlld yleqqddmll sdklvlrrlr tiirkkqkiq nnknh
TIGD3 (NP_663771.1)
1 melsskkklh alslaekiqv lelldeskms qsevarrfqv sqpqisrick nkeklladwc
61 sgtanrerkr kreskysgid eallcwyhia rakawdvtgp mllhkakela dimgqdfvps
121 igwlvrwkrr nnvgfgarhv lapsfppepp ppgltsqaql plslkdfspe dvfgcaelpl
181 lyravpgsfg acdqvqvllc ansrgtekrr vllgglqaap rcffgirsea lpasyhpdlg
241 ipwlewlaqf drdmgqqgrq valllaarvv eelaglpgly hvkllplaas sttpplpssv
301 vrafkahyrh rllgklaaiq serdgtslae agagitvlda lhvasaawak vppqlifssf
361 iqeglapgkt ppsshktsem ppvpgglsle efsrfvdleg eeprsgvcke eigtedekgd
421 regafeplpt kadalralgt lrrwfecnst spelfekfyd ceeeverlcc
TIGD4 (NP_663772)
1 maeasvdast lpvtvkkkks lsieekidii navesgkkka eiaaeygikk nslssimknk
61 dkvleafesl rfdpkrkrlr tafytdleea lmrwyriaqc lnvpvngpml rlkandfaqk
121 lghndfkcsn gwldrfksry glvfraqpve atgvpvdpst vwyqnvlpyy lndyhpknvf
181 niketgllyr mlptntfafk getcsvgklc kdritlvvgt nmdgseklpl lvigkkrtph
241 cfkglkslpv cyeanrmawm tsdvfeqwmr kldeefqaqq rrvvifvesf pahpevknlk
301 sielaffpsc lsskciamkq gvikslkiky rhclikkfls svegskeftf slldavdtlh
361 lcwravtpet ivksyeeagf ksqkgesdit naekdtgldl vadalgagve fpeglsieey
421 aaldddletc eaapngdsic tkesksdetg fytsdeeddd gspgtelplp skseaitald
481 tlkkflrsqd mndglqnsla dlenfinsls pk
TIGD5 (NP_116251.3)
1 mafrkaysik dklqaiervk ggerqasvcr dfgvpggtlr gwlkdepklr wfleqlggev
61 gtqrkkmrla neeeidravy awflalrqhg vplsgpliqa qaeafarqiy gpectfkash
121gwfwrwqkrh gissqrfyge agppapspap gppvkeepal psgagplpdr apappppaeg
181 gygdeqiysa svtglywkll peqaappgag dpgaggcgrr wrgdrvtvll aanltgshkl
241 kplvigrlpd ppslrhhnqd kfpasyrysp dawlsrpllr gwffeefvpg vkrylrrscl
301 qqkavllvah ppcpspaasm paldsedapv rcrpeplgpp eelqtpdgav rvlflskgss
361 rahipapleq gvvaafkqly krellrlavs casgspldfm rsfmlkdmly laglswdlvq
421 agsiercwll glraafeprp gedsagqpaq aeeaaehsrv lsdlthlaal aykclapeev
481 aewlhldddg gppegcreev gpalppaapp apaslpsamg ggedeeeatd yggtsvptag
541 eavrgletal rwlenqdpre vgplrlvqlr slismarrlg gightpagpy dgv
TIGD6 (NP_112215.1)
1 mankgnkkrr qfsleekmkv vgavdsgkrk gdvakefgit pstlstflkd rtkfeekvre
61 asvgpqrkrm rsalyddidk avfawfqeih aknilvtgsv irkkalnlan mlgydnfqas
121 vgwlnrfrdr hgialkavcr edsdrlmngl gidkinewha geiikliady spddifnade
181 tgvffqllpq htlaakgdhc rggkkakqrl talfccnasg tekmrplivg rsasphclkn
241 ihslpcdyra nqwawmtrdl fnewlmqvda rmkraerril llidncsahn mlphleriqv
301 gylpsnctav lqplnlgiih tmkvlyqshl lkqillklns sedqeevdik qaidmiaaaw
