Detection and Mapping of Qtls Affecting Contents of Pharmaceutical Alkaloids in Leaf and Root
Detection and mapping of QTLs affecting contents of pharmaceutical alkaloids in leaf and root
of Catharanthus roseus
Swati Chaudhary1,2, Richa Pandey1, Vishakha Sharma1, Bhumi Nath Tripathi2 and Sushil Kumar1*
1Genetical Genomics Laboratory, National Institute of Plant Genome Research (NIPGR),
Aruna Asaf Ali Marg, New Delhi-110067, India
2Banasthali University, PO Banasthali Vidhyapeeth, Rajasthan 304022, India
*Corresponding author
Professor Sushil Kumar
Email: ,
Telephone: 91-11-26735177; 91-11-26865494
Fax: 91-11-26741658
Table S1 Details of the 6 EST-SSR markers mapped on the linkage map of C. roseus.
PrimerIDa / Accession No. / Primer sequence
( 5´ 3´) / SSR motifs / Tm (ºC) / BLAST X / Parental originb / Amplified fragment size (bp) / LG on which marker located
CrES361 / gi|116660141| / (F) CTTATTCCTCCGACAGTCATCTG
(R) ACCCCTTCTTTCATCTCTTCTTTC / (AT)12 / 60.5 / Catharanthus roseus cDNA clone CR04006F03 5 / DP / 180 / LG 1
CrES364 / gi|116660113| / (F) CTATCCGTCTCTCCTCCTTTCTCT
(R) TAGACACCCATCATCAACAATAGC / (GA)6 / 60.0 / Catharanthus roseus cDNA clone CR04006C12 5' / lli / 220 / LG 2
CrES377 / gi|116659333| / (F) CCCTTTAAGAGAAGAGAAGGGAAC
(R) GCTTTTGACATAGCAGAGCTGTAG / (AG)11 / 58.3 / Catharanthus roseus cDNA clone CR03016B11 5' / lli / 250 / LG 5
CrES390 / gi|116658437| / (F) AAGCCGTAAACAAATTCGTCCT
(R) TCTTCCTCTCTTCGGCTATAAAAC / (TA)6 / 59.5 / Catharanthus roseus cDNA clone CR03035A06 5' / DP / 250 / LG 8
CrES392 / gi|116658280| / (F) TGGTGGTTCTACAGCAGAAATAAG
(R) TTGGTCCATTTCATTCACCAG / (GA)20 / 59.5 / Catharanthus roseus cDNA clone CR03033A09 5' / DP / 300 / LG 1
CrES401 / gi|116657425| / (F) CTTTTCAATCTCTCTTCTGGGGTA
(R) GTCATCTCCTGCCTTAGGTGCT / (AG)10 / 60.0 / Catharanthus roseus cDNA clone CR03021C07 5' / DP / 250 / LG 1
a The other primer pairs that were tested but whose markers did not get mapped were: CrES360 - CrES380, CrES390 - CrES405 (Chaudhary et al. 2011)
b DP= ‘Delhi Pink’; ‘lli’= ‘gsr-8’