Competitive EIA Questionnaire
Product Name:
Product number:
Lot Number:
Kit size (96 well or 480 well):
Date Received:
Temperature upon receipt:
Stored at (room temperature, 4 C, - 20 C, - 80 C):
Date first used:
Storage after reconstitution, if applicable:
Description of compliant:
Please attach data in an Excel format, the Cayman EIA Workbook, or Softmax Pro software
Were the kit components in the kit box used together (no substitution of older components)?
Was Ultrapure water that was deionized and free of trace organic contaminants (˂10 ppb) used to prepare EIA buffer, or Immunoassay Buffer, and Wash buffer?
Was the buffer provided in the kit used for the assay? If not what buffer was used?
Were the tracer and antiserum reconstituted with EIA buffer? If not what was used for reconstitution?
What volume of liquid was used for reconstitution of the tracer and antiserum?
After reconstitution how long were the tracer and antiserum stored and at what temperature?
Were the antiserum and tracer dyes added?
If dyes were used, how long were the antiserum and tracer stored following the addition of the dyes?
Were the kit components brought to room temperature prior to incubation?
What volume of tracer and antiserum were applied to the wells?
What was the length of time and temperature for incubation?
If the assay has a one hour or two hour incubation, was that incubation performed on an orbital shaker?
Was the assay run as directed in the kit booklet, with a complete set of controls including standards 1 – 8 in duplicate? If not what control were run?
Was the Ellman’s reagent reconstituted with Ultrapure water and used within 30 minutes of preparation.
Was the plate on an orbital shaker for development?
How long was the plate developed?
What was the raw absorbance value for the Blank? (Many sets of data are blank subtracted, please ignore this question if the raw data includes the Blank value).
What sample type was used?
(Plasma, serum, cell lysate (identify cell type and buffer used to process), tissue homogenate (identify tissue type and buffer use to process), cell culture medium (describe medium),
If cell culture medium was the sample type were the standards diluted with medium or with EIA buffer?
Were the samples purified? If so, was the kit booklet purification protocol employed?
What sample dilutions were performed? What was used (buffer, water) to make the dilutions?
Again, please attach data in an Excel format, the Cayman EIA Workbook, or Softmax Pro software