361 wsvkpstvvk cwqkagivpm efaecdtesa asepdiaiek lwhtvaiatc vpnevnfqdf
421 vtadddliis qdtdiiqdmv agentseags edegevslpe qpkvtiteai ssvqklrqfl
481 stcvdipdai fgqlngidey lmkrvtqtli dskitdflqt k
TIGD7 (NP_149985)
1 mnkrgkyttl nleekmkvls rieagrslks vmdefgisks tfydikknkk lildfvlkqd
61 mplvgaekrk rttgakygdv ddavymwyqq krsagvpvrg velqaaaerf arcfgrtdfk
121 astgwlfrfr nrhaignrkg cgeqvlssvs envepfrqkl smiikeeklc laqlysgdet
181 dlfwksmpen sqasrkdicl pgkkinkerl saflcanadg thklksiiig ksklpksvke
241 dtstlpviyk pskdvwftre lfsewffqnf vpevrhfqln vlrfhdedvr alllldscpa
301 hpssesltse dgrikcmffp hntstliqpm nqgvilsckr lyrwkqlees lvifeesdde
361 qekgdkgvsk ikiyniksai fnwaksweev kqitianawe nllykkepey dfqglehgdy
421 reilekcgel etkldddrvw lngdeekgcl lktkggitke vvqkggeaek qtaefklsav
481 resldylldf vdatpefqrf hftlkemqqe ivkkqfqski hsrigsflkp rphnikdsfs
541 gpstsgsnh
POGK (NP_060012.3)
1 mestayplnl slkeeeeeee iqsreledgp admqkvrics eggwvpalfd evaiyfsdee
61 wevlteqqka lyrevmrmny etvlslefpf pkpdmitrle geeesqnsde wqlqggtsae
121 neesdvkppd wpnpmnatsq fpqpqhfdsf glrlprdite lpewsegypf ymamgfpgyd
181 lsaddiagkf qfsrgmrrsy dagfklmvve yaestnncqa akqfgvlekn vrdwrkvkpq
241 lqnahamrra frgpkngrfa lvdqrvaeyv rymqakgdpi treamqlkal eiaqemnipe
301 kgfkaslgwc rrmmrrydls lrhkvpvpqh lpedlteklv tyqrsvlalr rahdyevaqm
361 gnadetpicl evpsrvtvdn qgekpvlvkt pgreklkita mlgvladgrk lppyiilrgt
421 yippgkfpsg meirchrygw mtedlmqdwl evvwrrrtga vpkqrgmlil ngfrghatds
481 vknsmesmnt dmviipgglt sqlqvldvvv ykplndsvra qysnwllagn lalsptgnak
541 kpplglflew vmvawnsiss esivqgfkkc hissnleeed dvlweiesel pgggeppkdc
601 dtesmaesn
POGZ (NP_055915.2)
1 madtdlfmec eeeelepwqk isdviedsvv edynsvdktt tvsvsqqpvs apvpiaahas
61 vaghlststt vsssgaqnsd stkktlvtli annnagnplv qqggqplilt qnpapglgtm
121 vtqpvlrpvq vmqnanhvts spvasqpifi ttqgfpvrnv rpvqnamnqv givlnvqqgq
181 tvrpitlvpa pgtqfvkptv gvpqvfsqmt pvrpgstmpv rpttntfttv ipatltirst
241 vpqsqsqqtk stpststtpt atqptslgql avqspgqsnq ttnpklapsf psppavsias
301 fvtvkrpgvt gensnevakl vntlntipsl gqspgpvvvs nnssahgsqr tsgpessmkv
361 tssipvfdlq dggrkicprc naqfrvteal rghmcyccpe mveyqkkgks ldsepsvpsa
421 akppspekta pvastpsstp ipalspptkv pepnenvgda vqtklimlvd dfyygrdggk
481 vaqltnfpkv atsfrcphct krlknnirfm nhmkhhveld qqngevdght icqhcyrqfs
541 tpfqlqchle nvhspyestt kckicewafe seplflqhmk dthkpgempy vcqvcqyrss
