Detection and mapping of QTLs affecting contents of pharmaceutical alkaloids in leaf and root

of Catharanthus roseus

Swati Chaudhary1,2, Richa Pandey1, Vishakha Sharma1, Bhumi Nath Tripathi2 and Sushil Kumar1*

1Genetical Genomics Laboratory, National Institute of Plant Genome Research (NIPGR),

Aruna Asaf Ali Marg, New Delhi-110067, India

2Banasthali University, PO Banasthali Vidhyapeeth, Rajasthan 304022, India

*Corresponding author

Professor Sushil Kumar

Email: ,

Telephone: 91-11-26735177; 91-11-26865494

Fax: 91-11-26741658

Table S1 Details of the 6 EST-SSR markers mapped on the linkage map of C. roseus.

Primer
IDa / Accession No. / Primer sequence
( 5´ 3´) / SSR motifs / Tm (ºC) / BLAST X / Parental originb / Amplified fragment size (bp) / LG on which marker located
CrES361 / gi|116660141| / (F) CTTATTCCTCCGACAGTCATCTG
(R) ACCCCTTCTTTCATCTCTTCTTTC / (AT)12 / 60.5 / Catharanthus roseus cDNA clone CR04006F03 5 / DP / 180 / LG 1
CrES364 / gi|116660113| / (F) CTATCCGTCTCTCCTCCTTTCTCT
(R) TAGACACCCATCATCAACAATAGC / (GA)6 / 60.0 / Catharanthus roseus cDNA clone CR04006C12 5' / lli / 220 / LG 2
CrES377 / gi|116659333| / (F) CCCTTTAAGAGAAGAGAAGGGAAC
(R) GCTTTTGACATAGCAGAGCTGTAG / (AG)11 / 58.3 / Catharanthus roseus cDNA clone CR03016B11 5' / lli / 250 / LG 5
CrES390 / gi|116658437| / (F) AAGCCGTAAACAAATTCGTCCT
(R) TCTTCCTCTCTTCGGCTATAAAAC / (TA)6 / 59.5 / Catharanthus roseus cDNA clone CR03035A06 5' / DP / 250 / LG 8
CrES392 / gi|116658280| / (F) TGGTGGTTCTACAGCAGAAATAAG
(R) TTGGTCCATTTCATTCACCAG / (GA)20 / 59.5 / Catharanthus roseus cDNA clone CR03033A09 5' / DP / 300 / LG 1
CrES401 / gi|116657425| / (F) CTTTTCAATCTCTCTTCTGGGGTA
(R) GTCATCTCCTGCCTTAGGTGCT / (AG)10 / 60.0 / Catharanthus roseus cDNA clone CR03021C07 5' / DP / 250 / LG 1

a The other primer pairs that were tested but whose markers did not get mapped were: CrES360 - CrES380, CrES390 - CrES405 (Chaudhary et al. 2011)

b DP= ‘Delhi Pink’; ‘lli’= ‘gsr-8’