SUPPLEMENTARY MATERIAL
27 AMINO ACID SEQUENCES IN FASTA FORMAT: CLOTTING AND RELATED PROTEINS FROM TWO SPECIES OF LAMPREY (LJ = LETHENTERON JAPONICUM AND PM = PETROMYZON MARINUS}
- FERROXIDASE FAMILY
COAGULATION FACTOR V (PARTIAL) (CHIMERA OF PM AND LJ)
CERULOPLASMINS (PM, LJ)
HEPHAESTIN-1 (LJ, PM)
HEPHAESTIN-2 (PM, LJ)
- VITAMIN K-DEPENDENT PRE-PROTEASES
PROTHROMBIN (PM, LJ)
PROTEIN C (PM, LJ)
FACTOR XA1 (PM, LJ)
FACTOR XA2 (LJ)
FACTOR XB (PM, LJ)
FACTOR VIIA (PM, LJ)
FACTOR VIIB (PM, LJ)
FACTOR VIIC (PM)
- FIBRINOGEN CHAINS
GAMMA CHAIN (PM, LJ)
BETA CHAIN (PM, LJ)
ALPHA CHAIN (PM, LJ)
D. TISSUE FACTOR (PM, LJ)
>CERULOPLASMIN PRECURSOR, PETROMYZON MARINUS
MHLAGMWWCV FYLNVAFTCA MMREYYIAAK EIYWDYAPSG RNLIKNISIS
DDEYQAILTL QRGPERIGRI YKKAVYIQYT DGSFTQEIPK DPWLGYLGPT
MKAEVHDVIA VYFKNLASRP YSMHPHGVFY SKASEGANYP DGQEFGSKGE
FVLPGQTHTY HWVVRDETSP GSDDPNCFTW IYHSHVNEPK DVNSGLIGAL
LTCRKGLLLG TAERQDVGED FILMFTIVDE NNSWYLEDNI RETIADPRSL
DRSDLHFQRS NFKTGINGYM YGNLPGLNMC SEVNVSWHIF GLGSEVDIHT
AHFRGQALTS KHHRVDTVGL FPATFVTALM VPTNPGRWLL ACDVNEHFEG
MQAAFNVLEC QGSNQSVWHS DGEERTFFIA AEEVDWDYGP SGINNATGED
LTQPGDSEKY FTKGPSRIGG VYRKILYTEY TDASFTARRQ RTPKQRHMGM
LGPVLRAEVG DELVVTFKNM GSRGYNIHPH GVFASSEHVA KGNYELHKVA
AVPAGGMHTY RWAVPEGAGP TQADEPCITY LYTSSVDHVR DGHSGLVGPL
LICRNGSLDQ HGDQGTDEEV FLLFDVVNEG LSWYLPWNKQ RNNVTAPDDD
SSFREANLRH AINGFMFGNL PGLEVCVGKR VSWHVLAVGS QADLHTAYFT
GQTLLFDLRR VDTIALFPHL SYTALMLTDT TGGFELMCRT NEHFNEGMRA
KFRVSECGMP PGPVFVPQRE RTYYLAIREE VWDYAPNRSW EMKMHNTTNS
PANKFLGSGE TRIGSRYQKV RFVEFTDDSF KNPKVRTTEE EHLAALGPLL
KGQVGELLVV VLWNRASRPY SIHSHGVISD VPGGLPLKPA GARKTYRWQI
PDRSGPSQAD TNCITWIYYS AVDFVRDPNS GLVGPLVVCR SGLLDDSGHR
NDVAHEFALL FMVYDENLSW YLRDNIDNFT GNPGAVDVAD KDFRESNLMH
GINGMTFGNL NGLNMGLKER VDWYLMGLGG ELDMHTIHFH GQTFRYKAGK
DTVSDVYDVF AGTFVTVEMT TDNPGTWLLH CHVHDHIEAG MVAFFQIKTN
>CERULOPLASMIN PRECURSOR, LETHENTERON JAPONICUM (SCAFFOLD 00377)a
MHLAGMWWCV FYLNVAFTCA MMREYYIAAK EIYWDYAPSG RNLIKNISIS
DDEYQARLTL QRGPERIGRI YKKAVYIQYT DGSFTQEIPK DPWLGYLGPT
IKAEVHDVIA VYFKNLASRP YSMHPHGVFY SKASEGANYP DGQEFGSKGE
FVLPGQTHTY HWVVRDETSP GSDDPNCFTW IYHSHVNEPK DVNSGLIGAL
LTCRKGLLLG TAERQDVGED FILMFTIVDE NNSWYLEDNI RETIADPRSL
DRSDPHFQRS NFKSSINGYM YGNLPGLNMC SEVNVSWHLF GLGSEVDIHT
AHFRGQALTS KHHRVDTVGL FPATFVTALM VPTNPGRWLL ACDVNEHFEG
GMQAAFNVLE CEGTNQSVWH SEGEERTFFI AAEEVDWDYG PSGVNNATGE
DLTQPGDSEK YFTKGPSRIG GVYRKILYTE YTDANFTTRR QRTPKQSHMG
MLGPVLRAEV GDEIVVTFKN LGSRGYNIHP HGVFASNEHV AKGSYELHKV
AAVPAGGMHT YRWAVPQGAG PTQADEPCIT YLYTSSVNHV RDGHSGLVGP
LLICRNGSLD QHGDQGTDEE VFLLFDVVDE SLSWYLQWNK QQNNVTAPDD
DPSFREANLR HAINGFMFGN LPGLEVCLGK RVSWHVLAVG SQADLHTAYF