601 lysevdvhfr mihedtrhll cpyclkvfkn gnafqqhymr hqkrnvyhcn kcrlqflfak
661 dkiehklqhh ktfrkpkqle glkpgtkvti rasrgqprtv pvssndtpps alqeaaplts
721 smdplpvfly ppvqrsiqkr avrkmsvmgr qtclecsfei pdfpnhfpty vhcslcryst
781 ccsrayanhm innhvprksp kylalfknsv sgiklactsc tfvtsvgdam akhlvfnpsh
841 rsssilprgl twiahsrhgq trdrvhdrnv knmypppsfp tnkaatvksa gatpaepeel
901 ltplapalps pastatpppt pthpqalalp plategaecl nvddqdegsp vtqepelasg
961 gggsggvgkk eqlsvkklrv vlfalccnte qaaehfrnpq rrirrwlrrf qasqgenleg
1021 kylsfeaeek laewvltqre qqlpvneetl fqkatkigrs leggfkisye wavrfmlrhh
1081 ltpharrava htlpkdvaen aglfidfvqr qihnqdlpls mivaideisl fldtevlssd
1141 drkenalqtv gtgepwcdvv lailadgtvl ptlvfyrgqm dqpanmpdsi lleakesgys
1201 ddeimelwst rvwqkhtacq rskgmlvmdc hrthlseevl amlsasstlp avvpagcssk
1261 iqpldvcikr tvknflhkkw keqaremadt acdsdvllql vlvwlgevlg vigdcpelvq
1321 rsflvasvlp gpdgninspt rnadmqeeli asleeqlkls gehsesstpr prsspeetie
1381 peslhqlfeg esetesfygf eeadldlmei
SETMAR (NP_006506.3) for sera directed against the SET domain
1 mfaeaakttr pcgmaefkek peapteqldv acgqenlpvg awppgaapap fqytpdhvvg
61 pgadidptqi tfpgcicvkt pclpgtcscl rhgenyddns clrdigsggk yaepvfecnv
121 lcrcsdhcrn rvvqkglqfh fqvfkthkkg wglrtlefip kgrfvceyag evlgfsevqr
181 rihlqtksds nyiiairehv yngqvmetfv dptyignigr flnhscepnl lmipvridsm
241 vpklalfaak divpeeelsy dysgrylnlt vsedkerldh gklrkpcycg aksctaflpf
301 dsslycpvek sniscgneke psmcgsapsv fpsckrltletmkmmldkkq iraiflfefk
361 mgrkaaettr ninnafgpgt anertvqwwf kkfckgdesl edeersgrps evdndqlrai
421 ieadpltttr evaeelnvnh stvvrhlkqi gkvkkldkwv pheltenqkn rrfevsssli
481 lrnhnepfld rivtcdekwi lydnrrrsaq wldqeeapkh fpkpilhpkk vmvtiwwsaa
541 glihysflnp getitsekya qeidemnqkl qrlqlalvnr kgpillhdna rphvaqptlq
601 klnelgyevl phppyspdll ptnyhvfkhl nnflqgkrfh nqqdaenafq efvesqstdf
661 yatginqlis rwqkcvdcng syfd
SETMAR (NP_006506.3) for sera directed against the Hsmar1 domain
1 mfaeaakttr pcgmaefkek peapteqldv acgqenlpvg awppgaapap fqytpdhvvg
61 pgadidptqi tfpgcicvkt pclpgtcscl rhgenyddns clrdigsggk yaepvfecnv
121 lcrcsdhcrn rvvqkglqfh fqvfkthkkg wglrtlefip kgrfvceyag evlgfsevqr
181 rihlqtksds nyiiairehv yngqvmetfv dptyignigr flnhscepnl lmipvridsm
241 vpklalfaak divpeeelsy dysgrylnlt vsedkerldh gklrkpcycg aksctaflpf
301 dsslycpvek sniscgneke psmcgsapsv fpsckrltle tmkmmldkkq iraiflfefk
361 mgrkaaettr ninnafgpgt anertvqwwf kkfckgdesl edeersgrps evdndqlrai
