Current applications of wheat and wheat-alien precise genetic stocks(by Elena K. Khlestkina)
Supplementary Table 1.Examples of recent applications of wheat precise genetic stocks and wheat-alien lines.
Lines (reference) / Application / ReferenceNullitetrasomic and/or ditelosomic lines
Nullitetrasomic (Sears, 1953) and/or ditelosomic (Sears, 1944, 1946) lines of bread wheat ‘Chinese Spring’ (‘CS’)1 / Purifying individual chromosome arms of wheat using flow sorting, production of chromosome arm-specific BAC libraries and obtaining chromosome arm-specific sequences / Gill et al. 1999; Kubaláková 2002; Guoet al. 2006; Jandaet al. 2006; She et al. 2008; Safár 2010; Berkmanet al. 2011, 2012; Wicker et al. 2011; Hernandez et al. 2012; Akhunov et al. 2013; Tanaka et al. 2013
Chromosomal location of wheat DNA-markers / Chao et al. 1989; Röder et al. 1995, 1998; Huang et al. 2000; Somers et al. 2003; Qi et al. 2004; Sourdille et al. 2004
Chromosomal location of wheat ESTs / Qi et al. 2004
Chromosomal location of wheat genic sequences / Li et al. 1999; Himi and Noda 2004; Boisson et al. 2005; Nomuraet al. 2005; Applefordet al. 2006; Shitsukawaet al. 2007; Khlestkina et al. 2008, 2010а
Chromosomal location of wheat genes on proteomic platform / Islam et al. 2002
Identification of specific homoeologues / Shoevaet al. 2014
Studying homoeologous gene expression in wheat / Bottley et al. 2006
Deletion lines
Deletion lines of bread wheat ‘CS’ (Endo and Gill 1996)1 / Physical mapping of wheat DNA-markers / Sourdille et al. 2004
Physical mapping of wheat ESTs / Qi et al. 2004
Physical mapping of wheat genic sequences / Himi and Noda 2004; Boisson et al. 2005; Nomura et al. 2005; Khlestkina et al. 2008; Shoevaet al. 2014
Physical mapping of wheat genes on proteomic platform / Islam et al. 2003; Ma et al. 2012
Wheat-alien addition lines
Wheat-rye addition lines T. aestivumCS+Secalecereale ‘Imperial’
(Driscoll and Sears 1971)1,2 / Purifying individual chromosome arms of rye using flow sorting, production of chromosome arm-specific BAC libraries and obtaining chromosome arm-specific sequences / Kubaláková et al. 2003; Martis et al. 2013
chromosomal location of rye DNA-markers / Chao et al. 1989; Khlestkina et al. 2004; Bolibok-Brągoszewskaet al. 2009; Benito et al. 2010
Chromosomal location of rye genic sequences / Khlestkinaet al. 2009а; Khlestkina and Shoeva 2014
Identifying the regulatory activity of certain rye genes / Khlestkina, 2010
Wheat-barley addition lines T. aestivum CS +Hordeum vulgare ‘Betzes’ (Islam et al. 1981; Islam 1983)1,2 / Purifying individual chromosome arms of barley using flow sorting; production of chromosome arm-specific BAC libraries and obtaining chromosome arm-specific sequences / Suchánkováet al. 2006; Mayer et al. 2011
Chromosomal location of barley DNA-markers / Chao et al. 1989; Pillenet al. 2000; Cho et al. 2006; Bilgicet al. 2007
Wheat-Aegilops addition lines
T. aestivum ‘CS’+Aegilops speltoides (Friebe et al. 2000) 1
T. aestivum ‘CS’+Ae. longissima(Friebe et al. 1993) 1
T. aestivum‘CS’+Ae. searsii (Friebeet al. 1995)1 / Chromosomal location of Ae.speltoides DNA-markers / Dobrovolskaya et al. 2011
Identifying the regulatory activity of certain Aegilops spp. genes / Khlestkina 2010
T. aestivum+Haynaldiavillosa(Ying and Chen 2000)3 / Chromosomal location ofH.villosagenic sequences and identification of chromosome-specific DNA-markers / Zhang et al. 2006; Cao et al. 2011
T. aestivum+Psathyrostachys huashanica (Du et al. 2013a, b)4 / Identification of chromosome-specific DNA-markers / Du et al. 2013a, b
Supplementary Table 1. (continuation)
Lines (reference) / Application / ReferenceWheat-alien chromosome substitution lines
Wheat-rye substitution line T. aestivum ‘Saratovskaya 29’(S.cereale ‘Onokhoiskaya’) (Silkovaet al. 2006)5 / Assessment of activity of certain non-wheat genes at wheat background / Khlestkina et al. 2009а
Wheat-barley substitution line T. aestivum ‘Pyrothrix 28’(H. marinum)
(Trubacheevaet al. 2008)5 / Khlestkina 2010
