LOCUS Exported File 4311 bp ds-DNA circular SYN 18-APR-2013
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS pENTR223.1-LC
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4311)
AUTHORS Transomic
TITLE Direct Submission
JOURNAL Exported Thursday, Apr 18, 2013 from SnapGene 1.5.2
FEATURES Location/Qualifiers
source 1..4311
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 864..891
/note="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
/note="color: #ffffff"
terminator 983..1069
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
/note="color: #ffffff"
primer_bind 1133..1149
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
/note="color: #a020f0; direction: RIGHT"
CDS complement(1312..1617)
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/note="ccdB"
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/note="color: #993366"
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
CDS complement(1959..2618)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/note="color: #ccffcc"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
promoter complement(2619..2721)
/note="cat promoter"
/note="promoter of the E. coli cat gene"
/note="color: #ffffff; direction: LEFT"
promoter complement(2903..2921)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
/note="color: #ffffff; direction: LEFT"
primer_bind complement(2926..2942)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
/note="color: #a020f0; direction: LEFT"
CDS 3373..4164
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase"
/note="SmR"
/note="confers resistance to spectinomycin and
streptomycin"
/note="color: #ccffcc"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 4257..534
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
ORIGIN
1 ctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaact
61 ggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccac
121 cacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtg
181 gctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccg
241 gataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcga
301 acgacctacaccgaactgagatacctacagcgtgagcattgagaaagcgccacgcttccc
361 gaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacg
421 agggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctc
481 tgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgcc
541 agcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttcttt
601 cctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgatacc
661 gctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgc
721 ccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgac
781 aggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatacgcgtaccgctagc
841 caggaagagtttgtagaaacgcaaaaaggccatccgtcaggatggccttctgcttagttt
901 gatgcctggcagtttatggcgggcgtcctgcccgccaccctccgggccgttgcttcacaa
961 cgttcaaatccgctcccggcggatttgtcctactcaggagagcgttcaccgacaaacaac
1021 agataaaacgaaaggcccagtcttccgactgagcctttcgttttatttgatgcctggcag
1081 ttccctactctcgcgttaacgctagcatggatgttttcccagtcacgacgttgtaaaacg
1141 acggccagtcttaagctcgggccccaaataatgattttattttgactgatagtgacctgt
1201 tcgttgcaacaaattgatgagcaatgcttttttataatgccaactttgtacaaaaaagca
1261 gaagggccgtcaaggccctgcagactggctgtgtataagggagcctgacatttatattcc
1321 ccagaacatcaggttaatggcgtttttgatgtcattttcgcggtggctgagatcagccac
1381 ttcttccccgataacggagaccggcacactggccatatcggtggtcatcatgcgccagct
1441 ttcatccccgatatgcaccaccgggtaaagttcacgggagactttatctgacagcagacg
1501 tgcactggccagggggatcaccatccgtcgcccgggcgtgtcaataatatcactctgtac
1561 atccacaaacagacgataacggctctctcttttataggtgtaaaccttaaactgcatttc
1621 accagtccctgttctcgtcagcaaaagagccgttcatttcaataaaccgggcgacctcag
1681 ccatcccttcctgattttccgctttccagcgttcggcacgcagacgacgggcttcattct
1741 gcatggttgtgcttaccagaccggagatattgacatcatatatgccttgagcaactgata
1801 gctgtcgctgtcaactgtcactgtaatacgctgcttcatagcacacctctttttgacata
1861 cttcgggtatacatatcagtatatattcttataccgcaaaaatcagcgcgcaaatacgca
1921 tactgttatctggcttttagtaagccggatccacgcgattacgccccgccctgccactca
1981 tcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacagacggca
2041 tgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgccc
2101 atggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtg
2161 aaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaa
2221 taggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccgg
2281 aaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacg
2341 gtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgg
2401 aattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttg
2461 tgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggtta
2521 taggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggat
2581 atatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaa
2641 aatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttg
2701 gaacctcttacgtgccgatcaacgtctcattttcgccaaaagttggcccagggcttcccg
2761 gtatcaacagggacaggcctcatgggcccagctttcttgtacaaagttggcattataaaa
2821 aataattgctcatcaatttgttgcaacgaacaggtcactatcagtcaaaataaaatcatt
2881 atttgccatccagctgatatcccctatagtgagtcgtattacatggtcatagctgtttcc
2941 tggcagctctggcccgtgtctcaaaatctctgatgttacattgcacaagataaaaatata
3001 tcatcatgcctcctctagaccagccaggacagaaatgcctcgacttcgctgctgcccaag
3061 gttgccgggtgacgcacaccgtggaaacggatgaaggcacgaacccagtggacataagcc
3121 tgttcggttcgtaagctgtaatgcaagtagcgtatgcgctcacgcaactggtccagaacc
3181 ttgaccgaacgcagcggtggtaacggcgcagtggcggttttcatggcttgttatgactgt
3241 ttttttggggtacagtctatgcctcgggcatccaagcagcaagcgcgttacgccgtgggt
3301 cgatgtttgatgttatggagcagcaacgatgttacgcagcagggcagtcgccctaaaaca
3361 aagttaaacatcatgagggaagcggtgatcgccgaagtatcgactcaactatcagaggta
3421 gttggcgtcatcgagcgccatctcgaaccgacgttgctggccgtacatttgtacggctcc
3481 gcagtggatggcggcctgaagccacacagtgatattgatttgctggttacggtgaccgta
3541 aggcttgatgaaacaacgcggcgagctttgatcaacgaccttttggaaacttcggcttcc
3601 cctggagagagcgagattctccgcgctgtagaagtcaccattgttgtgcacgacgacatc
3661 attccgtggcgttatccagctaagcgcgaactgcaatttggagaatggcagcgcaatgac
3721 attcttgcaggtatcttcgagccagccacgatcgacattgatctggctatcttgctgaca
3781 aaagcaagagaacatagcgttgccttggtaggtccagcggcggaggaactctttgatccg
3841 gttcctgaacaggatctatttgaggcgctaaatgaaaccttaacgctatggaactcgccg
3901 cccgactgggctggcgatgagcgaaatgtagtgcttacgttgtcccgcatttggtacagc
3961 gcagtaaccggcaaaatcgcgccgaaggatgtcgctgccgactgggcaatggagcgcctg
4021 ccggcccagtatcagcccgtcatacttgaagctagacaggcttatcttggacaagaagaa
4081 gatcgcttggcctcgcgcgcagatcagttggaagaatttgtccactacgtgaaaggcgag
4141 atcaccaaggtagtcggcaaataaccctcgagccacccatgaccaaaatcccttaacgtg
4201 agttacgcgtcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttga
4261 gatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccacc g
//