421 ieadpltttr evaeelnvnh stvvrhlkqi gkvkkldkwv pheltenqkn rrfevsssli
481 lrnhnepfld rivtcdekwi lydnrrrsaq wldqeeapkh fpkpilhpkk vmvtiwwsaa
541 glihysflnp getitsekya qeidemnqkl qrlqlalvnr kgpillhdna rphvaqptlq
601 klnelgyevl phppyspdll ptnyhvfkhl nnflqgkrfh nqqdaenafq efvesqstdf
661 yatginqlis rwqkcvdcng syfd
THAP4 (NP_057047.3)
1 mviccaavnc snrqgkgekr avsfhrfplk dskrliqwlk avqrdnwtpt kysflcsehf
61 tkdsfskrle dqhrllkpta vpsifhltek krgagghgrt rrkdaskatg gvrghssaat
121 grgaagwsps ssgnpmakpe srrlkqaalq geatpraaqe aasqeqaqqa lertpgdgla
181 tmvagsqgka easatdagde satssieggv tdksgismdd ftppgsgack figslhsysf
241 sskhtrerps vprepidrkr lkkdvepscs gsslgpdkgl aqsppssslt atpqkpsqsp
301 sappadvtpk pateavqseh sdaspmsine vilsasgack lidslhsycf ssrqnksqvc
361 clreqvekkn gelkslrqrv srsdsqvrkl qekldelrrv svpypsslls psreppkmnp
421 vveplswmlg twlsdppgag typtlqpfqy leevhishvg qpmlnfsfns fhpdtrkpmh
481 recgfirlkp dtnkvafvsa qntgvvevee gevngqelci ashsiarisf akephveqit
541 rkfrlnsegk leqtvsmatt tqpmtqhlhv tykkvtp
THAP9 (NP_078948.3)
1 mtrscsavgc strdtvlsre rglsfhqfpt dtiqrskwir avnrvdprsk kiwipgpgai
61 lcskhfqesd fesygirrkl kkgavpsvsl ykipqgvhlk gkarqkilkq plpdnsqeva
121 tedhnyslkt pltigaekla evqqmlqvsk krlisvknyr mikkrkglrl idalveekll
181 seetecllra qfsdfkwely nwretdeysa emkqfactly lcsskvydyv rkilklphss
241 ilrtwlskcq pspgfnsnif sflqrrveng dqlyqycsll iksmplkqql qwdpsshslq
301 gfmdfglgkl dadetplase tvllmavgif ghwrtplgyf fvnrasgylq aqllrltigk
361 lsdigitvla vtsdatahsv qmakalgihi dgddmkctfq hpssssqqia yffdschllr
421 lirnafqnfq siqfingiah wqhlvelval eeqelsnmer ipstlanlkn hvlkvnsatq
481 lfsesvasal eyllsldlpp fqncigtihf lrlinnlfdi fnsrncygkg lkgpllpety
541 skinhvliea ktifvtlsdt snnqiikgkq klgflgflln aeslkwlyqn yvfpkvmpfp
601 ylltykfshd hlelflkmlr qvlvtssspt cmafqkayyn letrykfqde vflskvsifd
661 isiarrkdla lwtvqrqygv svtktvfhee gicqdwshcs lsealldlsd hrrnlicyag
721 yvanklsall tcedcitaly asdlkaskig sllfvkkkng lhfpseslcr vinicervvr
781 thsrmaifel vskqrelylq qkilcelsgh inlfvdvnkh lfdgevcain hfvkllkdii
841 icflnirakn vaqnplkhhs ertdmktlsr khwssvqdyk cssfantssk frhllsndgy
901 pfk
HARBI1 (NP_776172)
1 maipitvldc dlllygrghr tldrfklddv tdeylmsmyg fprqfiyylv ellganlsrp
61 tqrsraispe tqvlaalgfy tsgsfqtrmg daigisqasm srcvanvtea lverasqfir
121 fpadeasiqa lkdefyglag mpgvmgvvdc ihvaikapna edlsyvnrkg lhslnclmvc
181 dirgtlmtve tnwpgslqdc avlqqsslss qfeagmhkds wllgdssffl rtwlmtplhi