TGQTLLFDLR RVDTIALFPH LSYTALMLTD TTGGFELMCR TNEHFNEGMR
AKFRVSECGM PPGPVFVPQK ERTYYLAIRE EVWDYAPNRS WEMKVHNTTN
SPANKFLGSG ETRIGSRYKK VRFVEYTDDS FKNPKVRTAE EEHLAALGPL
LKGQVGELLV VVLWNTASRP YSIHSHGVIS DVPGGLPLKP AGARKAYRWQ
IPERSGPSQA DTNCITWIYY SAVDFVRDPN SGLVGPLVVC RSGLLDDSGR
RDDVAHEFAL LFMVYNENLS WYLRDNIDNF TGNPGAVDVT DEDFRESNLM
HGINGMTFGN LNGLNMGLKE RVDWYLMGLG GELDMHTIHF HGQTFRYKAG
KDTVSDVYDV FAGTFVTVEM TTDNPGTWLL HCHVHDHIEA GMVAFFQIKT
>HEPHAESTIN-1 PRECURSOR, PETROMYZON MARINUS(GAP BASED ON L. JAPONICUMa)
MRPLQWLAVA CCWCSTWAPA APAVVRTHFI AIREIDWDYA PARRNLIADV
SVDDDEHAGT FVRRGPQRVG TVYTKAVYEE YADAGYSLAL PKEPWLGFLG
PVLRAEVGDT VVVHLRNLAS RPYTIHSHGL HYDKGSEGAP YPDHTERQQK
ADDAVAPGES REYTWRLKPE YGPMEADPAC ITWTYHSHVD APRDIAAGLI
GPLLACKKGS LDEHGNPQDP DRAFFLLFSV MDENLSWYLQ RNVERFCEQP
ATVDVNDEDF VESNKMHAIN GYVYGNAPRL ELCAGERVWW HLVAMGNEAD
VHAAHFLGHT LEERGRRVDT VALFPATFRS ARMAPARVGT WLISCQVNDH
VQGMMMLYTV RDCGLKPPAP VSGGVTRTFF VAAHEEVWDY APGGRDRISG
TKLDEPGSPS AVFLERGPHR VGSRYWKARY VAYEDDVFSA VRQRGKNDEH
LALLGPVLRV EVGDVLQVVF WNNASRPYSI QPHGVSYLKE HEGAPGTAGT
RAGGESVAPG RRFLYRWVVG EESGPPGGDP DCVTWLYYSA TDSVRKTNSV
LVGPLIVCRP GKLGADGAQR GIDHEFSLLF SVFDENLSWY LERNIAAFAS
DPAGVNRDDD DFQESNKMHG ------
------SS LTATMSADKA GDFGVVCKTA
DHLSAGMKQH YHVSLCDARV LRPPRPTATR THYVAAVELT WDYSPSRHWE
LARHNTTWQH SHGETFLRGG TDRIGSAYKK VVYREFTDDT FTREKPRGER
EKHLGIMGPM LRAEVGEALV VVFKNMASRP YNMHAHGVDE GLDSNVVEFV
EKYKWMVPER SGPGKGDPDC LLWAYYSNVD PNKDLYSGLF GSLLTCRSGV
LSGGGGDGAA RPRGVEREFA LLWMVFDENE SWYLEENVQR CCGGAAINSS
DEDFKESNKM HAINGYVYGN LPGLTMREGE HVAWYLLGMG NEVDIHTVHF
HGHPFTVHSG GTHRADVMDL FPATFETAHM RPGNPGTWLM HCHVTDHIQA
GMEATYTVLP RGTAPNEVSP NSTTPSSRIV MLYFKGSNAE RHAKLKRGTA
RKATPFPPVI SNAVFVYL
>HEPHAESTIN-1 PRECURSOR, LENTENTERON JAPONICUM (SCAFFOLD 00131a)
MRPLAWLAVA CCCCTWAPAA PAVVRTHFIA IREVDWDYAP ARRNLIADVS
VDDDEHAGTF MRRGPRRVGT VYTKAVYEEY ADAAFSLALP KEPWLGFLGP
VLRAEVGDTV VVHLRNLASR PYTIHSHGLH YDKSSEGAPY PDHTERHQQA
DDAVAPGESR EYTWSLKPEY GPTEADPACI TWTYHSHVDA PRDIAAGLIG
PLLACKKGSL DEHGNPQDPD RAFFLLFSVM DENLSWYLQR NVERFCEQPA
TVDVNDEDFV ESNKMHAING YLYGNAPRLE LCAGERVWWH LVAMGNEADV
HAAHFLGHTL EERGRRVDTV ALFPATFRSA RMAPARVGTW LISCQVNDHV
QGMMMLYTVRDCGLKPPAPVSGGVTRTFFVAAHEEVWDYAPGGRDRISGT
KLDEPGSPSAVFLERGPHRVGSRYWKARYVAYEDDVFSAVRQRGKNDEHL
ARAGPVLRVE VGDVLQVLFW NNASRPYSIQ PHGVSYLKQH EGPNPAGTRA
GGESVAPGRR FLYRWVVGEE SGPAGNDPDC VTWLYYSATD SVRDTNSGLV
GPLIVCRPGK LGADGAQRGI DHEFSLLFSV FDENLSWYLE RNIAAFASDP
AGVNRDDDDF QESNKMHGIN GRMYNTLQGL RMCKAQQVSW HLLGLGSEVD
VHAVYLAGNT MRASGRRRDT INLFPHPASL PLFPPPPPGD FGVVCKTADH
LSAGMKQHYH VSLCDARVLR PLRPTATRTH YVAAVELTWD YSPSRHWELA
RHNTTRQHRS HGETFLRGGT DRIGSAYKKV VYREFTDDTF TREKPRGERD
