Conservation of Queen Pheromones Across Two Species of Vespine Wasps

Conservation of Queen Pheromones Across Two Species of Vespine Wasps

Conservation of Queen Pheromones Across Two Species of Vespine Wasps

Authors: Cintia A. Oi1, Jocelyn G. Millar2, Jelle S. van Zweden1, and Tom Wenseleers1*

Authors affiliations:
1Laboratory of Socioecology and Social Evolution, KU Leuven, Naamsestraat 59, 3000 Leuven, Belgium

2Departments of Entomology and Chemistry, University of California, Riverside CA, 92521, USA

*Corresponding author: , telephone +32 16 32 39 64

Supplemental Material

Table S1 Identification of cuticular compounds of Dolichovespula saxonica. RT: Retention Time, RI: Retention Index.

Peak # / RT / RI / Identification / Peak # / RT / RI / Identification
1 / 13.13 / 2293 / 5-C23:1 / 34 / 22.72 / 2830 / 14-, 13-, 12-, 11-MeC28
2 / 13.21 / 2300 / n-C23 / 35 / 22.89 / 2839 / 6-MeC28
3 / 13.72 / 2334 / 11-, 9-MeC23 / 36 / 23.24 / 2856 / 4-MeC28
4 / 13.81 / 2340 / 7-MeC23 / 37 / 23.64 / 2876 / 9-C29:1
5 / 14.30 / 2372 / 3-MeC23 / 38 / 23.79 / 2883 / 7-C29:1 and 4,8-diMeC28
6 / 14.73 / 2400 / n-C24 / 39 / 24.01 / 2894 / 5-C29:1
7 / 14.85 / 2408 / 3,7-diMeC23 / 40 / 24.13 / 2900 / n-C29
8 / 15.97 / 2474 / 9-C25:1 / 41 / 24.71 / 2929 / 15-, 13-, 11-, 9-MeC29
9 / 16.22 / 2489 / 4,8-diMeC24 / 42 / 24.91 / 2938 / 7-MeC29
10 / 16.42 / 2500 / n-C25 / 43 / 25.10 / 2948 / 5-MeC29
11 / 17.05 / 2535 / 9-MeC25 / 44 / 25.22 / 2954 / 13,17-, 11,15-diMeC29
12 / 17.14 / 2540 / 7-MeC25 / 45 / 25.30 / 2958 / 9,13-diMeC29
13 / 17.29 / 2549 / 5-MeC25 / 46 / 25.44 / 2965 / 7,11-diMeC29
14 / 17.73 / 2573 / 3-MeC25 / 47 / 25.59 / 2972 / 3-MeC29
15 / 17.84 / 2579 / 5,9-diMeC25 / 48 / 25.72 / 2979 / 9-C30:1
16 / 18.23 / 2600 / n-C26 / 49 / 26.14 / 3000 / n-C30
17 / 18.34 / 2606 / 3,9-diMeC25 / 50 / 26.25 / 3005 / 3,7-diMeC29
18 / 19.31 / 2657 / 4-MeC26 / 51 / 26.43 / 3014 / Unidentified
19 / 19.66 / 2675 / 9-C27:1 / 52 / 26.70 / 3028 / 15, 14-, 13-, 12-, 8-, 6-MeC30
20 / 19.81 / 2683 / 7-C27:1 / 53 / 27.25 / 3056 / 4-MeC30
21 / 19.91 / 2688 / 4,8- diMeC26 / 54 / 27.67 / 3077 / 9-C31:1
22 / 20.03 / 2694 / 5-C27:1 / 55 / 27.80 / 3084 / 7-C31:1
23 / 20.15 / 2700 / n-C27 / 56 / 27.98 / 3093 / cholesterol
24 / 20.77 / 2732 / 13-, 11-, 9-MeC27 / 57 / 28.12 / 3100 / n-C31
25 / 20.92 / 2740 / 7-MeC27 / 58 / 28.67 / 3127 / 15-, 13-, 11-MeC31
26 / 21.10 / 2749 / 5-MeC27 / 59 / 28.79 / 3133 / 9-MeC31
27 / 21.28 / 2758 / 11,15-diMeC27 / 60 / 28.89 / 3138 / 7-MeC31
28 / 21.45 / 2767 / 7,11-diMeC27 / 61 / 29.17 / 3152 / 5-MeC31 and 11,15-diMeC31
29 / 21.58 / 2773 / 3-MeC27 / 62 / 29.57 / 3172 / 3-MeC31
30 / 21.68 / 2778 / 5,9- diMeC27 / 63 / 30.09 / 3200 / n-C32
31 / 21.79 / 2784 / 5,13-diMeC27 / 64 / 31.62 / 3276 / 9-C33:1
32 / 22.11 / 2800 / n-C28 / 65 / 32.03 / 3300 / n-C33
33 / 22.24 / 2807 / 3,7-diMeC27

Table S2 Compounds in bold were selected to be tested in bioassays in the wasp Dolichovespula saxonica. Compounds were ranked by the mean fold difference based on log2 transforms of the relative peak areas of queens vs. workers (rpa = relative peak area; Q = queens; W = workers; LCL = lower confidence limit; UCL = upper confidence limit).

Peak # / Identification / Fold difference / t ratio / P value / padj / rpaQ / rpaQ LCL / rpaQ UCL / rpaW / rpaW LCL / rpaW UCL
62 / 3-MeC31 / 3.676 / 5.087 / 0.000 / 0.000 / 0.020 / 0.012 / 0.034 / 0.005 / 0.003 / 0.009
50 / 3,7-diMeC29 / 3.530 / 4.851 / 0.000 / 0.000 / 0.024 / 0.013 / 0.043 / 0.007 / 0.004 / 0.013
47 / 3-MeC29 / 2.781 / 3.850 / 0.001 / 0.002 / 0.093 / 0.056 / 0.157 / 0.034 / 0.025 / 0.045
57 / n-C31 / 2.766 / 3.706 / 0.001 / 0.002 / 0.029 / 0.017 / 0.050 / 0.011 / 0.008 / 0.014
54 / 7-C31:1 / 2.144 / 5.457 / 0.000 / 0.000 / 0.038 / 0.028 / 0.053 / 0.018 / 0.012 / 0.025
40 / n-C29 / 1.975 / 2.571 / 0.016 / 0.025 / 0.188 / 0.112 / 0.314 / 0.095 / 0.071 / 0.127
49 / n-C30 / 1.686 / 4.632 / 0.000 / 0.000 / 0.013 / 0.010 / 0.017 / 0.008 / 0.006 / 0.010

Table S3 (a) Total of workers with undeveloped and developed ovaries in the control and in the treatment with blend of compounds: n-C29, 3-MeC29, n-C30, n-C31, and 3-MeC31. (b) Values of Z and P-values of the bioassays with the wasp Dolichovespula saxonica.

(a) / undeveloped / developed
Control / 121 / 32
Blend / 196 / 27
(b) / Estimate / SE / z value / P value
(intercept) / -1.33 / 0.27 / -4.83 / 1.33E-06 ***
Blend / -0.64 / 0.31 / -2.07 / 0.03*