241 petpaeyryn mahsathsvi ektfrtlcsr frcldgskga lqyspekssh iilaccvlhn
301 islehgmdvw sspmtgpmeq ppeeeyehme sldleadrir qelmlthfs
NAIF (NP_931045)
1 mavpakkrkm nfsereveii veelelkkhl lvnhfnagvp laaksaawhg ilrrvnavat
61 crrelpevkk kwsdlktevr rkvaqvraav eggeapgpte edgaggpgtg ggsggggpav
121 apvlltpmqq ricnllgeat iislpsttei hpvalgpsat aaaatvtltq iptettyhtl
181 eegvveycta eappplppet pvdmmaqhad tsvkpqalks rialnsakli qeqrvtnlhv
241 keiaqhleqq ndllqmirrs qevqacaqer qaqamegtqa alsvliqvlr pmikdfrryl
301 qsntanpapa sdpgqvaqng qpdsiiq
PGBD1 (NP_115896.1)
1 myealpgpap enedglvkvk eedptweqvc nsqegsshtq eicrlrfrhf cyqeahgpqe
61 alaqlrelch qwlrpemhtk eqimellvle qfltilpkel qpcvktyple sgeeavtvle
121 nletgsgdtg qqasvyiqgq dmhpmvaeyq gvslecqslq llpgittlkc eppqrpqgnp
181 qevsgpvphg sahlqeknpr dkavvpvfnp vrsqtlvkte eetaqavaae kwshlsltrr
241 nlcgnsaqet vmslspmtee ivtkdrlfka kqetseemeq sgeasgkpnr ecapqipcst
301 piatertvah lntlkdrhpg dlwarmhiss leyaagditr kgrkkdkarv sellqglsfs
361 gdsdvekdne peiqpaqkkl kvscfpeksw tkrdikpnfp swsaldsgll nlkseklnpv
421 elfelffdde tfnlivnetn nyasqknvsl evtvqemrcv fgvlllsgfm rhprremywe
481 vsdtdqnlvr dairrdrfel ifsnlhfadn ghldqkdkft klrplikqmn knfllyaple
541 eyycfdksmc ecfdsdqfln gkpirigyki wcgtttqgyl vwfepyqees tmkvdedpdl
601 glggnlvmnf advllergqy pyhlcfdsff tsvkllsalk kkgvratgti renrtekcpl
661 mnvehmkkmk rgyfdfriee nneiilcrwy gdgiislcsn avgiepvnev sccdadneei
721 pqisqpsivk vydeckegva kmdqiiskyr vrirskkwys ilvsymidva mnnawqlhra
781 cnpgasldpl dfrrfvahfy lehnahlsd
PGBD2 (NP_001017434.1)
1 mncnfqkhap leefysfges mceyfghrgs kqlhrgkpvr lgykiwcgtt srgylvwfep
61 sqgtlftkpd rsldlggsmv ikfvdalqer gflpyhiffd kvftsvklms ilrkkgvkat
121 gtvreyrter cplkdpkelk kmkrgsfdyk vdeseeiivc rwhdssvvni csnavgiepv
181 rltsrhsgaa ktrtqvhqps lvklyqekvg gvgrmdqnia kykvkirgmk wyssfigyvi
241 daalnnawql hriccqdaqv dllafrryia cvylesnadt tsqgrrsrrl etesrfdmig
301 hwiihqdkrt rcalchsqtn trcekcqkgv hakcfreyhi r
PGBD3 (NP_736609)
1 mprtlslhei tdlletddsi easaiviqpp enatapvsde esgdeeggti nnlpgsllht
61 aayliqdgsd aesdsddpsy apkddspdev pstftvqqpp psrrrkmtki lckwkkadlt
121 vqpvagrvta ppndfftvmr tpteilelfl ddevielivk ysnlyacskg vhlgltssef
181 kcflgiifls gyvsvprrrm fweqrtdvhn vlvsaamrrd rfetifsnlh vadnanldpv
241 dkfsklrpli sklnercmkf vpnetyfsfd efmvpyfgrh gckqfirgkp irfgykfwcg
301 atclgyicwf qpyqgknpnt kheeygvgas lvlqfsealt eahpgqyhfv fnnfftsial