KHLGIMGGPM LRAEVGEALV VVFKNMASRP YNMHAHGVDE GLDSVEKYKW
MVPERSGPGK GDPDCLLWAY YSNVNPNKDL YSGLFGSLLT CRPGVLSGGG
SGDGAARPRG VEREFALLWM VFDENESWYL EENVQRCCGG AGVITSDEDF
KESNKMHAIN GYVYGNLPGL TMREGEHVAW YLLGMGNEVD IHTVHFHGHP
FTVGGTHRAD VMDLFPATFE TAHMRPGNPG TWLMHCHVTD HIQAGMEATY
TVLPRGTAPS EVSLNSTTPS SRIVKLHLPS SNAERHAKLK RGTARKATPF
PPVISNAVFV YLPFVLLLLL FRGRALYLVT NA
>HEPHAESTIN-2 PRECURSOR,PETROMYZON MARINUS
MPLCARAAVV AALAALLVGR VRARDREFHV AVREVDWDYA PGGGSGVSPE
QGHAHMYLSQ GPARIGSVYR KAVFREYTDS SFSLESPRPA WLGLLGPVLR
AEVGDVLRVN LRNLASRPYG LHPHGVFYDK ASEGARYPDG TSGDDELDEA
VPPGGRHTYL WEVREEFGPT ADDPACLTWA YHSHMDASRD IASGLAGALL
TCKRGILDPV TSKRRDVDQE FVFMFAIVNE NLIWYLGKNI QRFCGSPDSV
DTEDEGFVES NQMHAINGLV FGHLPGLEMC VGERVAWHLL AIGGEKDLHT
ARFHGQTLVL TGGVRIDTVD LFAASFASGD AVPTEFGSWL LTCGVNHHVH
AGMQALYTVR DCGRAAPAPA AGGETRRYFV AAEESVWDYG PSGVDAFTGK
RLDDPETESA TFFERGPTRL GGRYKKARFV GYTDASFTQQ TARHAGEQHL
GLMGPVLRAQ VGDTVVVTFV NRATRPYSIQ PHGLLVTKDN EGIVYTDGRS
AQRRASHVPP GGNFTYTWAV PRSAGPAASD PDCLAWMYFS AVDSIRDTNA
GLLGPLLVCR PEALEPSGKQ KAFDEEFFLL YTVFDENESW YLDDNIEHFA
GNASAVDKED ADFQESNKMH AINGFMFGNQ PGLSVCQGGR VSWSFLGVGN
EVDMHGVFVL GNTLLAGGRR IGSLGLFPHV ARTASMVARV GKFEVGCQTA
DHYLAGMKQF YSVSRCSRPG TADPGGSRRR RVYYLAAREV DWDYFPDDTW
EKERHGDNPE AREAAKFTAR GDERIGSVYK KAVYLQYEDA TFTKLRGRGP
DEEHLGILGP VLRAEVGDEL TVVFRNEASR PYSVHAHGVE AAVGDTTATD
PGGKTHTYRW EVTEASGPGP SDPNCVTWTY YSRVDPVKNL YSGLHGPLVV
CEAGVLGAEG RRADVDREFA LLFLIYDENL SWYLEDNVRT FCVHPERVDT
SDEGFKESNR MHAINGWLYG NARGLRMYRG EKVAWYLLGM GNEVDIHTVH
FHGETFLYKK RHDHRGDVHE LFPGTSDSVE MRPRNPGTWL LHCHLTEHIY
AGMETTYHIL EGKEYANHHK HLLSVAPIHC ASNPCMSGGA LRPMTAAGVT
ILALLPALAA VADGGTNSI
>HEPHAESTIN-2 L. JAPONICUM (SCAFFOLD 00078,CONTIG476361)a
DREFHVAVRE VDWDYAPGGG SGVSPEQGHA HMYLSQGPAR IGSVYRKAVF
REYTDSSFSL ESPRPAWLGL LGPVLRAEVG DVLRVNLRNL ASRPYGLHPH
GVFYDKRSEG ARYPDGTSGD DKLDDAVPPG GRHTYVWHVR EEFGPTADDP
ACLTWAYHSH VDASRDIASG LVGALLTCKR GILDPVTSKR RDVDQEFVVM
FAIVNENLSW YLGKNIQRFC GSPDSVDTED EGFVESNQMH AINGLVFGHL
PGLEMCVGER VAWHLLAIGG EKDLHTARFH GQTLSLTGGV RVDTVGLFAA
SFASGDAVPT EVGAWLLTCG VNHHVHAGMQ ALYTVRDCGR AAPAPASGGE
TRRYFVAAEE SVWDYGPSGV DAFTGKRLDD PETESATFFE RGPTRLGGRY
KKARFVGYTD ASFAQKTVRQ AGEQHLGLLG PVLRAQVGDT VVVTFVNRAT
RPYSIQPHGL LVTKDNEGIA YTDGRSAGRR TYPPGGNFTY TWAVPRSAGP
AASDPDCLAW MYFSAVDSIR DTNAGLLGPL LVCRPEALEP SGKQKAFDEE
FFLLYTVFDE NESWYLDDNI ERFAGNASAV DKEDAGFQES NKMHAINGFM
YGNQPGLSAC QGGRVSWSFL GVGSEVDMHG AFVLGNTLLA GGRRIGSLGL
FPHVARTASM VARVGKFEVG CQTADHYLAG MKQFYSVFRC SRPGTAHPGG
ARRQRVYYLA AREVDWDYFP DDTWEKERHG DNPEARDAAK FTARGDERIG
SVYKKAVYLE YEDATFTKLR ARRPDEEHLG ILGPVLRAEV GDELIVVFRN
