Curriculum vitae

Dr. Claire PEGHAIRE–born February 15, 1986
Research associate, National Heart and Lung Institute, Imperial College London

Skills

-angiogenic models in vivo: management mouse lines, model of post-natal vascularisation of mouse retina, model of retinopathy induced by oxygen
-endothelial cells culture (HUVEC, HMVEC, EAhy, MS1), culture under flow
-study of endothelial cells properties (migration, permability, proliferation, apoptosis, sprouting in 3D matrix, adhesion).
-molecular biology (transfection, siRNA, extraction of RNA, RT-qPCR, PCR, Western blot, interaction protein-protein by Immunoprecipitation, Proximity Ligation Assay, fractioning, ChIP-qPCR, ChIP-seq basic analysis)
-microscopy, data analysis and statistical procedures: immunostaining, confocal microscopy, softwares (Zen, Axiovision, ImageJ, Imaris, Volocity, UCSC gene Browser, GraphPad Prism, Excel)
-ability to work independently or as part of a team, to supervise students and technical staff and to drive the preparation of scientific papers for publication.

Academic formation

2004-2015: Doctorate of Pharmacy (PharmD), with honours, University of Bordeaux, France
2011-2014: PhD in Cellular biology, physiology and pathology, with honours, University of Bordeaux, France
2010-2011: Master in Cellular biology, physiology and pathology, with honours, University of Bordeaux, France
2001-2004: High School Diploma (french baccalaureate) with advanced Science, with honours, Pau, France

Work experiences

Current Employment (Since July 2015): Reasearch Associate, National Heart and Lung Institute, Imperial College London, Prof. Anna Randi Group, UK (1 year and 6 months)
2015: Engineer position, INSERM Unit 1034 “Cardiovascular adaptation to ischemia”, Pessac, France (6 months)
2012-2014: PhD Fellowship: Characterization of the role of Frizzled7 receptor in vascular integrity and angiogenesis, INSERM Unit 1034 “Cardiovascular adaptation to ischemia”, Pessac, France (3 years and 3 months)
2012: Tutoring-Teaching activity in Biochemistry, Faculty of Pharmaceutical Sciences, University of Bordeaux, France (1 year)
2011: Internship. Master in Cellular biology, physiology and pathology, Characterization of the role of Frizzled7 in vessels formation, INSERM Unit 1034 “Cardiovascular adaptation to ischemia”, Pessac, France (6 months)
2010: Traineeship. INSERM Unit 828 “Cardiovascular adaptation to ischemia”, Pessac, France (3 months)
2010: Pharmacist Externship. Saint André Hospital, Bordeaux, France. (8 months)
2008: Traineeship. INSERM Unit 885 «Pharmacology: pulmonary vascular physiology and pathology team», Bordeaux, France (6 weeks)

Membership of Professional Bodies

-Member of French Angiogenesis Society (SFA)
-Member of French Cardiology Society (SFC)
-Member of Study Group on Cardiovascular Research (GRRC)