361 ldklssmghq atgtvrkdhi drvplesdva lkkkergtfd yridgkgniv crwndnsvvt
421 vassgagihp lclvsrysqk lkkkiqvqqp nmikvynqfm ggvdradeni dkyrasirgk
481 kwysspllfc felvlqnawq lhktydekpv dflefrrrvv chylethghp pepgqkgrpq
541 krnidsrydg inhvivkqgk qtrcaechkn ttfrcekcdv alhvkcsvey hte
PGBD4 (NP_689808.2)
1 msnprkrsip mrdsntgleq llaedsfdes dfseiddsdn fsdsaleadk irplshlesd
61 gksstssdsg rsmkwsaram iprqrydftg tpgrkvdvsd itdplqyfel ffteelvski
121 tretnaqaal laskppgpkg fsrmdkwkdt dndelkvffa vmllqgivqk pelemfwstr
181 plldtpylrq imtgerflll frclhfvnns sisagqskaq islqkikpvf dflvnkfstv
241 ytpnrniavd eslmlfkgpl amkqylptkr vrfglklyvl cesqsgyvwn alvhtgpgmn
301 lkdsadglks srivltlvnd llgqgycvfl dnfnispmlf relhqnrtda vgtarlnrkq
361 ipndlkkria kgttvarfcg elmalkwcdg kevtmlstfh ndtvievnnr ngkktkrprv
421 ivdynenmga vdsadqmlts ypserkrhkv wykkffhhll hitvlnsyil fkkdnpehtm
481 shinfrlali ermlekhhkp gqqhlrgrpc sddvtplrls grhfpksipa tsgkqnptgr
541 ckiccsqydk dgkkirketr yfcaecdvpl cvvpcfeiyh tkkny
PGBD5 (NP_078830.2)
1 mpdyrtralf vqstlgdsag pelqllsivp grdlqpsdsf tassspppsv wselehirgk
61 seattelkrp slpgsihscl cgvwcgvgkr tpsslvsslq dlellgqrqr qlwmestprp
121 pynpflvpct pvgklsaehg gptrkmppsa savdffqlfv pdnvlknmvv qtnmyakkfq
181 erfgsdgawv evtltemkaf lgymistsis hcesvlsiws ggfysnrsla lvmsqarfek
241 ilkyfhvvaf rssqtthgly kvqpfldslq nsfdsafrps qtqvlhepli dedpvfiatc
301 terelrkrkk rkfslwvrqc sstgfiiqiy vhlkegggpd gldalknkpq lhsmvarslc
361 rnaagknyii ftgpsitslt lfeefekqgi yccgllrark sdctglplsm ltnpatppar
421 gqyqikmkgn mslicwynkg hfrfltnays pvqqgviikr ksgeipcpla veafaahlsy
481 icryddkysk yfishkpnkt wqqvfwfais iainnayily kmsdayhvkr ysraqfgerl
541 vrellgleda spth
Reliability of the nanopheres-DNA immunization technology to produce polyclonal antibodies directed against human neogenic proteins
Ahmed Arnaoty, Valérie Gouilleux, Sophie Casteret, Bruno Pitard,
Yves Bigot and Thierry Lecomte
Supplementary material 2 : DNA sequences of the pVAX plasmidsthat were used for the mice immunizations and the transient expressions in HeLa cells.
LOCUS GS50153-6 pVAX Seq6 ZBED1 5057 bp DNA CIRCULAR SYN 26-OCT-2010
DEFINITION Ligation of Seq6 ZBED1/EcoRI,XbaI into pVAX/EcoRI,XbaI
ACCESSION GS50153-6 pVAX Seq6 ZBED1
KEYWORDS .
SOURCE Unknown.
ORGANISM Unknown
Unclassified.
REFERENCE 1 (bases 1 to 5057)
AUTHORS Self
JOURNAL Unpublished.