GASRPYSVHA HGVDAAGNDT TATDPGGKTH TYRWEVTEAS GPGPSDPNCV
TWTYYSRVDP VKDVYSGLFG PLVVCEAGVL GAEGRRADVD REFALLFLIF
DENLSWYLQD NVRAFCVHPE RVDTSDEGFK ESNRMHAING WLYGNARGLR
MYRGETVAWY LLGMGNEVDI HTVHFHGETF LYKKRHDHRG DVHELFPGTS
DSVEMRPRNP GTWLLHCHLT EHIYAGMETT YHILEGKEYT NHHKHLLSVA
SIHFASNPCV SGGALRPVTA VGMTILALLM ALAVVADRST NTVASSSWFF
R
PROTHROMBIN, PETROMYZON MARINUS
VFLDRPVASR VLHPRTRRAN SFLEERLQGN LERECVEETC SHEEAREVYE
NDAGTVRPHN GADGCNLDNI RSHMELFRGC LKGLCMTGNG EQYIGNVSFT
KSGRQCQLWT SNFPHQVRFK PVDHPEKQLV ENFCRNPDAS QDGPWCYTRD
PLVPREPCSV PTCGEDPNQH VPVSPRVTPT KPAPRPQGPC VPGAGEDYRG
PVNVTRSGRH CQAWSAQTPH VSRYSPLTHR AAGLEGAWCY TTDPGLTIDY
CALHYCDEDD IFMEAPKEEG GRGRTTATGT DPKMTLINPR SFGSGQLECG
KRPMFELMAK EDVGEEELQE SQRQRVVHGD NAEEGSAPWQ VMLYRKSPMD
MLCGASLISD QWVLTAAHCV FYPPWDKNFT AKELAVRIGK HNKARYEKDR
EKISNVDKII IHSKYNWKEN MERDIALLHL DKPITFTQYI VPICLPTRDV
AVSLLKSGFK GRVTGWGNLF QTWTNNPRAH PKVLQMINLP IVDPNRCQAS
TTHRITANML CAGYEPEDVK RGDACEGDSG GPFIMKDFES KRWYQMGIVS
WGEGCDRDGK YGIYTHVYRL RKWINKVIGE PD
>PROTHROMBIN PRECURSOR [LETHENTERON JAPONICUM] (SCAFFOLD 00194)a,c
MIVAVVMATV AMAMIHATQG AQVFLDRPVA SRVLQPRTRR ANSFLEERLQ
GNLERECLEE TCSHEEAREV YENDAGTAAF WNQYKGCNVD NIRSHMELFR
RCLKGLCITG NGEQYIGNVS ITKSGRQCQL WTSNFPHQVR FKPVDHPEKQ
LVENFCRNPD ASQDGPWCYT RDPLVPREPC SVPSCDEDPS RHVPELPRVP
PTAPATRPQG PCVPGAGEDY RGPLNVTRLG RRCQAWSART PHVSRFSPST
HSAAGLEGAW CYTTDPGLAV DYCALHYCDE DDIFMEAPKE EGGRGRTTAT
GTDPKMTLIN PRSFGSGQLE CGKRPMFELM GKEDVGEEEL QESQRQRVVH
GDNAEEGSAP WQVMLYRKSP MDMLCGASLI SDQWVLTAAH CVFYPPWDKD
FTAKELAVRI GKHNKAGYEK DREKISNVDK IIIHSKYNWK ENMERDIALL
HLEKPITFTQ YIVPICLPTR DVAVNLLKSG FKGRVTGWGN LFQTWTNNPR
AQPKVLQMIN LPIVDPSRCQ ESTTHRITAN MLCAGYEPED VKRGDACEGD
SGGPFIMKDF ENKRWYQMGI VSWGEGCDRD GKYGIYTHVY RLRKWINKVI
GEPDT
>COAGULATION PROTEIN C, PETROMYZON MARINUS (CONTIGS 37541 AND 12525)b,d
PHRYRRAKPV REILEEIRPG SLERECVEET CTMEEVHEIT GDTTDGDQCA
SSPCQKHATC HDRKGRYICT CDEGWEGINC ELGENSSTCA EDNGGCDHFC
LPADDKGSRK CECAAGYTLA PDGMACPPVD IPCGKILVTL TTGQANHTAV
TEPERGPFLT PRITGGQICT KGHCPWQVLL IKGTNKAYCG GTLIHQQWVL
TAAHCVSVQE KYPTFTGDHY RNKPEPEERN VAVAEIIAHP HFNLRTFYND
IALLRLRNAV GLSPYIVPAC LPDPRAAERS LLLPGVRAVV SGWGRTLEQP
GFSGVLRFAR VSLVSHEACI NTSRFAVTRD MLCAGPGAPQ ADACDGDSGG
PLVAPLVARG RDRTWFVIGL VSWGEGCALP GKYGIYTRVS RYVLWIESSI
RAAEAGRVGG SRGA
>COAGULATION PROTEIN C, LAMPREY (LETHENTERON JAPONICUM) (SCAFFOLD 00651)a
HRNRRAKPVR EILEEIRPGS LERECVEETC TMEEVHEITG DTTDGDQCAS
SPCQNNATCH DRKGLYICTC DEGWEGINCE LGENSSECAE DNGGCDHFCL
PAEDKGSRKC ECAAGYTLAP DGMACPPVDI PCGKIQVASM TGQANHTTVA
EPERGPFLTP RITGGQICTK GHCPWQVLLI KGTNKAYCGG TLIHQQWVLT
AAHCVSVQEK YPTFVGDHYR NKPEPEERTV AVAEIIAHPH FNLRTFYNDI
ALLRLRHAAG LSPYIVPACL PDPRAAERSL LLPGVRAVVS GWGRTLEQPG
LSGVLRFARV SLVSHETCIN TSRFAVTRDM LCAGPGAPQA DACDGDSGGP
LVAPLVARGR DRTWFVIGLV SWGEGCALPG KYGIYTRVSR YVLWIESSIR
AAEAGRVGGS RGA
>COAGULATION FACTOR XA, PETROMYZON MARINUS (SCAFFOLD 477025 2012 ASSEMBLY)b,d
DTHGFLLRPI RPPPVVGAAP LQATVFLQRR QAAIVLARAR RANSGWEELR
KGNLERECME ERCSFEEARE VFENIDATDG DQCSSNPCKN NGVCKDGINE
YTCYCLSGYD GNTCEIAKLG CTFRNGGCQH FCQGSDEQFS CSCAKDYKLH
ADGKSCQPVV PFPCGRIQVQ VKKPRSKREA SSVTVEKPVQ LVPPNSTVSD
NSSYIAEGSE GDLRIAGGQE CPLGECPWQV LLLDKKGEGF CGGTILSREW
VLTAAHCIPS EPDIVIVGEH NRTVSEPTEQ TISIKQIVMH NRFNNATYDN
DIALLQMSEP IKFNKYVLPA CLPEPDFADN VLKEELARIS GWGYLRERGL
KAKVLQTSFV PYQDMARCKE SSSYTITKNM FCAGYSDSKT DACQGDSGGP
HVTPYANTWF STGIISWGEG CNRKGKFGIY ARVSRYLPWI DTVMKNYSVY
KDEIPTAGRN LRRSRQN
>COAGULATION FACTOR XA1, LETHENTERON JAPONICUM (SCAFFOLD 00078)a,c
DTDGFLLRPK RPPPVVGAAT LQACHANVFL QRRQAAIVLA RARRANSGWE
ELRKGNLERE CMEERCSFEE AREVFENIDA TNDFWNKYSD GDQCSSNPCK
NNGVCKDGIN QYTCYCLSGY DGNTCEISKL GCTFRNGGCE HFCQGSDEQF
SCSCAKDYKL LDDGKSCRPM VPFPCGRIQV QVKKPRSKRE ASSVTVATPV
QSVPPHSTVS DNSSYIAEGS EGDLRIAGGE ECPLGECPWQ VLILDKKGEG
FCGGTILNRE WVLTAAHCIP SEPDIVIVGE HNRTVSEPTE QQISIKQMVM
HNRFNNATYD NDIALLQMSE PIKFNKYVLP ACLPEPDFAD NVLKEELARI
SGWGYLRERG LKAKVLQTSF VPYQDMARCK ESSSYTITKN MFCAGYSDSK
TDACQGDSGG PHVTPYANTW FSTGIISWGE GCNRKGKFGI YARVSRYLPW
IDTVMKNYSV YKDEIPTAGR NLRRSRQN
>COAGULATION FACTOR XA2, LETHENTERON JAPONICUM (SCAFFOLD 00078, GENE 2)a
DTDGFLLRPK RPPPVVGAAP LQCVATVFLQ RRQAAIVLAR ARRANNGLEE
LPKGNLEREC MEERCSFEEA REVFENIDAT NEFWNKYLDG DQCSSNPCKN
NGVCKDGINQ YTCDCLSGYD GNTCEIAKLG CIFRNGGCEH FCQGSDEQFS
CSCAKDYKLL DDGKSCQPVV PFPCGRIQVQ VQKPRSKREA SSVTVETLVQ
PVPPHSTVSD NSSYIAEGSE ADLRIAGGEE CPLGECPWQV LILDKKGEGI
CGGTILNREW VLTAAHCIPY EPDIVIVGES NRTVSEPTEQ KISIKQMAMH
NRFSNATYDN DIALLQMSEP IKFNKYVLPA CLPEPDFADN VLEEELARIS
GWGILGKRRL KAEVLQTSFV PYQDMARCKE SSSYTITKNM FCAGYSDSKT
DACQGDSGGP HVTPYANTWF STGIISWGEG CNHKGKFGIY ARVSRYLPWI
DTVMKNYSVY KDEIPTAGRN LRRSRQN
>FACTOR 10B, P. MARINUS (SCAFFOLD 477154, 2015 ASSEMBLY)b
MALPSATFVL LIGSVIPGTL CAVFLEKQDA GQVLSRLRRA NSAFEELRAA
NLERECNEEP CSIEEAMEIF KNKELVNDFW NTYVDGNQCD PNQCLNNGIC
KDGVKMYTCI CPNGFEGNNC ELSKIGCVFR NGGCNHFCLD QENKAPVCTC
VKGYRLQDDG HTCEPVEAFP CGRLHPEEIR AKREENRQDE FFTSHSDDNL
KAQPHNTSSI KEEREDVKTI AGGMDCPRGD CPWQALVKLE NNLYCGGTIL
NLEWILTAAH CLPLKPWNVV VGEHNLGVTE SSEQHVPIKN IVVHSRYDPI
TFDNDIALLQ LERPLNFSRL VLPACLPERD FADKVLVEEQ SRISGWGSLH
HRGIKQATVL QIAEVPFVDT LRCKESSSSL VTKNMFCAGY SDGTKDACQH
DSGGPHVTLF KDTWFATGII SWGEGCARKG KYGVYTRVSR YLLWMHQVMQ
RASVYKLEIP TRGIRFTRHP LA
>COAGULATION FACTOR XB LETHENTERON JAPONICUM (SCAFFOLD 00705)a,c
MALSSATFVL LIGSIIPMTL CAVFLEKQDA GQVLSRLRRA NSAFEELRAA
NLERECNEER CSIEEAMEIF KNKELANDFW NTYVDGNQCD PNQCLNNGIC
KDGVKMYTCI CPNGFEGNNC ELTKIGCVFR NGGCNHFCLD QENKAPVCTC
VKGYRLQDDG HTCEPVEAFP CGRLHPEEIR AKREENRRDE FSTSHSDENL
KTQPHNTSSI KEQREDVKSI AGGMDCPRGD CPWQALVKLE NNLYCGGTIL
NLEWILTAAH CVPLKPWNVV VGEHNLRVTE SSEQHVPIKN IVVHSRYDPI
TFDNDLALLQ LERPLNFSRL VLPACLPERD FADKVLVEEQ SRISGWGSLH
HRGIKATVLQ IAEVPFVDTL RCKESSSSLV TKNMFCAGYS DGTKDACQHD
SGGPHVTIFK DTWFATGIIS WGEGCARKGK YGVYTRVSRY LLWMHQVMQR
ASIYQLEIPT RGIRFTRNPL A
>FACTOR 7A, PETROMYZON MARINUS (CON2959, NEWTRACES)(GAP BASED ON L. JAPONICUMa)
VFVSRLEAAS LLARQRRANS PFSMEETKQG SLERECREET CNFEEAREIF
EDDQRTNAFW TLYISPNPCL SNPCQNGGTC QHTHTVYMCV CPRGWEGRVC
SQEVSTGQSC KVNNGRCDHY CEEEEAAAAG GARRGAVCSC APGYHLARND
RVCATAEAFP CGRVVSPKSE ILMGNSSEAA THVPPSAHAA NVNSSSIGDD
SEPPASFGQS NHRGRVVGGK ECPRGQCPWQ VLISRKGAPV CGGTILSPQW
ILTAAHCTIY ETKNLRILAG ------
------GVLRSVVA GTVSGWGKLN
EFGPAAGILQ RLEVPYVDDE QCRAAMGSKV ITANMFCAGY ETGGQDSCSG
DSGGPHATRY GSTWFLTGVV VSWGKGCARE GKFGVYTKVL NYLDWIAQTM
AEAESHTLPE PAQH
>COAGULATION FACTOR VIIA, LETHENTERON JAPONICUM (SCAFFOLD 00301)a,c (MATURE)
VFVSRLEAAS LLARSRRANS PFSMEETKQG SLERECREET CNFEEAREIF
EDDQRTNAFW TLYIRPNPCL SNPCQNGGTC QHTHTVYMCV CPRGWEGRVC
SQEVSTGQSC KVNNGRCDHY CEEEEAAAGG RRGAVCSCAP GYHLARNRRS
CMAMEAFPCG RVASPKSDIL MGNSSEAATD VPTPTSQAAN VNSSSIIGDD
SEPQASFGHS NHRGRVVGGK ECPRGQCPWQ VLISRKGAPV CGGTILSPQW
ILTAAHCTIY ETKNLRILAG EHNVDEVEGS EQELGILRIV DHPSFNIKLS
YDNDISLIQL SRPIQFTRYA LPICLPNQRF VRGVLRSVVA GTVSGWGKLN
EFGPAAGILQ RLEVPYVDDE QCRAAMGSKV ITANMFCAGY ETGGQDSCSG
DSGGPHATRY GSTWFLTGVV SWGKGCAREG KFGVYTKVLN YLDWIAQTMA
ETESHALPEP AQH
>FACTOR VIIB, PETROMYZON MARINUS (PARTIAL, GAPS BASED ON L. JAPONICUMa)
------
------APAP RVTPCRPNPC KNGGRCLELR SAARCVCAEG WEGDTCHVGD
ESPRRRSCER NNGGCEHYCE ASRIYRLVCH CARGYQLSDN GFSCDQIGGT
PDVMRIVGGY ICPRGHCPWQ V------
------IPGEHDVRL TEGSEQNLPA NLIVPHPNFD SDTFDNDLAL
LRLNSSARLD RYAVPACLPD RRLAELLTQP GTLGVVSGWG REQEHGRNSR
YLQWVGLPVV PTATCTQSTQ KVTQNMFCAG YAAGRQDACK GDSGGPYMSR
RLDTWFLTGV TSWGEGCAQR GKYGAFTRLA NFIDWIDATM RENTS
>COAGULATION FACTOR VIIB LETHENTERON JAPONICUM (SCAFFOLD 00131)a
VFVDPRRAAL VLRRTRRKNS DFEELRPGSI ERECREETCS FEEAKEAVGH
EQAVRGATTP RVTPCRPNPC KNGGRCLELR SAARCVCAEG WEGDTCHVGE
ESSRRRSCDR NNGGCEHYCE ASRIYRLVCH CARGYQLSDN GFSCDQIGGT
PDGMRIVGGY ICPRGHCPWQ VLLTDRRGNG FCGGTLVSAR WVVTAAHCAR
YTKQVLVRIG EIPGEHDVRL TEGSEQNLPA NLIVPHPNFD SDTFDNDLAL
LRLNSSARLD RYAVPACLPD RRLAELLTQP GTLGVVSGWG REQEHGRNSR
YLQWVGLPVV PTATCAQSTE QVVTQNMFCA GYAAGRQDAC KGDSGGPYMS
RRLDTWFLTG VTSWGEGCAQ RGKYGAFTRL ANFIDWIEAT MRENAS
>COAGULATION FACTOR VIIC, PETROMYZON MARINUSb,dCHIMERA (ITALICIZED RES ARE FROM LJ-SCAF00627a)