Wheat-Aegilops substitution line T. aestivum ‘CS’(Ae. tauschii) (McFadden and Sears 1947)2
Intervarietal singlechromosome substitution lines
‘CS’(‘Hope’) (Kuspira and Unrau 1958)1 / Determining role of the genes underpinning pigmentation of different parts of wheat plant in flavonoid biosynthesis regulatory network; studying homoeologous gene expression in wheat / Himi et al. 2005; Khlestkina et al. 2008, 2010b; Khlestkina 2010
‘Saratovskaya 29’(Yanetzkis Probat) (Gaidalenok et al. 2005)5
Single chromosome recombinant lines (SCRLs)
T. aestivum ‘CS’(‘Cheyenne’ 5B)/‘CS’, ‘Hobbit Sib’(‘CS’ 5BL)/ ‘Hobbit Sib’ (Tóthet al. 2003)6 / Detection of QTLs for flowering time and frost resistance / Tóthet al. 2003
T. aestivum ‘Favorit’(‘F26-70’ 7B)/ ‘Favorit’ (Giura and Ittu 1986; Khlestkina et al. 2009b)2 / Mapping genes for photoperiod response and grain protein content / Khlestkina et al. 2009b
T. aestivum ‘CS’(T. spelta 7D)/‘CS’
(Simon et al. 2005, 2010)2 / Detection of QTLs for septoria resistance / Simónet al. 2010
Introgression lines (ILs)
T. aestivum‘Saratovskaya 29’-T. timopheevii (Leonova et al. 2004)5 / Determining precise positions of the genes underpinning traits or encoding enzymes / Leonova et al. 2008; Tereshchenko, 2012
T. aestivum ‘Alcedo’-Ae. markgrafii (Iqbal et al. 2007)2 / Iqbal et al. 2007
T. aestivum ‘CS’-Ae. tauschii (Pestsova et al. 2006)2 / Pestsova et al. 2006
Determining role of the genes underpinning pigmentation of different parts of wheat plant in flavonoid biosynthesis regulatory network / Khlestkina et al. 2008; Khlestkina, 2010
T. aestivum-H. villosa T6VS-6AL (Chen et al. 1995)3 / Identification and precise location of the candidate gene for the powdery mildew resistance gene Pm21 / Cao et al. 2011
T. aestivum ‘Saratovskaya 29’-T. timopheevii/ T. aestivum ‘Rodina’-Ae.speltoides
(Tereshchenkoet al. 2012а)5 / Finding novel genes by their complementary effect in the IL / Tereshchenko et al. 2012а
Near-isogenic lines (NILs)
NILs of ‘Saratovskaya 29’ (Arbuzovaet al. 1998) and ‘Novosibirskaya 67’ (Koval, 1997)2, 5 / Determining precise positions of the genes underpinning pigmentation of different parts of wheat plant / Khlestkina et al. 2000; Tereshchenko et al. 2012b
Determining role of the genes underpinning pigmentation of different parts of wheat plant in the flavonoid biosynthesis regulatory network / Himi et al. 2005; Khlestkina 2010; Tereshchenko et al. 2013
NILs of ‘Bezostaya 1’ (Efremovaet al. 2011)5 / Establishing functional differences between Vrn (vernalization sensitivity) alleles and homoeoalleles / Shcherbanet al. 2013
NILs of ‘Triple Dirk’ (Pugsley 1971, 1972)7 / Loukoianovet al. 2005
NILs of ‘Century’ (Carver et al. 1993)8 / Establishing gene networks involved into biotic and abiotic stress resistance by transcriptome profiling of the NILs / Houde and Diallo 2008
NILs of ‘Sumai’/‘Stoa’ (Pumphreyet al. 2007)9 / Jiaet al. 2009
NILs of ‘Thatcher’ (Dyck and Samborski 1968)10 / Hulbert et al. 2007; Bolton et al. 2008; Manickavelu 2010
NILs of ‘98B69*L47’ and ‘BW30’ (Hamzehzarghaniet al. 2008; Gunnaiahet al. 2012)11 / Studying metabolic pathways/ gene networks involved into fusarium head blight resistance by metabolome and/or proteome profiling of the NILs / Hamzehzarghaniet al. 2008; Gunnaiahet al. 2012
1Wheat Genetics Resource Center of the Kansas State University (Manhattan, USA)
2Genbank of the Leibniz Institute for Plant Genetics and Crop Plant Research (Gatersleben, Germany)
3Cytogenetics Institute of Nanjing Agricultural University (Nanjing, China)
4Genetic Engineering for Plant Breeding, College of Agronomy, Northwest A&F University (Shaanxi, China)
5Institute of Cytology and Genetics SB RAS (Novosibirsk, Russia)
6John Innes Centre (Norwich, UK)
7Washington State University (Pullman, USA)
8Oklahoma State University (Stillwater, USA)
9University of Minnesota (St. Paul, USA)
10Kihara Institute for Biological Research, Yokohama City University (Yokohama, Japan)
11McGill University (Quebec, Canada)
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