COMMENT SECID/File created by SciEd Central, Scientific & Educational Software
FEATURES Location/Qualifiers
CDS 756..2850
/gene="'Seq6 ZBED1'"
BASE COUNT 1130 a 1399 c 1509 g 1019 t
ORIGIN
1 GACTCTTCGC GATGTACGGG CCAGATATAC GCGTTGACAT TGATTATTGA CTAGTTATTA
61 ATAGTAATCA ATTACGGGGT CATTAGTTCA TAGCCCATAT ATGGAGTTCC GCGTTACATA
121 ACTTACGGTA AATGGCCCGC CTGGCTGACC GCCCAACGAC CCCCGCCCAT TGACGTCAAT
181 AATGACGTAT GTTCCCATAG TAACGCCAAT AGGGACTTTC CATTGACGTC AATGGGTGGA
241 CTATTTACGG TAAACTGCCC ACTTGGCAGT ACATCAAGTG TATCATATGC CAAGTACGCC
301 CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT TATGCCCAGT ACATGACCTT
361 ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC ATCGCTATTA CCATGGTGAT
421 GCGGTTTTGG CAGTACATCA ATGGGCGTGG ATAGCGGTTT GACTCACGGG GATTTCCAAG
481 TCTCCACCCC ATTGACGTCA ATGGGAGTTT GTTTTGGCAC CAAAATCAAC GGGACTTTCC
541 AAAATGTCGT AACAACTCCG CCCCATTGAC GCAAATGGGC GGTAGGCGTG TACGGTGGGA
601 GGTCTATATA AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA
661 AATTAATACG ACTCACTATA GGGAGACCCA AGCTGGCTAG CGTTTAAACT TAAGCTTGGT
721 ACCGAGCTCG GATCCACTAG TCCAGTGTGG TGGAATTCGA AGGCATGGAG AACAAGAGCC
781 TGGAGAGCAG CCAGACCGAC CTGAAGCTGG TGGCCCACCC CAGGGCCAAG AGCAAGGTGT
841 GGAAGTACTT CGGCTTCGAC ACCAACGCCG AGGGCTGCAT CCTGCAGTGG AAGAAGATCT
901 ACTGCAGGAT CTGCATGGCC CAGATCGCCT ACAGCGGCAA CACCAGCAAC CTGAGCTACC
961 ACCTGGAGAA GAACCACCCC GAGGAGTTCT GCGAGTTCGT GAAGAGCAAC ACCGAGCAGA
1021 TGAGGGAGGC CTTCGCCACC GCCTTCAGCA AGCTGAAGCC CGAGAGCAGC CAGCAGCCCG
1081 GCCAGGACGC CCTGGCCGTG AAGGCCGGCC ACGGCTACGA CAGCAAGAAG CAGCAGGAGC
1141 TGACCGCCGC CGTGCTGGGC CTGATCTGCG AGGGCCTGTA CCCCGCCAGC ATCGTGGACG
1201 AGCCCACCTT CAAGGTGCTG CTGAAGACCG CCGACCCCAG GTACGAGCTG CCCAGCAGGA
1261 AGTACATCAG CACCAAGGCC ATCCCCGAGA AGTACGGCGC CGTGAGGGAG GTGATCCTGA
1321 AGGAGCTGGC CGAGGCCACC TGGTGCGGCA TCAGCACCGA CATGTGGAGG AGCGAGAACC
1381 AGAACAGGGC CTACGTGACC CTGGCCGCCC ACTTCCTGGG CCTGGGCGCC CCCAACTGCC
1441 TGAGCATGGG CAGCAGGTGC CTGAAGACCT TCGAGGTGCC CGAGGAGAAC ACCGCCGAGA
1501 CCATCACCAG GGTGCTGTAC GAGGTGTTCA TCGAGTGGGG CATCAGCGCC AAGGTGTTCG
1561 GCGCCACCAC CAACTACGGC AAGGACATCG TGAAGGCCTG CAGCCTGCTG GACGTGGCCG
1621 TGCACATGCC CTGCCTGGGC CACACCTTCA ACGCCGGCAT CCAGCAGGCC TTCCAGCTGC
1681 CCAAGCTGGG CGCCCTGCTG AGCAGGTGCA GGAAGCTGGT GGAGTACTTC CAGCAGAGCG
1741 CCGTGGCCAT GTACATGCTG TACGAGAAGC AGAAGCAGCA GAACGTGGCC CACTGCATGC
1801 TGGTGAGCAA CAGGGTGAGC TGGTGGGGCA GCACCCTGGC CATGCTGCAG AGGCTGAAGG
1861 AGCAGCAGTT CGTGATCGCC GGCGTGCTGG TGGAGGACAG CAACAACCAC CACCTGATGC
1921 TGGAGGCCAG CGAGTGGGCC ACCATCGAGG GCCTGGTGGA GCTGCTGCAG CCCTTCAAGC
1981 AGGTGGCCGA GATGCTGAGC GCCAGCAGGT ACCCCACCAT CAGCATGGTG AAGCCCCTGC
2041 TGCACATGCT GCTGAACACC ACCCTGAACA TCAAGGAGAC CGACAGCAAG GAGCTGAGCA
2101 TGGCCAAGGA GGTGATCGCC AAGGAGCTGA GCAAGACCTA CCAGGAGACC CCCGAGATCG
