Defective Folliculogenesis in Female Mice Lacking Vaccinia-Related Kinase 1

Defective folliculogenesis in female mice lacking Vaccinia-related kinase 1

Jinkyung Kim1,2ŧ, Yoon Ha Choi3ŧ, Soeun Chang1,4, Kyong-TaiKim3,5*, and Jung Ho Je1,2,4*

1X-ray Imaging Center, Pohang University of Science and Technology (POSTECH), Pohang, 70-784, South Korea. 2School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), Pohang, 790-784, South Korea. 3Department of Life Science, Division of Molecular and Life Sciences, Pohang University of Science and Technology (POSTECH), Pohang, 790-784, South Korea. 4Department of Materials Science and Engineering, Pohang University of Science and Technology (POSTECH), Pohang, 790-784, South Korea. 5Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology(POSTECH), Pohang, 790-784, South Korea.

ŧThese authors contributed equally to this work.

*Correspondence and requests for materials should be addressed to J.H.J. (; +82-54-279-2143/fax +82-54-279-2992), K.T.K. (; +82-54-279-2297/fax +82-54-279-2199)

Supplementary Fig. S1

Effect of VRK1-deficiency onantrum formation. (a) Schematic of a Graffian follicle with the oocyte (black), the granulosa/cumulus cells (orange), and the antrum cavity. (b) Comparison of the antrum cavity volume of Graffian follicles in normal and VRK1-deficient mice with various ages (age 6, 16, 43 weeks). Sample number is shown in bar. The errors correspond to the s.e.m..

SupplementaryTable 1 The follicle volumes in normal and VRK1-deficient mice with various ages.

Supplementary Table 2PCR primer sequences

Supplementary movielegends

Supplementary Movie1

Microtomographic movie of a wholeovaryin normal mouse.

Supplementary Movie2

Segmented microtomographic movie obtained bythe Supplemental Movie 1(Fig.1a).navy, pre-antral follicle; green, antral follicle; violet,Graffian follicle; yellow, corpus luteum.

Supplementary Movie3

Transparentmicrotomographic movie of an antral follicle in normal ovaryshows oocyte and their antral structure (Fig.1c).

Supplementary Movie4

Transparentmicrotomographic movie of a Graffian follicle in normal ovaryshows oocyte and their antral structure (Fig.1d).

Supplementary Movie5

Segmented microtomographic movie obtained by the Supplemental Movie 5(Fig.2a).navy, pre-antral follicle; green, antral follicle; violet,Graffian follicle; yellow, corpus luteum; black; ruptured follicle.

Supplementary Movie6

Microtomographic movie of a wholeovaryinVRK1-deficient mouse.

Supplementary Movie7

Transparentmicrotomographic movie of an antral follicle in VRK1-deficientovaryshows oocyte and their antral structure (Fig.2b).

Supplementary Movie8

Transparentmicrotomographic movie of a Graffian follicle in VRK1-deficientovaryshows oocyte and their antral structure (Fig.2c).

Supplementary Movie9

Segmented microtomographic movie of the ruptured follicle in VRK1-deficient ovary(Fig.2d).