VFVSRREASR LLRVRRVNDH GLEEFLPGSL ERECMEEHCS LEEAMEIFPD
KEAAVSIETP HTTKDGDQCA SSPCHAGACK DALNSYSCVC PDGYQGVNCE
ISEAADTRTC GYRNGGCEHF CWERGDNGTV LCGCAEGYAL RDNGTCSPQG
GRSGGDDLRIVNGWRCEPGECPWQVLLVSY ETGKGLCGGA LLNDEWVITA
AHCIVYVAGE YDVDVLDGSE QYLPVRRVVL HKRHVAPSRD YDVALLQLAT
RARLGPSAVP LCLPSTSLSE QQLLMPGIHG LVSGWRLGEG AARASVLQAV
SVPFVPHAEC VRALGSAVTR NMFCAGRSAG RGDACGGDSG GPHATLYGDT
FFLTGIVSWG DGCARSGKYG VYTKVSRFVP WIREVMGRPE VKA
>FIBRINOGEN GAMMA CHAIN, LAMPREY (PETROMYZON MARINUS)e
QVRDLKQCSN DPEFGRYCPT TCGVADVLSK YAKGVDEDSS FIDSVLTQLA
AKHGIVEGNV NIVNEDVRIT RDEAQIIKDS GQKTVQKILE EVRILEQIGV
SHDAQIQELS EMWRVNQQFV TRLQQQLVDI RQTCSRPCQD TTANKISPIT
GKDCQQVVDN GGKDSGLYYI KPLKAKQPFL VFCEIENGNG WTVIQHRHDG
SVNFTRDWVS YREGFGYLAP TLTTEFWLGN EKIHLLTGQQ AYRLRIDLTD
WENTHRYADY GHFKLTPESD EYRLFYSMYL DGDAGNAFDG FDFGDDPQDK
FYTTHLGMLF STPERDNDKY EGSCAEQDGS GWWMNRCHAG HLNGKYYFGG
NYRKTDVEFP YDDGIIWATW HDRWYSLKMT TMKLLPMGRD LSGHGGQQQS
KGNSRGDN
>FIBRINOGEN GAMMA CHAIN, L. JAPONICUM (SCAFFOLD 00040)a (PARTIAL)
QVRDLKQCSN DPEFVIKDSG QKTVQQILEE VRILEQIGVS HDAQIQELSE
MWRVNQQLVT RLQQQLVDIR QTCSRSCQDT TANKISPITG KDCQQVVDNG
GKDSGLYYIK PLKAKQPFLV FCEIENGNGW TVIQHRHDGS VNFTRDWVSY
REGFGYLAPT LTTEFWLGNE KIHLLTGQQA YRLRIDLTDW ENTVRYADYG
HFKLTPESDE YRLFYSMYLQ DKFYTTHLGM LFSTPERDND KYEGSCAEQD
GSGWWMNRCH AGHLNGKYYY GGKYRKADVE YPYDDGIIWA TWHDRWYSLK
MTTMKLLPMG RDLSGHGGQQ QSKGNSRGDN
>FIBRINOGEN BETA CHAIN, PETROMYZON MARINUSf
EDLSLVGQPE NDYDTGDDTA ADPDSNNTAA ALDVRGVRPL PSGTRVRRPP
LPPPASGSGR RYEPRPTSVP KAGQEAQKAI RDEGGCMLPE SDLGVLCPTG
CELREELLKQ RDPVRYKISM LKQNLTYFIN SFDRMASDSN TLKQNVQTLR
RRLNSRSSTH VNAQKEIENR YKEVKIRIES TVAGSLRSMK SVLEHLRAKM
QRMEEAIKTQ KELCSAPCTV NCRVPVVSGM HCEDIYRNGG RTSEAYYIQP
DLFSEPYKVF CDMESHGGGW TVVQNRVDGS SNFARDWNTY KAGFGNIAFD
NGKSICNIPG EYWLGTKTVH QLTKQHTQQV LFDMSDWEGS SVYAQYASFR
PENEAQGYRL WVEDYSGNAG NALLEGATQL MGDNRTMTIH NGMQFSTFDR
DNDNWNPGDP TKHCSREDAG GWWYNRCHAA NPNGRYYWGG IYTKEQADYG
TDDGVVWMNW KGSWYSMRQM AMKLRPKWP
>FIBRINOGEN BETA CHAIN LETHENTERON JAPONICUM (SCAFFOLD 00050)a (PARTIAL)
LDVRGMRPMP SGTRVRRPPL PPPASGSGRR YEARPTSAPK AGLEAQKAIR
DEGGCMLPES DLGVLCPTGC ELREELLKQR DPVRYKISML KQNLTYFINS
FDRMAADSNT LKQNVQTLRR RLNGERRSTH VDAQKEIENR YKEVKIRIES
TVAGSLRSMK SVMEHLRAKM QRMEEAIKTQ KELCSAPCTV NCHVPVVSGM
CEDIYRNGGR TSEAYYIQPD LFSKPYKVFC DMDSHGGGWT VVQNRVDGSS
NFARDWNTYK AGFGNIAFDN GKSICNIPGE YWLGTETVHQ LTKQHTQQVL
FDMSDWEGSS VYAQYASFRL ENEAQGYRLW VEDYSGNAGN ALLDGATQLM
GDNRTMTIHN GMQFSTFDRD NDNWNPGDPT KHCSREDAGG WWYNRCHAAN
PNGRYYWGGI YTKEQADYGT DDGVVWMNWK GSWYSMRQMA MKLRPKWP
>FIBRINOGEN ALPHA CHAIN LAMPREY (PETROMYZON MARINUS) (RES 1-219)g