2161 ACATGTTCCT GAACGTGGCC ACCTTCCTGG ACCCCAGGTA CAAGAGGCTG CCCTTCCTGA
2221 GCGCCTTCGA GAGGCAGCAG GTGGAGAACA GGGTGGTGGA GGAGGCCAAG GGCCTGCTGG
2281 ACAAGGTGAA GGACGGCGGC TACAGGCCCG CCGAGGACAA GATCTTCCCC GTGCCCGAGG
2341 AGCCCCCCGT GAAGAAGCTG ATGAGGACCA GCACCCCCCC CCCCGCCAGC GTGATCAACA
2401 ACATGCTGGC CGAGATCTTC TGCCAGACCG GCGGCGTGGA GGACCAGGAG GAGTGGCACG
2461 CCCAGGTGGT GGAGGAGCTG AGCAACTTCA AGAGCCAGAA GGTGCTGGGC CTGAACGAGG
2521 ACCCCCTGAA GTGGTGGAGC GACAGGCTGG CCCTGTTCCC CCTGCTGCCC AAGGTGCTGC
2581 AGAAGTACTG GTGCGTGACC GCCACCAGGG TGGCCCCCGA GAGGCTGTTC GGCAGCGCCG
2641 CCAACGTGGT GAGCGCCAAG AGGAACAGGC TGGCCCCCGC CCACGTGGAC GAGCAGGTGT
2701 TCCTGTACGA GAACGCCAGG AGCGGCGCCG AGGCCGAGCC CGAGGACCAG GACGAGGGCG
2761 AGTGGGGCCT GGACCAGGAG CAGGTGTTCA GCCTGGGCGA CGGCGTGAGC GGCGGCTTCT
2821 TCGGCATCAG GGACAGCAGC TTCCTGTAAT CTAGAGGGCC CGTTTAAACC CGCTGATCAG
2881 CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT
2941 TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC
3001 ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG
3061 AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG GCTTCTACTG
3121 GGCGGTTTTA TGGACAGCAA GCGAACCGGA ATTGCCAGCT GGGGCGCCCT CTGGTAAGGT
3181 TGGGAAGCCC TGCAAAGTAA ACTGGATGGC TTTCTCGCCG CCAAGGATCT GATGGCGCAG
3241 GGGATCAAGC TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG
3301 ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA
3361 ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT
3421 TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAAGACG AGGCAGCGCG
3481 GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA
3541 AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA
3601 CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT
3661 TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC
3721 TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC
3781 GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC GACGGCGAGG ATCTCGTCGT
3841 GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT
3901 CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG
3961 TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT
4021 CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAAT
4081 TATTAACGCT TACAATTTCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC
4141 ACACCGCATA CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT
4201 TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA
4261 TAATAGCACG TGCTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT
4321 GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC
4381 GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG
4441 CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT
4501 CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG