ELECTRONIC SUPPLEMENTARY MATERIAL
CROCUS SATIVUS L. GENOMICS AND DIFFERENT DNA BARCODE APPLICATIONS
Angelo Gismondi, Federica Fanali, Juan Manuel Martínez Labarga1, Maria Grilli Caiola and Antonella Canini
Dept. of Biology, University of Rome “Tor Vergata”, Via della Ricerca Scientifica 1 – 00133 - Roma, ITALY
1Departamento de Producción Vegetal: Botánica y Protección Vegetal,Escuela de Ingeniería Forestal y del Medio Natural (EIFORNAT), Ramiro de Maeztu s/n,Madrid - E-28.040.Universidad Politécnica de Madrid (UPM), SPAIN
Corresponding author:
Antonella Canini
Dept. of Biology
University of Rome “Tor Vergata”
Via della Ricerca Scientifica 1, 00133, Roma, ITALY
Phone: +39 06 5279 4344
e-mail:
ELECTRONIC SUPPLEMENTARY MATERIAL LEGEND
Lists of accessions, registered in NCBI (Nucleotide Scientific Databank, that matched with nucleotide sequence obtained by ITS barcode gene PCR amplification (Fig. 2) of DNA extracted from commercial saffron sachet DNA.
Accession, Description, Max score,Total score, Query coverage, E-value, Max identity, Links
DQ094185.2Crocus sativus 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence 1077 1077 100% 0.0 98%
AB699586.1Crocus sativus genes for 18S rRNA, ITS1, 5.8S rRNA, ITS2, 26S rRNA, partial and complete sequence 1072 1072 100% 0.0 98%
HE801161.1Crocus oreocreticus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher MAIC:3674 1040 1040 100% 0.0 97%
HE801144.1Crocus sativus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7277 1018 1018 100% 0.0 96%
HE801118.1Crocus thomasii genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher CLU 13102 1014 1014 100% 0.0 96%
HE801133.1Crocus pallasii subsp. pallasii genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7230 918 918 100% 0.0 94%
HE801126.1Crocus asumaniae genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7207 881 881 100% 0.0 93%
HE801090.1Crocus mathewii genomic DNA containing ITS1, putative 5.8S rRNA pseudogene and ITS2, specimen voucher GAT:7189, clone c 876 876 100% 0.0 92%
HE801089.1Crocus mathewii genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7189, clone d 861 861 100% 0.0 92%
HE801088.1Crocus mathewii genomic DNA containing ITS1, putative 5.8S rRNA pseudogene and ITS2, specimen voucher GAT:7189, clone h 850 850 100% 0.0 92%
HE801160.1Crocus cartwrightianus var. creticus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher MAIC:1991 845 845 100% 0.0 92%
HE801172.1Crocus gilanicus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7286 778 778 99% 0.0 90%
HE801086.1Crocus mathewii genomic DNA containing ITS1, putative 5.8S rRNA pseudogene and ITS2, specimen voucher GAT:7189, clone e 774 774 98% 0.0 90%
HE801083.1Crocus mathewii genomic DNA containing ITS1, putative 5.8S rRNA pseudogene and ITS2, specimen voucher GAT:7189, clone i 774 774 98% 0.0 90%
HE801148.1Crocus kotschyanus subsp. kotschyanus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7287 773 773 99% 0.0 90%
HE801153.1Crocus karduchorum genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7398 769 769 99% 0.0 89%
HE801135.1Crocus scharojanii genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7233 769 769 99% 0.0 89%
HE801132.1Crocus ochroleucus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7228 765 765 99% 0.0 89%
HE801158.1Crocus sieberi subsp. nivalis genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7409 741 741 98% 0.0 89%
HE801087.1Crocus mathewii genomic DNA containing ITS1, putative 5.8S rRNA pseudogene and ITS2, specimen voucher GAT:7189, clone g 739 739 100% 0.0 89%
HE801137.1Crocus dalmaticus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7253 736 736 98% 0.0 89%
HE801134.1Crocus robertianus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7231 721 721 98% 0.0 88%
HE801168.1Crocus vallicola genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7234 719 719 99% 0.0 88%
HE801155.1Crocus veluchensis genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7400 713 713 98% 0.0 88%
HE801169.1Crocus kosaninii genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7258 706 706 99% 0.0 88%
HE801152.1Crocus ilvensis genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7397 706 706 99% 0.0 88%
HE801150.1Crocus etruscus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7394 706 706 99% 0.0 88%
HE801146.1Crocus nudiflorus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7280 704 704 100% 0.0 88%
HE801156.1Crocus vernus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7402 697 697 99% 0.0 88%
HE801122.1Crocus vernus subsp. albiflorus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7199 697 697 99% 0.0 88%
AM503892.1Crocus vernus ITS1 (partial), 5.8S rRNA gene, ITS2 and 28S rRNA gene (partial) 697 697 99% 0.0 88%
HE801147.1Crocus banaticus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7285 695 695 99% 0.0 88%
HE801131.1Crocus imperati genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7221 695 695 100% 0.0 88%
HE801170.1Crocus malyi genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7259 693 693 99% 0.0 88%
HE801125.1Crocus serotinus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7206 691 691 99% 0.0 88%
DQ224364.1Crocus vernus 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence 691 691 99% 0.0 88%
HE801108.1Crocus cf. biflorus subsp. tauri GAT 7174 genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7174, clone HKEP0929c 688 688 98% 0.0 87%
HE801167.1Crocus ligusticus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7226 686 686 99% 0.0 87%
HE801081.1Crocus niveus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7197, clone g 686 686 98% 0.0 87%
HE801077.1Crocus niveus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7197, clone c 686 686 98% 0.0 87%
DQ224363.1Crocus vernus 18S ribosomal RNA gene, partial sequence; and internal transcribed spacer 1, 5.8S ribosomal RNA gene, internal transcribed spacer 2, and 26S ribosomal RNA gene, complete sequence 686 686 99% 0.0 87%
HE801119.1Crocus olivieri genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7135 684 684 98% 0.0 87%
HE801111.1Crocus cf. biflorus subsp. tauri GAT 7174 genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7174, clone HKEP0929f 682 682 98% 0.0 87%
HE801110.1Crocus cf. biflorus subsp. tauri GAT 7174 genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7174, clone HKEP0929e 682 682 98% 0.0 87%
HE801078.1Crocus niveus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7197, clone h 680 680 98% 0.0 87%
HE801154.1Crocus olivieri subsp. istanbulensis genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7399 678 678 98% 0.0 87%
HE801174.1Crocus hadriaticus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher StH 1532 676 925 94% 0.0 96%
HE801165.1Crocus longiflorus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7223 676 676 99% 0.0 87%
HE801162.1Crocus almehensis genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher TARI<IRN>:69170 676 676 98% 0.0 87%
HE801143.1Crocus serotinus subsp. salzmannii genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7276 675 675 99% 0.0 87%
HE801097.1Crocus corsicus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7252, clone g 675 675 99% 0.0 87%
HE801096.1Crocus corsicus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7252, clone f 675 675 99% 0.0 87%
HE801082.1Crocus niveus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7197, clone j 675 675 98% 0.0 87%
HE801080.1Crocus niveus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7197, clone e 675 675 98% 0.0 87%
HE801076.1Crocus niveus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7197, clone a 675 675 98% 0.0 87%
HE801075.1Crocus reticulatus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7141, clone HKEP0919d 673 673 98% 0.0 87%
HE801072.1Crocus reticulatus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7141, clone HKEP0919a 671 671 98% 0.0 87%
HE801136.1Crocus angustifolius genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7236 669 669 98% 0.0 87%
HE801060.1Crocus hyemalis genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7118, clone b 669 669 98% 0.0 86%
HE801121.1Crocus biflorus subsp. biflorus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7198 667 667 98% 0.0 87%
HE801095.1Crocus corsicus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7252, clone e 665 665 99% 0.0 87%
HE801074.1Crocus reticulatus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7141, clone HKEP0919c 665 665 98% 0.0 87%
HE801092.1Crocus corsicus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7252, clone b 662 662 99% 0.0 87%
HE801091.1Crocus corsicus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7252, clone a 662 662 99% 0.0 87%
HE801079.1Crocus niveus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7197, clone i 658 658 98% 0.0 86%
HE801057.1Crocus hyemalis genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7118, clone d 656 656 98% 0.0 86%
HE801109.1Crocus cf. biflorus subsp. tauri GAT 7174 genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7174, clone HKEP0929d 654 654 98% 0.0 86%
HE801093.1Crocus corsicus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7252, clone c 654 654 99% 0.0 86%
HE801073.1Crocus reticulatus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7141, clone HKEP0919b 654 654 98% 0.0 86%
HE801141.1Crocus pestalozzae genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7261 652 652 98% 0.0 86%
HE801142.1Crocus versicolor genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7270 643 643 99% 0.0 86%
HE801130.1Crocus goulimyi genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7217 643 643 98% 0.0 86%
HE801107.1Crocus cf. biflorus subsp. tauri GAT 7174 genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7174, clone HKEP0929b 643 643 98% 0.0 86%
HE801106.1Crocus cf. biflorus subsp. tauri GAT 7174 genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7174, clone HKEP0929a 643 643 98% 0.0 86%
HE801094.1Crocus corsicus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7252, clone d 643 643 99% 0.0 86%
HE801127.1Crocus boryi genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7209 636 636 98% 7e-179 86%
HE801123.1Crocus tournefortii genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7202 636 636 98% 7e-179 86%
HE801173.1Crocus hartmannianus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7473 634 634 98% 2e-178 86%
HE801128.1Crocus cancellatus subsp. pamphylicus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7213 625 625 98% 1e-175 86%
HE801104.1Crocus cambessedesii genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7211, clone d 625 625 99% 1e-175 86%
HE801103.1Crocus cambessedesii genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7211, clone c 625 625 99% 1e-175 86%
HE801159.1Crocus kerndorffiorum genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher HKEP9010 621 621 98% 2e-174 86%
HE801145.1Crocus pulchellus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7278 619 619 98% 7e-174 86%
HE801129.1Crocus cancellatus subsp. cancellatus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7215 619 619 98% 7e-174 86%
HE801102.1Crocus cambessedesii genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7211, clone f 616 616 99% 9e-173 85%
HE801101.1Crocus cambessedesii genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7211, clone g 616 616 99% 9e-173 85%
HE801100.1Crocus cambessedesii genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7211, clone h 616 616 99% 9e-173 85%
HE801099.1Crocus cambessedesii genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7211, clone b 616 616 99% 9e-173 85%
HE801098.1Crocus cambessedesii genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7211, clone a 616 616 99% 9e-173 85%
HE801105.1Crocus cambessedesii genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7211, clone i 610 610 99% 4e-171 85%
HE801120.1Crocus speciosus subsp. ilgazensis genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7196 608 608 98% 1e-170 85%
HE801163.1Crocus hermoneus subsp. palaestinus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher IABH:7 603 603 98% 7e-169 85%
HE801124.1Crocus speciosus subsp. xantholaimos genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7204 603 603 98% 7e-169 85%
HE801066.1Crocus carpetanus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7250, clone d 599 599 99% 9e-168 84%
HE801065.1Crocus carpetanus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7250, clone c 599 599 99% 9e-168 84%
HE801064.1Crocus carpetanus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7250, clone b 599 599 99% 9e-168 84%
HE801067.1Crocus carpetanus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7250, clone f 597 597 99% 3e-167 84%
HE801071.1Crocus carpetanus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7250, clone a 592 592 99% 1e-165 84%
HE801070.1Crocus carpetanus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7250, clone e 592 592 99% 1e-165 84%
HE801068.1Crocus carpetanus genomic DNA containing ITS1, 5.8S rRNA gene and ITS2, specimen voucher GAT:7250, clone h 592 592 99% 1e-165 84%