DDISLRGPRL TEQRSAGQGS CASATADLCV HGDWGRKCPN GCRMQGLMSH
AEKDIGKRIG DLTERLARLG RLYTQVHTDF RAVSDTSGQT LNEHNELEVR
YSEVLRELER RIIHLQRRIN MQLQQLTLLQ HNIKTQVSQI LRVEVDIDVA
LRACKGSCAR YLEYRLDKEK NLQLEKAASY IANLKFERFE EVVVEETLNR
RVETSSHAFQ PTHGQGTPQ
>FIBRINOGEN ALPHA CHAIN LETHENTERON JAPONICUM (RES 1-219) SCAFFOLD 00123a
EEISLRGPRL TEQRAAGQGS CASATADLCV HGDWGRKCPS GCRMQGLMTR
TETDIGKRIG DLTERLARLG RLYTQVHTDF RAVSDTSGQT LNEHSELEVR
YSEVLRELER RIIHLQRRIN MQLQQLTLLQ HNIKTQVSQI LRVEVDIDVA
LRTCKGSCAR YMEYRLDKEK NLQLEKAAGY IANLKFDRFE EVVVEETLNR
RVETSSHTFQ PTPSNVGPR
>TISSUE FACTOR, PETROMYZON MARINUS
MESVTTCVAV TWLLLMACAV PTNLSFVSVN FKTLLEWQVE DTGIPVTYDT
WTTWEQKAEC TDSIETTCDL TSIMADPRLS YRARVRAIPG GSSAVHTVPW
VMSPSFVPYA ELGKPQFEAV GRNGSIQMKV ADLLTPYFNI DGSHKTMGDI
YKGDLHYHLT YWKDGSSGRV GGTLTGLDEG QLYCFHVQLL VDADNTERRG
EESDVKCVRS GIAKSLLDYA WIFLVAAGIT ALIIGVALIV LIVWLCHRCR
QQRKSERN
>TISSUE FACTOR, LETHENTERON JAPONICUM (SCAFFOLD 00026)a
MESVTTRVAV TWLLLMACAV PTNLSFVSVN FKTLLEWQVE DTGIPVTFDT
WPTWKQKAEC TDSIETTCDL TSIMADPRLS YRARVRAIPG GSSAVHTVPW
VMSPSFVPYA ELGKPQFEAV GRNGSIQLKV ADLITPYFNI DGSHKTMGDI
YTGDLRYHLT YWKDGSSGRV GGMLTGLDEG ELYCFHVQLL VDVDNAERRG
EESDVKCARS GLAKSLLDYA WIFLVAAGIA ALIIGVALIV LIVWLCHRCR
QQRKSERN
DNA SOURCES
aMehta et al, 2013
bSmith et al, 2013
cKimura et al, 2013 (cloned)
dDoolittle et al 2008
eBohonus et al 1987 (cloned)
fStrong et al 1985 (cloned)
gWang et al (1989) (cloned)
REFERENCES
Bohonus VL, Doolittle RF, Pontes M, Strong DD (1986) Complementary DNA sequence of lamprey fibrinogen chain. Biochemistry 25: 6512-6516
Doolittle RF, Jiang Y, Nand J (2008) Genomic evidence for a simpler clotting scheme in jawless vertebrates. J Mol Evol 66:185-196
Kimura A, Ikeo K, Nonaka M (2009) Evolutionary origin of the blood complement and coagulation systems inferred from liver EST analysis of lampreys. Dev Comp Immun 33:77-87
Mehta TK, Ravi V, Yamasaki S, Lee AP, Lian MM, Tay B-H, Tohari S, Yanai S, Tay A, Brenner S, Venkatesh B (2013) Evidence for at least six Hox clusters in the Japanese lamprey (Lethenteron japonicum). Proc Natl Acad Sci, USA 110:16044-49.
Smith JJ and 58 other authors (2013) Sequencing of the sea lamprey (Petromyzon marinus) genome provides insights into vertebrate evolution. Nat Genetics 45:415-421
Strong DD, Moore M, Cottrell BA, Bohonus VL, Pontes M, Evans B, Riley M, Doolittle RF (1985) Lamprey fibrinogen chain: cloning, cDNA sequencing and general characterization. Biochemistry 24: 92-101
WangY-Z, Patterson J. Gray JE, Yu C, Cottrell BA. Shimizu A, Graham D, Riley M, Doolittle RF (1989) Complete sequence of the lamprey fibrinogen chain. Biochemistry 28: 9801-9806