Publications

- Dufton N, Peghaire C, Osuna-Almagro L, Kalna V, Chuacan A, Webb G, Yang Y, Birdsey GM, Lalor P, Mason JC, Adams D and Randi AM. Dynamic regulation of canonical TGFβ signaling by the endothelial transcription factor ERG protects from liver fibrogenesis. Nature Comm. 2016; Submitted.
- Shah AV, Birdsey GM, Peghaire C, Pitulescu ME, Dufton N, Yang Y, Weinberg I, Osuna Almagro L, Payne L, Mason JC, Gerhardt H, Adams RH and Randi AM. Angiopoietin-1-dependent control of Notch signalling and vascular stability is mediated by the ETS transcription factor ERG. Nat Commun. 2016; Submitted.
-Peghaire C, Bats ML, Sewduth RN, Jeanningros S, Jaspard B, Couffinhal T, Duplaa C, Dufourcq P. Fzd7 expressed by endothelial cells controls blood vessel formation through Wnt/β-catenin canonical signalling. ATVB 2016, ATVBAHA.116.307926.
-Guillabert-Gourges A, Jaspard B, Bats ML, Sewduth RN, Franzl N, Peghaire C, Jeanningros S, Moreau C, Roux E, Larrieu-Lahargue F, Dufourcq P, Couffinhal T, Duplaa C. Kif26b controls endothelial cell polarity through the Dishevelled/Daam1-dependent planar cell polarity-signaling pathway. Mol Biol Cell. 2016; 27(6)
-Sewduth RN, Jaspard-Vinassa B, Peghaire C, Guillabert A, Franzl N, Larrieu-Lahargue F, Moreau C, Fruttiger M, Dufourcq P, Couffinhal T and Duplàa C. The ubiquitin ligase PDZRN3 is required for vascular morphogenesis through Wnt/planar cell polarity signalling. Nat Commun. 2014; 5:4832.
-Ferreira Tojais N, Peghaire C, Franzl N, Larrieu-Lahargue F, Jaspard B, Moreau C, Couffinhal T, Duplàa C and Dufourcq P. Frizzled7 controls vascular permeability through the Wnt canonical pathway and cross-talk with endothelial cell junction complexes. Cardiovasc Res 2014, 133.

Communications

Oral communication
-Peghaire C, Sewduth RN, Couffinhal T, Duplaa C, Dufourcq P: Frizzled7 is required for post-natal angiogenesis. 3rd International Meeting of French Angiogenesis Society 2011; Bordeaux (France).
Posters with Published Abstract
- Bats ML, Peghaire C, Duplaa C, Couffinhal T, Dufourcq P: Role of during pathological angiogenesis: a model of retinopathy. PC0269, Printemps de la cardiologie, GRRC, Toulouse (France). Archives of cardiovascular diseases 2015, 07-2S.
-Peghaire C, Bats ML, Couffinhal T, Duplaa C, Dufourcq P: Frizzled7 controls postnatal vascular formation through Wnt/β-catenin canonical signaling in a Dll4/Notch dependent and independent way. PC0048, Printemps de la cardiologie, GRRC, Toulouse (France). Archives of cardiovascular diseases 2015, 07-2S.
Posters
-Shah, AV , Birdsey, GM, Peghaire, C, Pitulescu, ME, Yang, Y, Weinberg I , Osuna Almagro, L, Dufton N, Mason, JC, Gerhardt, H, Adams, RH and Randi, AM. The ETS transcription factor ERG is a key player in Angiopoietin-1-dependent control of Notch signalling and vascular stability, Christmas London Vascular Biology Forum 2016; London (UK). Poster Prize.
-Shah, AV, Birdsey, GM, Peghaire, C, Pitulescu, ME, Yang, Y, Weinberg I, Osuna Almagro, L, Dufton N, Mason, JC, Gerhardt, H, Adams, RH and Randi, AM. The ETS transcription factor ERG is a key player in Angiopoietin-1-dependent control of Notch signalling and vascular stability, BHF Centre of Research Excellence Annual Symposium 2016; London (UK). Poster Prize.
-Peghaire C, Bats ML, Couffinhal T, Duplaa C, Dufourcq P: Frizzled7 controls post-natal angiogenesis in a Notch dependent and independent way. 5th International Meeting of French Angiogenesis Society 2014; Chamonix (France). EACR/SFA Poster Prize.
-Ferreira Tojais N, Peghaire C, Larrieu-Lahargue F, Couffinhal T, Duplàa C, Dufourcq, P: Fzd7 controls vascular permeability through the Wnt canonical pathway and cross-talk with endothelial cell junction complexes. Gordon Conference 2013; Los Angeles (USA).
-Ferreira Tojais N, Peghaire C, Larrieu-Lahargue F, Couffinhal T, Duplàa C, Dufourcq, P: Fzd7 regulates endothelial cell junctions. International Vascular Biology Meeting 2012; Wiesbaden (Germany).