IACUC PROTOCOL Reviewuniversity of Rhode Island

IACUC PROTOCOL REVIEWUniversity of Rhode Island

CHECKLIST FOR REQUIRED CONTENT Office of Research Integrity

This checklist is used by IACUC member review to ensure completion of all required protocol elements with respect to applicable regulations, policies, and guidelines.

FOR REVIEWERS: Check to indicate if the proposal contains the required information, provide comments, and upload on IRBnet.org.

GENERAL INFORMATION (SECTIONS 1 THROUGH 6)
1. / Personnel conducting procedures on the species being maintained or studied are appropriately qualified and trained in those procedures; / Yes No NA
2. / A rationale for involving animals, and for the appropriateness of the species to be used is provided; / Yes No NA
3. / The PI has provided written assurance that the activities do not unnecessarily duplicate previous experiments; / Yes No NA
4. / The PI has provided a justification based on statistical methods or scientific rationale for the number of animals to be used; / Yes No NA
5. / A complete description of the proposed use of the animals; / Yes No NA
6. / Identification of the species and the approximate number of animals to be used; / Yes No NA
7. / The appropriate USDA pain category has been identified. / Yes No NA
PAIN AND/OR DISCOMFORT (SECTION 7)
8. / A description of procedures designed to assure that discomfort and pain to animals will be limited to that which is unavoidable for the conduct of scientifically valuable research, / Yes No NA
9. / including provision for the use of analgesic, anesthetic, and tranquilizing drugs where indicated and appropriate to minimize discomfort and pain to animals; / Yes No NA
10. / Procedures avoid or minimize discomfort, distress, and pain to the animals; / Yes No NA
11. / Procedures that may cause more than momentary or slight pain or distress to the animals will: / Yes No NA
11a /

• Be performed with appropriate sedatives, analgesics or anesthetics,

/ Yes No NA
11b /

• Withholding such agents is justified for scientific reasons, in writing, by the PI and will continue for only the necessary period of time;

/ Yes No NA
11c /

• Involve, in their planning, consultation with the attending veterinarian;

/ Yes No NA
11d /

• Not include the use of paralytics without anesthesia;

/ Yes No NA
12. / Animals that would otherwise experience severe or chronic pain or distress that cannot be relieved will be painlessly euthanized at the end of the procedure or, if appropriate, during the procedure; / Yes No NA
ANIMAL HUSBANDRY (SECTION 8)
13. / The animals' living conditions are appropriate for their species in accordance with 9 CFR Part 3, and contribute to their health and comfort. / Yes No NA
14. / The housing, feeding, and nonmedical care of the animals will be directed by the attending veterinarian or other scientist trained and experienced in the proper care, handling, and use of the species being maintained or studied; / Yes No NA
15. / The PI is requesting deviations from The Guide which must be voted upon by IACUC members / Yes No NA
ALTERNATIVES (SECTION 9)
16. / The PI has considered alternatives (i.e., reduce, refine or replace) to procedures that may cause more than momentary or slight pain or distress, and has provided a written narrative description of the methods and sources used to determine that alternatives were not available; / Yes No NA
OTHER PROCEDURES (SECTION 10)
17. / If restraints will be used, is it well described and scientifically justified. / Yes No NA
SURGICAL PROCEDURES (SECTION 11)
18. / Activities that involve surgery include appropriate provision for postoperative care in accordance with established veterinary medical and nursing practices / Yes No NA
19. / All survival surgery will be performed using aseptic procedures, including surgical gloves, masks (when working with regulated species), sterile instruments, and aseptic techniques. / Yes No NA
20. / Major operative procedures on non-rodents will be conducted only in facilities intended for that purpose which shall be operated and maintained under aseptic conditions. / Yes No NA
21. / Non-major operative procedures and all surgery on rodents do not require a dedicated facility, but must be performed using aseptic procedures. / Yes No NA
22. / Operative procedures conducted at field sites not performed in dedicated facilities, are performed using aseptic procedures; / Yes No NA
23. / Animal will not be used in more than one major operative procedure from which it is allowed to recover, unless: / Yes No NA
23a /

• Justified for scientific reasons by the principal investigator, in writing.

/ Yes No NA
23b /

• Required as routine veterinary procedure or to protect the health as determined by the attending veterinarian; or

/ Yes No NA
23c /

• In other special circumstances as determined by the USDA Administrator on an individual basis.

/ Yes No NA
HAZARDOUS MATERIALS (SECTION 12)
24. / The safety of the personnel’s working environment has beenassessed. / Yes No NA
25. / IBC review has been conducted / Yes No NA
FINAL DESPOSITION (SECTION 15)
26. / Description of euthanasia method to be used. / Yes No NA
27. / Method of euthanasia is in accordance with the definition of the term set forth in 9 CFR Part 1, § 1.1 and AVMA Guidelines for Euthanasia, unless / Yes No NA
27a /

• A deviation is justified for scientific reasons, in writing, by the investigator.

/ Yes No NA
GENERAL COMMENTS

REFERENCES

AVMA Guidelines for the Euthanasia of Animals: 2013 Edition /
USDA Regulations 9CFR Subchapter A, Part 2, Subpart C; /
PHS Policy on Humane Care and Use of Laboratory Animals /
Guide for the Care and Use of Laboratory Animals, 8th Edition (2011) /
US Government Principles for the Utilization and Care of Vertebrate Animals used in Testing, Research, and Training; /