Worksheet for Mutation Exploration

WORKSHEET FOR MUTATION EXPLORATION

I. CHART A

ORIGINAL DNA
5’-3’ DNA
3’-5 DNA / T / A / C / T / G / A / G / C / T / G / A / G / C / T / T / C / A / G / A / G / C / A / C / T
5’-3’ mRNA
AMINO ACIDS
MUTATION-EXAMPLE 1
3’-5’ DNA
mRNA
AMINO ACIDS
MUTATION-EXAMPLE-2
3’-5’ DNA
mRNA
AMINO ACIDS
MUTATION-EXAMPLE 3
3’-5’ DNA
mRNA
AMINO ACIDS
MUTATION-EXAMPLE 4
3’-5’ DNA
mRNA
AMINO ACIDS
MUTATION-EXAMPLE 5
3’-5’ DNA
mRNA
AMINO ACIDS
MUTATION-EXAMPLE 6
3’-5’ DNA
mRNA
AMINO ACIDS

II. QUESTIONS FOR ROMAN NUMERAL III EXPLORING MUTATIONS I

A. Mutation-Example 1

Did this change in the DNA sequence cause any significant change to the protein? Explain

B. Mutation-Example 2

Did this change in the DNA sequence cause any change to the protein? Explain

C. Mutation-Example 3

Did this change in the DNA sequence cause any significant change to the protein? Explain

III. QUESTIONS FOR ROMAN NUMERAL IV EXPLORING MUTATIONS IV

A.  Mutation-Example 4

Did this change in the DNA sequence cause any significant change to the protein produced? Explain

B.  Mutation-Example 5

Did this change in the DNA sequence cause any significant change to the protein produced? Explain

What is worse, an insertion of an extra base toward the beginning of the DNA instructions or toward the end? Explain

C.  Mutation-Example 6

Did this change in the DNA sequence cause any significant change to the protein produced? Explain

What is worse, a deletion of a base toward the beginning of the DNA instructions or toward the end? Explain

IV. CHART B

Type of point mutation / Definition / Consequence / Example
Mutation example 1
Mutation example 2
Mutation example 3
Mutation example 4
Mutation example 5
Mutation example 6

TYPES OF POINT MUTATION:

a.  Missense

b.  Silent

c.  Frameshift

d.  Nonsense

DEFINITIONS:

a.  Base substituted, does not change amino acid

b.  Base substitution, changes amino acid

c.  Base substitution, results in early stop codon

d.  Addition or deletion of a base

CONSEQUENCES:

a.  Reading frame shifted-massive missense

b.  Change in primary structure of protein

c.  Premature termination-protein not completed

d.  Change in genotype but no change to phenotype

EXAMPLES:

THE FAT CAT ATE THE RAT (NORMAL)

THE FAT BAT ATE THE RAT

THE FAT STOP

THE ATC ATA TET HER AT

THE GFA TBA TAT ETH ERA T

THE (PH)AT CAT ATE THE RAT